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3841
Interaction of Clostridium perfringens Iota Toxin and Lipolysis-Stimulated Lipoprotein Receptor (LSR)
Published 2018-10-01“…The addition of Ib to LSR-green fluorescence protein (GFP)-transfected cells at 4 °C resulted in colocalization with LSR and Ib on the cell surface. …”
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3842
A Nucleolar Protein, MoRRP8 Is Required for Development and Pathogenicity in the Rice Blast Fungus
Published 2023-09-01“…MoRRP8-GFP (translational fusion of MoRRP8 with green fluorescence protein) co-localizes with a nucleolar marker protein, MoNOP1 fused to red fluorescence protein (RFP), indicating that MoRRP8 is a nucleolar protein. …”
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3843
Two Antibody-Guided Lactic-co-Glycolic Acid-Polyethylenimine (LGA-PEI) Nanoparticle Delivery Systems for Therapeutic Nucleic Acids
Published 2021-08-01“…TNAs used in the current study included tumor suppressor microRNA mimics (miR-198 and miR-520h) and non-coding RNA X-inactive specific transcript (XIST) fragments; green fluorescence protein gene (GFP plasmid DNA) was also used as an example of plasmid DNA. …”
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3844
Enhancing the Activity of a Self-Inducible Promoter in <i>Escherichia coli</i> through Saturation Mutation and High-Throughput Screening
Published 2023-05-01“…These promotors were used as representatives to measure the specific fluorescence and OD<sub>600 nm</sub> dynamics in different media and to confirm their effect on the expression of different proteins, including egfp (enhanced green fluorescence protein) and rfp (red fluorescence protein). …”
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3845
A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle.
Published 2004“…Green fluorescent protein (GFP) is a well-established reporter protein for the examination of biological processes. …”
Journal article -
3846
Induction of pluripotency in human umbilical cord mesenchymal stem cells in feeder layer-free condition
Published 2015“…In the earliest stages, the colonies were green fluorescence protein (GFP)-positive, while upon continuous culture and passage, genuine hiPSC clones expressing GFP were observed. …”
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3847
Inhibition of Monkeypox virus replication by RNA interference
Published 2009-11-01“…Further analysis with wild-type and recombinant MPV expressing green fluorescence protein demonstrated that one of these constructs, siA6-a, was the most potent and inhibited viral replication for up to 7 days at a concentration of 10 nM. …”
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3848
Molecular dissection of mesenchymal-epithelial interactions in the hair follicle.
Published 2005-11-01“…Here, we take a novel multicolor labeling approach, using cell type-specific transgenic expression of red and green fluorescent proteins in combination with immunolabeling of specific antigens, to isolate pure populations of DP and four of its surrounding cell types: dermal fibroblasts, melanocytes, and two different populations of epithelial progenitors (matrix and outer root sheath cells). …”
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3849
Stress Buffering and Longevity Effects of Amber Extract on <i>Caenorhabditis elegans</i> (<i>C. elegans</i>)
Published 2022-06-01“…Survival assays, health span assays, Enzyme-Linked Immunosorbent Assay (ELISA), Stress biomarker detection assays, Green Fluorescence Proteins (GFP), Real Time quantitative PCR (RT-qPCR) and <i>C. elegans</i> mutants were employed to investigate the stress buffering and longevity effect of AE. …”
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3850
Molecular dissection of mesenchymal-epithelial interactions in the hair follicle.
Published 2005-11-01“…Here, we take a novel multicolor labeling approach, using cell type-specific transgenic expression of red and green fluorescent proteins in combination with immunolabeling of specific antigens, to isolate pure populations of DP and four of its surrounding cell types: dermal fibroblasts, melanocytes, and two different populations of epithelial progenitors (matrix and outer root sheath cells). …”
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3851
A Novel Model of Intravital Platelet Imaging Using CD41-ZsGreen1 Transgenic Rats.
Published 2016-01-01“…We generated CD41-ZsGreen1 transgenic rats, in which green fluorescence protein ZsGreen1 was expressed specifically in megakaryocytes and thus platelets. …”
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3852
Effect of Cellular Location of Human Carboxylesterase 2 on CPT-11 Hydrolysis and Anticancer Activity.
Published 2015-01-01“…We linked expression of CE2 and enhanced green fluorescence protein (eGFP) via a foot-and-mouth disease virus 2A (F2A) peptide to facilitate fluorescence-activated cell sorting to achieve similar expression levels of ER-located, secreted or membrane-anchored CE2. …”
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3853
Identifying activated T cells in reconstituted RAG deficient mice using retrovirally transduced Pax5 deficient pro-B cells.
Published 2009-01-01“…We constructed a retroviral double-reporter construct with enhanced green fluorescence protein (EGFP) and a far-red fluorescent protein from Heteractis crispa (HcRed). …”
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3854
Development of transgenic Daphnia magna for visualizing homology-directed repair of DNA
Published 2022-02-01“…Here we developed a transgenic D. magna that expresses green fluorescence protein (GFP) upon HDR occurrence. We utilized the previously established reporter plasmid named DR-GFP that has a mutated eGFP gene (SceGFP) and the tandemly located donor GFP gene fragment (iGFP). …”
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3855
Sesamin Activates Nrf2/Cnc-Dependent Transcription in the Absence of Oxidative Stress in <i>Drosophila</i> Adult Brains
Published 2021-06-01“…Nrf2/Cnc activation was determined using the antioxidant response element, Green Fluorescence Protein reporter, that can monitor Nrf2/Cnc-dependent transcription. …”
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3856
Primary transgenic bovine cells and their rejuvenated cloned equivalents show transgene-specific epigenetic differences.
Published 2012-01-01“…Our transgene contains a puromycin resistance marker gene and an enhanced green fluorescence protein (EGFP) expression cassette, both driven by a strong mammalian promoter, and flanked by loxP sites and sequences from the bovine β-casein locus. …”
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3857
An Easy and Rapid Transformation Protocol for Transient Expression in Cotton Fiber
Published 2022-03-01“…First, we found that exogenous genes could be expressed in cotton fibers via using β-glucuronidase (GUS) and green fluorescence protein (GFP) as reporters. Second, parameters affecting transformation efficiency, including LBA4404 Agrobacterium strain, 3 h infection time, and 2-day incubation time, were determined. …”
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3858
Small interfering RNA targeting m2 gene induces effective and long term inhibition of influenza A virus replication.
Published 2009-01-01“…Using this vector that also expresses enhanced green fluorescence protein (EGFP) as surrogate marker, stable shRNA-expressing cell lines were successfully established and the inhibition efficiencies of rationally designed siRNAs targeting to conserved regions of influenza A virus genome were assessed. …”
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3859
Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer.
Published 2020-01-01“…After transfection, transgenic donor fibroblasts with the hEF1α-enhanced green fluorescence protein (EGFP) transgene were successfully isolated using fluorescence-activated cell sorting (FACS). …”
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3860
Isolation and characterization of new <it>Saccharomyces cerevisiae</it> mutants perturbed in nuclear pore complex assembly
Published 2002-09-01“…We combined a genetic strategy in <it>Saccharomyces cerevisiae</it> with Green Fluorescence Protein (GFP) technology to identify mutants in NPC structure, assembly, and localization. …”
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