Dissecting Human Gonadal Cell Lineage Specification and Sex Determination Using A Single-cell RNA-seq Approach by Rui Wang, Xixi Liu, Li Li, Ming Yang, Jun Yong, Fan Zhai, Lu Wen, Liying Yan, Jie Qiao, Fuchou Tang

“…Gonadal somatic cells are the main players in gonad development and are important for sex determination and germ cell development. Here, using a time-series single-cell RNA sequencing (scRNA-seq) strategy, we analyzed fetal germ cells (FGCs) and gonadal somatic cells in human embryos and fetuses. …”
Get full text

Fertility restoration in mice with chemotherapy induced ovarian failure using differentiated iPSCsResearch in context by Kevin M. Elias, Nicholas W. Ng, Kh U. Dam, Ankrish Milne, Emily R. Disler, Alison Gockley, Nicole Holub, Maya L. Seshan, George M. Church, Elizabeth S. Ginsburg, Raymond M. Anchan

“…Findings: [1] iPSCs differentiate into functional oocytes and steroidogenic ovarian cells which [2] express an ovarian (GJA1) and germ cell (ZP1) markers. [3] Endocrine function and fertility were restored in mice pretreated with gonadotoxic alkylating agents via orthotopic transplantation of differentiated iPSCS, thus generating viable, fertile mouse pups. …”
Get full text

9th International Congress of Medical Oncology & Hematology of Iran; 26-28 Nov., 2009 Olympic Hotel Tehran-Iran by A A

“…Shiraz University of Medical Sciences, Iran 12 ◘ High Dose Chemotherapy and Autologous Stem Cell Transplantation in Germ Cell Tumor Mehdizadeh M., Tabaraie M., Haj Fathali A., Atariyan H., Esfehani F., Rezvani H., Ghadyani M., Chegeni R. …”
Get full text

Genomic study of TEX15 variants: prevalence and allelic heterogeneity in men with spermatogenic failure by Sidra Qureshi, Jimmaline J. Hardy, Christopher Pombar, Andrea J. Berman, Agnieszka Malcher, Tara Gingrich, Rachel Hvasta, Jannah Kuong, Sarah Munyoki, Kathleen Hwang, Kyle E. Orwig, Jawad Ahmed, Marta Olszewska, Maciej Kurpisz, Donald F. Conrad, Muhammad Jaseem Khan, Alexander N. Yatsenko, Alexander N. Yatsenko, Alexander N. Yatsenko

“…In particular, the function of many meiotic proteins encoded by germ cell specific genes is critical for maturation of haploid spermatids and viable spermatozoa, necessary for fertilization, and is also extremely sensitive to even the slightest change in coding DNA.Methods: Here, using whole exome and genome approaches, we identified and reported novel, clinically significant variants in testis-expressed gene 15 (TEX15), in unrelated men with spermatogenic failure (SPGF).Results: TEX15 mediates double strand break repair during meiosis. …”
Get full text

COVID-19 Infection Induce miR-371a-3p Upregulation Resulting in Influence on Male Fertility by Heike Goebel, Barbara Koeditz, Manuel Huerta, Ersen Kameri, Tim Nestler, Thomas Kamphausen, Johannes Friemann, Matthias Hamdorf, Timo Ohrmann, Philipp Koehler, Oliver A. Cornely, Manuel Montesinos-Rongen, David Nicol, Hubert Schorle, Peter Boor, Alexander Quaas, Christian Pallasch, Axel Heidenreich, Melanie von Brandenstein

“…To study the influence of SARS-CoV-2 which cause a dysregulation of the androgen receptor (AR) level, thereby leading to fertility problems and inducing germ cell testicular changes in patients after the infection. …”
Get full text

Gastric undifferentiated carcinoma, INI1-negative by Gabriele Gaggero, Margherita Concardi, Marta Ingaliso, Davide Taietti

“…From a strictly microscopic point of view, the features of the neoplastic cells suggest the differential diagnosis between a GUC, an EBV-associated carcinoma with lymphoid stroma, an aggressive lymphoma, a metastatic melanoma, a germ cell neoplasm, a PEComa, and a rhabdomyosarcoma (or other types of sarcomas with epithelioid/rhabdoid pattern).1 Based on the overall immunomorphological data, which allows us to exclude the other aforementioned hypotheses, we make the final diagnosis of GUC with rhabdoid morphological aspects and, according to the literature, lack of immunophenotypic expression for INI1. …”
Get full text

Baseline characteristics and patterns of care in testicular cancer patients: first data from the Swiss Austrian German Testicular Cancer Cohort Study (SAG TCCS) by Christian Rothermundt, Claudio Thurneysen, Richard Cathomas, Beat Müller, Walter Mingrone, Anita Hirschi-Blickenstorfer, Tobias Wehrhahn, Christian Ruf, Sacha I. Rothschild, Bettina Seifert, Angelika Terbuch, Thomas Grassmugg, Regina Woelky, Christian Fankhauser, Thomas Kunit, Natalie Fischer, Roman Inauen, Katrin Ziegler, Alan Haynes, Peter Jüni, Jeanine Kehl, Silke Gillessen

