Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis by Mst Nahid Akhter, Emilio Satoshi Hara, Koichi Kadoya, Masahiro Okada, Takuya Matsumoto

“…The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. …”
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Replacing Forage by Crude Olive Cake in a Dairy Sheep Diet: Effects on Ruminal Fermentation and Microbial Populations in Rusitec Fermenters by Jairo García-Rodríguez, Iván Mateos, Cristina Saro, Jesús S. González, María Dolores Carro, María José Ranilla

“…Experimental diets had a 50:50 forage-to-concentrate ratio and were formulated to contain the same protein (16.0%) and neutral detergent fiber (32.5%) levels. Compared with control fermenters, those fed the COC diet showed greater (<i>p ≤</i> 0.02) pH (6.07 vs. 6.22), diet disappearance (0.709 vs. 0.748), and butyrate proportions (18.0 vs. 19.4), but there were no differences in volatile fatty acids and ammonia production. …”
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Feeding behavior of Nellore heifers grazing during the dry season of the year supplemented with mineral salt and urea types by Aléx Schio Resende, Fabiano Ferreira da Silva, Evely Giovanna Leite Costa, Robério Rodrigues Silva, Dicastro Dias de Souza, Antonio Ferraz Porto Junior, Bismarck Moreira Santiago, Eli Santana de Oliveira Rodrigues, Edvaldo Nascimento Costa, Jemima Fialho Muricy

“…The dry matter intake, neutral detergent fiber and total digestible nutrients presented a similar effect, with higher values for heifers consuming supplements containing regular urea and slow urea compared to those of mixed urea. …”
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Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits by Xiao-Haitzi Daniel Puón-Peláez, Neil Ross McEwan, Roberto Carlos Álvarez-Martínez, Gerardo Mariscal-Landín, Gerardo Manuel Nava-Morales, Juan Mosqueda, Andrea Margarita Olvera-Ramírez

“…Rabbits that had either recovered from epizootic rabbit enteropathy or ones that had never had epizootic rabbit enteropathy were fed on a diet of 32% or 36% neutral detergent fiber until they were 70 days of age. At this point, the short-chain fatty acid and ammonia levels were measured in caecotroph and fecal samples and compared using 2 × 2 ANOVA. …”
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The Relative Importance of Herbage Nutritive Value and Climate in Determining Daily Performance per Cow in a Pasture-Based Dairy Farm by Federico Duranovich, Nicola Shadbolt, Ina Draganova, Nicolas López-Villalobos, Ian Yule, Stephen Morris

“…Herbage ME was of high relative importance in determining milk urea and body condition score, while neutral detergent fiber was a key driver of milk urea and liveweight (0.12 ≤ R² ≤ 0.16). …”
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Influence of light and color of advertising photography on consumers’ purchase intention by Fenglei Chen, Khunanan Sukpasjaroen, Thitinan Chankoson

“…The data were collected using a questionnaire focusing on consumers who purchased detergent products in major supermarkets in East China. …”
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Fermentation dynamics, nutritional quality, and heating capacity of mixed silages of elephant grass (Pennisetum purpureum Schum) and Leucaena (Leucaena leucocephala) by Janiele Santos de Araújo, Cleyton de Almeida Araújo, Amélia de Macedo, Crislane de Souza Silva, Judicael Janderson da Silva Novaes, Deneson de Oliveira Lima, Elizângela Nunes Borges, Glayciane Costa Gois, Gherman Garcia Leal de Araújo, Fleming Sena Campos

“…The increase in the inclusion of Leucaena in the composition of silages reduced gas and effluent losses, neutral and acid detergent fiber, cellulose, lignin, total and fiber carbohydrates, and total digestible nutrients, and resulted in increased dry matter, ether extract, and crude protein. …”
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Effect of exogenous fibrolytic enzymes cocktail incorporation on in vitro degradation and fermentation characteristics of oats straw based total mixed ration by YASIR AFZAL BEIGH, ABDUL MAJEED GANAI, HAIDAR ALI AHMAD, JAVID FAROOQ, GOWHER GULL SHEIKH, PARVAIZ AHMAD RESHI, ZULFAQARUL HAQ

“…There were significant improvements in degradability of dry matter, organic matter and neutral detergent fibre up to the enzyme dose of 0.60% DM (L3) with constant values thereafter. …”
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Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices by Joo-Yeun Oh, Jennifer Hamm, Xin Xu, Kristopher Genschmer, Ming Zhong, Jeffrey Lebensburger, Marisa B. Marques, Jeffrey D. Kerby, Jean-Francois Pittet, Amit Gaggar, Rakesh P. Patel

“…We show how incorporating other plasma factors that absorb light over the visible wavelength range (specifically bilirubin), coupled with truncating the wavelength range analyzed, or addition of mild detergent significantly improves fits allowing measurement of oxyHb, CFH and metHb with >90% accuracy. …”
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Effects of Ensiling <i>Oxytropis glabra</i> with Whole-Plant Corn at Different Proportions on Fermentation Quality, Alkaloid Swainsonine Content, and Lactic Acid Bacteria Populatio... by Ya Tao, Dongze Niu, Feng Li, Sasa Zuo, Qizhong Sun, Chuncheng Xu

“…As the proportion of corn in the silage increased, the pH, as well as the propionic acid, ammonia-N, dry matter, crude protein, and SW contents, decreased linearly, while the lactic acid, neutral detergent fiber, and residual water-soluble carbohydrate contents increased linearly. …”
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Ingestive behaviour in heifers fed diets with increasing levels of babassu cake by Kélvia Jácome De Castro, Iran Borges, Fabrícia Rocha Chaves Miotto, Silas Primola Gomes, Patrícia Guimarães Pimentel, Guilherme Rocha Moreira, Andrea Pereira Pinto, Jose Neuman Miranda Neiva

