Antitumor effects of zerumbone on rat hepatocarcinoma and HEPG2 cells by Taha, Manal Mohamed Elhassan

“…The liver sections of untreated DEN/ AAF rats also showed abundant PCNA, while in ZER-trcated rats the expression of this antigen was significantly (P<O.05) lowered. Formalin-fixed paraffin-embedded liver sections of untreated DEN/AAF rats also showed abundant AFP immunocxpression, while in ZER-treated rats the expression of this protein was significantly (P<O.05) lowered. …”
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Diversity and distribution of chaetognaths of different marine environments along the Straits of Malacca by De Guzman, Heidi Jane

“…Samples for biomass and taxonomic analyses were preserved in buffered formalin at 5% concentration. Chaeotognaths enumeration and identification were done under dissecting microscope. …”
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Development of galts and role of M cells towards feed-based vaccine against Streptococcus iniae infection in red hybrid tilapia (Oreochromis sp.) by Hayat, Mohammad

“…This study was conducted to determine the IgM antibodies, development of gutassociated lymphoid tissues (GALTs) and M cell’s role due to streptococcosis in red hybrid tilapia following oral administration of feed based formalin killed vaccine (FKV) of S. iniae and challenged with live S. iniae. …”
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Immunohistochemistry-derived subtypes of breast cancer distribution in four regions of Ethiopia by Esmael Besufikad Belachew, Esmael Besufikad Belachew, Esmael Besufikad Belachew, Adey Feleke Desta, Tewodros Yalew Gebremariam, Dinikisira Bekele Deneke, Senait Ashenafi, Melisachew Mulatu Yeshi, Bizunesh Dires Fenta, Alemwosen T/Hayimanot Alem, Addisu Alemu, Abdo Kedir Abafogi, Tigist Desta, Menberework Chanyalew, Daniel Beshah, Lesley Taylor, Marcus Bauer, Marcus Bauer, Dareskedar Tsehay, Selfu Girma, Daniel Seifu Melka, Tesfaye Sisay Tessema, Eva J. Kantelhardt, Eva J. Kantelhardt, Rawleigh Howe

“…This study was carried out to evaluate the distribution of immunohistochemistry (IHC) subtypes of BCs throughout four Ethiopian regions.MethodsA total of 227 formalin-fixed paraffin-embedded (FFPE) tissue blocks were collected from tertiary hospitals in four Ethiopian regions between 2015 and 2021. …”
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Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies by Ricardo Insausti, Ana María Insausti, Mónica Muñoz López, Isidro Medina Lorenzo, Maria del Mar Arroyo-Jiménez, María Pilar Marcos Rabal, Carlos de la Rosa-Prieto, José Carlos Delgado-González, Javier Montón Etxeberria, Sandra Cebada-Sánchez, Juan Francisco Raspeño-García, María Mercedes Iñiguez de Onzoño, Francisco Javier Molina Romero, Ruth Benavides-Piccione, Silvia Tapia-González, Laura E. M. Wisse, Sadhana Ravikumar, David A. Wolk, Javier DeFelipe, Paul Yushkevich, Emilio Artacho-Pérula

“…The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. …”
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Laboratory Cross-Comparison and Ring Test Trial for Tumor <i>BRCA</i> Testing in a Multicenter Epithelial Ovarian Cancer Series: The BORNEO GEICO 60-0 Study by Zaida Garcia-Casado, Ana Oaknin, Marta Mendiola, Gorka Alkorta-Aranburu, Jose Ramon Antunez-Lopez, Gema Moreno-Bueno, Jose Palacios, Alfonso Yubero, Raul Marquez, Alejandro Gallego, Ana Beatriz Sanchez-Heras, Jose Antonio Lopez-Guerrero, Cristina Perez-Segura, Pilar Barretina-Ginesta, Jesus Alarcon, Lydia Gaba, Antonia Marquez, Judit Matito, Juan Cueva, Isabel Palacio, Maria Iglesias, Angels Arcusa, Luisa Sanchez-Lorenzo, Eva Guerra-Alia, Ignacio Romero, Ana Vivancos

“…The study consisted of two independent experimental approaches, a bilateral comparison between two reference laboratories (RLs) testing 82 formalin-paraffin-embedded (FFPE) EOC samples each, and a Ring Test Trial (RTT) with five participating clinical laboratories (CLs) evaluating the performance of tissue <i>BRCA</i> testing in a total of nine samples. …”
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Gene variation profile and it’s potential correlation with clinical characteristics in HBV-associated HCC patients of Sichuan Han nationality in China by Jian Xu, Yao Zhou, Ke Dong, Jun Gong, Wei Xiong, Xu Wang, Chun Gu, Xiang-yu Lu, De-pei Huang, Xu-dong Shen, Xue-ke She, Xiao-chen Zhao, Xiao-jiong Yu, Hao Zhang