“… BACKGROUND The majority of germ cell tumour (GCT) patients can be cured by orchiectomy followed by active surveillance or subsequent systemic and/or local treatments. …”
Get full text

Late effects of cancer in children, teenagers and young adults: Population-based study on the burden of 183 conditions, in-patient and critical care admissions and years of life lo... by Wai Hoong Chang, Michail Katsoulis, Yen Yi Tan, Stefanie H. Mueller, Katherine Green, Alvina G. Lai

“…The cumulative burden of late effects at age 35 was the highest in survivors of leukaemia (23.52 per individual [95% CI:19.85–29.33]) and lowest in survivors of germ cell tumours (CI:6.04 [5.32–6.91]). In controls, the cumulative burden was 3.99 (CI:3.93–4.08) at age 35 years. …”
Get full text

Dynamic regulation of P-TEFb by 7SK snRNP is integral to the DNA damage response to regulate chemotherapy sensitivity by Yin Fang, Yan Wang, Benjamin M. Spector, Xue Xiao, Chao Yang, Ping Li, Yuan Yuan, Ping Ding, Zhi-Xiong Xiao, Peixuan Zhang, Tong Qiu, Xiaofeng Zhu, David H. Price, Qintong Li

“…Summary: Testicular germ cell tumors and closely related embryonal stem cells are exquisitely sensitive to cisplatin, a feature thought to be linked to their pluripotent state and p53 status. …”
Get full text

Expression and Characterization of the <i>Spats1</i> Gene and Its Response to E2/MT Treatment in the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>) by Luo Lei, Junxian Zhu, Chen Chen, Yakun Wang, Xiaoyou Hong, Xiaoli Liu, Lingyun Yu, Chengqing Wei, Haigang Chen, Yihui Liu, Ruiyang Li, Wei Li, Xinping Zhu

“…<i>Spats1</i> (spermatogenesis-associated, serinerich 1) has been characterized as a male-biased gene which acts an important role in the germ cell differentiation of mammals. Nevertheless, the function of <i>Spats1</i> in the Chinese soft-shelled turtle (<i>P. sinensis</i>) has not yet been reported. …”
Get full text

Reducedhumoral response against variants of concern in childhood solid cancer patients compared to adult patients and healthy children after SARS-CoV-2 vaccination by Yifei Ma, Yifei Ma, Bocen Chen, Yanqi Wang, Yanqi Wang, Pengfei Zhu, Nianqi Liu, Zhiying Zhang, Zhiying Zhang, Guanqing Zhong, Guangzhen Fu, Dao Wang, Lu Cao, Shenrui Bai, Youlong Wang, Shuqin Chen, Xiaolong Wei, Jun Lv, Ao Zhang, Xinjia Wang, Xinjia Wang

“…Pathology included carcinoma, neural tumors, sarcoma, and germ cell tumors. Median chemotherapy time was 7 (quartile, 5–11) months. …”
Get full text

High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients by Guilherme M. J. Costa, Samyra M. S. N. Lacerda, André F. A. Figueiredo, Natália T. Wnuk, Marcos R. G. Brener, Lídia M. Andrade, Gabriel H. Campolina-Silva, Andrea Kauffmann-Zeh, Lucila G. G. Pacifico, Alice F. Versiani, Maísa M. Antunes, Fernanda R. Souza, Geovanni D. Cassali, André L. Caldeira-Brant, Hélio Chiarini-Garcia, Fernanda G. de Souza, Vivian V. Costa, Flavio G. da Fonseca, Maurício L. Nogueira, Guilherme R. F. Campos, Lucas M. Kangussu, Estefânia M. N. Martins, Loudiana M. Antonio, Cintia Bittar, Paula Rahal, Renato S. Aguiar, Bárbara P. Mendes, Marcela S. Procópio, Thiago P. Furtado, Yuri L. Guimaraes, Gustavo B. Menezes, Ana Martinez-Marchal, Kyle E. Orwig, Miguel Brieño-Enríquez, Marcelo H. Furtado

“…The infected testes show thickening of the tunica propria, germ cell apoptosis, Sertoli cell barrier loss, evident hemorrhage, angiogenesis, Leydig cell inhibition, inflammation, and fibrosis. …”
Get full text

Understanding the functions of HMGB proteins in the mechanism of action of cisplatin by Park, Semi, Ph. D. Massachusetts Institute of Technology

Get full text

Resetting histone modifications during human prenatal germline development by Rui Gao, Shiyang Zeng, Dongxu Yang, Xiaocui Li, Wenqiang Liu, Yawei Gao, Dandan Bai, Linfeng Zhang, Chuan Chen, Yunzhe Kang, Beiying Wang, Wei Hong, Mingzhu Wang, Jiqing Yin, Hong Wang, Qiaolin Deng, Shaorong Gao, Yong Zhang, Jiayu Chen

