Y-chromosome genes associated with sertoli cell-only syndrome identified by array comparative genome hybridization by Kuo-Chung Lan, Hung-Jen Wang, Tzu-Jou Wang, Hsin-Jung Lin, Yung-Chiao Chang, Hong-Yo Kang

“…Results: Our initial aCGH experiments identified CDY1a and CDY1b double deletions in all 8 patients who had total germ cell depletion. However, 5 patients had DAZ1/2 and DAZ3/4 deletions, 1 patient had a DAZ2 and DAZ3/4 deletion, and 2 patients had no DAZ1/2 or DAZ3/4 deletions. …”
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Cell-Cell Interaction-Mediated Signaling in the Testis Induces Reproductive Dysfunction—Lesson from the Toxicant/Pharmaceutical Models by Lingling Wang, Tiao Bu, Xiaolong Wu, Sheng Gao, Xinyao Li, Angela Bryanne De Jesus, Chris K. C. Wong, Hao Chen, Nancy P. Y. Chung, Fei Sun, C. Yan Cheng

“…Emerging evidence has shown that cell-cell interactions between testicular cells, in particular at the Sertoli cell-cell and Sertoli-germ cell interface, are crucial to support spermatogenesis. …”
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D-Aspartate Depletion Perturbs Steroidogenesis and Spermatogenesis in Mice by Alessandra Santillo, Sara Falvo, Massimo Venditti, Anna Di Maio, Gabriella Chieffi Baccari, Francesco Errico, Alessandro Usiello, Sergio Minucci, Maria Maddalena Di Fiore

“…Our findings confirmed that physiological D-Asp influences testosterone biosynthesis and plays a crucial role in germ cell proliferation and differentiation, which are required for successful reproduction.…”
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Impact of Chronic Multi-Generational Exposure to an Environmentally Relevant Atrazine Concentration on Testicular Development and Function in Mice by Nicola D. Kolaitis, Bethany J. Finger, D. Jo Merriner, Joseph Nguyen, Brendan J. Houston, Moira K. O’Bryan, Jessica M. Stringer, Nadeen Zerafa, Ngoc Nguyen, Karla J. Hutt, Gerard A. Tarulli, Mark P. Green

“…Atrazine did not impact sperm function, testicular morphology nor germ cell parameters but did alter the expression of steroidogenic genes in the F1, down-regulating the expression of <i>Cyp17a1</i> (Cytochrome P450 family 17, subfamily A member 1; <i>p</i> = 0.0008) and <i>Ddx4</i> (DEAD-box helicase 4; <i>p</i> = 0.007), and up-regulating the expression of <i>Star</i> (Steroidogenic acute regulatory protein; <i>p</i> = 0.017). …”
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Cloning of the Maternal Effector Gene <i>org</i> and Its Regulation by lncRNA ORG-AS in Chinese Tongue Sole (<i>Cynoglossus semilaevis</i>) by Xiaona Zhao, Bo Feng, Qian Wang, Lili Tang, Qian Liu, Wenxiu Ma, Chenghua Li, Changwei Shao

“…The oogenesis-related gene <i>org</i> is a germ-cell-specific gene in fish, but the role of <i>org</i> in embryonic development and oogenesis has rarely been studied, and the knowledge of the lncRNA-mediated regulation of <i>org</i> is limited. …”
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Testicular Expression of Antioxidant Enzymes and Changes in Response to a Slow-Release Deslorelin Implant (Suprelorin^® 4.7 mg) in the Dog by Duygu Yaman Gram, Brigid Sexton, Narin Liman, Linda Müller, Murat Abay, Aykut Gram, Orsolya Balogh

“…Different expression patterns of antioxidant enzymes in various germ cell populations and stages of the seminiferous epithelium cycle may indicate differences in their susceptibility to oxidative stress depending on their developmental and maturation stage. …”
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The ovarian germinal reserve and apoptosis-related proteins in the infant and adolescent human ovary by María Itatí Albamonte, Mirta Susana Albamonte, Ricardo M. Bou-Khair, Luis Zuccardi, Alfredo Daniel Vitullo

