Engineering of GH11 Xylanases for Optimal pH Shifting for Industrial Applications by In Jung Kim, Soo Rin Kim, Uwe T. Bornscheuer, Ki Hyun Nam

“…For the optimal pH shifting of GH11 xylanase in industrial applications, various protein engineering studies using directed evolution, rational engineering, and in silico approaches have been adopted. …”
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The right stuff; realizing the potential for enhanced biomass production in microalgae by Sowmya Subramanian, Richard T Sayre

“…In this review we assess some of the recent advances in algal strain selection, directed evolution, genetic engineering and molecular-assisted breeding that offer the potential for increased algal biomass production.…”
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Developing a cell-bound detection system for the screening of oxidase activity using the fluorescent peroxide sensor roGFP2-Orp1 by Herzog, PL, Borghi, E, Traxlmayr, MW, Obinger, C, Sikes, HD, Peterbauer, CK

“…Accurate yet efficient high-throughput screenings have emerged as essential technology for enzyme engineering via directed evolution. Modern high-throughput screening platforms for oxidoreductases are commonly assisted by technologies such as surface display and rely on emulsification techniques to facilitate single-cell analysis via fluorescence-activated cell sorting. …”
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Establishing an antibody mimetic applicable to targeting in the gastrointestinal tract by Wicke, NS

“…I also established phage display of SBTI to enable directed evolution. As proof of concept, gastrobody binders to domains of two toxins of the enteric pathogen Clostridium difficile were developed. …”

Enhanced secretory production of hemolysin-mediated cyclodextrin-glucanotransferase in Escherichia coli by random mutagenesis of the ABC transporter system. by Kheng, Oon Low, Mahadi, Nor Muhammad, Abdul Rahim, Raha, Rabu, Amir, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Illias, Rosli

“…To produce an improved-secretion variant, the hly components (hlyAs, hlyB and hlyD) were engineered by directed evolution using error-prone PCR. Hly mutants were screened on solid LB-starch plate for halo zone larger than the parent strain. …”
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Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B by Kun-Hwa Lee, Mark D. Distefano, Burckhard Seelig

“…Immobilized PLP is a valuable tool to isolate unknown PLP-dependent enzymes in nature or to perform in vitro selection or directed evolution on existing or de novo PLP-dependent enzymes. …”
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Evolutionary graph theory beyond pairwise interactions: Higher-order network motifs shape times to fixation in structured populations. by Yang Ping Kuo, Oana Carja

“…To design population topologies that can accelerate rates of solution discovery in directed evolution problems or for evolutionary optimization applications, we must first systematically understand how population structure shapes evolutionary outcome. …”
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2064 by Timothy P. Riley, Juan Mendoza, K. C. Garcia, Brian M. Baker

“…METHODS/STUDY POPULATION: To address this drawback to T-cell-based immunotherapies, we developed a novel protein engineering approach to define the peptide specificity of a given TCR. Here, directed evolution in a yeast display system produced a large scale peptide library, where recognition by the melanoma reactive DMF5 TCR acted as the guiding selective pressure. …”
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Current Understanding of Potential Linkages between Biocide Tolerance and Antibiotic Cross-Resistance by Kent Coombs, Cristina Rodriguez-Quijada, Jason O. Clevenger, Alexis F. Sauer-Budge

“…Questions still remain about whether the directed evolution of biocide tolerance and the associated antibiotic cross-resistance in a laboratory are or are not representative of real-world settings. …”
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SpSrtA-Catalyzed Isopeptide Ligation on Lysine Residues by Jiajia Wu, Tianyu Chu, Jian Hao, Liang Lin

“…We further developed a high-throughput yeast display screening method for the directed evolution of SpSrtA*, achieving a 10-fold improvement in the labeling efficiency of this surface protein. …”
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Facilitating Machine Learning‐Guided Protein Engineering with Smart Library Design and Massively Parallel Assays by Hoi Yee Chu, Alan S. L. Wong

“…Typically, exhaustive mutational screens or directed evolution experiments are used for the identification of the best design or for improvements to the wild‐type variant. …”
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Data-driven strategies for the computational design of enzyme thermal stability: trends, perspectives, and prospects by Dou Zhixin, Sun Yuqing, Jiang Xukai, Wu Xiuyun, Li Yingjie, Gong Bin, Wang Lushan

“…Although traditional methods such as directed evolution and classical rational design contribute greatly to this field, the enormous sequence space of proteins implies costly and arduous experiments. …”
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Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation by Danielle G. May, Kelsey L. Scott, Alexandre R. Campos, Kyle J. Roux

“…Recently, two new ligases termed TurboID and miniTurbo were developed using directed evolution of the BioID ligase and were able to produce robust biotinylation following a 10 min incubation with excess biotin. …”
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Programmable CRISPR‐Cas transcriptional activation in bacteria by Hsing‐I Ho, Jennifer R Fang, Jacky Cheung, Harris H Wang

“…Using this platform, we identified a novel CRISPR activator, dCas9‐AsiA, that could activate gene expression by more than 200‐fold across genomic and plasmid targets with diverse promoters after directed evolution. The evolved dCas9‐AsiA can simultaneously mediate activation and repression of bacterial regulons in E. coli. …”
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Development of a formaldehyde biosensor with application to synthetic methylotrophy by Woolston, Benjamin M, Roth, Timothy, Kohale, Ishwar, Liu, David R, Stephanopoulos, Gregory

“…This biosensor offers a quick and simple method for sensitively detecting formaldehyde, and has the potential to be used as the basis for directed evolution of Mdh and dynamic formaldehyde control strategies for establishing synthetic methylotrophy.…”
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Deep learning to design nuclear-targeting abiotic miniproteins by Schissel, Carly K, Mohapatra, Somesh, Wolfe, Justin M, Fadzen, Colin M, Bellovoda, Kamela, Wu, Chia-Ling, Wood, Jenna A, Malmberg, Annika B, Loas, Andrei, Gómez-Bombarelli, Rafael, Pentelute, Bradley L

“…We combined high-throughput experimentation with a directed evolution-inspired deep-learning approach in which the molecular structures of natural and unnatural residues are represented as topological fingerprints. …”
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Medical Engineering and Medical Physics: Metagenomic Sequencing for Viral Diagnostics and Discovery by Ye, Simon Huang

“…Metagenomic sequencing can also be utilized to perform large scale screening for the directed evolution of viral vectors. Adeno-associated virus (AAV) is a non-pathogenic virus that infects humans and commonly used as a vector for gene therapy. …”
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The evolutionary paths towards complexity: a metabolic perspective by Weng, Jing-Ke

“…Future studies, focused on fine sampling of metabolic enzymes and pathways in phylogenetically related plant species, or employing directed evolution strategies in synthetic systems, will significantly broaden our perspective on how biological complexity arises at the metabolic level.…”
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A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE by Yap, Wei Sheng, Thibault, Guillaume

“…The isolated high molecular weight, randomly assembled cDNA megaplasmid library may be useful for high-throughput directed evolution experiments and may be adapted for use in other model organisms.…”
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UniKP: a unified framework for the prediction of enzyme kinetic parameters by Han Yu, Huaxiang Deng, Jiahui He, Jay D. Keasling, Xiaozhou Luo

“…We have demonstrated the application of UniKP and EF-UniKP in several enzyme discovery and directed evolution tasks, leading to the identification of new enzymes and enzyme mutants with higher activity. …”
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