“…Here, we map the genome-wide profiles of three key histone modifications in human primordial germ cells (hPGCs) from weeks 8 to 23 of gestation for the first time by performing ULI-NChIP-seq. …”
Get full text

The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis by Ziqi Wang, Tongtong Li, Dongkai Liu, Mengjing Li, Shangming Liu, Xiaochen Yu, Hanzhen Li, Hui Song, Wei Zhao, Zhaojian Liu, Xiangfeng Chen, Gang Lu, Zi-Jiang Chen, Tao Huang, Hongbin Liu

“…Summary: Spermiogenesis defines the final phase of male germ cell differentiation. While multiple deubiquitinating enzymes have been linked to spermiogenesis, the impacts of deubiquitination on spermiogenesis remain poorly characterized. …”
Get full text

Identification of quiescent FOXC2+ spermatogonial stem cells in adult mammals by Zhipeng Wang, Cheng Jin, Pengyu Li, Yiran Li, Jielin Tang, Zhixin Yu, Tao Jiao, Jinhuan Ou, Han Wang, Dingfeng Zou, Mengzhen Li, Xinyu Mang, Jun Liu, Yan Lu, Kai Li, Ning Zhang, Jia Yu, Shiying Miao, Linfang Wang, Wei Song

“…During germline regeneration, FOXC2+ SSCs were activated and able to completely restore the process. Germ cell-specific Foxc2 knockout resulted in an accelerated exhaustion of SSCs and eventually led to male infertility. …”
Get full text

Characteristics and outcomes of pediatric testicular yolk Sac tumor by Maoxian Li, Maoxian Li, Jinkui Wang, Jinkui Wang, Jianyu Wang, Jianyu Wang, Deying Zhang, Deying Zhang, Yi Hua, Yi Hua, Feng Liu, Feng Liu, Peng Lu, Peng Lu, Junhong Liu, Junhong Liu, Xing Liu, Xing Liu, Tao Lin, Tao Lin, Guanghui Wei, Guanghui Wei, Dawei He, Dawei He, Dawei He

“…PurposePediatric testicular yolk sac tumor is a rare malignant germ cell tumor and there is a lack of large clinical studies. …”
Get full text

A homozygous missense variant in DND1 causes non-obstructive azoospermia in humans by Xuefeng Xie, Mazhar Khan, Muhammad Zubair, Abbas Khan, Ranjha Khan, Jianteng Zhou, Yuanwei Zhang, Muzafar Said, Sher Ali Khan, Qamar Zaman, Ghulam Murtaza, Muzamil Ahmad Khan, Wei Liu, Xiaoning Hou, Huan Zhang, Bo Xu, Xiaohua Jiang, Shun Bai, Qinghua Shi

“…This mutation is predicted to cause DND1 protein misfolding and weaken the DND1 interaction with NANOS2, a significant regulator in primordial germ cell development. Our study identified a DND1 pathogenic mutation in NOA patients and highlighted its critical role in male fertility in humans.…”
Get full text

New Entity—Thalassemic Endocrine Disease: Major Beta-Thalassemia and Endocrine Involvement by Mara Carsote, Cristina Vasiliu, Alexandra Ioana Trandafir, Simona Elena Albu, Mihai-Cristian Dumitrascu, Adelina Popa, Claudia Mehedintu, Razvan-Cosmin Petca, Aida Petca, Florica Sandru

“…Males with BTH are at risk of infertility due to germ cell loss. IO induces an excessive amount of free radicals which impair the quality of sperm, iron being a local catalyser of ROS. …”
Get full text

Impact of the hypoxic microenvironment on spermatogonial stem cells in culture by A. S. Gille, A. S. Gille, A. S. Gille, A. S. Gille, M. Givelet, M. Givelet, M. Givelet, M. Givelet, D. Pehlic, D. Pehlic, C. Lapoujade, C. Lapoujade, B. Lassalle, B. Lassalle, V. Barroca, V. Barroca, A. P. Bemelmans, D. Borderie, D. Borderie, D. Moison, D. Moison, G. Livera, G. Livera, L. R. Gauthier, L. R. Gauthier, F. D. Boussin, F. D. Boussin, N. Thiounn, I. Allemand, I. Allemand, C. Peyssonnaux, C. Peyssonnaux, J. P. Wolf, J. P. Wolf, V. Barraud-Lange, V. Barraud-Lange, L. Riou, L. Riou, P. Fouchet, P. Fouchet

“…We observed a deleterious effect of severe hypoxia (1% O2 and 0.1% O2) on the capacity of murine SSCs to form germ cell clusters when plated at low density. Severe effects on SSCs proliferation occur at an O2 tension ≤1% and hypoxia was shown to induce a slight differentiation bias under 1% and 0.1% O2 conditions. …”
Get full text