<i>SELL</i> and <i>GUCY1A1</i> Gene Polymorphisms in Patients with Unstable Angina by Damian Malinowski, Magda Zawadzka, Krzysztof Safranow, Marek Droździk, Andrzej Pawlik

“…L-selectin is an adhesion molecule encoded by the human <i>SELL</i> gene, playing an important role in leukocyte adhesion to the endothelium and the development of inflammation. Guanylate cyclase 1 soluble subunit alpha 1 (<i>GUCY1A1</i>) is a gene that affects vasoreactivity and platelet function, thereby influencing thrombotic processes and the risk of developing thrombotic lesions in the coronary vessels. …”
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Comparison of gene expression between human and mouse iPSC-derived cardiomyocytes for stem cell therapies of cardiovascular defects via bioinformatic analysis by Ryan Bellman, Jishizhan Chen, Lidan Chen, Nikolitsa Nomikou, Janice Tsui, George Hamilton, Wenhui Song

“…Conclusion The study provides new insights into the key genes related to the iPSCs, including genes related to angiogenesis, calcification, and striated muscle, endothelium, and bone formation. Moreover, the clear differences between both mouse and human-derived iPSCs have been identified, which could be used as new evidence and guidance for developing novel targeted therapy strategies to improve the therapeutic effects of iPSC treatment in cardiovascular defects.…”
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Rnd3 suppresses endothelial cell pyroptosis in atherosclerosis through regulation of ubiquitination of TRAF6 by Yan Zhang, Zhengru Zhu, Yang Cao, Zhenyu Xiong, Yu Duan, Jie Lin, Xuebin Zhang, Mengyuan Jiang, Yue Liu, Wanrong Man, Tengfei Jia, Jiaxu Feng, Yanyan Chen, Congye Li, Baolin Guo, Dongdong Sun

“…Methods ApoeKO mice were utilized as a model for atherosclerosis. Endothelium‐specific transgenic mice were employed to disrupt the expression level of Rnd3 in vivo. …”
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The possibility of early detection of atherosclerotic vascular lesions in rheumatic diseases by A. N. Bazhenov, A. L. Maslyanskiy, E. P. Kolesova, I. N. Penin, M. D. Cheshuina, A. V. Kozlenok, O. P. Rotar

“…Dysfunction of the endothelium have registered a value of RHI < 1.64. Echocardiography and ultrasonography of carotid arteries with the assessment of the thickness of the thickness intima-media (TIM) conducted by Vivid 7 high resolution sensor 7 MHz. …”
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Factors promoting development of renal tubulointerstitial lesions in patients with diabetes mellitus by Minara Shamkhalovna Shamkhalova, Kseniya Olegovna Kurumova, Inna Igorevna Klefortova, Ivan Ivanovich Sitkin, Alexander Viktorovich Il'in, Margarita Ivanovna Arbuzova, Nikolay Petrovich Goncharov, Gul'nara Viktorovna Katsiya, Andrey Alexeevich Aleksandrov, Svetlana Semenovna Kukharenko, Marina Vladimirovna Shestakova, Ivan Ivanovich Dedov

“…Activation of profibrogeniccytokines in DM patients with CKD was closely associated with endothelial dysfunction manifest as enhanced production of blood adhesive angiogenic, thrombogenic factors (FW, PAI, VICAM, sICAM, VEGF), and endothelium-affecting factors (ADMA, homocysteine). …”
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Effect of mechanical stretching and substrate stiffness on the morphology, cytoskeleton and nuclear shape of corneal endothelial cells by Ruotian Du, Dongyan Li, Yan Huang, Hui Xiao, Jindong Xue, Jing Ji, Yun Feng, Yubo Fan

“…The expressions of the corneal endothelium-related markers ZO-1 and Na+/K+-ATPase were also evaluated by confocal immunofluorescence microscopy in the stiffness group. …”
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NTPDase1/CD39 Ectonucleotidase Is Necessary for Normal Arterial Diameter Adaptation to Flow by Julie Favre, Charlotte Roy, Anne-Laure Guihot, Annick Drouin, Manon Laprise, Marc-Antoine Gillis, Simon C. Robson, Eric Thorin, Jean Sévigny, Daniel Henrion, Gilles Kauffenstein

“…Our results highlight a key regulatory role for purinergic signaling and CD39 in endothelium-dependent short- and long-term arterial diameter adaptation to increased flow.…”
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Pericytes modulate endothelial inflammatory response during bacterial infection by Thaynara P. Carvalho, Frank A. O. Toledo, Diego F. A. Bautista, Monique F. Silva, Jefferson B. S. Oliveira, Pâmela A. Lima, Fabíola B. Costa, Noelly Q. Ribeiro, Jee-Yon Lee, Alexander Birbrair, Tatiane A. Paixão, Reneé M. Tsolis, Renato L. Santos

