Anemoside B4 Inhibits Vascular Smooth Muscle Cell Proliferation, Migration, and Neointimal Hyperplasia by Dan Shan, Dan Shan, Dan Shan, Ping Qu, Chao Zhong, Chao Zhong, Luling He, Qingshan Zhang, Guoyue Zhong, Wenhui Hu, Yulin Feng, Shilin Yang, Xiao-feng Yang, Jun Yu

“…Herein, we demonstrate that B4 administration had a potent therapeutic effect in reducing neointima formation in a preclinical mouse femoral artery endothelium denudation model. Bromodeoxyuridine incorporation study showed that B4 attenuated neointimal VSMC proliferation in vivo. …”
Get full text

Priming of Cardiopulmonary Bypass with Human Albumin Decreases Endothelial Dysfunction after Pulmonary Ischemia–Reperfusion in an Animal Model by Jean Selim, Mouad Hamzaoui, Antoine Ghemired, Zoubir Djerada, Laurence Chevalier, Nicolas Piton, Emmanuel Besnier, Thomas Clavier, Anaïs Dumesnil, Sylvanie Renet, Paul Mulder, Fabien Doguet, Fabienne Tamion, Benoît Veber, Jérémy Bellien, Vincent Richard, Jean-Marc Baste

“…We employed a CPB model with three different primings, permitting femoral–femoral assistance with left pulmonary hilum ischemia for IR. Pulmonary endothelium-dependent relaxation to acetylcholine was significantly decreased in the IR-CPB-GF group (11.9 ± 6.2%) compared to the IR-CPB-HA group (52.8 ± 5.2%, <i>p</i> < 0.0001), the IR-CPB-HSL group (57.7 ± 6.3%, <i>p</i> < 0.0001) and the sham group (80.8 ± 6.5%, <i>p</i> < 0.0001). …”
Get full text

Comprehensive treatment of the macular edema in patients with retinal vein occlusion and hemostasis system disturbances by T.V. Shelkovnikova, Kh.P. Takhchidi, G.V. Vavin, N.Yu. Barkova, N.Yu. Shishlyannikova

“…LA significantly reduces the thromboresistance of the endothelium of the retinal microvasculature and enhances hypercoagulation, breaks the natural regulation of the hemostasis system and aggravates retinal ischemia.…”
Get full text

Inflammation-induced TRPV4 channels exacerbate blood–brain barrier dysfunction in multiple sclerosis by Cathrin E. Hansen, Alwin Kamermans, Kevin Mol, Kristina Berve, Carla Rodriguez-Mogeda, Wing Ka Fung, Bert van het Hof, Ruud D. Fontijn, Susanne M. A. van der Pol, Laura Michalick, Wolfgang M. Kuebler, Boyd Kenkhuis, Willeke van Roon-Mom, Wolfgang Liedtke, Britta Engelhardt, Gijs Kooij, Maarten E. Witte, Helga E. de Vries

“…Moreover, TRPV4 activity mediated T cell extravasation across the brain endothelium. Conclusion Collectively, our findings suggest a novel role for endothelial TRPV4 in MS, in which enhanced expression contributes to MS pathogenesis by driving BBB dysfunction and immune cell migration.…”
Get full text

Contact System Activation in Plasma from Dengue Patients Might Harness Endothelial Virus Replication through the Signaling of Bradykinin Receptors by Sharton V. A. Coelho, Naiara M. Rust, Lucas Vellasco, Michelle P. Papa, Aline S. G. Pereira, Matheus Ferreira da Silva Palazzo, Maria Aparecida Juliano, Simone M. Costa, Ada M. B. Alves, Marli T. Cordeiro, Ernesto T. A. Marques, Júlio Scharfstein, Luciana B. de Arruda

“…In summary, our study suggests that contact/KKS activation followed by BK-induced enhancement of DENV replication in the endothelium may underlie microvascular pathology in dengue.…”
Get full text

Blood coagulation and aortic wall integrity in rats with obesity-induced insulin resistance by O. S. Dziuba, V. O. Chernyshenko, Ie. A. Hudz, L. O. Kasatkina, T. M. Chernyshenko, P. P. Klymenko, H. V. Kosiakova, T. M. Platonova, N. M. Hula, E. V. Lugovskoy

“…Histological analysis identified the disruption of aorta endothelium and tendency for the thickening of the aorta wall in the group with obesity-induced IR compared to the group of control rats. …”
Get full text

Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca2+ signaling and... by Barbara Lisec, Tim Bozic, Iva Santek, Bostjan Markelc, Milka Vrecl, Robert Frangez, Maja Cemazar

