OMV-based vaccine formulations against Shiga toxin producing Escherichia coli strains are both protective in mice and immunogenic in calves by Matias Fingermann, Lucía Avila, Maria Belén De Marco, Luciana Vázquez, Darío Nicolás Di Biase, Andrea Verónica Müller, Mirta Lescano, José Christian Dokmetjian, Sonsire Fernández Castillo, José Luis Pérez Quiñoy

“…Outer Membrane Vesicles (OMV) obtained after detergent treatment of gram-negative bacteria have been used over the past decades for producing many licensed vaccines. …”
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Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis by Satoshi Yamashita, Haruhiko Yamaguchi, Toshiyuki Waki, Yuichi Aoki, Makie Mizuno, Fumihiro Yanbe, Tomoki Ishii, Ayuta Funaki, Yuzuru Tozawa, Yukino Miyagi-Inoue, Kazuhisa Fushihara, Toru Nakayama, Seiji Takahashi

“…We show that HRT1, a cPT from Heveabrasiliensis, exhibits distinct RTase activity in vitro only when it is introduced on detergent-washed HeveaRPs (WRPs) by a cell-free translation-coupled system. …”
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Structural basis of lipid-droplet localization of 17-beta-hydroxysteroid dehydrogenase 13 by Shenping Liu, Ruth F. Sommese, Nicole L. Nedoma, Lucy Mae Stevens, Jason K. Dutra, Liying Zhang, David J. Edmonds, Yang Wang, Michelle Garnsey, Michelle F. Clasquin

“…Here we report crystal structures of full length HSD17B13 in complex with its NAD+ cofactor, and with lipid/detergent molecules and small molecule inhibitors from two distinct series in the ligand binding pocket. …”
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The Nanostructure of Alkyl-Sulfonate Ionic Liquids: Two 1-Alkyl-3-methylimidazolium Alkyl-Sulfonate Homologous Series by Hugo Marques, José Nuno Canongia Lopes, Adilson Alves de Freitas, Karina Shimizu

“…The functionalization of polymers with sulfonate groups has many important uses, ranging from biomedical applications to detergency properties used in oil-recovery processes. …”
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<i>Barbeya oleoides</i> Leaves Extracts: In Vitro Carbohydrate Digestive Enzymes Inhibition and Phytochemical Characterization by Alaa A. Khojah, Guillermo F. Padilla-González, Ammar Bader, Monique J. S. Simmonds, Michael Munday, Michael Heinrich

“…This study investigated the in vitro inhibitory potential of different solvent extracts of leaves of <i>Barbeya oleoides</i> on key enzymes related to type 2 diabetes mellitus (α-glucosidase and α-amylase) in combination with an aggregation assay (using 0.01% Triton X-100 detergent) to assess the specificity of action. The methanol extract was the most active in inhibiting α-glucosidase and α-amylase, with IC₅₀ values of 6.67 ± 0.30 and 25.62 ± 4.12 µg/mL, respectively. …”
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Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology by Erik Henrich, Oliver Peetz, Christopher Hein, Aisha Laguerre, Beate Hoffmann, Jan Hoffmann, Volker Dötsch, Frank Bernhard, Nina Morgner

“…We further indicate a lipid-dependent dimer formation of MraY translocase correlating with the enzymatic activity. The detergent-free synthesis of membrane protein/nanodisc samples and the analysis by LILBID mass spectrometry provide a versatile platform for the analysis of membrane proteins in a native environment.…”
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Pemanfaatan Limbah Delignifikasi Bioethanol dari Tandan Kosong Kelapa Sawit Menjadi Surfaktan Natrium Lignosulfonat by Widia Rini Hartari, Bigi Undadraja, Febrina Delvitasari, Maryanti, Ersan, Sarono

“… Surfaktan (Surface active agent) merupakan senyawa yang bersifat ampipilik (hidropilik dan hidropobik dalam satu molekul), sehingga dapat diaplikasikan sebagai defoaming, emulsifier, detergency, emuliency, dan lainnya. Tujuan penelitian ini adalah mendapatkan Surfaktan Natrium Lignosulfonat dari limbah proses delignifikasi bioethanol. …”
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Yield and biomass quality of the whole plant of four maize hybrids for silage production by Terzić Dušanka, Radosavljević Milica, Milašinović-Šeremešić Marija, Jovanović Života, Nikolić Valentina

“…The highest average digestibility of dry matter (61.00%) and NDF (NDFD - Neutral Detergent Fibres Digestibility) (26.20%) of the whole maize plant was determined in the ZP 707 hybrid, which also had the lowest average content of all lignocellulosic fibres.…”
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DNA-nanostructure-templated assembly of planar and curved lipid-bilayer membranes by Mostafa A. Elbahnasawy, Mahmoud L. Nasr

“…Lipid-bilayer nanodiscs and liposomes have been developed to stabilize membrane proteins in order to study their structures and functions. Nanodiscs are detergent-free, water-soluble, and size-controlled planar phospholipid-bilayer platforms. …”
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Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae by Xinbo Wang, Shanshan Li, Haicheng Wang, Wenqing Shui, Junjie Hu

