QPOLE: A Quick, Simple, and Cheap Alternative for POLE Sequencing in Endometrial Cancer by Multiplex Genotyping Quantitative Polymerase Chain Reaction by Anne Sophie V.M. Van den Heerik, Natalja T. Ter Haar, Lisa Vermij, Jan J. Jobsen, Mariel Brinkhuis, Suzan M. Roothaan, Alicia Leon-Castillo, Gitte Ortoft, Estrid Hogdall, Claus Hogdall, Tom Van Wezel, Ludy C.H.W. Lutgens, Marie A.D. Haverkort, Jas Khattra, Jessica N. McAlpine, Carien L. Creutzberg, Vincent T.H.B.M. Smit, C. Blake Gilks, Nanda Horeweg, Tjalling Bosse

“…Three assays, QPOLE-frequent for the most common mutations and QPOLE-rare-1 and QPOLE-rare-2 for the rare variants, were developed and optimized using DNA extracted from formalin-fixed paraffin-embedded tumor tissues. The simplicity of the design enables POLE status assessment within 4-6 hours after DNA isolation. …”
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Mechanism-based rational design of cisplatin analogues by Barnes, Katie R., 1978-

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Formulation of liposome-encapsulated piroxicam for improved therapeutic efficacies and reduced systemic toxicities by Chiong, Hoe Siong

“…The acetic acid-induced abdominal writhing test, formalin-induced paw-licking test as well as carrageenan-induced mechanical and thermal hyperalgesia test evidenced an increased and longer lasting peripherally-mediated antinociceptive activities by present liposomal piroxicam formulation. …”

Over-Expression of Biomolecules Inphosphatidylinositol-3-Kinaseiakt Signaling Pathway Inbreast Cancer by Loh, Hui Woon

“…By employing the immunohistochemical method, the expression of several key regulators or related biomolecules of the PI3WAkt signaling pathways in 43 archived formalin fixed, paraffin embedded tissues of surgically resected breast carcinoma specimens from 1999 to 2002, were studied. …”
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Elucidation of the WNT & AKT/Phosphoinositide-3-Kinase Pathways in Colorectal Carcinoma by Khor, Tin Oo

“…By employing immunohistochemical method, the expressIOn and correlation of several key regulators or related biomolecules of the Wnt and PI3K1Akt signalling pathways in 47 archival formalin fixed, paraffin embedded tissues of surgically resected colorectal cancer (CRC) specimens performed at Kuala Lumpur Hospital (KLH) between 1999 and 2000, were studied. …”
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Development of recombinant outer membrane proteins vaccine and its immunoprotective ability against Vibrio alginolyticus in hybrid grouper (Epinephelus fuscoguttatus Forsskal x Epi... by Rosli, Nehlah

“…The expressed target proteins were then detected by His-tag monoclonal antibody using SDS-PAGE and Western blotting, in which the presence of fusion proteins at 48.3 kDa and 40.3 kDa for OmpK and OmpW respectively, were confirmed. For vaccination, formalin killed whole-cell of V. alginolyticus strain VA2 was prepared at concentration of 1.59 x 107 and 8.3 x 107 CFU/ml for OmpK and OmpW respectively. …”
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Morphological development of Hilsa shad [Tenualosa ilisha (F. Hamilton, 1822)] larvae from Meghna Estuary, Bangladesh by Riar, Md. Golam Sajed

“…Collected samples were preserved in 5% formalin and transported to the laboratory. Morphological characteristics of the larvae and the mouth and gut morphology of larvae and early juvenile stages were examined using Keyence Digital Microscope (VHX-500). …”
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Scoring system for lesions induced by different strains of infectious bursal disease virus in chicken by Hussein, Elawad A., Bejo, Mohd Hair, Omar, Abdul Rahman, Liew, Pit Sze, Saeed, Mohammed Ibrahim, Arshad, Siti Suri, Awad, Elmutaz Atta, Ideris, Aini

“…In all previous experiments, tissues of bursa of Fabricius, caecal tonsil, liver, kidney, spleen, junction of proventriculus and gizzard, intestine, muscle and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin and sectioned. HS staining was applied. …”

Bladder cancer patients have increased NETosis and impaired DNaseI-mediated NET degradation that can be therapeutically restored in vitro by Raquel Herranz, Julia Oto, Marta Hueso, Emma Plana, Emma Plana, Fernando Cana, María Castaño, Lourdes Cordón, David Ramos-Soler, Santiago Bonanad, Santiago Bonanad, César D. Vera-Donoso, Manuel Martínez-Sarmiento, Pilar Medina

