Flavivirus–Host Interaction Landscape Visualized through Genome-Wide CRISPR Screens by Aditi Kanojia, Mansi Sharma, Rishad Shiraz, Shashank Tripathi

“…Clustered regularly interspaced short palindromic repeats (CRISPR) technology has provided an effective means of producing customizable genetic modifications and performing forward genetic screens in a broad spectrum of cell types and organisms. …”
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<i>Ss</i>Cak1 Regulates Growth and Pathogenicity in <i>Sclerotinia sclerotiorum</i> by Lei Qin, Jieying Nong, Kan Cui, Xianyu Tang, Xin Gong, Yunong Xia, Yan Xu, Yilan Qiu, Xin Li, Shitou Xia

“…It is of vital importance to understand its pathogenic mechanism for disease control. Through a forward genetic screen combined with next-generation sequencing, a putative protein kinase, <i>Ss</i>Cak1, was found to be involved in the growth and pathogenicity of <i>S. sclerotiorum</i>. …”
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NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals by Dane Kawano, Katherine Pinter, Madison Chlebowski, Ronald S. Petralia, Ya-Xian Wang, Alex V. Nechiporuk, Catherine M. Drerup

“…Here, we report an unexplored function of dynein in microtubule regulation in axon terminals: regulation of microtubule stability. Using a forward genetic screen, we identified a mutant with an abnormal axon terminal structure owing to a loss of function mutation in NudC. …”
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MEGF8 is a modifier of BMP signaling in trigeminal sensory neurons by Caitlin Engelhard, Sarah Sarsfield, Janna Merte, Qiang Wang, Peng Li, Hideyuki Beppu, Alex L Kolodkin, Henry M Sucov, David D Ginty

“…Using a three-generation forward genetic screen in mice we have identified Megf8 as a novel modifier of BMP4 signaling in trigeminal ganglion (TG) neurons. …”
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Lipid transfer from plants to arbuscular mycorrhiza fungi by Andreas Keymer, Priya Pimprikar, Vera Wewer, Claudia Huber, Mathias Brands, Simone L Bucerius, Pierre-Marc Delaux, Verena Klingl, Edda von Röpenack-Lahaye, Trevor L Wang, Wolfgang Eisenreich, Peter Dörmann, Martin Parniske, Caroline Gutjahr

“…However, de novo fatty acid (FA) synthesis has not been observed in AM fungi in absence of the plant. In a forward genetic approach, we identified two Lotus japonicus mutants defective in AM-specific paralogs of lipid biosynthesis genes (KASI and GPAT6). …”
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COMP-1 promotes competitive advantage of nematode sperm by Jody M Hansen, Daniela R Chavez, Gillian M Stanfield

“…In this study, we utilize a forward genetic screen in Caenorhabditis elegans to identify a gene, comp-1, whose function is specifically required in competitive contexts. …”
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HIV-1 infects macrophages by exploiting an endocytic route dependent on dynamin, Rac1 and Pak1. by Carter, G, Bernstone, L, Baskaran, D, James, W

“…Because of the limitations of a purely pharmacological study such as this, the final elucidation of this pathway awaits the development of reliable forward genetic approaches in authentic macrophages.…”

Deficiency of the HGF/Met pathway leads to thyroid dysgenesis by impeding late thyroid expansion by Ya Fang, Jia-Ping Wan, Zheng Wang, Shi-Yang Song, Cao-Xu Zhang, Liu Yang, Qian-Yue Zhang, Chen-Yan Yan, Feng-Yao Wu, Sang-Yu Lu, Feng Sun, Bing Han, Shuang-Xia Zhao, Mei Dong, Huai-Dong Song

“…Here we find three zebrafish lines from a forward genetic screening with similar thyroid dysgenesis phenotypes and identify a stop-gain mutation in hgfa and two missense mutations in met by positional cloning from these zebrafish lines. …”
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Bacterial cell cycle control by citrate synthase independent of enzymatic activity by Matthieu Bergé, Julian Pezzatti, Víctor González-Ruiz, Laurence Degeorges, Geneviève Mottet-Osman, Serge Rudaz, Patrick H Viollier

“…How central energy metabolism regulates bacterial cell cycle functions is not well understood. Our forward genetic selection unearthed the Krebs cycle enzyme citrate synthase (CitA) as a checkpoint regulator controlling the G1→S transition in the polarized alpha-proteobacterium Caulobacter crescentus, a model for cell cycle regulation and asymmetric cell division. …”
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A role for glycolipid biosynthesis in severe fever with thrombocytopenia syndrome virus entry. by Mary Jane Drake, Benjamin Brennan, Kenneth Briley, Stephen M Bart, Eric Sherman, Agnieszka M Szemiel, Madeleine Minutillo, Frederic D Bushman, Paul Bates

