USE OF INTERMEDIATE-DOSE CYTARABINE PLUS G-CSF AS PERIPHERAL BLOOD STEM CELL MOBILIZATION PROTOCOL IN 311 PATIENTS WITH ONCO-HEMATOLOGICAL DISEASES: RESULTS FROM A SINGLE INSTITUTI... by AF Callera, ES Rosa, F Callera

“…From January 2016 to July 2023, 311 patients (204 MM, 64 non-Hodgkin's lymphoma, 35 Hodgkin's lymphoma, 5 germ cell tumor and 3 acute non-promyelocytic leucemia, median age of 56 years, range 17‒74) were mobilized with Ara-C plus G-CSF. …”
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Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells by Shuaiqi Han, Hongyun Zhang, Xinyu Liu, Fei Wen, Baiyu Li, Zhiwen Bie, Hehan Qiu, Jianhong Hu

“…Collectively, our study suggests that AFB1 inhibits the secretion of lactate which supply for germ cell development by damaging the ''Warburg-like'' metabolism of dairy goat SCs. …”
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Chat GPT in Diagnostic Human Pathology: Will It Be Useful to Pathologists? A Preliminary Review with ‘Query Session’ and Future Perspectives by Gerardo Cazzato, Marialessandra Capuzzolo, Paola Parente, Francesca Arezzo, Vera Loizzi, Enrica Macorano, Andrea Marzullo, Gennaro Cormio, Giuseppe Ingravallo

“…Furthermore, we performed a ‘query session’ with ChatGPT regarding pathologies such as pigmented skin lesions, malignant melanoma and variants, Gleason’s score of prostate adenocarcinoma, differential diagnosis between germ cell tumors and high grade serous carcinoma of the ovary, pleural mesothelioma and pediatric diffuse midline glioma. …”
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Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis by Enrico Radaelli, Charles-Antoine Assenmacher, Jillian Verrelle, Esha Banerjee, Florence Manero, Salim Khiati, Anais Girona, Guillermo Lopez-Lluch, Placido Navas, Marco Spinazzi

“…These mitochondrial defects are associated with a germ cell-specific decrease in GPX4 expression leading arrested spermatocytes to ferroptosis – a regulated cell death modality characterized by uncontrolled lipid peroxidation. …”
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Inceptor facilitates acrosomal vesicle formation in spermatids and is required for male fertility by Sara Bilekova, Sara Bilekova, Sara Bilekova, Balma Garcia-Colomer, Balma Garcia-Colomer, Alberto Cebrian-Serrano, Alberto Cebrian-Serrano, Silvia Schirge, Silvia Schirge, Karsten Krey, Karsten Krey, Michael Sterr, Michael Sterr, Thomas Kurth, Stefanie M. Hauck, Stefanie M. Hauck, Heiko Lickert, Heiko Lickert, Heiko Lickert

“…Spermatogenesis is a crucial biological process that enables the production of functional sperm, allowing for successful reproduction. Proper germ cell differentiation and maturation require tight regulation of hormonal signals, cellular signaling pathways, and cell biological processes. …”
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Germline ablation achieved via CRISPR/Cas9 targeting of NANOS3 in bovine zygotes by Maci L. Mueller, Bret R. McNabb, Joseph R. Owen, Sadie L. Hennig, Alba V. Ledesma, Mitchell L. Angove, Alan J. Conley, Pablo J. Ross, Alison L. Van Eenennaam

“…NANOS3 is expressed in migrating primordial germ cells (PGCs) to protect them from apoptosis, and it is known to be a critical factor for germline development of both sexes in several organisms. …”
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Yolk sac tumor and dysgerminoma in the left gonad following gonadoblastoma in the right gonad in a 46,XY DSD with a novel SRY missense mutation: a case report by Chengxiu Xie, Jian Cai, Nan Li, Ping Hua, Zexuan Yang, Xia Yu, Dongmei Tang, Yu Hu, Qingsong Liu

“…Here, we present a case of 46,XY DSD caused by a novel missense mutation in the HMG region of SRY rapidly progressing to germ cell tumors (GCTs). Case presentation An adolescent female (15 years old) exhibiting primary amenorrhea was later diagnosed as a 46,XY female with bilateral gonadal dysplasia on the basis of peripheral lymphocyte karyotype 46,XY and a novel missense mutation in SRY (c.281 T > G, p.L94R). …”
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Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells by Diba Ghasemi, Somayeh Ebrahimi-Barough, Mohammad Hossein Nekoofar, Abdolreza Mohamadnia, Nasrin Lotfibakhshaiesh, Naghmeh Bahrami, Roya Karimi, Vajihe Taghdiri Nooshabadi, Mahmoud Azami, Elham Hasanzadeh, Jafar Ai

