Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis by Hongguan Xing, Hongguan Xing, Pingping Wang, Xing Yan, Yi Yang, Xinliang Li, Rui Liu, Zhihua Zhou, Zhihua Zhou

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State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, an... by Milica Crnoglavac Popović, Marija Stanišić, Radivoje Prodanović

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Exploring the Comprehensive Kozak Sequence Landscape for AAV Production in Sf9 System by Oleksandr Kondratov, Sergei Zolotukhin

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Genome‐scale engineering for systems and synthetic biology by Kevin M Esvelt, Harris H Wang

Subjects: “…directed evolution…”
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Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues by Kelsi R Hall, Katherine J Robins, Elsie M Williams, Michelle H Rich, Mark J Calcott, Janine N Copp, Rory F Little, Ralf Schwörer, Gary B Evans, Wayne M Patrick, David F Ackerley

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Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture by Guanying Wang, Fuqiu Wang, Zhongping Xu, Ying Wang, Can Zhang, Yi Zhou, Fengjiao Hui, Xiyan Yang, Xinhui Nie, Xianlong Zhang, Shuangxia Jin

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Measuring the tolerance of the genetic code to altered codon size by Erika Alden DeBenedictis, Dieter Söll, Kevin M Esvelt

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Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase by Patrícia T. Borges, Diogo Silva, Tomás F.D. Silva, Vânia Brissos, Marina Cañellas, Maria Fátima Lucas, Laura Masgrau, Eduardo P. Melo, Miguel Machuqueiro, Carlos Frazão, Lígia O. Martins

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Directed Evolution of Seneca Valley Virus in Tumorsphere and Monolayer Cell Cultures of a Small-Cell Lung Cancer Model by Shakeel Waqqar, Kai Lee, Blair Lawley, Timothy Bilton, Miguel E. Quiñones-Mateu, Mihnea Bostina, Laura N. Burga

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COMPUTER-AIDED PROTEIN DIRECTED EVOLUTION: A REVIEW OF WEB SERVERS, DATABASES AND OTHER COMPUTATIONAL TOOLS FOR PROTEIN ENGINEERING by Rajni Verma, Ulrich Schwaneberg, Danilo Roccatano

Subjects: “…directed evolution…”
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Repurposing Glutathione Transferases: Directed Evolution Combined with Chemical Modification for the Creation of a Semisynthetic Enzyme with High Hydroperoxidase Activity by Irene Axarli, Farid Ataya, Nikolaos E. Labrou

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Protein Binder (ProBi) as a New Class of Structurally Robust Non-Antibody Protein Scaffold for Directed Evolution by Phuong Ngoc Pham, Maroš Huličiak, Lada Biedermannová, Jiří Černý, Tatsiana Charnavets, Gustavo Fuertes, Štěpán Herynek, Lucie Kolářová, Petr Kolenko, Jiří Pavlíček, Jiří Zahradník, Pavel Mikulecky, Bohdan Schneider

Subjects: “…directed evolution…”
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Structure-Guided Directed Evolution of Highly Selective P450-Based Magnetic Resonance Imaging Sensors for Dopamine and Serotonin by Brustad, Eric M., Lelyveld, Victor S., Snow, Christopher D., Crook, Nathan, Jung, Sang Taek, Martinez, Francisco M., Scholl, Timothy J., Arnold, Frances H., Jasanoff, Alan Pradip

“…Paramagnetic metalloproteins can be effective MRI sensors due to the selectivity imparted by the protein active site and the ability to tune protein properties using techniques such as directed evolution. Here, we show that structure-guided directed evolution of the active site of the cytochrome P450‐BM3 heme domain produces highly selective MRI probes with submicromolar affinities for small molecules. …”
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Novel chemical- and protein-mediated methods for glucosamine detection by Linshu Chen, Pedro Laborda, Zhipeng Cai, Andrew Kevin Hagan, Aimin Lu, Josef Voglmeir, Li Liu

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Directed evolution of a transcription factor PbrR to improve lead selectivity and reduce zinc interference through dual selection by Xiaoqiang Jia, Yubing Ma, Rongrong Bu, Tingting Zhao, Kang Wu

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Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing by David Rodríguez-Escribano, Rocío Pliego-Magán, Felipe de Salas, Pablo Aza, Patrizia Gentili, Petri Ihalainen, Thomas Levée, Valérie Meyer, Michel Petit-Conil, Sandra Tapin-Lingua, Michael Lecourt, Susana Camarero

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Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights by Estrella Lopez-Gordo, Kyle Chamberlain, Jalish Mahmud Riyad, Erik Kohlbrenner, Thomas Weber

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Increased efficiency of Campylobacter jejuni N-oligosaccharyltransferase PglB by structure-guided engineering by Julian Ihssen, Jürgen Haas, Michael Kowarik, Luzia Wiesli, Michael Wacker, Torsten Schwede, Linda Thöny-Meyer

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Efficient Oxidation of Methyl Glycolate to Methyl Glyoxylate Using a Fusion Enzyme of Glycolate Oxidase, Catalase and Hemoglobin by Xiangxian Ying, Can Wang, Shuai Shao, Qizhou Wang, Xueting Zhou, Yanbing Bai, Liang Chen, Chenze Lu, Man Zhao, Zhao Wang

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Resilience of small intestinal beneficial bacteria to the toxicity of soybean oil fatty acids by Sara C Di Rienzi, Juliet Jacobson, Elizabeth A Kennedy, Mary E Bell, Qiaojuan Shi, Jillian L Waters, Peter Lawrence, J Thomas Brenna, Robert A Britton, Jens Walter, Ruth E Ley

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