The site of attachment of retinal in bacteriorhodopsin. A resonance Raman study. by Rothschild, K, Argade, P, Earnest, T, Huang, K, London, E, Liao, M, Bayley, H, Khorana, H, Herzfeld, J

“…The fragments were recombined in different combinations into lipid/detergent mixtures and retinal was added to regenerate the chromophore. …”

Antigen presentation and the association of class-I molecules. by Elliott, T, Cerundolo, V, Ohlen, C, Ljunggren, H, Karre, K, Townsend, A

“…We have gone on to show that stable assembly of class-I molecules can be supported in detergent extracts of the mutant cells when specific peptides are added. …”

Characterization of the human sigma-1 receptor chaperone domain structure and binding immunoglobulin protein (BiP) interactions. by Ortega-Roldan, J, Ossa, F, Schnell, JR

“…We have developed methods for bacterial expression and reconstitution of the chaperone domain of human S1R into detergent micelles that enable its study by solution NMR spectroscopy. …”

Conformation and environment of channel-forming peptides: a simulation study. by Johnston, J, Cook, G, Tomich, J, Sansom, MS

“…This correlates with the ability of the W22 and R19 side chains to form H-bonds with the headgroups of lipid or detergent molecules. This study provides a first atomic resolution comparison of the structure and dynamics of NK4-M2GlyR-p22 peptides in membrane and membrane-mimetic environments, paralleling NMR and functional studies of these peptides.…”

New insights into the Tat protein transport cycle from characterising the assembled Tat translocon by Alcock, F, Berks, BC

“…The ephemeral nature of the Tat translocation site has so far precluded its isolation. We now report that detergent solubilization of membranes during active transport allows the recovery of receptor complexes that are associated with elevated levels of TatA. …”

Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs he... by Walker, B, Krishnasastry, M, Zorn, L, Kasianowicz, J, Bayley, H

“…Convenient new assays utilizing the 35S-labeled polypeptide to measure erythrocyte binding, oligomer formation in detergent and on target cells, and hemolysis have been developed. …”

One membrane protein, two structures and six environments: a comparative molecular dynamics simulation study of the bacterial outer membrane protein PagP. by Cox, K, Sansom, MS

“…NMR) and in six different environments (detergent micelles formed by dodecyl phosphocholine and by octyl glucoside, vs. four species of phospholipid bilayer). …”

DNA is replicated at the nuclear cage. by McCready, S, Godwin, J, Mason, D, Brazell, I, Cook, P

“…Structures resembling nuclei are released when HeLa cells are lysed in a detergent and 2 M salt. These nucleoids, which lack any organized membrane structure, contain all the nuclear DNA packaged within a cage of RNA and protein. …”

A gentle method for preparing cyto- and nucleo-skeletons and associated chromatin. by Jackson, D, Yuan, J, Cook, P

“…Cells are then permeabilized by the non-ionic detergent Triton X-100 or antibody and complement in a buffer whose ionic composition mimics that of the cytoplasm. …”

Decellularization of aorta tissue using sonication treatment as potential scaffold for vascular tissue engineering by N. Syazwani, N. Syazwani, Azhim, A., Morimoto, Y., Furukawa, K. S., Ushida, T.

“…Aorta samples are decellularized by sonication treatment with various treatment times and application of sodiumdodecyl sulfate (SDS) detergent with and without saline. The relation between decellularization and dissolved oxygen concentration is investigated. …”

The impact of acoustic intensity on solution parameters and decellularization using sonication treatment by Azhim, Azran, Shafiq, Mohammad, Rasyada, Rahman, Furukawa, Katsuko, Ushida, Takashi

“…The study indicates positive correlation of DO concentration and pH of SDS detergent with acoustic intensity and negative correlation between conductivity and acoustic intensity. …”

Fast and Efficient Mechanosynthesis of Aldonamides by Aminolysis of Unprotected Sugar Lactones by Abed Bil, Bemba Abdellahi, Gwladys Pourceau, Anne Wadouachi

“…Sugar amides, such as aldonamides, are interesting, sugar-based molecules used in various fields, from detergency to medicine. Nevertheless, their valorization, especially as alternatives to petroleum-based substances, can be slowed down by their synthetic pathway, which is generally not in accordance with green chemistry principles, and is also not economically competitive. …”
Get full text

Interaction of Cytochrome C Oxidase with Steroid Hormones by Ilya P. Oleynikov, Natalia V. Azarkina, Tatiana V. Vygodina, Alexander A. Konstantinov