“…Abstract Background Disruption of Ca2+ homeostasis after calcium electroporation (CaEP) in tumors has been shown to elicit an enhanced antitumor effect with varying impacts on healthy tissue, such as endothelium. Therefore, our study aimed to determine differences in Ca2+ kinetics and gene expression involved in the regulation of Ca2+ signaling and homeostasis, as well as effects of CaEP on cytoskeleton and adherens junctions of the established endothelial cell lines EA.hy926 and HMEC-1. …”
Get full text

Impaired Endothelial Function in Convalescent Phase of COVID-19: A 3 Month Follow Up Observational Prospective Study by Luca Santoro, Lorenzo Falsetti, Vincenzo Zaccone, Antonio Nesci, Matteo Tosato, Bianca Giupponi, Maria Cristina Savastano, Gianluca Moroncini, Antonio Gasbarrini, Francesco Landi, Angelo Santoliquido, on behalf of Gemelli against COVID-19 Post-Acute Care Study Group

“…Evidence regarding COVID-19 middle- and long-term consequences on endothelium are still lacking. Our study aimed to evaluate if COVID-19 severity could significantly affect the endothelial function after three months from the acute phase. …”
Get full text

Analysis of the Increase of Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression and the Effect of Exposure in a Hyperbaric Chamber on VCAM-1 in Human Blood Serum: A Cross-Section... by Katarzyna Van Damme-Ostapowicz, Mateusz Cybulski, Mariusz Kozakiewicz, Elżbieta Krajewska-Kułak, Piotr Siermontowski, Marek Sobolewski, Dorota Kaczerska

“…Exposure may result in activation of the endothelium.…”
Get full text

Novel fibrin-fibronectin matrix accelerates mice skin wound healing by Carlos Poblete Jara, Ou Wang, Thais Paulino do Prado, Ayman Ismail, Frank Marco Fabian, Han Li, Licio A. Velloso, Mark A. Carlson, William Burgess, Yuguo Lei, William H. Velander, Eliana P. Araújo

“…The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. …”
Get full text

Microenvironment commits breast tumor ECs to dedifferentiation by micro-RNA-200-b-3p regulation and extracellular matrix remodeling by Kinga Wilkus-Adamczyk, Kinga Wilkus-Adamczyk, Klaudia Brodaczewska, Aleksandra Majewska, Aleksandra Majewska, Claudine Kieda, Claudine Kieda

“…This study aimed to evidence the influences of the tumor microenvironment on the global gene expression pattern characteristic for ECs and the distinct responses displayed by tumor-derived ECs in comparison to the healthy endothelium during endothelial to mesenchymal transition (EndMT) and its regulation by miR-200-b-3p.Methodology: Immortalized lines of ECs from the same patient with breast cancer, healthy breast tissue (HBH.MEC), and primary tumor (HBCa.MEC) were used. …”
Get full text

Essential Oils from Mediterranean Plants Inhibit In Vitro Monocyte Adhesion to Endothelial Cells from Umbilical Cords of Females with Gestational Diabetes Mellitus by Valeria Schiavone, Tea Romasco, Nadia Di Pietrantonio, Stefania Garzoli, Carola Palmerini, Pamela Di Tomo, Caterina Pipino, Domitilla Mandatori, Rossella Fioravanti, Elena Butturini, Manuela Sabatino, Maria Pompea Antonia Baldassarre, Rino Ragno, Assunta Pandolfi, Natalia Di Pietro

“…Hence, the aim of the present study was to investigate the potential anti-inflammatory effect of anise and laurel EOs (AEO and LEO) on endothelial cells isolated from the umbilical cord vein of females with gestational diabetes mellitus (GDM-HUVEC), which is a suitable in vitro model to reproduce the pro-inflammatory phenotype of a diabetic endothelium. For this purpose, the Gas Chromatographic/Mass Spectrometric (GC-MS) chemical profiles of AEO and LEO were first analyzed. …”
Get full text

Pharmacoeconomic analysis of ranibizumab and aflibercept for treatment of diabetic macular edema by A. E. Cheberda, D. Yu. Belousov, M. M. Shishkin

“…Significant progress in treating DME has been obtained after the role of vascular endothelium growth factor (VEGF) in the disease’s pathophysiology became more understood, with several powerful clinical options emerging as result. …”
Get full text

PHARMACOTHERAPEUTIC STRATEGIES FOR ENDOTHELIAL DYSFUNCTION CORRECTION WITH USE OF STATINES IN SYNDROME OF SYSTEMIC INFLAMMATORY RESPONSE by Tatiana Denisyuk

“…Modeling of L-NAME-induced pathology and further evaluation of endothelial and endothelium-independent vascular reactions were carried through according to a standard protocol. …”
Get full text

