Genetic and Epigenetic Evaluation of Human Spermatogonial Stem Cells Isolated by MACS in Different Two and Three-Dimensional Culture Systems by Maria Zahiri, Mansoureh Movahedin, Seyed Javad Mowla, Mehrdad Noruzinia, Morteza Koruji, Mohammad Reza Nowroozi, Fatemeh Asgari

“…Gene-specific methylation by MSP, expression of pluripotency (NANOG, C-MYC and OCT-4), and germ cells specific genes (Integrin α6, Integrin β1, PLZF) evaluated. …”
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Integrated bioinformatics analysis of the transcription factor-mediated gene regulatory networks in the formation of spermatogonial stem cells by Kesong Shi, Baoluri Wang, Le Dou, Shu Wang, Xinrui Fu, Haiquan Yu

“…Then, we applied RNA-seq and microarray data screening for key SSC-specific TFs and constructed key SSC-specific TF-mediated gene regulatory networks (GRNs) using ChIP-seq data.Results: First, after analysis of the ATAC-seq and DNase-seq data of mouse ESCs, primordial germ cells (PGCs), and SSCs, 33 SSC-specific TFs and 958 targeting genes were obtained. …”
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Multiple circRNAs regulated by QKI5 conjointly spongemiR-214-3p to antagonize bisphenol A-inducedspermatocyte toxicity by Li Huimin, Zhao Yunhan, Shen Qiuzi, Li Honggang

“…Our previous study revealed that some up-regulated circRNAs play protective roles in bisphenol A (BPA)-induced toxicity in GC-2 germ cells. Here by CCK-8 assay, apoptosis assay, qRT-PCR and western blot analysis, we further discover that circRNAs (represented by circDcbld2, circMapk1 and circTbcld20) can cooperatively sponge miR-214-3p and then up-regulate AKT1 in ameliorating BPA-induced reproductive toxicity. …”
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Effects of ionizing radiation on the human body by Patrycja Ręba

“…In contrast, genetic effects manifest themselves mostly in the germ cells and cause changes in successive generations.And the second type of effects of ionizing radiation or deterministictic They occur when a person receives a relatively large doses, which can provide temporary or permanent tissue damage. …”
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Comparative assessment of reproductive capacity of boar-sires depending on the breed and season by O. S. Miroshnikova, S. V. Naumenko

“…According to the results of the annual analysis of the sperm production of boars, a high degree of culling of ejaculates was established for various reasons (high level of sperm agglutination (52.3–74.0 %), low concentration of germ cells (14.5–29.8 %), presence of foreign impurities (5,8–12.4 %). …”
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Serum and seminal plasma levels of interleukin-17 in infertile patients by Abdelrahman A. Elsaied, Hassan M. Ibrahim, Eisa M. Hegazy, Mariam Romany Wahip*

“…Background: Signal transduction induced by cytokines, which stimulates cell growth, proliferation, differentiation of germ cells, spermatogenesis, reproductive and testicular function. …”
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Functional interplay between ribosomal protein paralogues in the eRpL22 family in Drosophila melanogaster by Catherine M. Mageeney, Michael G. Kearse, Brett W. Gershman, Caroline E. Pritchard, Jennifer M. Colquhoun, Vassie C. Ware

“…Paralogue depletion unmasked mechanisms, yet to be defined that impact paralogue co-expression within germ cells.…”
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Cellular Therapy via Spermatogonial Stem Cells for Treating Impaired Spermatogenesis, Non-Obstructive Azoospermia by Nesma E. Abdelaal, Bereket Molla Tanga, Mai Abdelgawad, Sahar Allam, Mostafa Fathi, Islam M. Saadeldin, Seonggyu Bang, Jongki Cho

“…SSCs originate from gonocytes in the postnatal testis, which originate from long-lived primordial germ cells during embryonic development. The treatment of infertility in males has a poor prognosis. …”
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The Role of LIM Kinase in the Male Urogenital System by Juhyun Park, Soo Woong Kim, Min Chul Cho

“…Moreover, positive signals with LIMK2-specific antibodies were detected mainly in the nucleus of the differentiated stages of germ cells, such as spermatocytes and early round spermatids. …”
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A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis by Selin Önen, Ali Can Atik, Merve Gizer, Sevil Köse, Önder Yaman, Haluk Külah, Petek Korkusuz

“…The new setup improved in vitro spermatogenesis in terms of c-Kit(+) differentiating spermatogonia, VASA(+) total germ cells, the meiotic cells including spermatocytes and spermatids and testicular maturation by increasing testosterone concentration and improved tubular growth for 42 days in comparison with hanging drop and non-mesenchymal stem cell control. …”
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Phthalate plasticizer decreases the prion-like protein doppel essential for structural integrity and function of spermatozoa by Jae-Hyeon Lee, Seung Hyun Park, Chongsuk Ryou, Myung Chan Gye

“…Together, our findings suggest that DBP at present dosage may potentiate LPO generation and Sertoli cell immaturity via downregulation of Sox9 and disruption of the Pou4f1-Prnd gene network in post-meiotic germ cells without visible changes in spermatogenesis or testosterone level. …”
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Designing, cloning and simulation studies of cancer/testis antigens based multi-epitope vaccine candidates against cutaneous melanoma: An immunoinformatics approach by Sana Khalid, Jinlei Guo, Syed Aun Muhammad, Baogang Bai

