Efficient detection of human circulating tumor cells without significant production of false-positive cells by a novel conditionally replicating adenovirus by Fuminori Sakurai, Nobuhiro Narii, Kyoko Tomita, Shinsaku Togo, Kazuhisa Takahashi, Mitsuhiro Machitani, Masashi Tachibana, Masaaki Ouchi, Nobuyoshi Katagiri, Yasuo Urata, Toshiyoshi Fujiwara, Hiroyuki Mizuguchi

“…A novel CTC detection system using a green fluorescence protein (GFP)–expressing conditionally replicating adenovirus (Ad) (rAd-GFP) was recently developed; however, there is concern about the production of false-positive cells (GFP-positive normal blood cells) when using rAd-GFP, particularly at high titers. …”
Get full text

Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve by Ching-Wen Huang, Yuan-Yu Hsueh, Wen-Chin Huang, Shyam Patel, Song Li

“…Methods MVSCs were isolated from rats expressing green fluorescence protein (GFP), expanded, mixed with Matrigel matrix, and loaded into the nerve conduits. …”
Get full text

ARS2 Plays Diverse Roles in DNA Damage Response, Fungal Development, and Pathogenesis in the Plant Pathogenic Fungus Fusarium graminearum by Duc-Cuong Bui, Duc-Cuong Bui, Jung-Eun Kim, Jiyoung Shin, Jae Yun Lim, Gyung Ja Choi, Yin-Won Lee, Jeong-Ah Seo, Hokyoung Son

“…The dominant nuclear localization of FgArs2-green fluorescent proteins (GFP) and abnormal nuclear division in FgARS2 deletion mutant implicated that FgArs2 functions in the nucleus. …”
Get full text

Lipopolysaccharide enhances HSV-1 replication and inflammatory factor release in the ARPE-19 cells by Fang Duan, Weiting Zeng, Yafang Zhang, Dai Li, Kaili Wu

“…Different concentrations of LPS were added. Green fluorescence protein (GFP) of HSVg4 and the infected cell protein 4 (ICP4) expression were observed. …”
Get full text

The Use of Cell-penetrating Peptide for Delivery of Recombinant Transcription Factor DNA into Primary Human Fibroblast by Melinda Remelia, Budiman Bela, Silvia Tri Widyaningtyas, Radiana Dhewayani Antarianto, Nuzli Fahdia Mazfufah, Jeanne Adiwinata Pawitan

“…The transcription factors were delivered into BRED and PAL with recombination of avian leukosis sarcoma virus (ALSV), human immunodeficiency virus (HIV) matrix, and regulator of expression of virion proteins (Rev) (ALMR), tagged with enhanced green fluorescence protein (eGFP). Post-transfection cells were then cultivated with optimized medium. …”
Get full text

A Single Fluorescent Protein-Based Indicator with a Time-Resolved Fluorescence Readout for Precise pH Measurements in the Alkaline Range by Tatiana R. Simonyan, Elena A. Protasova, Anastasia V. Mamontova, Aleksander M. Shakhov, Konstantin A. Lukyanov, Eugene G. Maksimov, Alexey M. Bogdanov

“…Here, we study the pH sensitivity of a green-fluorescence-protein (GFP)-based emitter (EGFP-Y145L/S205V) with the alkaline-shifted chromophore’s pKa and demonstrate that, in the pH range of 7.5–9.0, its fluorescence lifetime changes by a factor of ~3.5 in a quasi-linear manner in mammalian cells. …”
Get full text

Oral vaccination of fish against vibriosis using spore-display technology by Gabriela Gonçalves, Gabriela Gonçalves, Rafaela A. Santos, Rafaela A. Santos, Filipe Coutinho, Neide Pedrosa, Maria Curado, Marina Machado, Benjamin Costas, Benjamin Costas, Lourenço Bonneville, Mónica Serrano, António Paulo Carvalho, António Paulo Carvalho, Patricia Díaz-Rosales, Aires Oliva-Teles, Aires Oliva-Teles, Ana Couto, Cláudia R. Serra, Cláudia R. Serra

