Technical evaluation and usability of a quantitative G6PD POC test in cord blood: a mixed-methods study in a low-resource setting by Bancone, G, Gilder, ME, Win, E, Gornsawun, G, Penpitchaporn, P, Moo, PK, Archasuksan, L, Wai, NS, Win, S, Aung, KK, Hashmi, A, Hanboonkunupakarn, B, Nosten, F, Carrara, VI, McGready, R

“…<br><br> <strong>Results</strong> In 307 cord blood samples, the Biosensor had a sensitivity of 1.000 (95% CI: 0.859 to 1.000) and a specificity of 0.993 (95% CI: 0.971 to 0.999) as compared with gold-standard spectrophotometry to diagnose G6PD-deficient newborns using a receiver operating characteristic (ROC) analysis-derived threshold of ≤4.8 IU/gHb. …”

Evaluation of antenatal rapid human immunodeficiency virus testing in rural South Africa by Mashamba-Thompson, TP, Moodley, P, Sartorius, B, Drain, PK

“…A research nurse obtained venous blood for an enzyme-linked immunosorbent assay (ELISA) HIV test, which is the gold standard diagnostic test. We recorded the time of receipt of HIV test results for each test. …”

Modelling and characterisation of in vivo human skin graft rejection for the investigation of regulatory T cell therapy in vascularised composite allotransplantation by Issa, F

“…It reconstructs the most complex tissue defects effectively, achieving a new functional and cosmetic gold standard. However, VCA transplantation is currently limited by high rates of acute rejection and the need for patients without life-threatening conditions to be maintained on immunosuppression for life. …”

Developing novel blood-stage malaria vaccines by Douglas, AD

“…GIA with antibodies against one antigen, RH5, exceeded that achieved with antibodies against the ‘gold standard’ AMA1 or MSP1 antigens. This antigen’s amino acid sequence is relatively conserved between parasite strains. …”

Development and validation of a quantitative electron microscopy score to assess acute cellular stress in the human exocrine pancreas by Kattner, N, Dyson, N, Bury, Y, Tiniakos, D, White, K, Davey, T, Eliasson, L, Tindale, L, Wagner, BE, Honkanen-Scott, M, Doyle, J, Ploeg, RJ, Shaw, JA, Scott, WE

“…This provides a robust novel tool for gold standard measurement of acute cellular stress in studies evaluating surrogate measures of peri-transplant ischaemia, organ preservation technologies and in samples obtained for detailed pathological examination of underlying pancreatic pathology.…”

Reference ranges for cardiac structure and function in cardiovascular magnetic resonance (CMR) imaging in Caucasians from the UK Biobank population cohort by Petersen, S, Aung, N, Sanghvi, M, Zemrak, F, Fung, K, Miguel Paiva, J, Francis, J, Khanji, M, Lukaschuk, E, Lee, A, Carapella, V, Jin Kim, Y, Leeson, C, Piechnik, S, Neubauer, S

“… <h4>Background</h4> <p>Cardiovascular magnetic resonance (CMR) imaging is the gold standard method for the assessment of cardiac structure and function. …”

The extended recovery ring-stage survival assay provides a superior association with patient clearance half-life and increases throughput by Davis, SZ, Singh, PP, Vendrely, KM, Shoue, DA, Checkley, LA, McDew-White, M, Button-Simons, KA, Cassady, Z, Sievert, MAC, Foster, GJ, Nosten, FH, Anderson, TJC, Ferdig, MT

“…The ring-stage survival assay (RSA) is the current gold standard for in vitro artemisinin resistance phenotyping. …”

Core Outcomes for Colorectal Cancer Surgery: A Consensus Study. by McNair, A, Whistance, R, Forsythe, R, Macefield, R, Rees, J, Pullyblank, A, Avery, K, Brookes, S, Thomas, M, Sylvester, P, Russell, A, Oliver, A, Morton, D, Kennedy, R, Jayne, D, Huxtable, R, Hackett, R, Dutton, S, Coleman, M, Card, M, Brown, J, Blazeby, J

“…Surgical treatment is common, and there is a great need to improve the delivery of such care. The gold standard for evaluating surgery is within well-designed randomized controlled trials (RCTs); however, the impact of RCTs is diminished by a lack of coordinated outcome measurement and reporting. …”

COVID-19 Surveillance in a Primary Care Sentinel Network: In-Pandemic Development of an Application Ontology by de Lusignan, S, Liyanage, H, McGagh, D, Jani, BD, Bauwens, J, Byford, R, Evans, D, Fahey, T, Greenhalgh, T, Jones, N, Mair, FS, Okusi, C, Parimalanathan, V, Pell, JP, Sherlock, J, Tamburis, O, Tripathy, M, Ferreira, F, Williams, J, Hobbs, FDR

“…We developed a coding system–independent COVID-19 case identification algorithm. As there were no gold standard pandemic surveillance ontologies, we conducted a rapid Delphi consensus exercise through the International Medical Informatics Association (IMIA) Primary Health Care Informatics working group and extended networks.…”

MRI visual rating scales in the diagnosis of dementia: evaluation in 184 post-mortem confirmed cases by Harper, L, Fumagalli, G, Barkhof, F, Scheltens, P, O'Brien, J, Bouwman, F, Burton, E, Rohrer, J, Fox, N, Ridgway, G, Schott, J