“…Methods: We adjusted the optimum retinoic acid (RA) concentration for enhancement of germ cell-derived hEnSCs generation in 2D cell culture after 7 days in this study. …”
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Pan-Cancer Analysis Reveals SH3TC2 as an Oncogene for Colorectal Cancer and Promotes Tumorigenesis via the MAPK Pathway by Chengzhi Huang, Hui Yi, Yue Zhou, Qing Zhang, Xueqing Yao

“…We observed differential expression of <i>SH3TC2</i> in colon adenocarcinoma (COAD), acute myeloid leukemia (LAML), READ (rectum adenocarcinoma), SKCM (skin cutaneous melanoma), and TGCT (testicular germ cell tumors). Subsequently, <i>SH3TC2</i> showed a significant effect on the clinical stage and prognostic value in CRC, LAML, and SKCM. …”
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Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids by Marika Berardini, Luisa Gesualdi, Caterina Morabito, Francesca Ferranti, Anna Reale, Michele Zampieri, Katsiaryna Karpach, Antonella Tinari, Lucia Bertuccini, Simone Guarnieri, Angela Catizone, Maria A. Mariggiò, Giulia Ricci

“…It is well known that male germ cells are sensitive to oxidative stress and to changes in gravitational force, even though published data on germ cell models are scarce. …”
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Potential Gonado-Protective Effect of <i>Cichorium endivia</i> and Its Major Phenolic Acids against Methotrexate-Induced Testicular Injury in Mice by Enas E. Eltamany, Esraa M. Mosalam, Eman T. Mehanna, Basma M. Awad, Sarah M. Mosaad, Maged S. Abdel-Kader, Amany K. Ibrahim, Jihan M. Badr, Marwa S. Goda

“…In conclusion, <i>C. endivia</i> mitigated MTX-induced germ cell toxicity via anti-inflammatory, antioxidant, and antiapoptotic effects.…”
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Transcriptome profiling of the gonad during sex differentiation in hermaphroditic brown-marbled grouper (Epinephelus fuscoguttatus) by Xi Wu, Yang Yang, Tong Wang, Chaoyue Zhong, Yuhao Tao, Leyi Chang, Zining Meng, Xiaochun Liu

“…The germ cells in 259 dph gonad were classified into six stages by their histological morphology. …”
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Ultrasonic misdiagnosis of giant pediatric testicular yolk sac tumor: A case report and literature review by Zilong Wang, Fuding Lu, Changze Song, Xinkun Wang, Naifa Li, Jiawen Zhai, Baohong Jiang, Jianpeng Yuan, Zheng Yang, Xujun Xuan, Xujun Xuan

“…BackgroundYolk sac tumor is the most common malignant nonseminomatous germ-cell tumor in children characterized by elevated level of α-fetoprotein (AFP), accounting for 70%–80% of all cases. …”
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Develop and validate nomogram to predict cancer-specific survival for patients with testicular yolk sac tumors by Maoxian Li, Maoxian Li, Jinkui Wang, Jinfeng Li, Yongbo Zhang, Xing Zhao, Yang Lin, Changkai Deng, Fulin Li, Qiang Peng

“…PurposeTesticular yolk sac tumor (TYST) is a rare malignant germ cell tumor that mainly occurs in young men. Due to the low incidence of yolk sac tumors, there is a lack of prospective cohort studies with large samples. …”
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Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice by Qianyu Zhang, Qianyu Zhang, Zhifu Wei, Huinan Weng, Ye Chen, Jie Zhang, Shiwei Mei, Jiahui Wei, Xiulan Zhu, Yingqi Nong, Jianxing Ruan, Wenjuan Liu, Ruiqiong Zhou, Fang Wang, Yanni Xie, Junjiu Huang, Xiqian Zhang, Fenghua Liu

