Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7. by Hill, A, Worth, A, Elliott, T, Rowland-Jones, S, Brooks, J, Rickinson, A, McMichael, A

“…Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. …”

Two-dimensional crystallization conditions of human leukotriene C4 synthase requiring adjustment of a particularly large combination of specific parameters. by Zhao, G, Johnson, M, Schnell, JR, Kanaoka, Y, Haase, W, Irikura, D, Lam, B, Schmidt-Krey, I

“…To obtain a favorable diameter, salt, temperature, glycerol, and initial detergent concentration had to be controlled with great care. …”

Solubilization of receptors for the novel Ca2+-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate. by Berridge, G, Dickinson, G, Parrington, J, Galione, A, Patel, S

“…NAADP receptors could also be released into supernatant fractions upon detergent treatment of membranes prelabeled with [(32)P]NAADP. …”

A novel membrane based process to isolate recombinant human chemokine receptor CCR3 produced in Escherichia coli by Liu, J, Chen, C, Ge, B, Lu, J, Cui, Z

“…These conditions included solution pH, ionic strength, stirring speed, permeate flux, and detergent (Fos choline-14) concentration. Under optimized conditions, the MBP-CCR3 purity obtained in the retentate was about 96% and the recovery of MBP-CCR3 was close to 89% after ultrafiltration. …”

LipIDens: simulation assisted interpretation of lipid densities in cryo-EM structures of membrane proteins by Ansell, TB, Song, W, Coupland, CE, Carrique, L, Corey, RA, Duncan, AL, Cassidy, CK, Geurts, MMG, Rasmussen, T, Ward, AB, Siebold, C, Stansfeld, PJ, Sansom, MSP

“…Nevertheless, establishing the molecular identity of putative lipid and/or detergent densities remains challenging. Here we present LipIDens, a pipeline for molecular dynamics (MD) simulation-assisted interpretation of lipid and lipid-like densities in cryo-EM structures. …”

A lipid gating mechanism for the channel-forming O antigen ABC transporter by Caffalette, C, Corey, R, Sansom, M, Stansfeld, P, Zimmer, J

“…The channel’s periplasmic exit is sealed by detergent molecules that block solvent permeation. …”

Micelles protect membrane complexes from solution to vacuum. by Barrera, N, Di Bartolo, N, Booth, P, Robinson, C

“…Conversely, for membrane protein complexes in micelles, the transition into the gas phase usually leads to the disruption of interactions, particularly between cytoplasmic and membrane subunits, and a mass spectrum dominated by large aggregates of detergent molecules. We show that by applying nanoelectrospray to a micellar solution of a membrane protein complex, the heteromeric adenosine 5'-triphosphate (ATP)-binding cassette transporter BtuC2D2, we can maintain the complex intact in the gas phase of a mass spectrometer. …”

Subunit organization in the TatA complex of the twin arginine protein translocase: a site-directed EPR spin labeling study. by White, G, Schermann, S, Bradley, J, Roberts, A, Greene, N, Berks, B, Thomson, A

“…Nitroxide radicals were introduced at selected positions within the transmembrane helix of Escherichia coli TatA and used to probe the structure of detergent-solubilized TatA complexes by EPR spectroscopy. …”

A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes by Ishmukhametov, R, Russell, A, Berry, R

“…Fusogenic proteoliposomes, containing charged lipids and membrane proteins, fuse with oppositely charged bilayers, with no requirement for detergent or fusion-promoting proteins, and deliver large, fragile membrane protein complexes into the target bilayers. …”

Preparation And Characterization Of Poly(3-Hydroxybutyrate-Co-3- Hydroxyhexanoate) Reinforced Kenaf Fibre by Lee, Joyyi

“…Subsequently, bulk quantity ofP(3HB-co-3HHx) was recovered using solvent-free novel biological recovery method with yellow mealworms (Tenebrio molitor) and followed by further purification with water containing strong anionic detergent. P(3HB-co-3HHx) showed slightly better thermal stability than its solvent extracted derivative with the removal of inorganic compounds during the purification process.…”
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Synthesis of zeolite A in batch and semi-batch reactors by Mat, Ramli, Tavare, N. S.