“…Little is known about the viral entry requirements beyond showing dependence on dynamin and endosomal acidification. A haploid forward genetic screen was performed to identify host cell requirements for SFTSV entry. …”
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Clarinet (CLA-1), a novel active zone protein required for synaptic vesicle clustering and release by Zhao Xuan, Laura Manning, Jessica Nelson, Janet E Richmond, Daniel A Colón-Ramos, Kang Shen, Peri T Kurshan

“…Active zone proteins cluster synaptic vesicles at presynaptic terminals and coordinate their release. In forward genetic screens, we isolated a novel Caenorhabditis elegans active zone gene, clarinet (cla-1). cla-1 mutants exhibit defects in synaptic vesicle clustering, active zone structure and synapse number. …”
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Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62 by Rowena DeJesus, Francesca Moretti, Gregory McAllister, Zuncai Wang, Phil Bergman, Shanming Liu, Elizabeth Frias, John Alford, John S Reece-Hoyes, Alicia Lindeman, Jennifer Kelliher, Carsten Russ, Judith Knehr, Walter Carbone, Martin Beibel, Guglielmo Roma, Aylwin Ng, John A Tallarico, Jeffery A Porter, Ramnik J Xavier, Craig Mickanin, Leon O Murphy, Gregory R Hoffman, Beat Nyfeler

“…Here, we describe a forward genetic screening paradigm exploiting CRISPR-mediated genome editing coupled to a cell selection step by FACS to identify regulators of SQSTM1. …”
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Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo by Darryl A Wesener, Zachary W Beller, Samantha L Peters, Amir Rajabi, Gianluca Dimartino, Richard J Giannone, Robert L Hettich, Jeffrey I Gordon

“…To illustrate one approach, an arabinan-containing polysaccharide was isolated from pea fiber, its structure defined, and forward genetic and proteomic analyses used to compare its effects, versus unfractionated pea fiber and sugar beet arabinan, on a human gut bacterial strain consortium in gnotobiotic mice. …”
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CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors by Manuela D'Alessandro, Magali Richard, Christian Stigloher, Vincent Gache, Thomas Boulin, Janet E Richmond, Jean-Louis Bessereau

“…The assembly of neurotransmitter receptors in the endoplasmic reticulum limits the number of receptors delivered to the plasma membrane, ultimately controlling neurotransmitter sensitivity and synaptic transfer function. In a forward genetic screen conducted in the nematode C. elegans, we identified crld-1 as a gene required for the synaptic expression of ionotropic acetylcholine receptors (AChR). …”
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Identifying C. elegans lifespan mutants by screening for early-onset protein aggregation by Daniel F. Midkiff, Javier Huayta, James D. Lichty, Joseph P. Crapster, Adriana San-Miguel

“…In Caenorhabditis elegans, forward genetic screens rely on the isolation of reproductively active mutants that can self-propagate clonal populations. …”
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The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway by Jingmei Song, Jianlong Ma, Xing Liu, Zhuofu Huang, Lianghui Li, Linke Li, Lingfei Luo, Rui Ni, Jianbo He

“…Here, we perform an N-ethyl-N-nitrosourea (ENU) forward genetic screen and identify a mutant containing a nonsense mutation in the gene nibrin (nbn), which encodes a component of the Mre11-Rad50-Nbn (MRN) complex that activates DNA damage response (DDR). …”
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Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening by Joung, Julia, Konermann, Silvana M, Gootenberg, Jonathan S, Abudayyeh, Omar Osama, Platt, Randall Jeffrey, Brigham, Mark D, Sanjana, Neville E, Zhang, Feng

“…Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. …”
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The lta4h locus modulates susceptibility to mycobacterial infection in zebrafish and humans. by Tobin, D, Vary, J, Ray, J, Walsh, G, Dunstan, S, Bang, N, Hagge, D, Khadge, S, King, M, Hawn, T, Moens, C, Ramakrishnan, L

“…Here we report results from a forward genetic screen in zebrafish larvae that identify multiple mutant classes with distinct patterns of innate susceptibility to Mycobacterium marinum. …”

Identification of 34 novel proinflammatory proteins in a genome-wide macrophage functional screen. by Wyllie, D, Sogaard, K, Holland, K, Yaobo, X, Bregu, M, Hill, A, Kiss-Toth, E

“…In order to characterise novel regulators of macrophage inflammation, we have carried out an extensive, cDNA-based forward genetic screen and identified 34 novel activators, based on their ability to induce the expression of cxcl2. …”

A streamlined method for transposon mutagenesis of Rickettsia parkeri yields numerous mutations that impact infection. by Rebecca L Lamason, Natasha M Kafai, Matthew D Welch

“…Given the need to identify critical virulence factors, forward genetic screens such as this will provide an excellent platform to more directly investigate rickettsial biology and pathogenesis.…”
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