“…There was a linear increase in intake, and in feed and rumination efficiency for dry matter and neutral detergent fibre, with a reduction in total chewing time. …”
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Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration by Michal O. Nowicki, Josie L. Hayes, E. Antonio Chiocca, Sean E. Lawler

“…Western blotting showed that treatment with LiCl or small molecule GSK-3 inhibitors led to the rapid downregulation of detergent soluble vimentin levels across a panel of GBM-derived cells. …”
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Protein and Carbohydrate Fractionation to Evaluate Perennial Ryegrass (<i>Lolium perenne</i> L.) Accessions by Martin Gierus, Heba Sabry Attia Salama, Marc Lösche, Antje Herrmann, Friedhelm Taube

“…Although the variation was smaller for the protein fractions, the calculated usable protein content (uCP, sum of UDP and amount of synthesized microbial protein in the rumen) in the small intestine is mainly influenced by decreasing the neutral detergent fiber (NDF) and increasing the sugar content among cultivars. …”
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RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding by Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly, Frédéric Guérin, Sara Majello, Damien Ulveling, Anaïs Vayaboury, Nicolas Fernandez, Dilyana Dimitrova, Xavier Bussell, Yannick Fourne, Pierre Chaumat, Barbara André, Elodie Baldivia, Ulysse Godet, Mathieu Guinin, Vivien Moretto, Joy Ismail, Olivier Caille, Natacha Roblot, Carine Beaupère, Alexandrine Liboz, Ghislaine Guillemain, Bertrand Blondeau, Pierre Walrafen, Stuart Edelstein

“…Cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcoded beads for further processing with reverse transcription and preparation for cDNA sequencing. …”
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Evaluation of Near Infrared Spectroscopy (NIRS) and Remote Sensing (RS) for Estimating Pasture Quality in Mediterranean Montado Ecosystem by João Serrano, Shakib Shahidian, José Marques da Silva, Luís Paixão, Emanuel Carreira, Rafael Carmona-Cabezas, Julio Nogales-Bueno, Ana Elisa Rato

“…Laboratory reference methods for assessing quality parameters such as crude protein (CP) or fibers (neutral detergent fiber: NDF) require collection and analytical procedures involving technicians, time, and reagents, making them laborious and expensive. …”
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Development and Testing of a Low-Cost Inactivation Buffer That Allows for Direct SARS-CoV-2 Detection in Saliva by Brandon Bustos-Garcia, Sylvia Garza-Manero, Nallely Cano-Dominguez, Dulce Maria Lopez-Sanchez, Gonzalo Salgado-Montes de Oca, Alfonso Salgado-Aguayo, Felix Recillas-Targa, Santiago Avila-Rios, Victor Julian Valdes

“…Here, we optimized the composition of a buffer (PKTP), containing a protease, a detergent, and an RNase inhibitor, which is compatible with the RT-qPCR chemistry, allowing for direct SARS-CoV-2 detection from saliva without extracting RNA. …”
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Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium En... by Grace T. Ramena, Aarushi Sharma, Yan Chang, Zui Pan, Randolph C. Elble

“…Unexpectedly, cleavage caused a conformational shift: an N-terminal antibody that immunoprecipitated the precursor failed to precipitate the N-terminal product unless the product was first denatured with an ionic detergent. The E165Q mutation abolished the stimulation of SOCE caused by wildtype CLCA2, establishing that the metalloprotease activity is required for this regulatory function.…”
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Ammonia Volatilization, Forage Accumulation, and Nutritive Value of Marandu Palisade Grass Pastures in Different N Sources and Doses by Darlena Caroline da Cruz Corrêa, Abmael da Silva Cardoso, Mariane Rodrigues Ferreira, Débora Siniscalchi, Pedro Henrique de Almeida Gonçalves, Rodolfo Nussio Lumasini, Ricardo Andrade Reis, Ana Cláudia Ruggieri

“…The forage accumulation and chemical composition were evaluated in the third experiment; the response variables included forage accumulation, crude protein (CP), and neutral detergent fiber (NDF). Compared to urea, ammonium nitrate and ammonium sulfate reduced NH₃ losses by 84% and 87% and increased total forage accumulation by 14% and 23%, respectively. …”
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Strongyloidiasis Serological Analysis with Three Different Biological Probes and Their Electrochemical Responses in a Screen-Printed Gold Electrode by Francielli C. C. Melo, Luciano P. Rodrigues, Nágilla D. Feliciano, Julia M. Costa-Cruz, Vanessa S. Ribeiro, Bruna F. Matias-Colombo, Renata P. Alves-Balvedi, Luiz R. Goulart

“…We evaluated three different probes with single and multiple epitopes through electrochemical detection of specific IgG in serum for human strongyloidiasis diagnosis. (2) Methods: Screen-printed gold electrodes were used and probes consisting of two single-epitope synthetic peptides (D3 and C10) with different sequences, and a multi-epitope antigen [detergent phase (DP)—hydrophobic membrane proteins]. …”
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Upregulation of Sortilin, a Lysosomal Sorting Receptor, Corresponds with Reduced Bioavailability of Latent TGFβ in Mucolipidosis II Cells by Jarrod W. Barnes, Megan Aarnio-Peterson, Joy Norris, Mark Haskins, Heather Flanagan-Steet, Richard Steet

“…We found increased intracellular latent TGFβ1 complexes, caused by reduced secretion and stable localization in detergent-resistant lysosomes. Sortilin, a sorting receptor for hydrolases and TGFβ-related cytokines, was upregulated in ML-II fibroblasts as well as <i>GNPTAB</i>-null HeLa cells, suggesting a mechanism for inappropriate lysosomal targeting of TGFβ. …”
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