“…Herein, we aimed to evaluate the occurrence of increased NETosis in plasma and tumor tissue of BC patients, to ascertain whether it is mediated by a reduced DNaseI activity and degradation, and to in vitro explore novel therapeutic interventions.MethodsWe recruited 71 BC patients from whom we obtained a plasma sample before surgery and a formalin-fixed paraffin embedded tumor tissue sample, and 64 age- and sex-matched healthy controls from whom we obtained a plasma sample. …”
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Analysis of Selected Nutritional Parameters in Patients with HPV-Related and Non-HPV-Related Oropharyngeal Cancer before and after Radiotherapy Alone or Combined with Chemotherapy by Adam Brewczyński, Beata Jabłońska, Agnieszka Maria Mazurek, Jolanta Mrochem-Kwarciak, Sławomir Mrowiec, Mirosław Śnietura, Marek Kentnowski, Anna Kotylak, Zofia Kołosza, Krzysztof Składowski, Tomasz Rutkowski

“…Confirmation of HPV etiology was obtained from FFPE (formalin-fixed, paraffin-embedded) tissue material and/or extracellular circulating HPV DNA. …”
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Next-Generation Sequencing Analysis of Pancreatic Cancer Using Residual Liquid Cytology Specimens from Endoscopic Ultrasound—Guided Fine-Needle Biopsy: A Prospective Comparative St... by Hiromichi Iwaya, Akihide Tanimoto, Koshiro Toyodome, Issei Kojima, Makoto Hinokuchi, Shiroh Tanoue, Shinichi Hashimoto, Machiko Kawahira, Shiho Arima, Shuji Kanmura, Toshiaki Akahane, Michiyo Higashi, Shinsuke Suzuki, Shinichi Ueno, Takao Ohtsuka, Akio Ido

“…When stratified with a variant allele frequency (VAF) > 10% tumor burden, the NGS success rate was 76.7% (<i>n</i> = 23) in formalin-fixed paraffin-embedded (FFPE), 83.3% (<i>n</i> = 25) in LBC, and 76.7% (<i>n</i> = 23) in frozen samples. …”
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Benzophenone Derivatives with Histamine H₃ Receptor Affinity and Cholinesterase Inhibitory Potency as Multitarget-Directed Ligands for Possible Therapy of Alzheimer’s Di... by Justyna Godyń, Paula Zaręba, Dorota Stary, Maria Kaleta, Kamil J. Kuder, Gniewomir Latacz, Szczepan Mogilski, David Reiner-Link, Annika Frank, Agata Doroz-Płonka, Agnieszka Olejarz-Maciej, Sylwia Sudoł-Tałaj, Tobias Nolte, Jadwiga Handzlik, Holger Stark, Anna Więckowska, Barbara Malawska, Katarzyna Kieć-Kononowicz, Dorota Łażewska, Marek Bajda

“…Therefore, we investigated the in vivo activity of compound <b>6</b> in the Passive Avoidance Test and the Formalin Test. While compound <b>6</b> did not show a statistically significant influence on memory and learning, it showed analgesic properties in both acute (ED₅₀ = 20.9 mg/kg) and inflammatory (ED₅₀ = 17.5 mg/kg) pain.…”
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Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study by Umberto Malapelle, Paola Parente, Francesco Pepe, Caterina De Luca, Pellegrino Cerino, Claudia Covelli, Mariangela Balestrieri, Gianluca Russo, Antonio Bonfitto, Pasquale Pisapia, Fabiola Fiordelisi, Maria D’Armiento, Dario Bruzzese, Fotios Loupakis, Filippo Pietrantonio, Maria Triassi, Matteo Fassan, Giancarlo Troncone, Paolo Graziano

“…Our aim was to evaluate pre-analytical factors, such as age of formalin-fixed and paraffin-embedded (FFPE) block, neoplastic cell percentage, mucinous component, and DNA integrity, that may influence the accuracy of MSI testing and assess the concordance between three different MSI evaluation approaches. …”
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Could miRNA Signatures be Useful for Predicting Uterine Sarcoma and Carcinosarcoma Prognosis and Treatment? by Laura Gonzalez dos Anjos, Bruna Cristine de Almeida, Thais Gomes de Almeida, André Mourão Lavorato Rocha, Giovana De Nardo Maffazioli, Fernando Augusto Soares, Isabela Werneck da Cunha, Edmund Chada Baracat, Katia Candido Carvalho

“…This study aimed to assess the expression profile of 84 cancer-related miRNAs and to evaluate their correlation with clinical pathological features. Eighty-two formalin-fixed paraffin-embedded (FFPE) samples were selected. …”
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Genomically matched therapy in refractory colorectal cancer according to ESMO Scale for Clinical Actionability of Molecular Targets: experience of a comprehensive cancer centre net... by Núria Mulet Margalef, Carmen Castillo, Miguel Mosteiro, Xavier Pérez, Susana Aguilar, Fiorella Ruíz‐Pace, Marta Gil, Carmen Cuadra, José Carlos Ruffinelli, Mercedes Martínez, Ferran Losa, Gema Soler, Àlex Teulé, Roser Castany, Rosa Gallego, Andrea Ruíz, Elena Garralda, Elena Élez, Ana Vivancos, Josep Tabernero, Ramon Salazar, Rodrigo Dienstmann, Cristina Santos Vivas

