Looking beyond toxicities: Other health-related morbidities noted in childhood solid tumor survivors by Mehak Sehgal, Vishesh Jain, Sandeep Agarwala, Anjan Dhua, Prabudh Goel, Devendra Kumar Yadav, Sameer Bakhshi, Mani Kalaivani

“…Materials and Methods: The study included a cohort of hepatoblastoma (HB), Wilm's tumor (WT), and malignant germ cell tumors (MGCT) survivors registered at pediatric surgical-oncology clinic from 1994 to 2016. …”
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Mucinous cystadenocarcinoma of the ovary in a 14-year-old girl: a case report and literature review by Qiong Li, Cancan Zou, Yangyang Xu, Shiqing Liu, Tianjing Yan

“…In children, most ovarian tumors are germ cell-derived, whereas epithelial tumors are rare and mostly benign. …”
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The high diversity of gametogenic pathways in amphispermic water frog hybrids from Eastern Ukraine by Eleonora Pustovalova, Lukaš Choleva, Dmytro Shabanov, Dmitrij Dedukh

“…We found that the genome composition and ploidy of germ cells, meiotic cells, and spermatids vary among P. esculentus individuals. …”
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p53 regulates diverse tissue-specific outcomes to endogenous DNA damage in mice by Ross J. Hill, Nazareno Bona, Job Smink, Hannah K. Webb, Alastair Crisp, Juan I. Garaycoechea, Gerry P. Crossan

“…We show that ablation of p53 rescues the loss of hematopoietic stem cells, and has no effect on kidney, germ cell or brain dysfunction, but exacerbates liver pathology and polyploidisation. …”
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Effects of hyperthyroidism and diabetes mellitus on spermatogenesis in peri- and post-pubertal mice by Hanhao Shi, Hanhao Shi, Nazar Ali Korejo, Asghar Ali Kamboh, Rashid Ali Korejo, Fangxiong Shi

“…However, in the epididymis, germ cell diminution contraction of tubules, compacted principal and clear cells, lipid vacuolization, atypical cellular connections, exfoliated epithelial cells, and round spermatids were conspicuous in DH mice.DiscussionCollectively, our experiment was undertaken to ultimately better recognize male reproductive disorders in diabetic-hyperthyroid patients.…”
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Age-related decline in spermatogenic activity accompanied with endothelial cell senescence in male mice by Manabu Ozawa, Hideto Mori, Tsutomu Endo, Yu Ishikawa-Yamauchi, Daisuke Motooka, Chihiro Emori, Masahiro Ikawa

“…Interestingly, senescence-associated β-galactosidase-positive cells appeared in testicular endothelial cell (EC) populations, but not in germ cell populations, with aging. Transcriptome analysis of ECs indicated that senescence occurred in the ECs of aged mice. …”
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A genetic screen identifies new steps in oocyte maturation that enhance proteostasis in the immortal germ lineage by Madhuja Samaddar, Jérôme Goudeau, Melissa Sanchez, David H Hall, K Adam Bohnert, Maria Ingaramo, Cynthia Kenyon

“…Somatic cells age and die, but the germ-cell lineage is immortal. In Caenorhabditis elegans, germline immortality involves proteostasis renewal at the beginning of each new generation, when oocyte maturation signals from sperm trigger the clearance of carbonylated proteins and protein aggregates. …”
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A single-cell atlas reveals unanticipated cell type complexity in Drosophila ovaries by Slaidina, Maija, Gupta, Selena, Banisch, Torsten U, Lehmann, Ruth

“…We computed transcriptional signatures and identified specific markers for nine states of germ cell differentiation and 23 somatic cell types and subtypes. …”
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Different Roles for Tet1 and Tet2 Proteins in Reprogramming-Mediated Erasure of Imprints Induced by EGC Fusion by Piccolo, Francesco M., Bagci, Hakan, Brown, Karen E., Landeira, David, Soza-Ried, Jorge, Feytout, Amelie, Mooijman, Dylan, Hajkova, Petra, Leitch, Harry G., Tada, Takashi, Kriaucionis, Skirmantas, Dawlaty, Meelad M., Jaenisch, Rudolf, Merkenschlager, Matthias, Fisher, Amanda G.

“…Differential DNA methylation at imprinted control regions (ICRs) is established in gametes and, although largely preserved through development, can be experimentally reset by fusing somatic cells with embryonic germ cell (EGC) lines. Here, we show that the Ten-Eleven Translocation proteins Tet1 and Tet2 participate in the efficient erasure of imprints in this model system. …”
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Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions by Miller, Matthew P., Unal, Elcin, Brar, Gloria A., Amon, Angelika B

“…Determining how this unusual chromosome segregation behavior is established is central to understanding germ cell development. Here we show that preventing microtubule–kinetochore interactions during premeiotic S phase and prophase I is essential for establishing the meiosis I chromosome segregation pattern. …”
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Retinoic Acid Activates Two Pathways Required for Meiosis in Mice by Koubova, Jana, Hu, Yueh-Chiang, Bhattacharyya, Tanmoy, Soh, Ying Qi Shirleen, Gill, Mark E., Goodheart, Mary L., Hogarth, Cathryn A., Griswold, Michael D., Page, David C