“…Here, we isolated tubule-based microsomes from Saccharomyces cerevisiae via classical cell fractionation and detergent-free immunoprecipitation of Flag-tagged Yop1p, which specifically localizes to ER tubules. …”
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<b>Feeding behavior of dairy cows in feedlot and fed on crude glycerin levels in the diet by Murilo de Almeida Meneses, Fabiano Ferreira da Silva, Alex Resende Schio, Robério Rodrigues Silva, Dicastro Dias de Souza, Antônio Ferraz Porto Junior

“…There was a linear effect on consumption of dry matter and neutral detergent fiber (NDF) corrected for ash and protein (NDF); a similar effect was registered for intake and ruminating efficiencies of dry matter and NDF with the inclusion of crude glycerin in the diet (p < 0.05). …”
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The Aβ42 Peptide and IAPP Physically Interact in a Yeast-Based Assay by Daniel V. Kachkin, Veronika V. Lashkul, Natalia A. Gorsheneva, Sergey A. Fedotov, Maria S. Rubel, Yury O. Chernoff, Aleksandr A. Rubel

“…We demonstrated that fluorescently tagged IAPP forms detergent-resistant aggregates in yeast cells. Using the FRET approach, we showed that IAPP and Aβ aggregates co-localize and physically interact in yeast cells. …”
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Nuclear-import receptors as gatekeepers of pathological phase transitions in ALS/FTD by Bilal Khalil, Miriam Linsenmeier, Courtney L. Smith, James Shorter, Wilfried Rossoll

“…The common accumulation of TAR DNA-binding protein-43 (TDP-43), fused in sarcoma (FUS), and other nuclear RBPs in detergent-insoluble aggregates in the cytoplasm of degenerating neurons in ALS/FTD is connected to nuclear pore dysfunction and other defects in the nucleocytoplasmic transport machinery. …”
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Alkaline and Halophilic Protease Production by Bacillus luteus H11 and Its Potential Industrial Applications by Agnieszka Kalwasińska, Urszula Jankiewicz, Tamás Felföldi, Aleksandra Burkowska-But, Maria Swiontek Brzezinska

“…These properties make the protease suitable for applications in food, detergent and pharmaceutical industries, and also in environmental bioremediation.…”
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A Liquid Chromatography-Mass Spectrometry Method to Study the Interaction between Membrane Proteins and Low-Molecular-Weight Compound Mixtures by Hideo Ogiso, Ryoji Suno, Takuya Kobayashi, Masashi Kawami, Mikihisa Takano, Masaru Ogasawara

“…In this study, we succeeded in detecting the interaction of low-molecular-weight (LMW) compounds with a membrane protein mixture derived from cultured cells expressing target membrane proteins by using the size exclusion chromatography-mass spectrometry (SEC-MS) method under the condition of 0.001% lauryl maltose neopentyl glycol as detergent and atmospheric pressure chemical ionization. …”
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Cryo-EM structure of the Mycobacterium abscessus F1-ATPase by Wong, Chui Fann, Leow, Chen Yen, Grüber, Gerhard

“…No effect was observed in the presence of the detergent lauryldimethylamine oxide.…”
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Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein by Li, Yan, Jain, Neeraj, Limpanawat, Suweeraya, To, Janet, Quistgaard, Esben M., Nordlund, Par, Thanabalu, Thirumaran, Torres, Jaume

“…Titration of purified C-terminal endodomain of BAP31 against isotopically labeled SH protein in detergent micelles suggests direct interaction between the two proteins. …”
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Hand properties of woven fabric treated with commercial softeners / Nor Kamalia Othman Adli... [et al.] by Othman Adli, Nor Kamalia, Mat Fuad, Nur Afiqah, Mohd Rahim, Nik Nur Raihani, Zainal, Halimatul Saadiyah, Nasir, Eryna, Jaafar, Zuraida, Abdul Ghani, Suzaini

“…Three different brands of softener; Brand A, Brand B and Brand C were used, and the fabric samples were washed by using top load home washing machine for 48 minutes in each cycle with the detergent and softener added into the washing machine dispenser drawer following the instruction label on the softener’s bottle. …”
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Legume/Maize Intercropping and N Application for Improved Yield, Quality, Water and N Utilization for Forage Production by Haixing Zhang, Wei Shi, Shahzad Ali, Shenghua Chang, Qianmin Jia, Fujiang Hou

“…Compared with N1, the N3 and N4 treatments significantly increased fresh and hay yield, crude protein yield, crude protein concentration, and crude fat concentration of maize, legumes, and the whole silage system, and decreased the concentration of neutral detergent fiber (NDF) and acid detergent fiber (ADF). …”
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Ruminal silage degradability and productivity of forage and grain-type sorghum cultivars Degradabilidade ruminal das silagens e produtividade de cultivares de sorgo de tipo forrage... by José Antônio Resende, Marcos Neves Pereira, Renzo Garcia Von Pinho, Abeilard Henrique Fonseca, Aloísio Ricardo Pereira da Silva

“…Dry matter yield was 13.0 &plusmn; 2.3 (mean &plusmn; S.D.) t ha-1. The neutral detergent fiber content was 50.3 &plusmn; 3.8 and the DEF was 48.8 &plusmn; 2.8% (dry matter basis). …”
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