Endothelial dysfunction due to the inhibition of the synthesis of nitric oxide: Proposal and characterization of an in vitro cellular model by Fernanda Cardoso da Silva, Fernanda Cardoso da Silva, Bruna Juber de Araújo, Bruna Juber de Araújo, Carina Santos Cordeiro, Vinícius Marques Arruda, Bruno Quintanilha Faria, Joyce Ferreira Da Costa Guerra, Thaise Gonçalves De Araújo, Cristina Ribas Fürstenau

“…The vascular endothelium plays a pivotal role in the maintenance of vascular homeostasis, mediated by vasoactive molecules produced by endothelial cells. …”
Get full text

Acute effects of exercise with blood flow restriction on endothelial function in healthy young and older populations: a systematic review by Gustavo Gonçalves Cardozo, Karynne Grutter Lopes, Daniel Alexandre Bottino, Maria das Graças Coelho de Souza, Eliete Bouskela, Paulo Farinatti, Ricardo Brandão de Oliveira

“…The studies included healthy individuals who underwent assessments of endothelial function before and after experimental protocols through endothelium-dependent flow-mediated dilatation. In total, 4890 studies were screened, and 6 studies of moderate-to-high methodological quality (Physiotherapy Evidence Database scores 6 - 10) including 82 subjects (aged 24 - 68 years) were eligible. …”
Get full text

Effects of diet on blood rheological indices, cytokine concentrations, and emergence of metabolic disorders in the persons with increased body mass index by P. S. Novikov, N. A. Cherevko, V. V. Klimov, S. E. Kondakov, M. Yu. Rozenshteyn, A. Z. Rozenshteyn, E. A. Motlokhova, D. S. Zagreshenko

“…Prevention of food dysadaptation reactions and the studies of control mechanisms of immune tolerance to food antigens is of special scientific interest, thus providing available anti-inflammatory tools for correcting increased permeability of the intestinal epithelium and vascular endothelium associated with development of MS. Nutritional dysadaptation occurs due to inappropriate diet being mediated by the geno-phenotypic characteristics of digestive enzymes and immune system which control the efficiency of food digestion.Immunological control of digestion, including dynamic maintenance of tolerance to food antigens, is carried out at two levels of immune system: innate response with functional involvement of microbiota, and adaptive response, represented by cellular and humoral mechanisms associated with molecular epitopes and critical mass of persistent food antigens which are present in immunologically competent areas of small intestine, due to changing permeability of intestinal barrier and transcytosis processes. …”
Get full text

Thapsigargin-resistant thiacalix[4]arene C-1087-sensitive component of the contractile activity in rat myometrium reflects the functioning of plasma membrane calcium pump by Olga Tsymbalyuk, Tetyana Veklich, Roman Rodik, Sergiy Karakhim, Sergiy Vyshnevskyi, Vitaly Kalchenko, Sergiy Kosterin

“…The contractile activity in the preparations of longitudinal SM of uterine horns with preserved endothelium was registered in the isometric mode. To determine the changes in [Ca2+]i level, myocytes were treated with probes Hoechst 33342 (to test the nucleus of the cell) and fluo-4 AM (to test the change in Са2+-concentration in the cell). …”
Get full text

Therapeutic efficacy of intra-arterial administration of induced pluripotent stem cells-derived neural progenitor cells in acute experimental ischemic stroke in rats by D. D. Namestnikova, I. L. Gubskiy, D. I. Salikhova, G. E. Leonov, K. K. Sukhinich, P. A. Melnikov, D. A. Vishnevskiy, E. A. Cherkashova, A. N. Gabashvili, T. B. Bukharova, V. V. Burunova, T. Kh. Fatkhudinov, V. P. Chekhonin, L. V. Gubsky, S. L. Kiselev, D. V. Goldstein, K. N. Yarygin

“…Some cells interacted with the capillary endothelium and probably penetrated through the blood-brain barrier.Conclusion. …”
Get full text

Fibroblast activation in response to TGFβ1 is modulated by co-culture with endothelial cells in a vascular organ-on-chip platform by Rebeccah J. Luu, B. Christopher Hoefler, Ashley L. Gard, Casey R. Ritenour, Miles T. Rogers, Ernest S. Kim, Jonathan R. Coppeta, Brian P. Cain, Brett C. Isenberg, Hesham Azizgolshani, Oscar R. Fajardo-Ramirez, Guillermo García-Cardeña, Matthew P. Lech, Lindsay Tomlinson, Joseph L. Charest, Corin Williams

“…A high-throughput microfluidic platform, PREDICT96, that was capable of imparting physiologically relevant fluid shear stress on the cultured endothelium was utilized.Results: We first studied the activation response of several perivascular cell types and selected a cell source, human dermal fibroblasts, that exhibited medium-level activation in response to TGFβ1. …”
Get full text