“…The plots of K_i vs [DM] are linear for both estradiol and testosterone which may indicate an 1:1 stoichiometry competition between the hormones and the detergent. Binding of estradiol, but not of testosterone, brings about spectral shift of the oxidized CcO consistent with an effect on heme <i>a₃</i>³⁺. …”
Get full text

Cryo-EM structures of the DCPIB-inhibited volume-regulated anion channel LRRC8A in lipid nanodiscs by David M Kern, SeCheol Oh, Richard K Hite, Stephen G Brohawn

“…Conformational and symmetry differences between LRRC8A structures determined in detergent micelles and lipid bilayers related to reorganization of intersubunit lipid binding sites demonstrate a critical role for the membrane in determining channel structure. …”
Get full text

TEKSTİL ENDÜSTRİSİ ATIKSUYUNDA HETEROJEN FENTON PROSESİ İÇİN KATALİZÖR OLARAK DEMİR İÇEREN METAL OKSİT KULLANIMI by Merve DURGUT, Şefika KAYA, Yeliz AŞÇI

“…Textile industry wastewater contains high amounts of non-biodegradable organic compounds, high concentrations of dyestuffs, salt, detergent and soap. Therefore, it is of great importance to remove organic pollutants in this wastewater. …”
Get full text

Lipozyme 435-Mediated Synthesis of Xylose Oleate in Methyl Ethyl Ketone by Maria Carolina Pereira Gonçalves, Jéssica Cristina Amaral, Roberto Fernandez-Lafuente, Ruy de Sousa Junior, Paulo Waldir Tardioli

“…The reaction product was purified and presented an emulsion capacity close to that of a commercial sugar ester detergent. Finally, the repeated use of Lipozyme 435 showed a reduction in the reaction yields (by 48 and 19% in the xylose and oleic acid conversions, respectively), after ten 12 h-cycles.…”
Get full text

Conformational Variability in Ground-State CFTR Lipoprotein Particle Cryo-EM Ensembles by Luba A. Aleksandrov, Adrei A. Aleksandrov, Timothy J. Jensen, Joshua D. Strauss, Jonathan F. Fay

“…Recent advancements in structural biological methods have led to more than 10 published CFTR structures, and, so far, all of these structures of CFTR, determined by cryo-EM, have been limited to detergent-purified protein preparations. To visualize CFTR in an environment that more closely represents its native membranous environment, we utilized two different lipoprotein particle encapsulation techniques: one in which the ion channel is first purified and then reconstituted using the membrane scaffolding protein Saposin A and another that uses the solubilizing polymer Sokalan CP9 (DIBMA) to extract CFTR directly from membranes. …”
Get full text

Analysis of nutritional components, fatty acid value and mycotoxin contents of stored corn in Heilongjiang granaries by WANG Wei-wei, SONG Ge, WANG Li, ZHOU Hang, DONG Zheng-lin, LIU Kuan-bo, WANG Yong-wei, SONG Dan, LI Ai-ke

“…Meanwhile, there were no obvious differences in nutrient composition (moisture, total energy, crude protein, crude fat, dietary fiber, phosphorus, neutral detergent fiber, etc.), fatty acid value, mycotoxins (aflatoxin B1、ochratoxin and zearalenone) and swine bionic digestible energy among the four groups of corn (P>0.05). …”
Get full text

A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins by Min-Kyung Sung, Tanya R Porras-Yakushi, Justin M Reitsma, Ferdinand M Huber, Michael J Sweredoski, André Hoelz, Sonja Hess, Raymond J Deshaies

“…Overproduced yeast ribosomal protein (RP) Rpl26 fails to assemble into ribosomes and is degraded in the nucleus/nucleolus by a ubiquitin-proteasome system quality control pathway comprising the E2 enzymes Ubc4/Ubc5 and the ubiquitin ligase Tom1. tom1 cells show reduced ubiquitination of multiple RPs, exceptional accumulation of detergent-insoluble proteins including multiple RPs, and hypersensitivity to imbalances in production of RPs and rRNA, indicative of a profound perturbation to proteostasis. …”
Get full text

Protein and carbohydrate profiles of 'Massai' grass silage with pelleted citrus pulp and microbial inoculant by Amanda Nunes Assis dos Anjos, João Carlos de Carvalho Almeida, Carlos Renato Viegas, Pedro Henrique Ferreira da Silva, Leonardo Fiusa de Morais, Delci de Deus Nepomuceno, Carlos Augusto Brandão de Carvalho, Felipe Almeida Soares

“…Under increasing citrus pulp levels, there was a reduction in neutral detergent fiber and an increase in nonfibrous carbohydrate contents and in protein linked to fiber. …”
Get full text