Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system by Maifiah, M.H. Mahamad, Jamal, Parveen, Nuge, Tamrin, Mohd Dali, Nor Soleha, Meor Hussin, Anis Shobirin, Farouk, Abd Alaziem, Mohd. Salleh, Hamzah

“…Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. …”
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Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris by Mohd Dali, Nor Soleha, Nuge, Tamrin, Mahamad Maifiah, Mohd Hafidz, Yusof, Faridah, Meor Hussin, Anis Shobirin, Farouk, Abd Alaziem, Mohd. Salleh, Hamzah

“…Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. …”
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Pengujian kadar iodium total dalam urin dengan metode destruksi basah dan destruksi kering by Perpustakaan UGM, i-lib

“…It was found that the optimum of NaOH amount, the optimum of 1% p-aminophenol and temperature (wet digestion) was 2 ml, 12 ml, and 60°C respectively. …”

THE ISOMERIZATION AND OXIDATION OF CAROTENOID COMPOUNDS IN THE OIL PALM FRUIT DURING PRODUCTIONS OF CPO Isomerisasi dan Oksidasi Senyawa Karotenold dalam Buah Kelapa Sawlt Selama... by Perpustakaan UGM, i-lib

“…The decrements have continued on the fruit digestion, pressing, and clarification processes. The isomerization is also indicatedby the decreasingof the main peak spectra absorptionintensity of a-and {karotene about :1:10,4%.The spectra showed hypsocromicshift by :1:3 nm. …”

Biogas from Palm Oil Mill Effluent (POME): recovery to electrical energy in Sawira Biogas Plant, Muadzam Shah, Pahang / Mohammad Zulfikri Ahat by Ahat, Mohammad Zulfikri

“…Biogas created from anaerobic digestion of POME can be used in gas engine to generate electric. …”
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Simultaneous inhibition of multiple steps in the processing of N-linked oligosaccharides does not impair immunoglobulin secretion from rat hybridoma cells by Hashim, Onn Haji, Cushley, W.

“…The structures of the N-linked oligosaccharides found on the Ig heavy chains isolated from treated cells or culture supernatants were shown to be qualitatively different from those associated with control Ig by persistent sensitivity to digestion by endo H. Furthermore, the electrophoretic mobilities of mu and gamma chains on SDS-PAGE derived from treated cells were consistently slower than those of control heavy chains. …”
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Minor modifications to the structure of tunicamycin lead to loss of the biological-activity of the antibiotic by Hashim, Onn Haji, Cushley, W.

“…In addition, the incorporation of tritiated sugars into acid-precipitable macromolecules was not inhibited. Endoglycosidase H digestion of isolated IgG molecules further suggested that the analogues employed did not interfere with qualitative glycosylation at the level of N-acetylglucosamine transferases I and II in the golgi apparatus. …”

Role of processing of N-linked oligosaccharides in control of immunoglobulin secretion from rat hybridomas by Hashim, Onn Haji, Cushley, W.

“…The inhibitor of glucosidase I, castanospermine, prevents conversion of the N-linked carbohydrate groups of IgM to a complex form as assessed by resistance to digestion by endo H. The rate of secretion of IgM or IgG from the treated cells is not diminished relative to controls. …”

Novel angiotensin I-converting enzyme inhibitory peptides derived from edible mushroom Agaricus bisporus (J.E. Lange) Imbach identified by LC-MS/MS by Lau, C.C., Abdullah, N., Shuib, A.S., Aminudin, N.

