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3061
Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis
Published 2020-07-01“…The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. …”
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3062
Replacing Forage by Crude Olive Cake in a Dairy Sheep Diet: Effects on Ruminal Fermentation and Microbial Populations in Rusitec Fermenters
Published 2020-11-01“…Experimental diets had a 50:50 forage-to-concentrate ratio and were formulated to contain the same protein (16.0%) and neutral detergent fiber (32.5%) levels. Compared with control fermenters, those fed the COC diet showed greater (<i>p ≤</i> 0.02) pH (6.07 vs. 6.22), diet disappearance (0.709 vs. 0.748), and butyrate proportions (18.0 vs. 19.4), but there were no differences in volatile fatty acids and ammonia production. …”
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3063
Feeding behavior of Nellore heifers grazing during the dry season of the year supplemented with mineral salt and urea types
Published 2018-08-01“…The dry matter intake, neutral detergent fiber and total digestible nutrients presented a similar effect, with higher values for heifers consuming supplements containing regular urea and slow urea compared to those of mixed urea. …”
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3064
Effect of Feeding Insoluble Fiber on the Microbiota and Metabolites of the Caecum and Feces of Rabbits Recovering from Epizootic Rabbit Enteropathy Relative to Non-Infected Rabbits
Published 2022-05-01“…Rabbits that had either recovered from epizootic rabbit enteropathy or ones that had never had epizootic rabbit enteropathy were fed on a diet of 32% or 36% neutral detergent fiber until they were 70 days of age. At this point, the short-chain fatty acid and ammonia levels were measured in caecotroph and fecal samples and compared using 2 × 2 ANOVA. …”
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3065
The Relative Importance of Herbage Nutritive Value and Climate in Determining Daily Performance per Cow in a Pasture-Based Dairy Farm
Published 2021-05-01“…Herbage ME was of high relative importance in determining milk urea and body condition score, while neutral detergent fiber was a key driver of milk urea and liveweight (0.12 ≤ R<sup>2</sup> ≤ 0.16). …”
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3066
Influence of light and color of advertising photography on consumers’ purchase intention
Published 2022-12-01“…The data were collected using a questionnaire focusing on consumers who purchased detergent products in major supermarkets in East China. …”
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3067
Fermentation dynamics, nutritional quality, and heating capacity of mixed silages of elephant grass (Pennisetum purpureum Schum) and Leucaena (Leucaena leucocephala)
Published 2022-09-01“…The increase in the inclusion of Leucaena in the composition of silages reduced gas and effluent losses, neutral and acid detergent fiber, cellulose, lignin, total and fiber carbohydrates, and total digestible nutrients, and resulted in increased dry matter, ether extract, and crude protein. …”
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3068
Effect of exogenous fibrolytic enzymes cocktail incorporation on in vitro degradation and fermentation characteristics of oats straw based total mixed ration
Published 2023-10-01“…There were significant improvements in degradability of dry matter, organic matter and neutral detergent fibre up to the enzyme dose of 0.60% DM (L3) with constant values thereafter. …”
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3069
Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices
Published 2016-10-01“…We show how incorporating other plasma factors that absorb light over the visible wavelength range (specifically bilirubin), coupled with truncating the wavelength range analyzed, or addition of mild detergent significantly improves fits allowing measurement of oxyHb, CFH and metHb with >90% accuracy. …”
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3070
Effects of Ensiling <i>Oxytropis glabra</i> with Whole-Plant Corn at Different Proportions on Fermentation Quality, Alkaloid Swainsonine Content, and Lactic Acid Bacteria Populatio...