“…The absence of pericytes or blocking interaction between pericytes and endothelium through connexin 43 results in stronger inflammation, which shifts our understanding of pericyte biology, from a structural cell to a player in controlling inflammation.…”
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KMUP-1 Ameliorates Ischemia-Induced Cardiomyocyte Apoptosis through the NO–cGMP–MAPK Signaling Pathways by Meng-Luen Lee, Erna Sulistyowati, Jong-Hau Hsu, Bo-Yau Huang, Zen-Kong Dai, Bin-Nan Wu, Yu-Ying Chao, Jwu-Lai Yeh

“…The level of intracellular calcium was detected using-Fura2-acetoxymethyl (Fura2-AM) staining, and mitochondrial calcium with Rhod 2-acetoxymethyl (Rhod 2-AM) staining under fluorescence microscopic observation. The expression of endothelium NO synthase (eNOS), inducible NO synthase (iNOS), soluble guanylate cyclase &#945;1 (sGC&#945;1), PKG, Bcl-2/Bax ratio, ERK1/2, p38, and JNK proteins were measured by Western blotting assay. …”
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Downregulation of thrombomodulin-thrombin-activated protein C pathway as a mechanism for SARS-CoV-2 induced endotheliopathy and microvascular thrombosis by S. Agarwal, C.T. Cohen, M. Zobeck, P.M. Jacobi, S.E. Sartain

“…Thrombomodulin (TM) is an endothelial glycoprotein that plays a dual role in maintaining healthy endothelium-as a natural anticoagulant by binding thrombin to activate protein C (APC) and a negative regulator of the alternate complement pathway (AP). …”
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Changes in the levels of free sialic acid during ex vivo lung perfusion do not correlate with pulmonary function. Experimental model by Claudia Hernández-Jiménez, Javier Martínez-Cortés, J. Raúl Olmos-Zuñiga, Rogelio Jasso-Victoria, María Teresa López-Pérez, Néstor Emmanuel Díaz-Martínez, Marcelino Alonso-Gómez, Axel Edmundo Guzmán-Cedillo, Matilde Baltazares-Lipp, Miguel Gaxiola-Gaxiola, Adriana Méndez-Bernal, Adrián Polo-Jeréz, Juan Carlos Vázquez-Minero, Oscar Hernández-Pérez, Christopher O. Fernández-Solís

“…Despite its important contribution to lung reconditioning, this technique has the disadvantage of incurring high costs and can induce pulmonary endothelial injury through perfusion and ventilation. The pulmonary endothelium is made up of endothelial glycocalyx (EG), a coating of proteoglycans (PG) on the luminal surface. …”
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CD103 Expression on Regulatory and Follicular T Cells in Lymph Nodes, Bronchoalveolar Lavage Fluid and Peripheral Blood of Sarcoidosis Patients by Miriana d’Alessandro, Sara Gangi, Dalila Cavallaro, Laura Bergantini, Fabrizio Mezzasalma, Stefano Cattelan, Stefano Baglioni, Marta Abbritti, Paolo Cameli, Elena Bargagli

“…(1) Background: Sarcoidosis is a chronic multisystem disorder of unknown aetiology, driven by a T-cell mechanism allowing T-cell attachment and transmigration through the endothelium, and endorsed by the expression of an integrin alpha-E beta-7 (CD103). …”
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Haemodynamic characterisation of different endotypes in coronary artery vasospasm in reaction to acetylcholine by Rutger G.T. Feenstra, Coen K.M. Boerhout, Caitlin E.M. Vink, Janneke Woudstra, Marianne E. Wittekoek, Guus A. de Waard, Yolande Appelman, Etto C. Eringa, Koen M.J. Marques, Robbert J. de Winter, Tim P. van de Hoef, Marcel A.M. Beijk, Jan J. Piek

“…Conclusion: In reaction to low-dose acetylcholine the negative and equivocal endotype has haemodynamic changes that suggest intact endothelium. In reaction to high-dose acetylcholine the epicardial vasospasm, microvascular vasospasm and equivocal endotype have hemodynamic changes that suggest VSMC-hyperreactivity. …”
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The Nucleolar Protein Nucleophosmin Is Physiologically Secreted by Endothelial Cells in Response to Stress Exerting Proangiogenic Activity Both In Vitro and In Vivo by Anna Di Carlo, Sara Beji, Silvia Palmerio, Mario Picozza, Marco D’Agostino, Vincenzo Petrozza, Roberta Melchionna, Antonia Germani, Alessandra Magenta, Elena De Falco, Daniele Avitabile