“…Further, RA induction of Rec8, like that of Stra8, requires the germ-cell-intrinsic competence factor Dazl. Our findings strengthen the importance of RA and Dazl in the meiotic transition, provide important details about the Stra8 pathway, and open avenues to investigate early meiosis through analysis of Rec8 induction and function.…”
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Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue. by Tirabosco, R, Mangham, D, Rosenberg, A, Vujovic, S, Bousdras, K, Pizzolitto, S, De Maglio, G, Bakker, d, Di Francesco, L, Kalil, R, Athanasou, N, O'Donnell, P, McCarthy, E, Flanagan, A

“…We also confirm the expression of brachyury in hemangioblastomas, and for the first time demonstrates its expression in spermatogonia and testicular germ cell tumors by immunohistochemistry. Brachyury was not detected in a wide range of tumors including carcinomas, lymphomas, and sarcomas. …”

Whole-genome sequencing of spermatocytic tumors provides insights into the mutational processes operating in the male germline by Giannoulatou, E, Maher, G, Ding, Z, Gillis, A, Dorssers, L, Hoischen, A, Rajpert-De Meyts, E, WGS500 Consortium, McVean, G, Wilkie, A, Looijenga, L, Goriely, A

“…Adult male germline stem cells (spermatogonia) proliferate by mitosis and, after puberty, generate spermatocytes that undertake meiosis to produce haploid spermatozoa. Germ cells are under evolutionary constraint to curtail mutations and maintain genome integrity. …”

Identifying targets of protective antibodies against severe malaria in Papua, Indonesia using locally expressed domains of Plasmodium falciparum Erythrocyte Membrane Protein 1 by Rambhatla, JS, Tonkin-Hill, GQ, Takashima, E, Tsuboi, T, Noviyanti, R, Trianty, L, Sebayang, BF, Lampah, DA, Marfurt, J, Price, RN, Anstey, NM, Papenfuss, AT, Damelang, T, Chung, AW, Duffy, MF, Rogerson, SJ

“…We cloned and expressed domains from 32 PfEMP1s including 22 that were upregulated in severe malaria and 10 that were upregulated in uncomplicated malaria, using a wheat germ cell-free expression system. We used Luminex technology to measure IgG antibodies to these 32 domains and control proteins in 63 individuals (11 children). …”

Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies by Mohamed, E., Abdul-Rahman, P.S., Doustjaialil, S.R., Chen, Y., Lim, B.K., Omar, S.Z., Bustam, A.Z., Singh, V.A., Mohd-Taib, N., Yip, C.H., Hashim, Onn Haji

“…When compared to control sera (n = 17), expression of the 35 kDa ITIH4 cleavage fragment was demonstrated to be significantly enhanced in sera of patients with breast carcinoma (n = 10), epithelial ovarian carcinoma (n = 10), and germ cell ovarian carcinoma (n = 10) but not in patients with nasopharyngeal carcinoma (n = 13) and osteosarcoma (n = 7). …”
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SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing by Wenbo Liu, Xukun Lu, Zheng-Hui Zhao, Ruibao SU, Qian-Nan Li Li, Yue Xue, Zheng Gao, Si-Min Sun Sun, Wen-Long Lei, Lei Li, Geng An, Hanyan Liu, Zhiming Han, Ying-Chun Ouyang, Yi Hou, Zhen-Bo Wang, Qing-Yuan Sun, Jianqiao Liu

“…However, how alternative splicing regulates spermatogenesis remains largely unknown. Here, using a germ cell-specific knockout mouse model, we demonstrated that the splicing factor Srsf10 is essential for spermatogenesis and male fertility. …”
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Whole Exome Sequencing and In Silico Analysis of Human Sertoli in Patients with Non-Obstructive Azoospermia by Hossein Azizi, Danial Hashemi Karoii, Thomas Skutella

“…Our findings suggest that these genes and their interacting hub proteins could help determine the pathophysiologies of germ cell abnormalities and infertility.…”
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Lysophosphatidic Acid Signalling Regulates Human Sperm Viability via the Phosphoinositide 3-Kinase/AKT Pathway by Hao-Yu Liao, Cristian O’Flaherty

“…Lysophosphatidic acid (LPA) signalling is essential for maintaining germ cell viability during mouse spermatogenesis; however, its role in human spermatozoa is unknown. …”
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Large, three-generation human families reveal post-zygotic mosaicism and variability in germline mutation accumulation by Thomas A Sasani, Brent S Pedersen, Ziyue Gao, Lisa Baird, Molly Przeworski, Lynn B Jorde, Aaron R Quinlan

“…Additionally, we found that ~3% of DNMs originated following primordial germ cell specification in a parent, and differed from non-mosaic germline DNMs in their mutational spectra. …”
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Recent advances in developing 3D culture systems of spermatogonial stem cell preservation and differentiation: A narrative review by Leyla Fath-Bayati, Leila Naserpour, Mohadeseh Khoshandam, Rahil Jannatifar, Hoda Fazaeli

“…The production of functioning male germ cells is suggested by introducing modern bioengineered systems as a new hope for the maintenance of male fertility. …”
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