Boosting Replication and Penetration of Oncolytic Adenovirus by Paclitaxel Eradicate Peritoneal Metastasis of Gastric Cancer by Wataru Ishikawa, Satoru Kikuchi, Toshihiro Ogawa, Motoyasu Tabuchi, Hiroshi Tazawa, Shinji Kuroda, Kazuhiro Noma, Masahiko Nishizaki, Shunsuke Kagawa, Yasuo Urata, Toshiyoshi Fujiwara

“…Here, we demonstrate that intraperitoneal (i.p.) administration of a green fluorescence protein (GFP)-expressing attenuated adenovirus with oncolytic potency (OBP-401) synergistically suppressed the peritoneal metastasis of gastric cancer in combination with paclitaxel (PTX). …”
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Rapid Delivery of Nanobodies/VHHs into Living Cells via Expressing In Vitro-Transcribed mRNA by Xuechen Zhou, Rui Hao, Chen Chen, Zhipeng Su, Linhong Zhao, Zhuojuan Luo, Wei Xie

“…We demonstrated that actin-green fluorescent proteins (GFP) and Golgi-GFP can be recognized by the anti-GFP nanobody/VHH, vimentin can be recognized by the anti-vimentin nanobody/VHH, and histone deacetylase 6 (HDAC6) can be recognized by the anti-HDAC6 nanobody/VHH, respectively. …”
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Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), Impair M. smegmatis Growth and Disturb Chromosome Replication by Marcin J. Szafran, Marta Kołodziej, Patrycja Skut, Brahmam Medapi, Agnieszka Domagała, Damian Trojanowski, Jolanta Zakrzewska-Czerwińska, Jolanta Zakrzewska-Czerwińska, Dharmarajan Sriram, Dagmara Jakimowicz, Dagmara Jakimowicz

“…To analyse the effects of amsacrine and its derivatives treatment on cell cycle, we used time-lapse fluorescence microscopy (TLMM) and fusion of the β-subunit of DNA polymerase III with enhanced green fluorescence protein (DnaN-EGFP). We determined that treatment with amsacrine and its derivatives increased the number of DnaN-EGFP complexes and/or prolonged the time of chromosome replication and cell cycle notably. …”
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Adipose tissue-derived microvascular fragments promote lymphangiogenesis in a murine lymphedema model by Florian S Frueh, Laura Gassert, Claudia Scheuer, Andreas Müller, Peter Fries, Anne S Boewe, Emmanuel Ampofo, Claudia E Rübe, Michael D Menger, Matthias W Laschke

“…Secondary lymphedema was induced in the hindlimbs of C57BL/6J mice. Green fluorescence protein (GFP) + MVF were isolated from transgenic C57BL/6Tg (CAG-EGFP)1Osb/J mice, suspended in collagen hydrogel, and injected in the lymphadenectomy defect of wild-type animals. …”
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Examination of Factors Affecting Site-Directed RNA Editing by the MS2-ADAR1 Deaminase System by Md Thoufic Anam Azad, Umme Qulsum, Toshifumi Tsukahara

“…We used the MS2 system and specific guide RNAs to direct ADAR1-DD to target adenosines in the mRNA encoding-enhanced green fluorescence protein. Using this system in transfected HEK-293 cells, we evaluated the effects of changing the length and position of the guide RNA on the efficiency of conversion of amber (TAG) and ochre (TAA) stop codons to tryptophan (TGG) in the target. …”
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Thermo-responsive transfection of DNA complexes with well-defined chitosan terpolymers by Bao, Hongqian, Ping, Yuan, Pan, Yongzheng, Li, Lin, Li, Jun, Gan, Leong Huat

“…The transfection level of TCS and controls was evaluated with COS7 and HeLa cells using two different reporter genes, pRL-CMV encoding luciferase and pEGFP-N1 encoding green fluorescence protein (GFP). The transfection efficiency of one specific terpolymer (TCS4) incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h increased 1–4 fold compared to that incubated at 37 °C for 48 h. …”
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Nanoscopy and multidimensional optical fluorescence microscopy / by Diaspro, Alberto, 1959-

Glycoprotein is enough for sindbis virus-derived DNA vector to express heterogenous genes by Fu Juanjuan, Li Jia, Wang Huanqin, Tang Li, Li Jiangjiao, Zhu Wuyang, Liang Guodong

“…The report gene cassette pVaXJ-EGFP or pV-Gluc expressing enhanced green fluorescence protein (EGFP) or <it>Gaussia </it>luciferase (G.luc) were constructed by cloning <it>EGFP </it>or <it>G.luc </it>gene into pVaXJ. …”
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Tamiflu-resistant but HA-mediated cell-to-cell transmission through apical membranes of cell-associated influenza viruses. by Kotaro Mori, Takahiro Haruyama, Kyosuke Nagata