“…NPM can be actively secreted by inflammatory cells, however its biology on endothelium remains unexplored. In this study, we show for the first time that NPM is secreted by human vein endothelial cells (HUVEC) in the early response to serum deprivation and that NPM acts as a pro-inflammatory and angiogenic molecule both in vitro and in vivo. …”
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Tetrodotoxin Decreases the Contractility of Mesenteric Arteries, Revealing the Contribution of Voltage-Gated Na⁺ Channels in Vascular Tone Regulation by Joohee Park, Coralyne Proux, William Ehanno, Léa Réthoré, Emilie Vessières, Jennifer Bourreau, Julie Favre, Gilles Kauffenstein, César Mattei, Hélène Tricoire-Leignel, Daniel Henrion, Claire Legendre, Christian Legros

“…Our data showed that these channels are expressed in both endothelium and media of aorta and MA, in which <i>scn2a</i> and <i>scn1b</i> were the most abundant transcripts, suggesting that murine vascular Na_V channels consist of Na_V1.2 channel subtype with Na_Vβ1 auxiliary subunit. …”
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Role of NADPH Oxidase 4 in Corneal Endothelial Cells Is Mediated by Endoplasmic Reticulum Stress and Autophagy by Dae Joong Ma, Jin Sun Hwang, Kyung Bo Noh, Sun-Hee Oh, Kyoung Wook Kim, Young Joo Shin

“…In an animal study, siRNA for NOX4 (siNOX4) or plasmid for NOX4 (pNOX4) was introduced into the corneal endothelium of rats by electroporation, using a square-wave electroporator (ECM830, Havard apparatus) to decrease or increase the expression of NOX4, respectively, and the rat corneas were cryoinjured through contact with a metal rod of 3 mm diameter frozen in liquid nitrogen for 10 min. …”
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Choroidal circulation disturbance is an initial factor in outer retinal degeneration in rats under simulated weightlessness by Yuxue Mu, Yuxue Mu, Dongyu Wei, Lilingxuan Yao, Xinyue Xu, Shaoheng Li, Ruidan Cao, Ruidan Cao, Tao Chen, Tao Chen, Zuoming Zhang

“…However, most of the mitochondria within the vascular endothelium underwent mild swelling, and by the fifth week, the choroidal vessels had various degrees of erythrocyte aggregation, mitochondrial swelling, and apoptosis. …”
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Age Impairs Soluble Guanylyl Cyclase Function in Mouse Mesenteric Arteries by Cheng Zhong, Minze Xu, Sengül Boral, Holger Summer, Falk-Bach Lichtenberger, Cem Erdoğan, Maik Gollasch, Stefan Golz, Pontus B. Persson, Johanna Schleifenbaum, Andreas Patzak, Pratik H. Khedkar

“…Pretreatment with L-NAME, which inhibits nitrix oxide synthases (NOS), decreased ACh-mediated vasorelaxation, whereby differences in vasorelaxation between groups disappeared. Endothelium-independent vasorelaxation by the NO donor sodium nitroprusside (SNP) was similar in both groups; however, SNP bolus application (10⁻⁶ mol L⁻¹) as well as soluble guanylyl cyclase (sGC) activation by runcaciguat (10⁻⁶ mol L⁻¹) caused faster responses in juvenile vessels. …”
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Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway by Abulaiti Abulizi, Jimilihan Simayi, Maimaitiming Nuermaimaiti, Mengyuan Han, Sendaer Hailati, Ziruo Talihati, Nulibiya Maihemuti, Muhadaisi Nuer, Nawaz Khan, Kayisaier Abudurousuli, Dilihuma Dilimulati, Nuerbiye Nueraihemaiti, Nicholas Moore, Wenting Zhou, Ainiwaer Wumaier

“…Conclusively, COM extract exerts hypolipidemic, anti-oxidative, anti-inflammatory, anti-thrombotic and vascular endothelium protective effects on atherosclerosis rat model, which may be related to the inhibition of EGFR/PI3K/AKT/GSK-3β signaling pathway.…”
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Antifibrotic and Regenerative Effects of Treamid in Pulmonary Fibrosis by Evgenii Skurikhin, Vladimir Nebolsin, Darius Widera, Natalia Ermakova, Olga Pershina, Angelina Pakhomova, Vyacheslav Krupin, Edgar Pan, Mariia Zhukova, Fedor Novikov, Lubov Sandrikina, Sergey Morozov, Aslan Kubatiev, Alexander Dygai

“…Based on these findings, we suggest that Treamid may represent a promising compound for the development of new antifibrotic agents, which are capable of stimulating regeneration of lung endothelium in IPF patients.…”
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