Integrated analysis of HSP20 genes in the developing flesh of peach: identification, expression profiling, and subcellular localization by Chunhua Zhang, Yanping Zhang, Ziwen Su, Zhijun Shen, Hongfeng Song, Zhixiang Cai, Jianlan Xu, Lei Guo, Yuanyuan Zhang, Shaolei Guo, Meng Sun, Shenge Li, Mingliang Yu

“…Additionally, to our knowledge, Prupe.3G034800.1 is the first HSP20 found in plants that has the dual targets of both the endoplasmic reticulum and nucleus. Conclusions This study provides a comprehensive understanding of PpHSP20s, lays a foundation for future analyses of the unknown function of PpHSP20 family genes in red-fleshed peach fruit and advances our understanding of plant HSP20 genes.…”
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Regulation of the apoptosis/autophagy switch by propionic acid in ventromedial hypothalamus of rats with type 2 diabetes mellitus by Larysa Natrus, Yuliia Osadchuk, Olha Lisakovska, Toralf Roch, Nina Babel, Yuliia Klys, Dmytro Labudzynskyi, Yuri Chaikovsky

“…Results: T2DM-induced apoptosis and mitoptosis, enlarged endoplasmic reticulum (ER) tubules/cisterns were observed in VMH, and accompanied by an imbalance of pro- and anti-apoptotic factors: elevation of pro-apoptotic markers Bax and caspase-3, decrease in autophagy protein LC3 and anti-apoptotic Bcl-xl. …”
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miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress by Chikako Hayashi, Takao Fukuda, Kentaro Kawakami, Masaaki Toyoda, Yuki Nakao, Yukari Watanabe, Takanori Shinjo, Tomomi Sano, Misaki Iwashita, Karen Yotsumoto, Miyu Shida, Takaharu Taketomi, Terukazu Sanui, Takeshi Uchiumi, Takashi Kanematsu, Fusanori Nishimura

“…Here, we found that the activating transcription factor (ATF)-6β, a novel miR-1260b-targeting gene, is critical for the regulation of osteoclastogenesis under endoplasmic reticulum (ER) stress. An experimental periodontal mouse model demonstrated that induction of ER stress was accompanied by enhanced ATF6β expression, and local administration of miR-1260b and ATF6β siRNA using polyethylenimine nanoparticles (PEI-NPs) significantly suppressed the periodontal bone resorption. …”
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Molecular Mechanisms and Cellular Contribution from Lung Fibrosis to Lung Cancer Development by Anna Valeria Samarelli, Valentina Masciale, Beatrice Aramini, Georgina Pamela Coló, Roberto Tonelli, Alessandro Marchioni, Giulia Bruzzi, Filippo Gozzi, Dario Andrisani, Ivana Castaniere, Linda Manicardi, Antonio Moretti, Luca Tabbì, Giorgia Guaitoli, Stefania Cerri, Massimo Dominici, Enrico Clini

“…To this purpose, IPF and LC share several cellular and molecular processes driving the progression of both pathologies such as fibroblast transition proliferation and activation, endoplasmic reticulum stress, oxidative stress, and many genetic and epigenetic markers that predispose IPF patients to LC development. …”
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The Inhibitory Role of Rab11b in Osteoclastogenesis through Triggering Lysosome-Induced Degradation of c-Fms and RANK Surface Receptors by Manh Tien Tran, Yuka Okusha, Yunxia Feng, Masatoshi Morimatsu, Penggong Wei, Chiharu Sogawa, Takanori Eguchi, Tomoko Kadowaki, Eiko Sakai, Hirohiko Okamura, Keiji Naruse, Takayuki Tsukuba, Kuniaki Okamoto

“…Rab11b was localized in early and late endosomes, Golgi complex, and endoplasmic reticulum; moreover, its overexpression enlarged early and late endosomes. …”
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Phenotypes controlled by the Brucella abortus two component system BvrR/BvrS are differentially impacted by BvrR phosphorylation by Pamela Altamirano-Silva, Jazmín Meza-Torres, Ana Mariel Zúñiga-Pereira, Sigrid Zamora-Jaen, Natalia Pietrosemoli, Gabriela Cantos, Johann Peltier, Johann Peltier, Javier Pizarro-Cerdá, Edgardo Moreno, Carlos Chacón-Díaz, Caterina Guzmán-Verri, Esteban Chaves-Olarte