“…Whole exome sequencing and bioinformatics analysis were performed on formalin-fixed and paraffin-embedded samples from HCC. …”
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Zoobenthos in watercourses and water bodies in the Bolonsky State Nature Reserve (Russia) by Nadezhda M. Yavorskaya

“…All sampled were fixed in a 4% formalin solution. Amphibiotic insect adults were collected using an entomological net and light traps. …”
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Mutations in circulating tumor DNA detected in the postoperative period predict poor survival in patients with ovarian cancer by Angel Chao, Shu-Jen Chen, Hua-Chien Chen, Kien Thiam Tan, Wen Hsiao, Shih-Ming Jung, Lan-Yan Yang, Kuan-Gen Huang, Hung-Hsueh Chou, Huei-Jean Huang, Ting-Chang Chang, An-Shine Chao, Yun-Hsien Lee, Ren-Chin Wu, Chyong-Huey Lai

“…We also examined the concordance of mutations detected in ctDNA samples with those identified in the corresponding formalin-fixed paraffin-embedded (FFPE) tumor specimens. …”
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Exploring the impact of seasonal variations on the chemical composition, antinociceptive, and anti-inflammatory properties of Pogostemon heyneanus Benth. essential oil by Paulo Vinicius Lima Santos, Paulo Vinicius Lima Santos, Lucas Botelho Jerônimo, Weyda Suyane Campos Ribeiro, Gustavo Moraes Lopes, João Henrique de Castro Leão Neto, Hugo Borges Oliveira da Silva, Pedro Iuri C. da Silva, Pedro Iuri C. da Silva, Renata Cunha Silva, Joyce Kelly da Silva, Jofre Jacob S. Freitas, Rosa Helena V. Mourão, William N. Setzer, José Guilherme S. Maia, Pablo Luis B. Figueiredo, Pablo Luis B. Figueiredo

“…Neurogenic and inflammatory pain were evaluated by formalin test, and acute oral toxicity of the oil was also verified.Results: PhEO presented 27 chemical constituents with the highest predominance of patchoulol (43.6%–76.9%), α-bulnesene (0.2%–12.7%), α-guaiene (0.4%–8.9%), seychellene (3.8%–5.1%) and pogostol (0.0%–8.2%). …”
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The Effect of Chemical Buffering and Microbial Additives on Histomorphometry and Histopathology of the Small and Large Intestine of Lambs Fed High Concentrate Diets by Fereshteh Vafaee, Morteza Chaji, Omid Khorasani, Farshad Baghban

“…Each sample was placed separately in closed-sealed containers containing 10% formalin, and tissue changes were examined microscopically.Results and Discussion: No significant difference was observed between the experimental treatments for the tissue indices studied in the duodenum and jejunum. …”
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Construction of a risk stratification model integrating ctDNA to predict response and survival in neoadjuvant-treated breast cancer by Zhaoyun Liu, Bo Yu, Mu Su, Chenxi Yuan, Cuicui Liu, Xinzhao Wang, Xiang Song, Chao Li, Fukai Wang, Jianli Ma, Meng Wu, Dawei Chen, Jinming Yu, Zhiyong Yu

“…Expression profiling using a 457 cancer-related gene sequencing panel (DNA sequencing) covering genes recurrently mutated in breast cancer was carried out on 243 formalin-fixed paraffin-embedded tumor biopsies samples before NAC from 243 patients. …”
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Prediction of Response to Cisplatin-Based Neoadjuvant Chemotherapy of Muscle-Invasive Bladder Cancer Patients by Molecular Subtyping including KRT and FGFR Target Gene Assessment by Thorsten H. Ecke, Paula Carolin Voß, Thorsten Schlomm, Anja Rabien, Frank Friedersdorff, Dimitri Barski, Thomas Otto, Michael Waldner, Elke Veltrup, Friederike Linden, Roland Hake, Sebastian Eidt, Jenny Roggisch, Axel Heidenreich, Constantin Rieger, Lucas Kastner, Steffen Hallmann, Stefan Koch, Ralph M. Wirtz

“…The objective of the present study was to evaluate whether preoperative assessment of molecular subtype as well as FGFR target gene expression is predictive for therapeutic outcome—rate of ypT0 status—to justify subsequent prospective validation within the “BladderBRIDGister”. Formalin-fixed paraffin-embedded (FFPE) tissue specimens from transurethral bladder tumor resections (TUR) prior to neoadjuvant chemotherapy and corresponding radical cystectomy samples after chemotherapy of 36 patients were retrospectively collected. …”
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Genomic characterization reveals distinct mutation landscapes and therapeutic implications in neuroendocrine carcinomas of the gastrointestinal tract by Huanwen Wu, Zicheng Yu, Yueping Liu, Lei Guo, Lianghong Teng, Lingchuan Guo, Li Liang, Jing Wang, Jie Gao, Ruiyu Li, Ling Yang, Xiu Nie, Dan Su, Zhiyong Liang