“…We evaluated the expression of pro- and anti-apoptotic members of the BCL2 gene family, the FAS/FAS-L proteins from the extrinsic apoptosis pathway, the germ-cell-specific marker VASA, the pluripotency marker OCT3/4, and markers of early and late apoptosis in the ovary of pubertal patients with malignant extra-gonadal disease, which received or not pre-surgery chemotherapy, entering a cryopreservation program. …”
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RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3 by Manami SENOO, Hiroshi HOZOJI, Yu ISHIKAWA-YAMAUCHI, Takashi TAKIJIRI, Sho OHTA, Tomoyo UKAI, Mio KABATA, Takuya YAMAMOTO, Yasuhiro YAMADA, Masahito IKAWA, Manabu OZAWA

“…Furthermore, Nanos3+/−;Ptbp1+/− mice exhibited abnormal spermatogenesis, which resembled the effects of germ cell-specific Ptbp1 KO, whereas no significant abnormality was observed in mice heterozygous for either gene alone. …”
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Single-cell RNA-sequencing reveals the transcriptional landscape of ND-42 mediated spermatid elongation via mitochondrial derivative maintenance in Drosophila testes by Jun Yu, Zhiran Li, Yangbo Fu, Feiteng Sun, Xia Chen, Qiuru Huang, Lei He, Hao Yu, Li Ji, Xinmeng Cheng, Yi Shi, Cong Shen, Bo Zheng, Fei Sun

“…Based on single-cell RNA-sequencing (scRNA-seq), we identified 15 distinct cell clusters, including several unanticipated transitional subpopulations or differentiative stages for testicular germ cell complexity in Drosophila testes. Enrichments of the transcriptional regulatory network in the late-stage cell populations revealed key roles of ND-42 in mitochondria and its related biological processes during spermatid elongation. …”
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Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian Propolis and Metformin... by Victor Udo Nna, Ainul Bahiyah Abu Bakar, Azlina Ahmad, Chinedum Ogbonnaya Eleazu, Mahaneem Mohamed

“…MP also down-regulated (<i>p</i> &lt; 0.05) Bax/Bcl-2, p53, casapase-8, caspase-9 and caspase-3 genes, and increased (<i>p</i> &lt; 0.05) testicular germ cell proliferation. MP&#8217;s effects were comparable to Met. …”
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CT Rendering and Radiomic Analysis in Post-Chemotherapy Retroperitoneal Lymph Node Dissection for Testicular Cancer to Anticipate Difficulties for Young Surgeons by Anna Scavuzzo, Pavel Figueroa-Rodriguez, Alessandro Stefano, Nallely Jimenez Guedulain, Sebastian Muruato Araiza, Jose de Jesus Cendejas Gomez, Alejandro Quiroz Compeaán, Dimas O. Victorio Vargas, Miguel A. Jiménez-Ríos

“…Post-chemotherapy retroperitoneal lymph node dissection (PC-RPLND) in non-seminomatous germ-cell tumor (NSTGCTs) is a complex procedure. We evaluated whether 3D computed tomography (CT) rendering and their radiomic analysis help predict resectability by junior surgeons. …”
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Evaluation of the Perioperative and Postoperative Course of Surgery for Pineal Germinoma in the SIOP CNS GCT 96 Trial by Ehab Shabo, Thomas Czech, James C. Nicholson, Conor Mallucci, Carmine Mottolese, Gianluca Piatelli, Didier Frappaz, Matthew Jonathan Murray, Cecile Faure-Conter, Maria Luisa Garrè, Sevgi Sarikaya-Seiwert, Leonie Weinhold, Hannes Haberl, Gabriele Calaminus

“…In contrast to the suprasellar region, where biopsy is the standard procedure in case of a suspected germ-cell tumor to avoid mutilation to the endocrine structures, pineal tumors are more accessible to primary resection. …”
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The effects of endogenous FSH and its receptor on oogenesis and folliculogenesis in female Alligator sinensis by Haitao Nie, Yunlu Xu, Yuqian Zhang, Yue Wen, Jixiang Zhan, Yong Xia, Yongkang Zhou, Renping Wang, Xiaobing Wu