“…These peptides exhibited high ACE inhibitory activity after gastrointestinal digestion. Lineweaver-Burk plots suggested that AHEPVK and RIGLF act as competitive inhibitors against ACE, whereas PSSNK acts as a non-competitive inhibitor. …”

Current progress on removal of recalcitrance coloured particles from anaerobically treated effluent using coagulation–flocculation by Abu Zahrim Yaser

“…The palm oil industry is the most important agro industries in Malaysia and most of the mills adopt anaerobic digestion as their primary treatment for palm oil mill effluent (POME). …”
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Production of biogas from palm oil mill effluent by Ravindra Pogaku, Kai, Yieng Yong, V. Prasarnth Raaj Veera Rao

“…Malaysia with an average crude palm oil production of more than 13 million tons per year is estimated produce total of palm oil mill effluent (POME) of 53 million tons/year. Batch anaerobic digestion of 8l palm oil mill effluent was studied in a 25l bioreactor at 30°C (thermophilic condition) and pH controlled at 7. …”
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A review: resistant starch, a promising prebiotic for obesity and weight management by Thompson, Mayrilyn Solo, Tan, Hui Yan, Saari, Nazamid, Sarbini, Shahrul Razid

“…Therefore, it is reviewed in this article that RS as a soluble fiber with prebiotic potential for its ability to resist digestion and passes through the colon as well as positively stimulating the fermentation of gut microbiota. …”

Recovery of Nickel from spent hydrogenation catalyst using Chelating resin by Dzulkefly, K., Haron, M.J., Lim, Wen Huei, Woon, Chin Chan

“…Spent catalyst samples from hydrogenation of palm and palm kernel oils were subjected to soxhlet extraction to remove oil residue, digestion with concentrated sulfuric acid and nickel extraction using chelating ion exchange resin. …”
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Comparative study on the rumen ciliate populations in small experimental herds of water buffalo and Kedah Kelantan cattle in Malaysia by Imai, S., Abdullah, N., Ho, Y.W., Jalaludin, S., Hussain, H.Y., Onodera, R., Kudo, H.

“…This suggests that Malaysian cattle and water buffalo possess a ciliate protozoal composition favorable for digestion of cellulosic feed stuffs. There were no differences in the ciliate profile between the cattle and water buffalo. …”
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3-Hydroxy-3-methylglutaryl CoA reductase of Hevea latex: The occurrence of a heat-stable activator in the C-serum by Mohd. Isa, Ramli B., Sipat, A.B.

“…Although not essential, dithiothreitol complemented the activation effect of the boiled C-serum and the optimal concentration was 10 mM. Trypsin digestion of the boiled C-serum resulted in the complete loss of the activation effect. …”
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Phytase: application in food industry by Suhairin, Afinah, Abd. Manap, Mohd. Yazid, Meor Hussin, Anis Shobirin, Mustafa, Shuhaimi

“…The resulting complexes are insoluble, difficult for humans to hydrolyze during digestion, and thus, typically are nutritionally less available for absorption. …”
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A quick DNA extraction protocol: without liquid nitrogen in ambient temperature by Ferdous, Jannatul, Musa, Mohamed Hanafi, Yusop, Mohd Rafii, Syed Muhammad, Sharifah Kharidah

“…The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion.…”
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A chloroplast DNA (cpDNA) extraction protocol for diversity analysis of oil palm (Elaeis sp.) by Ho, Carl Miew, Ooi, Leslie C. L., Tan, S. G., Ho, Chai Ling, Singh, Rajinder

“…This paper describes a method for extracting enriched oil palm chloroplast DNA (cpDNA) done on six palms of different origins from Angola, Nigeria, Ghana, Madagascar and Suriname. Restriction enzyme digestion was used to evaluate the successful extraction of the oil palm cpDNA. …”
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Production of resistant starch type III from native sago starch as a potential prebiotic by Ahmad, Abdul Muhaimin, Mohd Zaman, Siti Aisyah, Sarbini, Shahrul Razid

“…Resistant starch (RS) is the sum of starch that escape upper gastrointestinal digestion and can be fermented by colonic microorganisms to produce beneficial metabolites such as short chain fatty acids (SCFA). …”
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Biological pretreatment of lignocellulosic biomass for volatile fatty acid production by Abd Aziz, Suraini, Ibrahim, Mohamad Faizal, Jenol, Mohd A.

“…Biomass-derived VFA is one of the potential alternatives for VFA production that required only a mild pretreatment process to open up the lignocellulosic biomass structure for anaerobic digestion (AD), whereas some of the biomass like food waste, manure, sludge, or any biodegradable biomass do not require any pretreatments. …”
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