“…Methods Comprehensive genomic analysis using whole‐exome sequencing was performed on 143 formalin‐fixed, paraffin‐embedded samples of surgically resected GI‐NEC with a thorough histological evaluation. …”
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Correlation Between IFNγ+ and CTLA-4+ Tumor Infiltrating Lymphocytes In Luminal And Non-Luminal Breast Carcinoma by Irene Lingkan Parengkuan, Sjahjenny Mustokoweni, Nila Kurniasari

“…Observational research with a cross-sectional approach was conducted on 40 formalin fixed paraffin-embedded tissue from breast carcinoma at the Anatomical Pathology Laboratory of Dr. …”
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Molluscan assemblage from a tropical intertidal estuarine sand-mud flat, Gulf of Nicoya, Pacific, Costa Rica (1984-1987) by José A Vargas-Zamora, Jeffrey A Sibaja-Cordero

“…Samples were preserved with 5% buffered formalin in sea water stained with Rose Bengal, and washed after 24 hours on a 500 micron mesh sieve. …”
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Pancreatic Cyst Size Measurement on Magnetic Resonance Imaging Compared to Pathology by Daniel Jeong, Brian Morse, Stuart Lane Polk, Dung-Tsa Chen, Jiannong Li, Pamela Hodul, Barbara A. Centeno, James Costello, Kun Jiang, Sebastian Machado, Issam El Naqa, Paola T. Farah, Tri Huynh, Natarajan Raghunand, Shaffer Mok, Aamir Dam, Mokenge Malafa, Aliya Qayyum, Jason B. Fleming, Jennifer B. Permuth

“…CONCLUSION: The maximum MRI IPMN size strongly correlated with but tended to overestimate the length compared to the pathology, potentially related to formalin tissue shrinkage during tissue processing.…”
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MCM-2 Levels as a Potential Biomarker for Predicting High-Risk Breast Cancer Patients According to TAILORx Classification by Ünal Ç, Özmen T, İlgün AS, Ordu Ç, Özkurt E, Ak N, Alço G, Erdoğan İyigün Z, Kurt S, Duymaz T, Öztürk MA, Elbüken Çelebi F, Yararbaş K, Soybir G, Aktepe F, Özmen V

“…According to the TAILORx trial, patients were divided into two groups with high (ODX-RS ≥ 26) and low risk (ODX-RS < 26) in terms of ODX-RS. Formalin-fixed-paraffin-embedded tissues of patients were re-evaluated, and 3 μm sections were prepared for MCM-2 immuno-histochemical staining. …”
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Quantitative multiplex immunohistochemistry reveals inter-patient lymphovascular and immune heterogeneity in primary cutaneous melanoma by Julia Femel, Cameron Hill, Irineu Illa Bochaca, Jamie L. Booth, Tina G. Asnaashari, Maria M. Steele, Ata S. Moshiri, Hyungrok Do, Judy Zhong, Judy Zhong, Iman Osman, Iman Osman, Sancy A. Leachman, Sancy A. Leachman, Takahiro Tsujikawa, Takahiro Tsujikawa, Kevin P. White, Young H. Chang, Young H. Chang, Amanda W. Lund, Amanda W. Lund, Amanda W. Lund, Amanda W. Lund, Amanda W. Lund, Amanda W. Lund, Amanda W. Lund

“…MethodsWe established a quantitative, multiplexed imaging platform to simultaneously detect immune infiltrates and tumor-associated vessels in formalin-fixed paraffin embedded patient samples. We performed a discovery, retrospective analysis of 28 treatment-naïve, primary cutaneous melanomas. …”
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An Italian Multicenter Perspective Harmonization Trial for the Assessment of MET Exon 14 Skipping Mutations in Standard Reference Samples by Paolo Bironzo, Francesco Pepe, Gianluca Russo, Pasquale Pisapia, Gianluca Gragnano, Gabriella Aquino, Silvia Bessi, Simonetta Buglioni, Federico Bartoccini, Giuseppina Ferrero, Michela Anna Bresciani, Paola Francia di Celle, Francesca Sibona, Andrea Giusti, Alessandra Movilia, Renata Mariella Farioli, Alessandra Santoro, Domenico Salemi, Stefania Scarpino, Dino Galafate, Stefania Tommasi, Rosanna Lacalamita, Davide Seminati, Elham Sajjadi, Silvia Novello, Fabio Pagni, Giancarlo Troncone, Umberto Malapelle

“…In this retrospective study, each institution received a set (<i>n</i> = 10) of a customized artificial formalin-fixed paraffin-embedded (FFPE) cell line (Custom <i>MET</i>ex14 skipping FFPE block) that harbored the <i>MET</i> exon 14 skipping mutation (Seracare Life Sciences, Milford, MA, USA), which was previously validated by the Predictive Molecular Pathology Laboratory at the University of Naples Federico II. …”
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