“…The presence of endogenous follicle-stimulating hormone(FSH) mRNA and protein expression within the cerebrum at 1 dph, in parallel with the increase in germ cells within the germ cell nests(Nest) from 1 dph to 15 dph, suggested that endocrine regulation of the pituitary-gonad axis is an early event in oogonia division. …”
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Stem Cell Transplantation Therapy and Neurological Disorders: Current Status and Future Perspectives by Md. Mominur Rahman, Md. Rezaul Islam, Mohammad Touhidul Islam, Md. Harun-Or-Rashid, Mahfuzul Islam, Sabirin Abdullah, Mohammad Borhan Uddin, Sumit Das, Md. Saidur Rahaman, Muniruddin Ahmed, Fahad A. Alhumaydhi, Talha Bin Emran, Amany Abdel-Rahman Mohamed, Mohammad Rashed Iqbal Faruque, Mayeen Uddin Khandaker, Gomaa Mostafa-Hedeab

“…When a stem cell divides, each new cell has the potential to either remain a stem cell or differentiate into a germ cell with specialized characteristics, such as muscle cells, red blood cells, or brain cells. …”
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What do we know about blood-testis barrier? current understanding of its structure and physiology by J. P. Luaces, N. Toro-Urrego, M. Otero-Losada, F. Capani, F. Capani

“…Thus, these specialized structures facilitate germ cell movement across the BTB. Junctions are constantly rearranged during spermatogenesis while the BTB preserves its barrier function. …”
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Identification and characterization of dystrophin-locus-derived testis-specific protein: A testis-specific gene within the intronic region of the rat dystrophin gene by Keitaro YAMANOUCHI, Shizuka KATO, Yukie TANAKA, Masanari IKEDA, Yukina OSHIMO, Takanori SHIGA, Kei HATAMOTO, James CHAMBERS, Takuya IMAMURA, Ryuji HIRAMATSU, Kazuyuki UCHIDA, Fuko MATSUDA, Takashi MATSUWAKI, Tetsuya KOHSAKA

“…The mammalian X chromosome exhibits enrichment in genes associated with germ cell development. Previously, we generated a rat model of Becker muscular dystrophy (BMD) characterized by an in-frame mutation in the dystrophin gene, situated on the X chromosome and responsible for encoding a protein crucial for muscle integrity. …”
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Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells by Meng Zhou, Manling Zhang, Tianxu Guo, Lihua Zhao, Lihua Zhao, Xiyun Guo, Zhibao Yin, Linxin Cheng, Han Liu, Lixia Zhao, Xihe Li, Rongfeng Li, Rongfeng Li

“…Porcine TFs-derived piPSCs (ppiPSCs) showed the highest levels of endogenous pluripotency markers activation, were able to differentiate into three germ layers and primordial germ cell-like cells (PGCLCs) and integrated into neural ectoderm of E7.5 mouse embryos in vitro. …”
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A Gene Regulatory Program for Meiotic Prophase in the Fetal Ovary by Junker, Jan Philipp, Gill, Mark E., Mueller, Jacob L., van Oudenaarden, Alexander, Soh, Ying Qi Shirleen, Page, David C

“…By profiling gene expression in the mouse fetal ovary in mutants with whole tissue and single-cell techniques, we identified 104 genes expressed specifically in pre-meiotic to pachytene germ cells. We characterized the regulation of these genes by 1) retinoic acid (RA), which induces meiosis, 2) Dazl, which is required for germ cell competence to respond to RA, and 3) Stra8, a downstream target of RA required for the chromosomal program of meiotic prophase. …”
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Fertility preservation in pre-pubertal boys with cancer: a three-dimensional (3D) prepubertal testicular organoid culture system for in vitro spermatogonial stem cell (SSCs) propag... by Tang, S

“…Firstly, I optimized the dissociation and enrichment of germ cells in neonatal bovine ITTs. Next, by comparing different extracellular matrix (ECM), I found that Matrigel was optimal in the formation of germ cell aggregations. …”

Incidentally detected testicular lesions <10 mm in diameter: can orchidectomy be avoided? by Scandura, G, Verrill, C, Protheroe, A, Joseph, J, Ansell, W, Sahdev, A, Shamash, J, Berney, D

“…In total there were 25/81 malignant cases (31%), which were all germ cell tumours (GCTs): 15 seminomas (60%) and 10 non‐seminomatous GCTs (40%). …”