“…However, such technology has not been extensively explored against fish bacterial disease.In this study, we used a laboratory strain of B. subtilis, for which a variety of genetic manipulation tools are available, to display at its spores surface either a Vibrio antigenic protein, OmpK, or the green fluorescence protein, GFP. When previously vaccinated by immersion with the OmpK- carrying spores, zebrafish survival upon a bacterial challenge with V. anguillarum and V. parahaemolyticus, increased up to 50 - 90% depending on the pathogen targeted. …”
Get full text

Green Fluorescence Patterns in Closely Related Symbiotic Species of <i>Zanclea</i> (Hydrozoa, Capitata) by Davide Maggioni, Luca Saponari, Davide Seveso, Paolo Galli, Andrea Schiavo, Andrew N. Ostrovsky, Simone Montano

“…Green fluorescence is a common phenomenon in marine invertebrates and is caused by green fluorescent proteins. Many hydrozoan species display fluorescence in their polyps and/or medusa stages, and in a few cases patterns of green fluorescence have been demonstrated to differ between closely related species. …”
Get full text

Applications of fluorescence spectroscopy in protein conformational changes and intermolecular contacts by Fábio Henrique dos Santos Rodrigues, Gonzalo Garcia Delgado, Thyerre Santana da Costa, Ljubica Tasic

“…Therefore, fluorescence anisotropy can be successfully used in protein-protein interaction investigations. Then, green fluorescent proteins (GFPs), photo-transformable fluorescent proteins (FPs) such as photoswitchable and photoconvertible FPs, and those with Large Stokes Shift (LSS) are disclosed in more detail. …”
Get full text

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis by Qinrui Han, Ye Ma, Hao Wang, Yu Dai, Chunhui Chen, Yawei Liu, Linlin Jing, Xuegang Sun

“…Methods SW480 cells with stably expressing enhanced green fluorescence protein were xenografted to BALB/c-nu mice to observe the growth of tumors. …”
Get full text

Binding of Hepatitis B Virus Pre-S1 Domain-Derived Synthetic Myristoylated Peptide to Scavenger Receptor Class B Type 1 with Differential Properties from Sodium Taurocholate Cotran... by Shuji Hinuma, Shun’ichi Kuroda

“…In this study, we examined the binding of Mry47 to SR-B1. (2) Methods: The binding and endocytosis of fluorescence-labeled Myr47 to SR-B1 (and its mutants)-green fluorescence protein (GFP) fusion proteins expressed in HEK293T cells were analyzed using flow cytometry and laser scanning microscopy (LSM). …”
Get full text

Development of Zn2+-controlled expression system for lactic acid bacteria and its application in engineered probiotics by Xiaoning Xu, Lingwen Zhang, Yue Cui, Jian Kong, Tingting Guo

“…To evaluate their possibility to control gene expression in vivo, the sczAst-PczcDst was employed to drive the expression of green fluorescence protein (GFP) gene in L. lactis NZ9000 and S. thermophilus CGMCC7.179, respectively. …”
Get full text

A triazole derivative elicits autophagic clearance of polyglutamine aggregation in neuronal cells by Hsieh CH, Lee LC, Leong WY, Yang TC, Yao CF, Fang K

“…Keywords: autophagic flux, polyglutamine, aggregates clearance, triazole, JNK pathway, neuronal disorders, green fluorescence protein…”
Get full text

Development of a CRISPR/Cas9 system against ruminant animal brucellosis by Garyfalia Karponi, Spyridon K. Kritas, Gina Papadopoulou, Elissavet-Kalliopi Akrioti, Eleni Papanikolaou, Evanthia Petridou

“…We demonstrate a significant decrease in the bacterial load per cell when infected cells are transduced with the RpolA vector and that the number of internalized brucellae per cell remains unaffected when macrophages are transduced with a conventional lentiviral vector expressing the green fluorescence protein, thus underlining the bactericidal effect of our CRISPR/Cas9 system. …”
Get full text

Comparison of In-Frame Deletion, Homology-Directed Repair, and Prime Editing-Based Correction of Duchenne Muscular Dystrophy Mutations by Xiaoying Zhao, Kunli Qu, Benedetta Curci, Huanming Yang, Lars Bolund, Lin Lin, Yonglun Luo