Published 2020-09-01“…As the proportion of corn in the silage increased, the pH, as well as the propionic acid, ammonia-N, dry matter, crude protein, and SW contents, decreased linearly, while the lactic acid, neutral detergent fiber, and residual water-soluble carbohydrate contents increased linearly. …”
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3071
Ingestive behaviour in heifers fed diets with increasing levels of babassu cake
Published 2024-03-01“…There was a linear increase in intake, and in feed and rumination efficiency for dry matter and neutral detergent fibre, with a reduction in total chewing time. …”
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3072
Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration
Published 2019-04-01“…Western blotting showed that treatment with LiCl or small molecule GSK-3 inhibitors led to the rapid downregulation of detergent soluble vimentin levels across a panel of GBM-derived cells. …”
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3073
Protein and Carbohydrate Fractionation to Evaluate Perennial Ryegrass (<i>Lolium perenne</i> L.) Accessions
Published 2024-01-01“…Although the variation was smaller for the protein fractions, the calculated usable protein content (uCP, sum of UDP and amount of synthesized microbial protein in the rumen) in the small intestine is mainly influenced by decreasing the neutral detergent fiber (NDF) and increasing the sugar content among cultivars. …”
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3074
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding
Published 2023-03-01“…Cell lysis is triggered by detergent diffusion, and RNA molecules are captured on the adjacent barcoded beads for further processing with reverse transcription and preparation for cDNA sequencing. …”
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3075
Evaluation of Near Infrared Spectroscopy (NIRS) and Remote Sensing (RS) for Estimating Pasture Quality in Mediterranean Montado Ecosystem
Published 2020-06-01“…Laboratory reference methods for assessing quality parameters such as crude protein (CP) or fibers (neutral detergent fiber: NDF) require collection and analytical procedures involving technicians, time, and reagents, making them laborious and expensive. …”
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3076
Development and Testing of a Low-Cost Inactivation Buffer That Allows for Direct SARS-CoV-2 Detection in Saliva
Published 2022-05-01“…Here, we optimized the composition of a buffer (PKTP), containing a protease, a detergent, and an RNase inhibitor, which is compatible with the RT-qPCR chemistry, allowing for direct SARS-CoV-2 detection from saliva without extracting RNA. …”
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3077
Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium En...
Published 2023-10-01“…Unexpectedly, cleavage caused a conformational shift: an N-terminal antibody that immunoprecipitated the precursor failed to precipitate the N-terminal product unless the product was first denatured with an ionic detergent. The E165Q mutation abolished the stimulation of SOCE caused by wildtype CLCA2, establishing that the metalloprotease activity is required for this regulatory function.…”
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3078
Ammonia Volatilization, Forage Accumulation, and Nutritive Value of Marandu Palisade Grass Pastures in Different N Sources and Doses
Published 2021-09-01“…The forage accumulation and chemical composition were evaluated in the third experiment; the response variables included forage accumulation, crude protein (CP), and neutral detergent fiber (NDF). Compared to urea, ammonium nitrate and ammonium sulfate reduced NH<sub>3</sub> losses by 84% and 87% and increased total forage accumulation by 14% and 23%, respectively. …”
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3079
Strongyloidiasis Serological Analysis with Three Different Biological Probes and Their Electrochemical Responses in a Screen-Printed Gold Electrode
Published 2021-03-01“…We evaluated three different probes with single and multiple epitopes through electrochemical detection of specific IgG in serum for human strongyloidiasis diagnosis. (2) Methods: Screen-printed gold electrodes were used and probes consisting of two single-epitope synthetic peptides (D3 and C10) with different sequences, and a multi-epitope antigen [detergent phase (DP)—hydrophobic membrane proteins]. …”
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3080
Upregulation of Sortilin, a Lysosomal Sorting Receptor, Corresponds with Reduced Bioavailability of Latent TGFβ in Mucolipidosis II Cells
Published 2020-04-01“…We found increased intracellular latent TGFβ1 complexes, caused by reduced secretion and stable localization in detergent-resistant lysosomes. Sortilin, a sorting receptor for hydrolases and TGFβ-related cytokines, was upregulated in ML-II fibroblasts as well as <i>GNPTAB</i>-null HeLa cells, suggesting a mechanism for inappropriate lysosomal targeting of TGFβ. …”
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