“…Our results indicated that FA intervention effectively alleviated ovarian damage, leading to more regular estrous cycles, lesser impairment of follicular morphology and endocrine status, as well as greater germ cell preservation. Reduced levels of oxidative stress, inflammation, and enhanced DNA repair were associated these changes. …”
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Childhood-onset systemic lupus erythematosus (cSLE) and malignancy: a nationwide multicentre series review by Matheus Zanata Brufatto, Sean Hideo Shirata Lanças, Taciana de Albuquerque Pedrosa Fernandes, Adriana Maluf Elias Sallum, Lucia Maria Arruda Campos, Ana Paula Sakamoto, Maria Teresa Terreri, Flavio Roberto Sztajnbok, Blanca Elena Rios Gomes Bica, Virginia Paes Leme Ferriani, Luciana Martins de Carvalho, Clovis Artur Almeida Silva, Claudia Saad-Magalhaes

“…Median (min–max) SLEDAI-2 K scores were 9 (0–38), median (min–max) SLICC/ACR-DI (SDI) score were 1 (1–5) Histopathology defined 1 Hodgkin's lymphoma, 2 non-Hodgkin's lymphoma, 1 acute lymphoblastic leukaemia; 4 gastrointestinal carcinoma, 1 squamous cell carcinoma of the tongue and 1 anal carcinoma; 1 had sigmoid adenocarcinoma and 1 stomach carcinoid; 3 had genital malignancy, being 1 vulvae, 1 cervix and 1 vulvae and cervix carcinomas; 1 had central nervous system oligodendroglioma; and 1 testicle germ cell teratoma. Conclusion Estimated malignancy frequency of 0.7% was reported during cSLE follow up in a multicentric series. …”
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Onco-TESE (Testicular Sperm Extraction): Insights from a Tertiary Center and Comprehensive Literature Analysis by Lorenzo Cirigliano, Marco Falcone, Murat Gül, Mirko Preto, Carlo Ceruti, Natalia Plamadeala, Federica Peretti, Ilaria Ferro, Martina Scavone, Paolo Gontero

“…<i>Results</i>: A total of nine patients were included, with a median age of 34 (IQR 29–36) years. All patients had germ cell tumors (GCTs), with seminomatous and non-seminomatous GCTs accounting for 44.4% (<i>n</i> = 4) and 55.6% (<i>n</i> = 5) of patients, respectively. …”
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Emerging roles and potential application of PIWI-interacting RNA in urological tumors by Jingcheng Zhang, Jingcheng Zhang, Wentao Zhang, Wentao Zhang, Yuchao Liu, Yuchao Liu, Man Pi, Yufeng Jiang, Yufeng Jiang, Ailiyaer Ainiwaer, Ailiyaer Ainiwaer, Shiyu Mao, Shiyu Mao, Haotian Chen, Haotian Chen, Yuefei Ran, Yuefei Ran, Shuwen Sun, Shuwen Sun, Wei Li, Wei Li, Xudong Yao, Xudong Yao, Zhengyan Chang, Yang Yan, Yang Yan

“…It was originally found in germ cells and is considered a regulator of germ cell function. …”
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Effects of 17α-Methyltestosterone on the Transcriptome and Sex Hormones in the Brain of <i>Gobiocypris rarus</i> by Shaozhen Liu, Yue Chen, Tongyao Li, Liying Qiao, Qiong Yang, Weiya Rong, Qing Liu, Weiwei Wang, Jing Song, Xianzong Wang, Yu Liu

“…17α-Methyltestosterone (MT), a synthetic environmental endocrine disruptor with androgenic effects, has been shown to disrupt the reproductive system and inhibit germ cell maturation in <i>Gobiocypris rarus</i>. To further investigate the regulation of gonadal development by MT through the hypothalamic-pituitary-gonadal (HPG) axis, <i>G. rarus</i> were exposed to 0, 25, 50, and 100 ng/L of MT for 7, 14, and 21 days. …”
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Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for peritoneal malignant tumors in children: Initial experience in a single institution by Zhiyun Zhu, Xiaofeng Chang, Jiarong Wang, Shen Yang, Hong Qin, Wei Yang, Haiyan Cheng, Deguang Meng, Huanmin Wang

“…The histologic types included rhabdomyosarcoma (7), Wilms tumor (2), clear cell sarcoma of the kidney (2), undifferentiated sarcoma (2), immature teratoma (1), peritoneal serous carcinoma (1), malignant rhabdoid of the kidney (1), malignant germ cell tumor (1), neuroblastoma (1), and epithelioid inflammatory myofibroblast sarcoma (1). …”
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