A robust mass spectrometry method for rapid profiling of erythrocyte ghost membrane proteomes by Fye, H, Mrosso, P, Bruce, L, Thezenas, M, Davis, S, Fischer, R, Rwegasira, G, Makani, J, Kessler, B

“…</p> <h4>Results</h4> <p>The results obtained demonstrate a robust method for membrane enrichment enabling consistent MS based characterisation of &gt; 900 RBC membrane proteins in single LC–MS/MS analyses. Non-detergent based membrane solubilisation methods using the tissue and supernatant fractions of isolated ghost membranes are shown to offer effective haemoglobin removal as well as diverse recovery including erythrocyte membrane proteins of high and low abundance.…”

The Vaccinia Virus A36R Protein Is a Type Ib Membrane Protein Present on Intracellular but Not Extracellular Enveloped Virus Particles by Van Eijl, H, Hollinshead, M, Smith, G

“…However, the polypeptide synthesized in the presence of microsomes was associated integrally with the membrane and was sensitive to digestion by exogenous protease without permeabilization of the membrane with detergent, indicating that the majority of the protein is exposed on the outside of the vesicle. …”

Investigation into sub-cellular CD4 distribution in human embryonic stem cell derived macrophages and its role in HIV-1 infection by van Wilgenburg, B

“…Previous pharmacological studies provide one line of evidence for an endosomal entry route which is dependent on Detergent Resistant Membranes (DRMs). However, these findings need confirmation using a genetic approach, as small molecules may have multiple non-specific effects. …”

Characterization of a novel, stage-specific, invariant surface protein in Trypanosoma brucei containing an internal, serine-rich, repetitive motif. by Nolan, D, Jackson, D, Windle, H, Pays, A, Geuskens, M, Michel, A, Voorheis, H, Pays, E

“…This integral membrane glycoprotein was purified by a combination of detergent extraction, lectin-affinity, and ion-exchange chromatography followed by preparative SDS-polyacrylamide gel electrophoresis. …”

TRIC-B channels display labile gating: evidence from the TRIC-A knockout mouse model. by Venturi, E, Matyjaszkiewicz, A, Pitt, S, Tsaneva-Atanasova, K, Nishi, M, Yamazaki, D, Takeshima, H, Sitsapesan, R

“…We therefore incorporated skeletal muscle SR vesicles derived from Tric-a-knockout mice into bilayers in order to characterise the biophysical properties of native TRIC-B without possible misclassification of the channels as TRIC-A, and without potential distortion of functional properties by detergent purification protocols. The native TRIC-B channels were ideally selective for cations. …”

Use of fluorescently labelled deoxyribonuclease I to spatially measure G-actin levels in migrating and non-migrating cells. by Cramer, L, Briggs, L, Dawe, H

“…Biochem. 95:377-385], when cells were fixed in formaldehyde and permeabilized in detergent, fluorescently-labelled DNase I specifically stained G-actin and not F-actin. 92-98% of actin molecules were stably retained in cells during fixation and permeabilization. …”

Membrane Interactions of α-Synuclein revealed by multiscale molecular dynamics simulations, Markov state models, and NMR by Amos, S-BTA, Schwartz, TC, Shi, J, Cossins, BP, Baker, TS, Taylor, RJ, Konrat, R, Sansom, M

“…Coarse-grained simulations of α-synuclein starting from the structure of αS when bound to a detergent micelle reveal the overall pattern of protein contacts to anionic lipid bilayers, whilst subsequent all-atom simulations provide details of conformational changes upon membrane binding. …”

Nutritive Value And Bioavailability Of Protein Feedstuffs For Broiler Chickens by Lim, Fairda

“…In Experiment 1 , the nutritive values of these feedstuffs were subjected to the following chemical analyses: proximate, minerals, detergent fibre, amino acids and in vitro digestibility . …”
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Isolation and characterization of an SDS-degrading Klebsiella oxytoca. by Abd. Shukor, Mohd. Yunus, Hussin, W. S., Rahman, M. F., Shamaan, Nor Aripin, Syed, Mohd. Arif

“…Sodium dodecyl sulfate (SDS) is one of the main components in the detergent and cosmetic industries. Its bioremediation by suitable microorganism has begun to receive greater attention as the amount of SDS usage increases to a point where treatment plants would not be able to cope with the increasing amount of SDS in wastewater. …”
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Quality of Asystasia leaves and stems 60 days after seeding at different shade levels. by Noordin Mokhtar, Suparjo, R. A., Halim, S., Jalaludin, C., Abu Bakar, Othman, Ahmad Shokri