“…Live cell imaging techniques showed that a recombinant influenza virus, in which the neuraminidase gene was replaced with the green fluorescence protein gene, spreads from an infected cell to adjacent cells forming infected cell clusters. …”
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Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. by Wentao Sun, Jianjun Fang, Qi Yong, Sufang Li, Qingping Xie, Jingbo Yin, Lei Cui

“…Cell tracking assay demonstrated that labeled hASCs remained detectable in the neo-generated tissues 8 weeks post-injection using green fluorescence protein-labeled hASCs. These results indicate that adipose tissue with typical lobule-like structure could be engineered using injectable porous PBLG microspheres loaded with adipogenic-induced hASCs.…”
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Zebrafish (Danio rerio) larvae as a predictive model to study gentamicin-induced structural alterations of the kidney by Jan Stephan Bolten, Christine Tanner, Griffin Rodgers, Georg Schulz, Soledad Levano, Timm Weitkamp, Samuel Waldner, Ramya Deepthi Puligilla, Daniel Bodmer, Bert Müller, Jörg Huwyler

“…We used transgenic zebrafish lines expressing enhanced green fluorescent proteins in the glomerulus to visualize glomerular damage. …”
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Zebrafish (Danio rerio) larvae as a predictive model to study gentamicin-induced structural alterations of the kidney. by Jan Stephan Bolten, Christine Tanner, Griffin Rodgers, Georg Schulz, Soledad Levano, Timm Weitkamp, Samuel Waldner, Ramya Deepthi Puligilla, Daniel Bodmer, Bert Müller, Jörg Huwyler

“…We used transgenic zebrafish lines expressing enhanced green fluorescent proteins in the glomerulus to visualize glomerular damage. …”
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Synthetic mRNA is a more reliable tool for the delivery of DNA-targeting proteins into the cell nucleus than fusion with a protein transduction domain. by Ivan Leontovyc, David Habart, Sarka Loukotova, Lucie Kosinova, Jan Kriz, Frantisek Saudek, Tomas Koblas

“…The system allowed constitutive expression of red fluorescence protein (RFP), while green fluorescence protein (GFP) was expressed only after the genomic action of Cre recombinase. …”
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Characterization of Nrf1b, a novel isoform of the nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes. by Eric K Kwong, Kyung-Mi Kim, Patrick J Penalosa, Jefferson Y Chan

“…Immunoblot analysis of subcellular fractions and imaging of green fluorescence protein (GFP)-tagged Nrf1b demonstrate Nrf1b is constitutively localized to the nucleus. …”
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Mannosylated liposomes for targeted gene delivery by Kong F, Zhou F, Ge L, Liu X, Wang Y.

“…The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression.Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. …”
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Efficient generation of mNeonGreen Plasmodium falciparum reporter lines enables quantitative fitness analysis by Johanna Hoshizaki, Hannah Jagoe, Marcus C. S. Lee

“…Here, we demonstrate an efficient CRISPR-based approach to generate fluorescently labelled parasite lines using mNeonGreen derived from the LanYFP protein in Branchiostoma lanceolatum, which is one of the brightest monomeric green fluorescent proteins identified. Using a positive-selection approach by insertion of an in-frame blasticidin S deaminase marker, we generated a Dd2 reporter line expressing mNeonGreen under the control of the pfpare (P. falciparum Prodrug Activation and Resistance Esterase) locus. …”
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Fluorescent Polymers Conspectus by Guillermo Ahumada, Magdalena Borkowska

“…The Nobel Prizes awarded in 2008 and 2010 for research on the development of green fluorescent proteins and super-resolved fluorescence imaging are proof of this (2014). …”
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Assimilable organic carbon (AOC) determination using GFP-tagged Pseudomonas fluorescens P-17 in water by flow cytometry. by Peng Tang, Jie Wu, Hou Liu, Youcai Liu, Xingding Zhou

“…In this study, a green fluorescence protein (GFP) tagged strain derived of AOC testing strain Pseudomonas fluorescens P-17 (GFP-P17) was generated using Tn5 transposon mutagenesis. …”
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A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling by David L. Prole, Colin W. Taylor

“…Results We generated a toolkit of plasmids encoding nanobodies against red and green fluorescent proteins (RFP and GFP variants), fused to functional modules. …”
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Phosphorylation of SNAP-23 regulates its dynamic membrane association during mast cell exocytosis by Pieu Naskar, Niti Puri

“…To study the role of SNAP-23 phosphorylation in the regulation of exocytosis, green fluorescence protein-tagged wild-type SNAP-23 (GFP-SNAP-23) and its phosphorylation mutants were transfected into rat basophilic leukemia (RBL-2H3) MCs. …”
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