“…This definitive, multi-centre study uses post-mortem confirmed cases as the gold standard to: (i) assess the reliability of six visual rating scales; (ii) determine their associated pattern of atrophy; (iii) compare their diagnostic value with expert scan assessment; and (iv) assess the accuracy of a machine learning approach based on multiple rating scales to predict underlying pathology. …”

A novel field-based molecular assay to detect validated artemisinin-resistant k13 mutants by Vachot-Ganée, L, Khim, N, Iannello, A, Legrand, E, Kim, S, Eam, R, Khean, C, Ken, M, Ashley, E, Tun, K, Dhorda, M, Nosten, F, Souleymane, I, Blein, S, Pachot, A, Ariey, F, Kaiser, K, Ménard, D

“…Clinical and analytical performances of this tool detecting five of the most frequent and validated k13 mutants (Y493H, I543T, R539T, F446I and C580Y) from dried blood spots (DBS) were compared to the gold standard approach (PCR and Sanger sequencing).</p> <h4>Results</h4> <p>By using the ARMS (amplification-refractory mutation system) strategy, the best multiplexing options were found in 3 separate real-time PCR duplexes (IC as internal control/I543T, C580Y/Y493H and F446I/R539T) with limits of detection ranging from 50 (C580Y) to 6.25 parasites/μL (Y493H). …”

Linkage of the CHHiP randomised controlled trial with primary care data: a study investigating ways of supplementing cancer trials and improving evidence-based practice by Lemanska, A, Byford, RC, Cruickshank, C, Dearnaley, DP, Ferreira, F, Griffin, C, Hall, E, Hinton, W, de Lusignan, S, Sherlock, J, Faithfull, S

“…Background Randomised controlled trials (RCTs) are the gold standard for evidence-based practice. However, RCTs can have limitations. …”

Regenerative agriculture practices and their influence on soil organic carbon and farm productivity in temperate regions by Jordon, M

“…</p> <p>Research for my thesis was divided into four parts. First, I used gold-standard systematic review methods to collate the state of current knowledge and assemble datasets from published studies of management practices forming part of a RA approach. …”

Utility of a lateral flow immunoassay (LFI) to detect Burkholderia pseudomallei in soil samples by Rongkard, P, Hantrakun, V, Dittrich, S, Srilohasin, P, Amornchai, P, Langla, S, Lim, C, Day, N, AuCoin, D, Wuthiekanun, V, Limmathurotsakul, D

“…BACKGROUND: Culture is the gold standard for the detection of environmental B. pseudomallei. …”

Concurrent validity of a low-cost and time-efficient clinical sensory test battery to evaluate somatosensory dysfunction by Zhu, G, Böttger, K, Slater, H, Cook, C, Farrell, S, Hailey, L, Tampin, B, Schmid, A

“…</br> <br><strong>Significance: </strong>Quantitative sensory testing, albeit considered the gold standard to evaluate somatosensory dysfunction, requires expensive equipment, specialized examiner training and substantial time commitment which challenges its use in a clinical setting. …”

Reliability and feasibility of a near patient test for C-reactive protein in primary care. by Hobbs, R, Kenkre, J, Carter, Y, Thorpe, G, Holder, R

“…AIM: A study was set up to evaluate the feasibility of using a novel near patient test for CRP in primary care to validate the results against the laboratory "gold standard' for CRP (Beckman Array) and to compare results with the usual inflammation test used in general practice. …”

Field evaluation of a point-of-care triage test for active tuberculosis (TriageTB) by Richardson, TR, Smith, B, Malherbe, ST, Shaw, JA, Noor, F, MacDonald, C, van der Spuy, GD, Stanley, K, Carstens, A, Fisher, T-L, van Rensburg, I, Flinn, M, Snyders, C, Johnson, I, Fransman, B, Dockrell, H, Thwaites, G, Thuong, NTT, Schacht, C, Mayanja-Kizza, H, Nsereko, M, Tjon Kon Fat, EM, Corstjens, PLAM, Geluk, A, Ruhwald, M, Penn-Nicholson, A, Chegou, NN, Sutherland, J, Walzl, G

“…<p><strong>Methods</strong></p> This observational diagnostic study will evaluate sensitivity and specificity of biomarker-based diagnostic candidates including the MBT and Xpert® TB Fingerstick cartridge compared with a gold-standard composite TB outcome classification defined by symptoms, sputum GeneXpert® Ultra, smear and culture, radiological features, response to TB therapy and presence of an alternative diagnosis. …”

Development and assessment of a genetic and environmental risk model for colorectal cancer by Briggs, S

“…Colonoscopy is the gold standard screening tool but is invasive, expensive, and time consuming. …”

Investigating the use of neurofeedback to promote self-regulation of cortical motor activities as a potential rehabilitation tool post-stroke by Huang, Y

“…</p> <p>The first experiment examined the effect of one fMRI neurofeedback training session on increasing the laterality of cortical motor activity during the movement execution in stroke patients using the “gold standard” of clinical trials. Using a double-blind, sham-controlled, between-subject design, 24 stroke patients underwent a single session of real or sham real-time fMRI neurofeedback training. …”

A comparison of outcome measures used to report clubfoot treatment with the Ponseti method: results from a cohort in Harare, Zimbabwe by Smythe, T, Gova, M, Muzarurwi, R, Foster, A, Lavy, C

“…Diagnostic accuracy was calculated for the five measures and compared to full clinical assessment (gold standard) and whether referral for further intervention was required for re-casting or surgical review.…”