cacna2d3, a voltage-gated calcium channel subunit, functions in vertebrate habituation learning and the startle sensitivity threshold. by Nicholas J Santistevan, Jessica C Nelson, Elelbin A Ortiz, Andrew H Miller, Dima Kenj Halabi, Zoë A Sippl, Michael Granato, Yevgenya Grinblat

“…<h4>Methods</h4>To identify genes critical for plasticity, a forward genetic screen for zebrafish genes that mediate habituation learning was performed, which identified a mutant allele, doryp177, that caused reduced habituation of the acoustic startle response. …”
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Plexin A3 and turnout regulate motor axonal branch morphogenesis in zebrafish. by Rajiv Sainath, Michael Granato

“…This precisely timed and spatially restricted branching process is disrupted in turnout mutants we identified in a forward genetic screen. Molecular genetic mapping positioned the turnout mutation within a 300 kb region encompassing eight annotated genes, however sequence analysis of all eight open reading frames failed to unambiguously identify the turnout mutation. …”
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Opposing action of the FLR-2 glycoprotein hormone and DRL-1/FLR-4 MAP kinases balance p38-mediated growth and lipid homeostasis in C. elegans. by Sarah K Torzone, Aaron Y Park, Peter C Breen, Natalie R Cohen, Robert H Dowen

“…To identify factors that oppose DRL-1/FLR-4, we performed a forward genetic screen for suppressors of the drl-1 mutant phenotypes and identified mutations in flr-2 and fshr-1, which encode the orthologues of follicle stimulating hormone and its putative G protein-coupled receptor, respectively. …”
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Potent, specific MEPicides for treatment of zoonotic staphylococci. by Rachel L Edwards, Isabel Heueck, Soon Goo Lee, Ishaan T Shah, Justin J Miller, Andrew J Jezewski, Marwa O Mikati, Xu Wang, Robert C Brothers, Kenneth M Heidel, Damon M Osbourn, Carey-Ann D Burnham, Sophie Alvarez, Stephanie A Fritz, Cynthia S Dowd, Joseph M Jez, Audrey R Odom John

“…In addition, we have both enzymatically and structurally determined the mechanism by which FSM elicits its effect. Using a forward genetic screen, the glycerol-3-phosphate transporter GlpT that facilitates FSM uptake was identified in two zoonotic staphylococci, Staphylococcus schleiferi and Staphylococcus pseudintermedius. …”
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Mate selection: A useful approach to maximize genetic gain and control inbreeding in genomic and conventional oil palm (Elaeis guineensis Jacq.) hybrid breeding. by Billy Tchounke, Leopoldo Sanchez, Joseph Martin Bell, David Cros

“…Here, we investigated this aspect with forward genetic simulations on a high-performance computing cluster and massively parallel computing, considering the oil palm hybrid breeding example. …”
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A <i>sart1</i> Zebrafish Mutant Results in Developmental Defects in the Central Nervous System by Hannah E. Henson, Michael R. Taylor

“…The complex then associates with U1 and U2 snRNPs to form the spliceosome. A forward genetic screen identifying defects in choroid plexus development and whole-exome sequencing (WES) identified a point mutation in exon 12 of <i>sart1</i> in <i>Danio rerio</i> (zebrafish). …”
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N-Acetyltransferase 9 ameliorates Aβ42-mediated neurodegeneration in the Drosophila eye by Prajakta Deshpande, Anuradha Venkatakrishnan Chimata, Emily Snider, Aditi Singh, Madhuri Kango-Singh, Amit Singh

“…To address this gap in information, we conducted a forward genetic screen, and identified N-acetyltransferase 9 (Mnat9) as a genetic modifier of GMR > Aβ42 neurodegenerative phenotype. …”
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Modulation of host NF-[k̳̳a̳p̳p̳a̳]B pathway by the Toxoplasma gondii secreted factor, GRA15 by Lu, Diana, Ph. D. Massachusetts Institute of Technology

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Functional genomics and compound mode-of-action screening in haploid human cells by Gapp, B

“…The third chapter presents a pilot study describing the set-up of a forward genetic technique for compound mode-of-action screening using a pooled human mutant cell line collection. …”

Investigating the roles of zinc finger homeobox 3 in circadian rhythms by Edwards, JK

“…This exemplifies that molecular components of the circadian oscillator may remain to be identified and forward genetic approaches are facilitating advancements in this field.…”

The genetics of autoimmune and proteinuric disease by Bull, K

“…The objective of this thesis is to identify rare variants of relevance to autoimmune and renal disease, by developing ways of analysing whole genome sequencing (WGS) data and exploring the variants identified. Forward genetic experimental approaches are used, both in mutagenised mice, and in humans with extreme trait forms of the steroid resistant nephrotic syndrome (SRNS) and systemic lupus erythematosus (SLE). …”