“…Conversely, nutritive quality of stems decreased with shade : stem IVDDM concentrations were 766, 737, 693 and 694 g kg-1 DM while acid-detergent fiber (ADF) concentrations in stems were 283, 293, 345 and 371 g kg-1 DM. …”
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Effects of supplementation of rumen protected fats on rumen ecology and digestibility of nutrients in sheep by Behan, Atique Ahmed, Loh, Teck Chwen, Fakurazi, Sharida, Kaka, Ubedullah, Kaka, Asmatullah, Samsudin, Anjas Asmara @ Ab Hadi

“…The intake and digestibilities of dry matter, organic matter, crude protein and neutral detergent fibre were not affected, while those for ether extract and crude fibre differed (p < 0.05). …”
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A novel aqueous two phase system composed of surfactant and xylitol for purification of lipase from pumpkin (Cucurbita moschata) seed and recycling phase components by Amid, Mehrnoush, Hussin, Muhaini, Abd. Manap, Mohd. Yazid, Mustafa, Shuhaimi

“…Lipase is one of the important enzymes used in various industries such as food, detergent, pharmaceutical, textile, pulp and paper. …”
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Yield and quality of two kenaf varieties as affected by harvesting age by Mohammad Yusoff, Masnira, Abdul Halim, Ridzwan, Yusop, M. Rafii, Saad, Mohd Jani, Mohammad Yusoff, Martini

“…The crude protein (CP) of MHC 123 decreased slowly from 18.06 to 17.22% while CP of V 36 declined rapidly from 21.72 to 11.32% between 8 and 12 WAP. Acid detergent fibre content in MHC 123 increased slowly (31.72 to 36.86%) compared to V 36 (39.57 to 55.59%) from 8 to 12 WAP. …”
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Physicochemical characterization of lactose-based ester as potential pharmaceutical biosurfactant by Zaidan, Uswatun Hasanah, Abdul Rahman, Mohd Basyaruddin, Salleh, Abu Bakar, Basri, Mahiran, Othman, Siti Salhah

“…Furthermore, this non-ionic biosurfactant (yellowish) was found to behave like a water-soluble surfactant and an O/W emulsifier which potentially used for food, pharmaceuticals and detergent industries.…”
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Improving the nutritive value of rice bran by fungal and extrusion treatments by Lim, Yi Chin

“…The responses measured for fermented rice bran and extruded rice bran were 4.26 mg/g and 2.31 mg/g for soluble protein (SP), 11.29 mg/g and 3.97 mg/g for reducing sugar (RS), 10.9 % and 6.7 % for crude fibre (CF), 31.5 % and 21.1 % for neutral detergent fibre (NDF) respectively. The SP, RS, CF and NDF values for untreated rice bran were 2.24 mg/g, 6.35 mg/g, 7.3 % and 27.9 % respectively. …”
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Isolation of alkaliphilic bacteria producing highly alkaline thermostable lipase by Mad Zain, Azian Ezeila

“…Lipase is industrially important and commercially used in industrial such as detergent, food, leather and bioremediation. The aim of this thesis is to screen and isolate bacteria producing highly alkaline and thermostable lipase from hot springs in Malaysia. …”
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Structure prediction of a thermostable SR74 α-Amylase from Geobacillus stearothermophilus expressed in CTG-clade yeast Meyerozyma guilliermondii strain SO by Lim, Si Jie, Muhd Noor, Noor Dina, Salleh, Abu Bakar, Oslan, Siti Nurbaya

“…A thermostable SR74 α-amylase from Geobacillus stearothermophilus was found to have a huge potential in detergent industries due to its thermostability properties. …”

Penilaian Sifat-Sifat Fizikal, Nutrisi Dan Organoleptik Roti Dari Tepung Komposit by Ibrahim, Muhammad

“…Proximate composition, protein quality [total protein, digested and undigested protein, protein digestibility (in vitro), amino acids profile, protein efficiency ratio (PER), net protein ratio (NPR)] and fibre quality [total dietary fibre, soluble and insoluble dietary fibre, neutral detergent fibre, cellulose, hemicellulose, lignin] and anti nutritive components were determined in all samples. …”
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Relationship Of Tribological Property Of Different Flooring Textures On Human Mobility by Ooi, Yi Jing

“…The tribological property of these materials were being measured using pin on disc tribometer, under dry sliding and wet sliding with water, detergent solution and oil. The dynamic coefficient of friction, static coefficient of friction and specific wear rate of those flooring textures had been determined. …”
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Cloning and expression of pullulanase gene from locally isolated bacillus SP by Nik Mahmood, Nik Azmi, Md. Illias, Rosli, Md. Salleh, Madihah, Hassan, Osman, Kamaruddin, Kamarulzaman, Shahab, Neelam

“…As such, many pullulana e enzyme has been isolated and one has been showing optimum pH of 10-10.5 which is suitable for use in dishwasher detergent additive in removal of star ch stain. …”
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