“…The CTA family is a group of antigens that are only expressed in malignancies and testicular germ cells. Methods: We used integrative framework and systems-level analysis to predict potential vaccine candidates for cutaneous melanoma involving epitopes prediction, molecular modeling and molecular docking to cross-validate the binding affinity and interaction between potential vaccine agents and major histocompatibility molecules (MHCs) followed by molecular dynamics simulation, immune simulation and in silico cloning. …”
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Biological Effects of Magnetic Resonance Imaging on Testis Histology and Seminiferous Tubules Morphometry by Ayoob Rostamzadeh, Seyed Hadi Anjamrooz, Mohammad Jafar Rezaie, Fardin Fathi, Mohsen Mohammadi

“…Conclusions: Long-term exposure to MRI waves has deleterious effects on the male reproductive system, fertility parameters, and the quantity of germ cells in the seminiferous tubules with the exception of the number of round spermatid cells and epithelial thickness. …”
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Effect of Chemotherapy Cytarabine and Acute Myeloid Leukemia on the Development of Spermatogenesis at the Adult Age of Immature Treated Mice by Bara’ah Khaleel, Eitan Lunenfeld, Joseph Kapelushnik, Mahmoud Huleihel

“…Our result showed that AML and CYT affected the microenvironment/niche of the germ cells. AML significantly decreased the levels growth factors, such as SCF, GDNF and MCSF) compared to control, whereas CYT significantly increased the levels of MCSF and GDNF compared to control. …”
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Influence of the type of higher nervous activity on seminal physiological characteristics in cats of Russian breeds by Alina V. Petryaeva, Aleksandr V. Tkachev, Olga L. Tkacheva

“…Sperm motility after thawing was higher in cats with inert temperament, which was 5.06 % more (P 0.01) compared to cats with mobile temperament and weak nervous system and 9.1 % more (P 0.001) than in unbalanced cats. Survivability of germ cells in Russian cats at 38 C after defrosting was greatest in live sexual temperament cats, which was 0.22 hours more in comparison with calm sexual temperament cats, 0.62 hours more (P 0.01) than in cats with weak sexual temperament and 0.91 hours more than in cats with unrestrained sexual temperament.…”
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Intracellular delivery of radioimmunoconjugates that target the cancer testis antigen, NY-ESO-1 by Chu, H

“…<p>Cancer testis antigens (CTA) represent attractive targets for targeted radiotherapy and imaging as their expression is restricted to cancer and germ cells. NY-ESO-1, a member of the CTA family, is highly immunogenic and expressed in multiple tumor types including carcinoma of bladder, liver lung. …”

Identification of genes involved in urogenital development by Van Hateren, N

“…The indifferent gonad, or genital ridge, arises on the ventro-medial aspect of the mesonephros at approximately 10 days <em>post coitum</em> (dpc), and enlarges due to the influx of germ cells and migration of somatic cells from the coelomic epithelium and mesonephros. …”

Partial <i>fads2</i> Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Δ8 Pathway with an Impact on the Reproduction of Female Zebrafish (<i>Danio rerio</i>) by Zuzana Bláhová, Roman Franěk, Marek Let, Martin Bláha, Martin Pšenička, Jan Mráz

“…In anamniotic vertebrates, such as fish, there is still no information available on the reproductive (in)ability of <i>fads2</i> mutants, although zebrafish have become an increasingly important model of lipid metabolism, including some aspects of the generation of germ cells and early embryonic development. In the present study, we apply the CRISPR/Cas9 genome editing system to induce mutations in the zebrafish genome and create crispants displaying a degree of <i>fads2</i> gene editing within the range of 50–80%. …”
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<i>BAP1</i> Malignant Pleural Mesothelioma Mutations in <i>Caenorhabditis elegans</i> Reveal Synthetic Lethality between <i>ubh-4</i>/<i>BAP1</i> and the Proteasome Subunit <i>rpn-... by Carmen Martínez-Fernández, Sweta Jha, Elisabet Aliagas, Carina I. Holmberg, Ernest Nadal, Julián Cerón

“…<i>ubh-4</i>[A87D], similarly to <i>ubh-4</i> deletion, cause a synthetic genetic interaction with <i>rpn-9</i> inactivation affecting body size, lifespan, and the development of germ cells. Finally, we show how <i>ubh-4</i> inactivation sensitizes animals to the chemotherapeutic agent Bortezomib, which is a proteasome inhibitor. …”
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Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of the dnd1 gene by CRISPR/Cas9 genome editing by Wai-Kwan Chu, Wai-Kwan Chu, Shih-Chin Huang, Ching-Fong Chang, Ching-Fong Chang, Jen-Leih Wu, Jen-Leih Wu, Hong-Yi Gong, Hong-Yi Gong

“…Based on a comparison of histological sections of the gonads between wild-type, heterozygous, and homozygous dnd1 zebrafish mutants, the dnd1 homozygous mutation (aa) resulted in the loss of germ cells. Still, there was no difference between the wild-type (AA) and dnd1 heterozygous (Aa) zebrafish. …”
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