Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans by Giulio Valperga, Mario de Bono

“…We probe such mechanisms by performing a forward genetic screen for mutants with enhanced O2 perception in Caenorhabditis elegans. …”
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Mutation load decreases with haplotype age in wild Soay sheep by Martin A. Stoffel, Susan E. Johnston, Jill G. Pilkington, Josephine M. Pemberton

“…A 1% genomic increase in long ROH (>12.5 cM) reduced the odds of first‐year survival by 12.4% compared to only 7.7% for medium ROH (1.56–12.5 cM), whereas short ROH (<1.56 cM) had no effect on survival. We show by forward genetic simulations that this is predicted: compared to shorter ROH, long ROH will have higher densities of deleterious alleles, with larger average effects on fitness and lower population frequencies. …”
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ER proteostasis regulators cell-non-autonomously control sleep by Taizo Kawano, Mitsuaki Kashiwagi, Mika Kanuka, Chung-Kuan Chen, Shinnosuke Yasugaki, Sena Hatori, Shinichi Miyazaki, Kaeko Tanaka, Hidetoshi Fujita, Toshiro Nakajima, Masashi Yanagisawa, Yoshimi Nakagawa, Yu Hayashi

“…The molecular pathways in peripheral cells that trigger systemic sleep-related signals, however, are unclear. Here, a forward genetic screen in C. elegans identifies 3 genes that strongly affect sleep amount: sel-1, sel-11, and mars-1. sel-1 and sel-11 encode endoplasmic reticulum (ER)-associated degradation components, whereas mars-1 encodes methionyl-tRNA synthetase. …”
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The Sequencing-Based Mapping Method for Effectively Cloning Plant Mutated Genes by Li Yu, Yanshen Nie, Jinxia Jiao, Liufang Jian, Jie Zhao

“…A forward genetic approach is a powerful tool for identifying the genes underlying the phenotypes of interest. …”
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Mutations in dock1 disrupt early Schwann cell development by Rebecca L. Cunningham, Amy L. Herbert, Breanne L. Harty, Sarah D. Ackerman, Kelly R. Monk

“…Methods Through a forward genetic screen in zebrafish, we identified a mutation in the atypical guanine nucleotide exchange factor, dock1, that results in decreased myelination of peripheral axons. …”
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Genotype to Phenotype: CRISPR Gene Editing Reveals Genetic Compensation as a Mechanism for Phenotypic Disjunction of Morphants and Mutants by Cristy M. Salanga, Matthew C. Salanga

“…Forward genetic screens have shown the consequences of deleterious mutations; however, they are best suited for model organisms with fast reproductive rates and large broods. …”
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BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa by Tongbing Su, Weihong Wang, Zheng Wang, Peirong Li, Xiaoyun Xin, Yangjun Yu, Deshuang Zhang, Xiuyun Zhao, Jiao Wang, Liling Sun, Guihua Jin, Fenglan Zhang, Shuancang Yu

“…However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. …”
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Real-Time Monitoring and Analysis of Zebrafish Electrocardiogram with Anomaly Detection by Michael Lenning, Joseph Fortunato, Tai Le, Isaac Clark, Ang Sherpa, Soyeon Yi, Peter Hofsteen, Geethapriya Thamilarasu, Jingchun Yang, Xiaolei Xu, Huy-Dung Han, Tzung K. Hsiai, Hung Cao

“…Zebrafish (Danio rerio) are an excellent model system for studying heart disease as they enable a forward genetic approach to tackle this unmet medical need. …”
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Transcellular chaperone signaling is an intercellular stress-response distinct from the HSF-1-mediated heat shock response. by Jay Miles, Sarah Townend, Dovilė Milonaitytė, William Smith, Francesca Hodge, David R Westhead, Patricija van Oosten-Hawle

“…Here, we conducted a combinatorial approach using transcriptome RNA-Seq profiling and a forward genetic mutagenesis screen to elucidate how stress signaling from the intestine to the muscle is regulated. …”
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The RNA-binding protein Adad1 is necessary for germ cell maintenance and meiosis in zebrafish. by Kazi Nazrul Islam, Anuoluwapo Ajao, Kavita Venkataramani, Joshua Rivera, Shailja Pathania, Katrin Henke, Kellee Renee Siegfried

“…Here, through an N-ethyl-N-nitrosourea (ENU) based forward genetic screen, we identified an adad1 mutant zebrafish line that develops as sterile males. …”
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The Role of Transposable Element or Jumping Genes in Cancers by Abolfazl Movafagh

“…Performing an unbiased forward genetic screen in human provides the tools to generate tumors and analyze their genetic composition, while reducing the background of passenger mutations. …”
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Characterization of the innate immune response to Streptococcus pneumoniae infection in zebrafish. by Anni K Saralahti, Sanna-Kaisa E Harjula, Tommi Rantapero, Meri I E Uusi-Mäkelä, Mikko Kaasinen, Maiju Junno, Hannaleena Piippo, Matti Nykter, Olli Lohi, Samuli Rounioja, Mataleena Parikka, Mika Rämet

