Researching the Efficacy of Studio Education and the Profession’s Futurity by Michael Karassowitsch

“…Although it is limited by faculty knowledge and student expectation, we can conclude characteristic effects whereby this approach will lead to directed evolution of the educational environment and influence professional practice.…”
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Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity by Dmitry A. Gorbachev, Dmitry B. Staroverov, Konstantin A. Lukyanov, Karen S. Sarkisyan

“…Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.…”
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Enzyme engineering for functional lipids synthesis: recent advance and perspective by Ailin Guan, Yue Hou, Run Yang, Jiufu Qin

“…This article aims to review recent advances in protein engineering, encompassing approaches from directed evolution to rational design, with the goal of improving the properties of lipid-modifying enzymes. …”
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A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. by Sheng-Yi Wu, Yurong Wen, Nelson B C Serre, Cathrine Charlotte Heiede Laursen, Andrea Grostøl Dietz, Brian R Taylor, Mikhail Drobizhev, Rosana S Molina, Abhi Aggarwal, Vladimir Rancic, Michael Becker, Klaus Ballanyi, Kaspar Podgorski, Hajime Hirase, Maiken Nedergaard, Matyáš Fendrych, M Joanne Lemieux, Daniel F Eberl, Alan R Kay, Robert E Campbell, Yi Shen

“…Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. …”
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In-vitro engineering of novel bioactivity in the natural enzymes by Vishvanath Tiwari

“…Enzyme engineering technology involves directed evolution, rational designing, semi-rational designing and structure-based designing using chemical modifications. …”
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Rational and semi-rational engineering of cytochrome P450s for biotechnological applications by Lian-Hua Xu, Yi-Ling Du

“…For particular uses, enzyme properties of P450s can be further optimized through directed evolution, rational, and semi-rational engineering approaches, all of which introduce mutations within the P450 structures. …”
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Design and Evolution of Engineered Biological Systems by Kelly, Jason, Michener, Josh, Endy, Drew

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The structure of genotype-phenotype maps makes fitness landscapes navigable by Greenbury, SF, Louis, AA, Ahnert, SE

“…Our findings have broad implications for the prediction of natural evolutionary outcomes and for directed evolution.…”

Next Step in Gene Delivery: Modern Approaches and Further Perspectives of AAV Tropism Modification by Maxim A. Korneyenkov, Andrey A. Zamyatnin

“…In recent decades, several techniques have been developed to overcome this barrier, such as the rational design, directed evolution and chemical conjugation of targeting molecules with a capsid. …”
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Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast by Ballesteros Antonio, Maté Diana, García-Ruiz Eva, Martinez Angel T, Alcalde Miguel

“…</p> <p>Conclusions</p> <p>The existing tradeoff between activity and stability determined from many point mutations discovered by directed evolution and other protein engineering means can be circumvented combining different tools of <it>in vitro </it>evolution.…”
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Inceasing isopropanol tolerance in escherichia coli using randomized libraries of camp receptor protein by Lim, Therese Shi Min.

“…Here in this work, we used directed evolution method to mutate the global transcription factor, cAMP Receptor Protein (CRP), to engineer isopropanol tolerance in Escherichia Coli (E. …”
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Thermostability engineering of industrial enzymes through structure modification by Nezhad, Nima Ghahremani, Raja Abd Rahman, Raja Noor Zaliha, M. Normi, Yahaya, Oslan, Siti Nurbaya, Mohd Shariff, Fairolniza, Leow, Adam Thean Chor

“…Thermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus, enzyme engineering through directed evolution, semi-rational design and rational design are commonly employed to construct desired thermostable mutants. …”

Evolution of phage display libraries for therapeutic antibody discovery by Yang Zhang

“…Phage display technology, originally developed for peptide directed evolution, has been extensively applied to discovery of fully human antibodies due to its unprecedented advantages. …”
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Protein engineering of amine transaminases by Qinglong Meng, Carlos Ramírez-Palacios, Carlos Ramírez-Palacios, Hein J. Wijma, Dick B. Janssen

“…Various strategies to improve robustness of amine transaminases and to redirect their substrate specificity have been explored, including directed evolution, rational design and computation-supported engineering. …”
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Optimal enzyme utilization suggests that concentrations and thermodynamics determine binding mechanisms and enzyme saturations by Asli Sahin, Daniel R. Weilandt, Vassily Hatzimanikatis

“…It can further guide the directed evolution of enzymes and fill in the knowledge gaps in enzyme kinetics.…”
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Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae. by Chenlu Zhang, Ligia Acosta-Sampson, Vivian Yaci Yu, Jamie H D Cate

“…Efforts to use directed evolution to improve ST16 from a chromosomally-integrated copy were not successful, due to background growth of yeast on other carbon sources present in the selection medium. …”
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The Brain and the New Foundations of Mathematics by Alexey V. Melkikh

“…The consistency of mathematics is due to directed evolution, which results in effective structures. …”
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The Structure and Function of Paraoxonase-1 and Its Comparison to Paraoxonase-2 and -3 by Ajda Taler-Verčič, Marko Goličnik, Aljoša Bavec

“…However, possible substrates include homocysteine thiolactone, an analogue of natural quorum-sensing molecules, and the recently discovered derivatives of arachidonic acid—bioactive δ-lactones. Directed evolution, site-directed mutagenesis, and kinetic studies provide comprehensive insights into the active site and catalytic mechanism of PON1. …”
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Structural basis of the recognition of adeno-associated virus by the neurological system-related receptor carbonic anhydrase IV. by Ran Zhang, Yixiao Liu, Fengxi Yu, Guangxue Xu, Lili Li, Baobin Li, Zhiyong Lou

“…These findings provide new insights into the novel receptor recognition mechanism of AAV generated by directed evolution and highlight the application of the BBR method to studying the virus-receptor molecular mechanism.…”
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Target-switch SELEX: Screening with alternating targets to generate aptamers to conserved terminal dipeptides by Zachary William Cutts, Jessica M. Hong, Shirley Shao, Alexander Tran, Michelle Dimon, Marc Berndl, Diana Wu, Annalisa Pawlosky

“…Summary: Systematic evolution of ligands by exponential enrichment (SELEX) encompasses a wide variety of high-throughput screening techniques for producing nucleic acid binders to molecular targets through directed evolution. We describe here the design and selection steps for discovery of DNA aptamers with specificity for the two consecutive N-terminal amino acids (AAs) of a small peptide (8–10 amino acids). …”
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