“…Brucella abortus is a zoonotic pathogen whose virulence depends on its ability to survive intracellularly at the endoplasmic reticulum derived compartment. The two-component system BvrR/BvrS (BvrRS) is essential for intracellular survival due to the transcriptional control of the type IV secretion system VirB and its transcriptional regulator VjbR. …”
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Teleost fish IgM+ plasma-like cells possess IgM-secreting, phagocytic, and antigen-presenting capacities by Liting Wu, Liting Wu, Yanjian Yang, Along Gao, Jun Li, Jun Li, Jianmin Ye, Jianmin Ye

“…We analyzed the morphological and ultrastructural features of sorted IgM+ cells and found that L-gate IgM+ cells have a high nucleus–cytoplasm ratio and lymphocyte-like morphology, whereas G-M gate IgM+ cells have a small nucleus, more abundant endoplasmic reticulum, and a larger number of mitochondria, and have a plasma cell-like or macrophage-like morphology. …”
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Urinary proteome analysis of acute kidney injury in post-cardiac surgery patients using enrichment materials with high-resolution mass spectrometry by Yunpeng Bai, Yunpeng Bai, Ying Li, Zhizhong Tang, Linhui Hu, Xinyi Jiang, Xinyi Jiang, Jingchun Chen, Jingchun Chen, Sumei Huang, Sumei Huang, Sumei Huang, Kunyong Wu, Kunyong Wu, Wang Xu, Wang Xu, Wang Xu, Chunbo Chen, Chunbo Chen, Chunbo Chen, Chunbo Chen

“…According to KEGG enrichment analysis, the signal pathway of protein processing in the endoplasmic reticulum was enriched as the most up-regulated DEPs, and cell adhesion molecules were enriched as the most down-regulated DEPs. …”
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Alterations in skeletal muscle abundance of protein turnover, stress, and antioxidant proteins during the periparturient period in dairy cows fed ethyl-cellulose rumen-protected me... by Lam Phuoc Thanh, Nithat Wichasit, Yu Li, Fernanda Batistel, Wandee Tartrakoon, Claudia Parys, Jessie Guyader, Juan J. Loor

“…Compared with d 1 after calving and regardless of diet, the abundance of proteins associated with endoplasmic reticulum stress (XBP1 spliced), cell growth and survival (phosphorylated MAPK3), inflammation (transcription factor p65), antioxidant responses (KEAP1), and circadian regulation (CLOCK, PER2) of oxidative metabolism was upregulated at d 21 relative to parturition. …”
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The Activation of Reticulophagy by ER Stress through the ATF4-MAP1LC3A-CCPG1 Pathway in Ovarian Granulosa Cells Is Linked to Apoptosis and Necroptosis by Huiduo Li, Yanan Jing, Xiaoya Qu, Jinyi Yang, Pengge Pan, Xinrui Liu, Hui Gao, Xiuying Pei, Cheng Zhang, Yanzhou Yang

“…Female infertility is caused by premature ovarian failure (POF), which is triggered by the endoplasmic reticulum (ER) stress-mediated apoptosis of granulosa cells. …”
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The ER Stress Inducer <span style="font-variant: small-caps">l</span>-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca²⁺-Dependent... by Gemma Roest, Evelien Hesemans, Kirsten Welkenhuyzen, Tomas Luyten, Nikolai Engedal, Geert Bultynck, Jan B. Parys

“…Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) to reduce protein load and restore homeostasis, including via induction of autophagy. …”
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<i>Moringa oleifera</i> Improves MAFLD by Inducing Epigenetic Modifications by C. Alejandra Monraz-Méndez, Rebeca Escutia-Gutiérrez, Jonathan Samael Rodriguez-Sanabria, Marina Galicia-Moreno, Hugo Christian Monroy-Ramírez, Laura Sánchez-Orozco, Jesus García-Bañuelos, Ricardo De la Rosa-Bibiano, Arturo Santos, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez

“…Analysis of hepatic transcriptome showed down expression of mRNAs implicated in DNA response to damage, endoplasmic reticulum stress, lipid biosynthesis and insulin resistance. …”
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Enhancement of Tumor Cell Immunogenicity and Antitumor Properties Derived from Platinum-Conjugated Iron Nanoparticles by Ángela-Patricia Hernández, Laura Iglesias-Anciones, José Javier Vaquero-González, Rafael Piñol, Julio J. Criado, Emilio Rodriguez, Pablo Juanes-Velasco, Marina L. García-Vaquero, Carlota Arias-Hidalgo, Alberto Orfao, Ángel Millán, Manuel Fuentes