“…The VENUS contains a modified enhanced green fluorescence protein (<i>EGFP</i>) gene, in which expression was restored upon the CRISPR-mediated correction of <i>DMD</i> loss-of-function mutations. …”
Get full text

Type Ⅰ collagen/sodium alginate/hyaluronic acid composite hydrogel for cell loading and 3D culture in vascular tissue engineering by XIE Hang, LIU Chun, HU Hao, WANG Zhiwei

“…Microscopic images of endothelial cells expressing red fluorescent proteins and mesenchymal stem cell expressing green fluorescent proteins were captured with Olympus fluorescence microscope after cell cultured for 0 day, 3 days, 5 days and 7 days in hybrid hydrogel microenvironment, and the images of endothelial cell spheroid growth diffusion after cell cultured for 1 day, 6 days and 9 days. …”
Get full text

Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF165 Neutralization by Tanya A. Camacho-Villegas, María Teresa Mata-González, Walter García-Ubbelohd, Linda Núñez-García, Carolina Elosua, Jorge F. Paniagua-Solis, Alexei F. Licea-Navarro

“…A vNAR called V13, neutralizes VEGF165 cytokine starting at 75 μg/mL in an in vitro assay based on co-culture of normal human dermal fibroblasts (NHDFs) and green fluorescence protein (GFP)-labeled human umbilical vein endothelial cells (HUVECs) cells. …”
Get full text

Mycotoxin Deoxynivalenol Has Different Impacts on Intestinal Barrier and Stem Cells by Its Route of Exposure by Hikaru Hanyu, Yuki Yokoi, Kiminori Nakamura, Tokiyoshi Ayabe, Keisuke Tanaka, Kinuko Uno, Katsuhiro Miyajima, Yuki Saito, Ken Iwatsuki, Makoto Shimizu, Miki Tadaishi, Kazuo Kobayashi-Hattori

“…A time-lapse analysis using enteroids from leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-enhanced green fluorescence protein (EGFP) transgenic mice and 5-ethynyl-2-deoxyuridine (EdU) assay indicated that only the basolateral DON exposure, but not luminal DON exposure, suppressed Lgr5⁺ stem cell count and proliferative cell ratio, respectively. …”
Get full text

Pulmonary exposure to single-walled carbon nanotubes does not affect the early immune response against <it>Toxoplasma gondii</it> by Swedin Linda, Arrighi Romanico, Andersson-Willman Britta, Murray Ashley, Chen Yunying, Karlsson Mikael C I, Georén Susanna Kumlien, Tkach Alexey V, Shvedova Anna A, Fadeel Bengt, Barragan Antonio, Scheynius Annika

“…</p> <p>Methods</p> <p>C57BL/6 mice were pre-exposed by pharyngeal administration of SWCNT (80 + 80 μg/mouse) for two consecutive days followed by intravenous injection with either 1x10³ or 1x10⁴ green fluorescence protein and luciferase-expressing <it>T. gondii</it> tachyzoites. …”
Get full text

Tyrosine Mutation in AAV9 Capsid Improves Gene Transfer to the Mouse Lung by Sabrina V. Martini, Adriana L. Silva, Debora Ferreira, Rafael Rabelo, Felipe M. Ornellas, Karina Gomes, Patricia R.M. Rocco, Hilda Petrs-Silva, Marcelo M. Morales

“…Methods: Eighteen C57BL/6 mice were randomly assigned into three groups: (1) a control group (CTRL) animals underwent intratracheal (i.t.) instillation of saline, (2) the wild-type AAV9 group (WT-AAV9, 1010 vg), and (3) the tyrosine-mutant Y731F AAV9 group (M-AAV9, 1010 vg), which received (i.t.) self-complementary AAV9 vectors containing the DNA sequence of enhanced green fluorescence protein (eGFP). Four weeks after instillation, lung mechanics, morphometry, tissue cellularity, gene expression, inflammatory cytokines, and growth factor expression were analyzed. …”
Get full text