Development of a heterologous human CYP2C9-NADPH P450 reductase system: An in vitro enzyme system for drug metabolism study / Riza Afzan Asri by Asri, Riza Afzan

“…Recombinant CYP2C9 and NADPH-Cytochrome P450 reductase were co-expressed in separate but compatible plasmid to generate an active in-vitro drug metabolizing system. …”
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Identification of the domains of neuronal nitric oxide synthase by limited proteolysis. by Lowe, P, Smith, D, Stammers, D, Riveros-Moreno, V, Moncada, S, Charles, I, Boyhan, A

“…The protein consists of a central calmodulin-binding sequence flanked on the N-terminal side by a haem-binding region, analogous to cytochrome P-450, and on the C-terminal side by a region homologous with NADPH:cytochrome P-450 reductase. The structure of recombinant rat brain nitric oxide synthase was analysed by limited proteolyis. …”

Agrobacterium-Mediated Transformation of Chrysanthemum with Artemisinin Biosynthesis Pathway Genes by Aleksey Firsov, Tatiana Mitiouchkina, Lyubov Shaloiko, Alexander Pushin, Alexander Vainstein, Sergey Dolgov

“…Chrysanthemum (<i>C. morifolium</i> Ramat.) was transformed by <i>Agrobacterium tumefaciens</i> carrying with the binary vectors p1240 and p1250, bearing artemisinin biosynthesis genes coding: amorpha-4,11-diene synthase, artemisinic aldehyde Δ11(13) reductase, amorpha-4,11-diene monooxygenase (p1240 was targeted to the mitochondria and p1250 was targeted to the cytosol), cytochrome P450 reductase from <i>A. annua</i>, as well as yeast truncated 3-hydroxy-3-methylglutarylcoenzyme A reductase. …”
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Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury by Erik Lidin, Mattias K. Sköld, Maria Angéria, Johan Davidsson, Mårten Risling

“…In this study, we aimed to describe hippocampal CYP1B1 mRNA expression, protein presence of CYP1B1 and its key redox partner Cytochrome P450 reductase (CPR) in both sexes, as well as the effect of penetrating traumatic brain injury (pTBI). …”
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Transcriptomic and metabolomic analyses reveal the antifungal mechanism of the compound phenazine-1-carboxamide on Rhizoctonia solani AG1IA by Ya Zhang, Qiufeng Li, Chong Wang, Shuangqing Liu

“…The molecule docking indicted that NADPH nitrite reductase, ATP-binding cassette transporter, alpha/beta hydrolase family domain-containing protein, and NADPH–cytochrome P450 reductase maybe the particular target of PCN. …”
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CYP-Dependent Metabolism of PF9601N, A New Monoamine Oxidase-B Inhibitor, by C57BL / 6 Mouse and Human Liver Microsomes by Stefania Dragoni, Giada Materozzi, Federica Pessina, Maria Frosini, José Luis Marco, Mercedes Unzeta, Giampietro Sgaragli, Massimo Valoti

“…CYP activities, cytochrome b5 content, NADPH-cytochrome P450 reductase and various monooxygenase activities were unaffected by in vivo PF9601N treatment. …”
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Rewiring Saccharomyces cerevisiae metabolism for optimised Taxol® precursors production by Behnaz Nowrouzi, Pablo Torres-Montero, Eduard J. Kerkhoven, José L. Martínez, Leonardo Rios-Solis

“…However, the harmful impact of oxidative stress by the first cytochrome P450-reductase enzymes (CYP725A4-POR) of Taxol® pathway has hampered sufficient progress in yeast. …”
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Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization in <i>Pichia pastoris</i> Yeast by Vyacheslav Kollerov, Sergey Tarlachkov, Andrei Shutov, Alexey Kazantsev, Marina Donova

“…A two-gene construct with a candidate P450_cur gene and the gene of its natural redox partner, NADPH-cytochrome P450 reductase (CPR), which is interconnected by a T2A element, was created. …”
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Comparative gut proteomics study revealing adaptive physiology of Eurasian spruce bark beetle, Ips typographus (Coleoptera: Scolytinae) by Muhammad Zubair Ashraf, Kanakachari Mogilicherla, Gothandapani Sellamuthu, Valentina Siino, Fredrik Levander, Fredrik Levander, Amit Roy

“…Gut enzymes such as NADPH-cytochrome P450 reductase (CYC), glutathione S-transferase (GST), and esterase (EST) play a crucial role in the I. typographus for detoxification and digesting of host allelochemicals. …”
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Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice by Jiang-Wei Zhang, Yong Liu, Jie Cheng, Wei Li, Hong Ma, Hong-Tao Liu, Jie Sun, Li-Ming Wang, Yu-Qi He, Yun Wang, Zheng-Tao Wang, Ling Yang

“…To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome P450 reductase (CPR). Methods. The inhibitory effects of star fruit juice (0.5 to 5%, v/v) against the activities of seven CYP isoforms including CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4 and CPR were examined in human liver microsomes. …”
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High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system by Jutapat Romsuk, Shuhei Yasumoto, Shuhei Yasumoto, Ery Odette Fukushima, Ery Odette Fukushima, Kenji Miura, Toshiya Muranaka, Toshiya Muranaka, Hikaru Seki, Hikaru Seki