The <i>Drosophila</i> Mutagen-Sensitivity Gene <i>mus109</i> Encodes <i>DmDNA2</i> by Chandani Mitchell, Vada Becker, Jordan DeLoach, Erica Nestore, Elyse Bolterstein, Kathryn P. Kohl

“…The identification of mutants through forward genetic screens is the backbone of <i>Drosophila</i> genetics research, yet many mutants identified through these screens have yet to be mapped to the <i>Drosophila</i> genome. …”
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UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean by Jiaqi Sun, Shiyu Huang, Qing Lu, Shuo Li, Shizhen Zhao, Xiaojian Zheng, Qian Zhou, Wenxiao Zhang, Jie Li, Lili Wang, Ke Zhang, Wenyu Zheng, Xianzhong Feng, Baohui Liu, Fanjiang Kong, Fengning Xiang

“…It has not been reported that the E3 ubiquitin ligase Anaphase Promoting Complex/Cyclosome (APC/C) is involved in the regulation of plant growth in response to environmental factors. We perform a forward genetic screen in soybean and find that a mutation in Glycine max Increased Leaf Petiole Angle1 (GmILPA1), encoding a subunit of the APC/C, lead to dwarfism under UV-B irradiation. …”
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New Model Systems and the Development of Targeted Therapies for the Treatment of Neurofibromatosis Type 1-Associated Malignant Peripheral Nerve Sheath Tumors by Kyle B. Williams, David A. Largaespada

“…These have come from studies of RAS biochemistry, in vitro drug screening, forward genetic screens for Schwann cell tumors, and synthetic lethal screens in cells with oncogenic <i>RAS</i> gene mutations. …”
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A forward phenotypically driven unbiased genetic analysis of host genes that moderate herpes simplex virus virulence and stromal keratitis in mice. by Richard L Thompson, Robert W Williams, Malak Kotb, Nancy M Sawtell

“…To identify such host gene variants we have initiated a forward genetic analysis using the expanded family of BXD strains, all derived from crosses between C57BL/6J and DBA/2J strains of mice. …”
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Critical role for uricase and xanthine dehydrogenase in soybean nitrogen fixation and nodule development by Cuong X. Nguyen, Alice Dohnalkova, C. Nathan Hancock, Kendall R. Kirk, Gary Stacey, Minviluz G. Stacey

“…While efforts to develop large mutant collections of soybean are underway, research on this plant is still hampered by the inability to obtain mutations in any specific gene of interest. Using a forward genetic approach, as well as CRISPR/Cas9 gene editing via Agrobacterium rhizogenes‐mediated hairy root transformation, we identified and characterized the role of GmUOX (Uricase) and GmXDH (Xanthine Dehydrogenase) in nitrogen fixation and nodule development in soybean. …”
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Targeting prolyl-tRNA synthetase via a series of ATP-mimetics to accelerate drug discovery against toxoplasmosis by Manickam Yogavel, Alexandre Bougdour, Siddhartha Mishra, Nipun Malhotra, Jyoti Chhibber-Goel, Valeria Bellini, Karl Harlos, Benoît Laleu, Mohamed-Ali Hakimi, Amit Sharma

“…Cell-based chemical mutagenesis was employed to determine the mechanism of action via a forward genetic screen. Tg-resistant parasites were analyzed with wild-type strain by RNA-seq to identify mutations in the coding sequence conferring drug resistance by computational analysis of variants. …”
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Whole Genome Sequencing and a New Bioinformatics Platform Allow for Rapid Gene Identification in D. melanogaster EMS Screens by Jeannette Osterloh, Mary Logan, Marc Freeman, Rafael F. Acosta Lebrigio, Rick H. Ulloa, Derek Van Booven, William Hulme, Michael A. Gonzalez, Stephan Zuchner

“…Forward genetic screens in Drosophila melanogaster using ethyl methanesulfonate (EMS) mutagenesis are a powerful approach for identifying genes that modulate specific biological processes in an in vivo setting. …”
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Genetic analysis of endoplasmic reticulum homeostasis during stress and infection of Caenorhabditis elegans by Tillman, Erik J. (Erik James)

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Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development by Ricardo A. DeMoya, Rachel E. Forman-Rubinsky, Deon Fontaine, Joseph Shin, Simon C. Watkins, Cecilia W. Lo, Michael Tsang

“…Hypoplastic left heart syndrome (HLHS) is a congenital heart disease where the left ventricle is reduced in size. A forward genetic screen in mice identified SIN3A associated protein 130 kDa (Sap130), part of the chromatin modifying SIN3A/HDAC complex, as a gene contributing to the etiology of HLHS. …”
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