Cybernetics and Directed Evolution by Oleksandr Palagin

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Directed evolution in mammalian cells by Hendel, Samuel J, Shoulders, Matthew D.

“…Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. …”
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Directed evolution of phage lysins by Cheng, Xin Yi

“…However, Gram-negative lysins are inhibited under physiologically relevant conditions such as in the presence of human serum/NaCl. Directed evolution may be employed to improve the activity and overcome the limitations of lysins. …”
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Directed Evolution Methods for Enzyme Engineering by Saurabh Rajendra Nirantar

Subjects: “…directed evolution…”
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Directed evolution of split APEX peroxidase by Han, Yisu, Ph. D. Massachusetts Institute of Technology

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Continuous Directed Evolution in Mammalian Cells by Hendel, Samuel Joseph

“…Directed evolution is a powerful methodology for the creation of new biomolecules with user-desired functions. …”
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Directed Evolution of Glycan-Binding Proteins by Ward, Elizabeth M.

“…This thesis details the generation of novel GBPs from a small, hyperthermostable DNA binding protein by directed evolution. A yeast surface display method for evolution of GBPs was developed and used to generate GBPs for the recognition of mammalian glycans sialic acid and the cancer-associated disaccharide Thomsen-Friedenreich (TF) antigen. …”
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TRIZ directed evolution for automobile fuel by Hassan, Mohd Roshdi, Al-Kodami, Yaser Yahya

“…By using the tools of Directed Evolution (DE) and utilizing Level of Innovation Domains, battery development is going to be deeply illustrated. …”
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CADEE: Computer-Aided Directed Evolution of Enzymes by Beat Anton Amrein, Fabian Steffen-Munsberg, Ireneusz Szeler, Miha Purg, Yashraj Kulkarni, Shina Caroline Lynn Kamerlin

Subjects: “…computational directed evolution…”
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An Adaptable Platform for Directed Evolution in Human Cells by Berman, Chet Michael., Papa, Louis John, Hendel, Samuel J., Moore, Christopher Lawrence, Suen, Patreece H., Weickhardt, Alexander F., Doan, Ngoc Duc, Kumar, Caiden M., Butty, Vincent L. G., Shoulders, Matthew D.

“…The dynamic range for directed evolution can be enhanced to several orders of magnitude via application of a small-molecule adenoviral protease inhibitor to modulate selection pressure during directed evolution experiments. …”
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New strategies for in vivo continuous directed evolution by Papa, Louis John, III.

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Optimizing directed evolution of S. pyogenes Cas9 by Lee, Shi Mun

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Evolutionary methods in biotechnology : clever tricks for directed evolution / by Brakmann, Susanne, Schwienhorst, Andreas

Eukaryotic-driven directed evolution of Cas9 nucleases by Giulia Vittoria Ruta, Matteo Ciciani, Eyemen Kheir, Michele Domenico Gentile, Simone Amistadi, Antonio Casini, Anna Cereseto

“…EPICA is validated with CjCas9, generating an enhanced variant, UltraCjCas9, following directed evolution rounds. UltraCjCas9 is up to 12-fold more active in mammalian endogenous genomic loci, while preserving high genome-wide specificity. …”
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Models for the directed evolution of bacterial allelopathy: bacteriophage lysins by James J. Bull, Cameron Crandall, Anna Rodriguez, Stephen M. Krone

Subjects: “…Directed evolution…”
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Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer by Ron Amon, Ronit Rosenfeld, Shahar Perlmutter, Oliver C. Grant, Sharon Yehuda, Aliza Borenstein-Katz, Ron Alcalay, Tal Marshanski, Hai Yu, Ron Diskin, Robert J. Woods, Xi Chen, Vered Padler-Karavani

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Improving kinetic or thermodynamic stability of an azoreductase by directed evolution. by Vânia Brissos, Nádia Gonçalves, Eduardo P Melo, Lígia O Martins

“…Therefore its improvement is better addressed through directed evolution than by rational design approaches. In this study, five rounds of mutagenesis/recombination followed by high-throughput screening (≈10,000 clones) yielded the hit 1B6 showing a 300-fold higher half life at 50°C than that exhibited by the homodimeric wild type PpAzoR azoreductase from Pseudomonas putida MET94. …”
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Modeling the Directed Evolution of Broad Host Range Phages by James J. Bull, Holly A. Wichman, Stephen M. Krone

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Design and directed evolution of noncanonical β-stereoselective metalloglycosidases by Woo Jae Jeong, Woon Ju Song

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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors by Haroon Butt, Ayman Eid, Afaque A. Momin, Jeremie Bazin, Martin Crespi, Stefan T. Arold, Magdy M. Mahfouz

Subjects: “…Directed evolution…”
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