“…We also demonstrated the efficacy of co-expressing a mutant Arabidopsis thaliana HMGR1 catalytic domain, additional NADPH-cytochrome P450 reductase (CPR) transferring electrons to heterologous CYPs, and application of ascorbic acid for preventing leaf necrosis after agroinfiltration, to improve product yield. …”
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The Multienzyme Complex Nature of Dehydroepiandrosterone Sulfate Biosynthesis by Anastasiya Tumilovich, Evgeniy Yablokov, Yuri Mezentsev, Pavel Ershov, Viktoriia Basina, Oksana Gnedenko, Leonid Kaluzhskiy, Tatsiana Tsybruk, Irina Grabovec, Maryia Kisel, Polina Shabunya, Natalia Soloveva, Nikita Vavilov, Andrei Gilep, Alexis Ivanov

“…Dehydroepiandrosterone (DHEA), a precursor of steroid sex hormones, is synthesized by steroid 17-alpha-hydroxylase/17,20-lyase (CYP17A1) with the participation of microsomal cytochrome b5 (CYB5A) and cytochrome P450 reductase (CPR), followed by sulfation by two cytosolic sulfotransferases, SULT1E1 and SULT2A1, for storage and transport to tissues in which its synthesis is not available. …”
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Promoting Photosynthetic Production of Dammarenediol-II in <i>Chlamydomonas reinhardtii</i> via Gene Loading and Culture Optimization by Mei-Li Zhao, Xiang-Yu Li, Cheng-Xiang Lan, Zi-Ling Yuan, Jia-Lin Zhao, Ying Huang, Zhang-Li Hu, Bin Jia

“…Based on the native terpenoids synthetic pathway, a dammarane-type ginsenosides synthetic pathway was established in <i>Chlamydomonas reinhardtii</i> by introducing <i>P. ginseng PgDDS</i>, CYP450 enzyme (<i>PgCYP716A47</i>), or/and <i>Arabidopsis thaliana</i> NADPH-cytochrome P450 reductase gene (<i>AtCPR</i>), which is responsible for producing dammarane-type ginsenosides. …”
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Transcriptome Analysis and Identification of the Cholesterol Side Chain Cleavage Enzyme BbgCYP11A1 From Bufo bufo gargarizans by Guangli Li, Tianyue An, Yu Li, Jinyang Yue, Ruoshi Huang, Jia Huang, Jincai Liang, Wei Yao, Liufang Huang, Yidu Chen, Rongrong Zhang, Aijia Ji, Lixin Duan

“…Interestingly, a plant NADPH-cytochrome P450 reductase (CPR) from Anemarrhena asphodeloides showed notably higher production than BbgAdx-2A-BbgAdR from B. bufo gargarizans. …”
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Production of a Novel Protopanaxatriol-Type Ginsenoside by Yeast Cell Factories by Chen Zhou, Ting Gong, Jingjing Chen, Tianjiao Chen, Jinling Yang, Ping Zhu

“…Then, we modified this engineered strain by replacing UGT109A1 with its mutant UGT109A1-K73A, overexpressing the cytochrome P450 reductase ATR2 from <i>Arabidopsis thaliana</i> and the key enzymes of UDP-glucose biosynthesis to increase the production of 3<i>β</i>,12<i>β</i>-Di-<i>O</i>-Glc-PPT, although these strategies did not show any positive effect on the yield of 3<i>β</i>,12<i>β</i>-Di-<i>O</i>-Glc-PPT. …”
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Toxicity, permeability and drug-metabolizing enzyme activities of curcumin analogues by Yakubu, Ndatsu

“…Furthermore, the effect of both compounds on drug-metabolizing enzyme activities, which were NADPH-cytochrome p450 reductase (CPR), UDP-glucuronosyltransferase (UGT), glutathione-S-transferase (GST) and sulfotransferase (SULT) in differentiated Caco-2 cells and adult male zebrafish were measured using colorimetric methods. …”
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Modular Engineering of <i>Saccharomyces cerevisiae</i> for De Novo Biosynthesis of Genistein by Yonghui Meng, Xue Liu, Lijuan Zhang, Guang-Rong Zhao

“…The downstream module was rebuilt to produce genistein from naringenin by pairing various isoflavone synthases and cytochrome P450 reductases. The optimal pair was used for the de novo biosynthesis of genistein with a titer of 31.02 mg/L from sucrose at 25 °C. …”
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Candidate Gene, <i>SmCPR1</i>, Encoding CPR1 Related to Plant Height of the Eggplant Dwarf Mutant <i>dwf</i> by Yang Lu, Shuangxia Luo, Na Li, Qiang Li, Wenchao Du, Weiwei Zhang, Ping Yu, Shuxin Xuan, Yanhua Wang, Jianjun Zhao, Xueping Chen, Shuxing Shen

“…A candidate gene <i>SmCPR1,</i> encoding cytochrome P450 reductases (CPR1), was identified by bulked segregant analysis (BSA). …”
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Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink by Damilare D. Akintade, Bhabatosh Chaudhuri

“…Cytochrome P450 reductase (CPR) abstracts electrons from Nicotinamide adenine dinucleotide phosphate H (NADPH), transferring them to an active Cytochrome P450 (CYP) site to provide a functional CYP. …”
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Challenges in the Heterologous Production of Furanocoumarins in <i>Escherichia coli</i> by Joana L. Rodrigues, Daniela Gomes, Lígia R. Rodrigues

“…Several strategies were tested to improve the enzymes solubility and activity with no success, including removing potential <i>N</i>-terminal transit peptides and expression of cytochrome P450 reductases, chaperones and/or enzymes to increase dimethylallylpyrophosphate availability. …”
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