Tissue-Specific Ferritin- and GFP-Based Genetic Vectors Visualize Neurons by MRI in the Intact and Post-Ischemic Rat Brain by Marina Y. Khodanovich, Andrey E. Akulov, Tatyana V. Anan’ina, Marina S. Kudabaeva, Anna O. Pishchelko, Elena P. Krutenkova, Nikolay M. Nemirovich-Danchenko, Mikhail V. Svetlik, Yana A. Tumentceva, Chris Van den Haute, Rik Gijsbers, Veronique Daniëls, Irina Thiry, Alexandra G. Pershina, Maria M. Shadrina, Anna V. Naumova

“…Expression of the enhanced green fluorescent protein (eGFP) was used as an expression control (AAV-pDCX-eGFP). …”
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Clinical Diagnostic Performance of Droplet Digital PCR for Suspected Bloodstream Infections by Ke Lin, Yuanhan Zhao, Bin Xu, Shenglei Yu, Zhangfan Fu, Yi Zhang, Hongyu Wang, Jieyu Song, Mingxiang Fan, Yang Zhou, Jingwen Ai, Chao Qiu, Haocheng Zhang, Wenhong Zhang

“…Nucleic acid was extracted from the blood samples, and a 5-fluorescent-channel droplet digital PCR assay was performed and then fed back with the pathogen and its copies. …”
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Treatment of complex industrial wastewaters containing ammonium and phenolic compounds using granular sludge in continuous airlift reactors by Zulkifly, Jemaat

“…The achieved nitrogen loading rate (NLRv) and o-cresol loading rate (oCLRv) were ca. 1.1 g N L 1d 1 and 0.11 g o-cresol Ld', respectively. Analysis of fluorescent in-situ hybridization (FISH) indicated that Acinetobacter genus, betaproteobacterial ammoniaoxidizing bacteria (f3AOB) and Nitrobacter sp. were identified into the granules. …”
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2036 Extracellular matrix as a novel approach to glioma therapy by Mark H. Murdock, Jordan T. Chang, George S. Hussey, Nduka M. Amankulor, Johnathan A. Engh, Stephen F. Badylak

“…Darkfield time-lapse video of wells containing Nucview dye, fluorescent upon cleavage by active caspase-3, confirmed that within 12 hours most primary glioma cells underwent apoptosis while CHME5 and HFF did not. …”
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In vitro antiviral activity of Chamaecrista nictitans (Fabaceae )against herpes simplex virus: Biological characterization of mechanisms of action by Libia Herrero Uribe, Esteban Chaves Olarte, Giselle Tamayo Castillo

“…Western blot and immunofluorescence techniques were used to elucidate the antiviral mechanism of the extract by infecting Vero cells with the virus at different times and monitoring the synthesis of viral proteins.A 60 kDa protein was detected at 2 hr and 8 hr post-infection but no additional proteins were synthesized at later time intervals,and cytopathic effect was not observed after 24 hr.This result indicates that the extract acts at the intracellular level in order to inhibit late transcription.However,it does not inhibit transcription/translation of early viral proteins.These results were confirmed by immunofluorescence experiments.A strong fluorescent signal was observed in control cell monolayers at 24 hr post infection,accompanied with a clear cytopathic effect.In contrast,in the presence of acyclovir or the extract,cells showed very discrete immunofluorescence,characterized by a punctuated pattern, and no cytopathic effect was observed.Neutralization assays were performed using pre-incubation of virus with either specific herpes simplex-1 antiserum,200 mu g/ml of the extract or 20 mu g /ml of acyclovir.After 1 hr incubation,cells were infected and monitored for cytopathic effect.Only the virus treated with acyclovir showed viral activity,while no cytopathic effect was induced by samples of virus incubated with the extract.It is concluded that the extract inhibits both the attachment of the virus to the cell and the secondary transcription of the virus within the cells.Chemical characterization of the extract showed the presence of tannins.Rev.Biol.Trop.52(3):807-816. …”
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Lighting a building with a single bulb : toward a system for illumination in the 21st c.; or, A centralized illumination system for the efficient decoupling and recovery of lightin... by Levens, Kurt Antony, 1961-

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Light-matter interaction in electrically driven perovskite light emitting metasurfaces by Wang, Yutao

“…Over the past decade, light-emitting diodes (LEDs) based on III-V and organic semiconductor have transformed the landscape of displays and ambient lighting, supplanting traditional technologies such as liquid-crystal and plasma displays, incandescent bulbs, and fluorescent lights. While LEDs boast high luminance and energy efficiency, they fall short of fulfilling the needs of emerging immersive visual technologies. …”
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Pathology and pathogenesis of Brucella melitensis infection in bucks by Nasruddin, Nurrul Shaqinah