“…One of these is in a detergent builder where it was suggested in 1970s to replace the phosphate builder for environmental reasons. …”
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Keratin-based bioplastic from chicken feathers by Gupta, Arun, Alashwal, B. Y., Bala, Md. Saad, Ramakrishnan, N.

“…Rad et al. reported a similar method, but for the removal of impurities, chicken feathers were washed with hot water mixed with detergent for 60 minutes, with the last step repeated twice. …”
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An eDrive-Based Estimation Method of the Laundry Unbalance and Laundry Inertia for Washing Machine Applications by Daniele Martinello, Sandro Rubino, Radu Bojoi

“…On the other hand, the laundry inertia’s estimation, performed at the beginning of the washing cycle, allows for the determination of the proper amounts of water and detergent, the water temperature, and the tumbling time. …”
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Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM by Valeria Kalienkova, Vanessa Clerico Mosina, Laura Bryner, Gert T Oostergetel, Raimund Dutzler, Cristina Paulino

“…Ca2+-bound and Ca2+-free conformations of nhTMEM16 in detergent and lipid nanodiscs illustrate the interactions with its environment and they reveal the conformational changes underlying its activation. …”
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The Influence of Iron Ions on Optical Brighteners and Their Application to Cotton Fabrics by Tihana Dekanić, Tanja Pušić, Ivo Soljačić, Branka Vojnović, Julija Volmajer Valh

“…Both groups of optical brighteners are intended for detergent formulations. The influence of iron ions was studied by absorption and fluorescence spectra in solution and by whiteness degree, identifying color differences using CIEL*a*b* coordinates and Ultraviolet Protection Factor (UPF) of cotton fabrics. …”
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Feed Quality and Feeding Level Effects on Faecal Composition in East African Cattle Farming Systems by Asep I. M. Ali, Shimels E. Wassie, Rainer Georg Joergensen, Daniel Korir, John P. Goopy, Klaus Butterbach-Bahl, Lutz Merbold, Uta Dickhoefer, Eva Schlecht

“…Across all data, the fungal C/bacterial C ratio was positively related to N and negatively to neutral detergent fiber concentrations in feces, indicating diet-induced shifts in the fecal microbial community.…”
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Evaluation of the relationship between color-tuning of photosynthetic excitons and thermodynamic stability of light-harvesting chromoproteins by M. RÄTSEP, A. LEHTMETS, L. KANGUR, K. TIMPMANN, K. LEIGER, Z.-Y. WANG-OTOMO, A. FREIBERG

“…While the pressure-induced decomposition product closely resembles the calcium-depleted form of the chromoproteins, the thermally induced products reveal monomeric B777 and dimeric B820 forms of bacteriochlorophyll a, similar to those seen in non-sulfur purple bacteria treated with detergent. The study further found nearly unison melting of the protein tertiary and secondary structures. …”
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Identification of a pre-active conformation of a pentameric channel receptor by Anaïs Menny, Solène N Lefebvre, Philipp AM Schmidpeter, Emmanuelle Drège, Zaineb Fourati, Marc Delarue, Stuart J Edelstein, Crina M Nimigean, Delphine Joseph, Pierre-Jean Corringer

“…These movements are highly similar in lipid vesicles and detergent micelles. These reorganizations are essentially completed within 2 ms and occur without channel opening at low proton concentration, indicating that they report a pre-active intermediate state in the transition pathway toward activation. …”
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Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle by Greg J Dodge, Alyssa J Anderson, Yi He, Weijing Liu, Rosa Viner, Barbara Imperiali

“…Here, we leverage cutting-edge detergent-free methods to stabilize, purify, and structurally characterize WbaP, a phosphoglycosyl transferase (PGT) from the Salmonella enterica (LT2) O-antigen biosynthesis. …”
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Study of Melamine-Formaldehyde/Phase Change Material Microcapsules for the Preparation of Polymer Films by Extrusion by Sara García-Viñuales, César Rubio, Lidia Martínez-Izquierdo, Beatriz Zornoza, Elena Piera, Miguel Ángel Caballero, Carlos Téllez

“…Under processing conditions, the microcapsules apparently preserved their properties, also maintaining their n-eicosane loading and heat storage capacity under washing conditions (water with detergent at 60 °C). The microcapsules synthesis has been scaled up for the fabrication of functional films by extrusion. …”
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