“…Next‐generation sequencing (NGS) analysis from formalin‐fixed paraffin‐embedded tumour samples was performed. …”
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The Scarface Score: Deciphering Response to DNA Damage Agents in High-Grade Serous Ovarian Cancer—A GEICO Study by Antonio Fernández-Serra, Raquel López-Reig, Raúl Márquez, Alejandro Gallego, Luís Miguel de Sande, Alfonso Yubero, Cristina Pérez-Segura, Avinash Ramchandani-Vaswani, María Pilar Barretina-Ginesta, Elsa Mendizábal, Carmen Esteban, Fernando Gálvez, Ana Beatriz Sánchez-Heras, Eva María Guerra-Alía, Lydia Gaba, María Quindós, Isabel Palacio, Jesús Alarcón, Ana Oaknin, Jessica Aliaga, Marta Ramírez-Calvo, Zaida García-Casado, Ignacio Romero, José Antonio López-Guerrero

“…In this study, we have developed the Scarface score, an integrative algorithm based on genomic and transcriptomic data obtained from the NGS analysis of a prospective GEICO cohort of 190 formalin-fixed paraffin-embedded (FFPE) tumor samples from patients diagnosed with HGSOC with a median follow up of 31.03 months (5.87–159.27 months). …”
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A novel therapeutic effect of mannitol-rich extract from the brown seaweed Sargassum ilicifolium using in vitro and in vivo models by Amal D Premarathna, Rando Tuvikene, MNR Somasiri, MLWP De Silva, Ranjith Adhikari, TH Ranahewa, RRMKK Wijesundara, SK Wijesekera, IPGHU Dissanayake, Phurpa Wangchuk, Vitalijs Rjabovs, Anura P Jayasooriya, RPVJ Rajapakse

“…Wound healing properties were measured and wound tissue samples were collated, formalin-fixed, wax-embedded, stained (Hematoxylin and Eosin; Van Gieson) and examined for the healing process. …”
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The apolipoprotein B and apolipoprotein A-I Ratio serves as a strong prognostic factor for the overall survival of patients with colorectal cancer by Yangxun Pan, Yangxun Pan, Yangxun Pan, Zhiwei Ye, Zhiwei Ye, Yihong Ling, Yihong Ling, Lingheng Kong, Lingheng Kong, Chenwei Wang, Chenwei Wang, Gong Chen, Gong Chen, Desen Wan, Desen Wan, Minshan Chen, Minshan Chen, Dandan Hu, Dandan Hu

“…The present study investigated the characteristics of lipid metabolism parameters in CRC patients with or without metastases and identified the independent prognostic factors of long-term prognosis.MethodsThe clinicopathological data of 231 CRC patients along with 259 formalin-fixed paraffin-embedded samples with or without liver or lung metastasis were retrieved and stained for apolipoprotein B (apoB) via immunohistochemistry (IHC) in our center. …”
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Classical osteoblastoma, atypical osteoblastoma, and osteosarcoma: a comparative study based on clinical, histological, and biological parameters Osteoblastoma clássico, osteoblast... by Cláudia Regina Gomes Cardim Mendes de Oliveira, Berenice Bilharino Mendonça, Olavo Pires de Camargo, Emilia Modolo Pinto, Sérgio Antonio Barbosa Nascimento, Maria do Rosario D. O. Latorre, Maria Claúdia Nogueira Zerbini

“…Comparative immunohistochemical and clinical analysis was performed in 17 cases of patients with high-grade osteosarcoma. Formalin-fixed, paraffin-embedded archival tissue was immunostained for p53 and proliferation cell nuclear antigen. …”
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Association between gene expression signatures and clinical outcomes of pembrolizumab versus paclitaxel in advanced gastric cancer: exploratory analysis from the randomized, contro... by Kohei Shitara, Kei Muro, Razvan Cristescu, Michael Nebozhyn, Andrey Loboda, Hyun Cheol Chung, Wasat Mansoor, Mustafa Ozguroglu, Mario Mandala, Maria Di Bartolomeo, Christian Caglevic, Julie Kobie, Min-Hee Ryu, Z Alexander Cao, Tomasz Olesinski, Eray Goekkurt, Raymond S McDermott, Zev A Wainberg, Chie-Schin Shih

“…This prespecified exploratory analysis was conducted to evaluate associations between tumor gene expression signatures and clinical outcomes in the phase III KEYNOTE-061 trial.Methods Using RNA sequencing data obtained from formalin-fixed, paraffin-embedded baseline tumor tissue samples, we evaluated the 18-gene T-cell-inflamed gene expression profile (TcellinfGEP) and 10 non-TcellinfGEP signatures (angiogenesis, glycolysis, granulocytic myeloid-derived suppressor cell (gMDSC), hypoxia, monocytic MDSC (mMDSC), MYC, proliferation, RAS, stroma/epithelial-to-mesenchymal transition/transforming growth factor-β, WNT). …”
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