“…The cells were then harvested at 0, 30, 60 and 120 minutes post-incubation and stained with Acridine orange and Crystal violet for viewing under fluorescent microscope to determine the phagocytosis index rate and intracellular killing index. …”
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Characterization of dentatin isolated from Clausena excavate and its potential use for treatment of human breast and prostate cancers by Ishag, Ismail Adam Arbab

“…Significant increase in chromatin condensation in the cell nucleus was observed in the fluorescent analysis. The apoptosis was confirmed by reduced colony of cells in clonogenic assay and increased cellular DNA breaks on treated cells observed as ladder. …”
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Mechanism of methicillin-resistant Staphylococcus aureus biofilm degradation by bacteriophages by Dakheel, Khulood Hamid

“…This was further assayed using an in vitro static biofilm assay in microtiter-plates and in vitro visualization of the biofilm architecture in situ by labeling biofilm with two fluorescent stains, SYTO 9 and propidium iodide then performing confocal laser scanning microscopy (CLSM) analysis. …”
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Development of murine immune responses to H5 DNA vaccine with IRF3 as genetic adjuvant and HIV Tat-conjugated pamam dendrimer as the delivery system by Bahadoran, Azadeh

“…In this study the immune responses of a new recombinant DNA vaccine encoding the H5 of avian influenza virus (AIV), green fluorescent protein (GFP) and Mus musculus interferon regulatory factor 3 (IRF3) genes (pBud-H5-GFP-IRF3) delivered via a platform for DNA delivery were evaluated. …”
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Marking Effect of Coded Wire Tag on Released Juveniles of Black Rockfish (Sebastes schlegelii) by Ying LI, Yongjiang XU, Aijun CUI, Yan JIANG, Bin WANG, Xuezhou LIU, Yunchen TIAN

“…At present, otolith fluorescent labeling is the only reported marker technology that can be used for the proliferation and release of black rockfish. …”
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Combined Effects of Elevated Temperature and Polystyrene Microplastics on Hemocyte Function, Immune-Related Gene Expression, and Energy Metabolism of Crassostrea gigas by Yunchao DU, Jingying REN, Jia TENG, Jianmin ZHAO, Tianyu ZHANG, Qing WANG

“…In the current study, oysters were exposed to three levels of microplastics (no microplastics, 6 μm microplastics: SPS-MPs, and 50~60 μm microplastics: LPS-MPs) and two temperature levels (20 ℃ and 25 ℃) for 21 days, and the phagocytosis rate and reactive oxygen species (ROS) content of hemocytes, glycogen content in digestive glands, and immune-related gene expression in digestive glands and gills were examined on the 21st day. 2', 7'-Dichlorodihydrofluorescein diacetate and fluorescent microspheres were used to measure the ROS content and phagocytosis ratein hemocytes of C. gigas by flow cytometry, respectively. …”
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Lipolytic activity from bacteria prospected in polluted portuary sites by Kaori Levy Fonseca

“…Rhodamine is a dye which together with fatty acids released by the hydrolysis of triacylglycerols, forms a fluorescent complex when exposed to ultraviolet light. …”
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Role of cell-cell signaling, differentiation and fitness in multi-species biofilms by Lee, Kelvin Kai Wei

“…The three bacterial species were stably labeled with three different fluorescent proteins using a Tn7 transposon expression system. …”
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Control of size and charge selectivity in amphiphilic graft copolymer nanofiltration membranes by Lovell, Nathan Gary

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Role of cavitation, surfactants, and their synergism in transdermal sonophoresis by Polat, Bariș E

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Reaction and transport processes in OMCVD : selective and group III-nitride growth by Mihopoulos, Theodoros, 1969-

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High power gallium nitride light-emitting diodes for efficient solid state lighting and displays by Ji, Yun

“…The GaN based white LED has currently achieved reasonably high efficacy and displays a huge potential of replacing the incandescent and fluorescent lamps in the current market. However, the performance of InGaN/GaN LED light sources is still limited by some technical issues. …”
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Development of Real-Time Polymerase Chain Reaction Assays for the Detection and Differentiation of Infectious Bursal Disease Virus Subtypes by Kong, Lih Ling

“…For the Taqman based duplex real-time PCR assay development, a new set of primers FWDC and RVSC were designed from the conserved region of VP4 of both very virulent and classical strains. A dual-labeled fluorescent probe each specific for very virulent IBDV (ProVV) and vaccine IBDV (ProCL) were designed The performance of the developed Taqman assay was compared with other PCR methods namely conventional RT-PCR and previously developed SYBR Green I assay. …”
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