“…Within these strategies, first, nanotechnology has become an essential tool in oncotherapy, improving biopharmaceutical properties and providing new immunomodulatory profiles to conventional drugs mediated by activation of endoplasmic reticulum (ER) stress. Secondly, functional proteomics techniques based on microarrays have proven to be a successful method for high throughput screening of proteins and profiling of biomolecule mechanisms of action. …”
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Gut microbe-derived metabolite trimethylamine N-oxide activates PERK to drive fibrogenic mesenchymal differentiation by Seok-Jo Kim, Swarna Bale, Priyanka Verma, Qianqian Wan, Feiyang Ma, Johann E. Gudjonsson, Stanley L. Hazen, Paul W. Harms, Pei-Suen Tsou, Dinesh Khanna, Lam C. Tsoi, Nilaksh Gupta, Karen J. Ho, John Varga

“…Using cell culture systems and patient biopsies, we now show that TMAO reprograms skin fibroblasts, vascular endothelial cells, and adipocytic progenitor cells into myofibroblasts via the putative TMAO receptor protein R-like endoplasmic reticulum kinase (PERK). Remarkably, FMO3 was detected in skin fibroblasts and its expression stimulated by TGF-β1. …”
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The <i>Anemonia sulcata</i> Toxin BDS-I Protects Astrocytes Exposed to Aβ_1–42 Oligomers by Restoring [Ca²⁺]_i Transients and ER Ca²⁺ Sign... by Ilaria Piccialli, Valentina Tedeschi, Francesca Boscia, Roselia Ciccone, Antonella Casamassa, Valeria de Rosa, Paolo Grieco, Agnese Secondo, Anna Pannaccione

“…We showed that: (1) primary cortical astrocytes expressing K_V3.4 channels displayed [Ca²⁺]_i transients depending on the occurrence of membrane potential spikes, (2) BDS-I restored, in a dose-dependent way, [Ca²⁺]_i transients in astrocytes exposed to Aβ_1–42 oligomers (5 µM/48 h) by inhibiting hyperfunctional K_V3.4 channels, (3) BDS-I counteracted Ca²⁺ overload into the endoplasmic reticulum (ER) induced by Aβ_1–42 oligomers, (4) BDS-I prevented the expression of the ER stress markers including active caspase 12 and GRP78/BiP in astrocytes treated with Aβ_1–42 oligomers, and (5) BDS-I prevented Aβ_1–42-induced reactive oxygen species (ROS) production and cell suffering measured as mitochondrial activity and lactate dehydrogenase (LDH) release. …”
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Slowly reducible genetically encoded green fluorescent indicator for in vivo and ex vivo visualization of hydrogen peroxide by Piatkevich, Kiryl

“…We also demonstrated and characterized the NeonOxIrr response to H2O2 when the sensor was targeted to the matrix and intermembrane space of the mitochondria, nucleus, cell membranes, peroxisomes, Golgi complex, and endoplasmic reticulum of HEK293T cells. NeonOxIrr could reveal endogenous reactive oxygen species (ROS) production in HeLa cells induced with staurosporine but not with thapsigargin or epidermal growth factor. …”
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Art of tumor immunity: extricating hypoxia-driven immunosuppression in the solid tumor microenvironment by Tay, Apple Hui Min

“…Inhibiting hypoxia-driven immune checkpoints, endoplasmic reticulum oxidoreductase 1-alpha (ERO1a) and indoleamine 2,3-dioxygenase 1 (IDO1), modulated the PDAC secretome to upregulate antigen presentation pathways, enhance infiltration and differentiation of dendritic cells (DCs). …”
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In vitro transgenic models to elucidate the molecular mechanisms of TDP-43 pathology in amyotrophic lateral sclerosis by Mutihac, R

“…Cells expressing the mislocalized TDP M337V mutant spontaneously developed cytoplasmic punctae, while for TDP-A382T punctae were only revealed after endoplasmic reticulum (ER) stress induced by the calcium-modifying drug thapsigargin (TG). …”

Cellular Apoptosis of 4T1 Breast Cancer Cells Induced By V4-UPM Newcastle Disease Virus by Mahadi, Mahani

“…The distinct ultrastructural changes prominently seen in 4T1 cells treated with V4-UPM NDV were the apoptotic characteristics, such as, cell shrinkage and resulting spaces in between cells, membrane blebbing, shrunken nucleus and also the presence of numerous numbers of mitochondria and endoplasmic reticulum (ER). From these findings, it was confirmed that the mode of cell death induced by V4-UPM NDV, to eliminate the cancer cells is by apoptosis. …”
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Studies on neuronal proteins by Chubb, I, Chubb, I. W.

“…Secondly, there were occasions when the acetylcholinesterase-rich endoplasmic reticulum appeared to be in the process of fusing with either the axonal or chromaffin cell's plasma membrane. …”