“…In addition, to gain understanding of the genetic factors associated with the increased risk for severe pneumococcal infection in humans, we carried out a medium-scale forward genetic screen in zebrafish. In the screen, we identified a mutant fish line which showed compromised resistance to pneumococcus in the septic larval infection model. …”
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Thermal and area optimization for component placement on PCB design using inverse genetic algorithm by Abubakar, Abubakar Kamal

“…In the conventional Forward Genetic Algorithm (FGA) optimization, the individual fitness of components are generated through the GA process. …”
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An intestinally secreted host factor promotes microsporidia invasion of C. elegans by Hala Tamim El Jarkass, Calvin Mok, Michael R Schertzberg, Andrew G Fraser, Emily R Troemel, Aaron W Reinke

“…To identify such factors necessary for infection by Nematocida parisii, a natural microsporidian pathogen of Caenorhabditis elegans, we performed a forward genetic screen to identify mutant animals that have a Fitness Advantage with Nematocida (Fawn). …”
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The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis by Jin-Yu Li, Jing-Liang Sun, Ying-Ying Tian, Jian-Xiang Liu

“…Here, a temperature-conditioned chloroplast development defective mutant thermo-sensitive mutant in leaf color 2 (tsl2) of Arabidopsis was obtained through a forward genetic screening. The tsl2 mutant showed a weak yellowish phenotype at normal growth temperature (22°C), and the phenotype was more pronounced at low growth temperature (16°C) and largely rescued at high growth temperature (29°C). …”
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Chlamydia trachomatis’ struggle to keep its host alive by Barbara S. Sixt, Raphael H. Valdivia, Guido Kroemer

“…We recently conducted a forward genetic screen in Chlamydia trachomatis, a common sexually transmitted human pathogen, and identified a mutant that caused premature death in the majority of infected host cells. …”
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Functional Whole Genome Screen of Nutrient-Starved <i>Mycobacterium tuberculosis</i> Identifies Genes Involved in Rifampin Tolerance by William M. Matern, Harley T. Harris, Carina Danchik, Marissa McDonald, Gopi Patel, Aashish Srivastava, Thomas R. Ioerger, Joel S. Bader, Petros C. Karakousis

“…An improved understanding of the mechanisms mediating antibiotic tolerance in <i>Mtb</i> can serve as the basis for developing more effective therapies. We performed a forward genetic screen to identify candidate <i>Mtb</i> genes involved in tolerance to the two key first-line antibiotics, rifampin and isoniazid, under nutrient-rich and nutrient-starved conditions. …”
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Cloning and Functional Analysis of Dwarf Gene <i>Mini Plant 1</i> (<i>MNP1</i>) in <i>Medicago truncatula</i> by Shiqi Guo, Xiaojia Zhang, Quanzi Bai, Weiyue Zhao, Yuegenwang Fang, Shaoli Zhou, Baolin Zhao, Liangliang He, Jianghua Chen

“…Our study found that both cell length and the cell number of internodes were reduced in a <i>mnp1</i> mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned the <i>MNP1</i> gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. …”
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A high-resolution genotype–phenotype map identifies the TaSPL17 controlling grain number and size in wheat by Yangyang Liu, Jun Chen, Changbin Yin, Ziying Wang, He Wu, Kuocheng Shen, Zhiliang Zhang, Lipeng Kang, Song Xu, Aoyue Bi, Xuebo Zhao, Daxing Xu, Zhonghu He, Xueyong Zhang, Chenyang Hao, Jianhui Wu, Yan Gong, Xuchang Yu, Zhiwen Sun, Botao Ye, Danni Liu, Lili Zhang, Liping Shen, Yuanfeng Hao, Youzhi Ma, Fei Lu, Zifeng Guo

“…Conclusion Our study provides valuable resources for genetic improvement of spike morphology and a fast-forward genetic solution for candidate gene detection and cloning in wheat.…”
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Functional Whole Genome Screen of Nutrient-Starved Mycobacterium tuberculosis Identifies Genes Involved in Rifampin Tolerance by Matern, William M., Harris, Harley T., Danchik, Carina, McDonald, Marissa, Patel, Gopi, Srivastava, Aashish, Ioerger, Thomas R., Bader, Joel S., Karakousis, Petros C.

“…An improved understanding of the mechanisms mediating antibiotic tolerance in <i>Mtb</i> can serve as the basis for developing more effective therapies. We performed a forward genetic screen to identify candidate <i>Mtb</i> genes involved in tolerance to the two key first-line antibiotics, rifampin and isoniazid, under nutrient-rich and nutrient-starved conditions. …”
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