Lack of Evidence for Recipient Precursor Cells Replenishing β-Cells in Transplanted Islets by Yoshiyuki Hamamoto, Tomoyuki Akashi, Akari Inada, Susan Bonner-Weir, Gordon C. Weir M.D.

“…Mouse insulin promoter (MIP)-green fluorescence protein (GFP) mice, which express GFP only in β-cells, or β-actin GFP mice, which express GFP ubiquitously, were used to determine if the recipient-derived cells differentiate into β-cells or other types of endocrine cells. …”
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Subcellular distribution of the V-ATPase complex in plant cells, and <it>in vivo </it>localisation of the 100 kDa subunit VHA-a within the complex by Kluge Christoph, Seidel Thorsten, Bolte Susanne, Sharma Shanti S, Hanitzsch Miriam, Satiat-Jeunemaitre Beatrice, Roß Joachim, Sauer Markus, Golldack Dortje, Dietz Karl-Josef

“…</p> <p>Results</p> <p>To elucidate the presence and function of VHA-a in the plant complex, three approaches were undertaken: (i) co-immunoprecipitation with antibodies directed to epitopes in the N- and C-terminal part of VHA-a, respectively, (ii) immunocytochemistry approach including co-localisation studies with known plant endomembrane markers, and (iii) <it>in vivo</it>-FRET between subunits fused to variants of green fluorescence protein (CFP, YFP) in transfected cells.</p> <p>Conclusions</p> <p>All three sets of results show that V-ATPase contains VHA-a protein that interacts in a specific manner with other subunits. …”
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Intra-tumoral heterogeneity in metastatic potential and survival signaling between iso-clonal HCT116 and HCT116b human colon carcinoma cell lines. by Sanjib Chowdhury, Melanie Ongchin, Elizabeth Sharratt, Ivan Dominguez, Jing Wang, Michael G Brattain, Ashwani Rajput

“…The purpose of this study was to characterize the intra-tumoral phenotypic heterogeneity between two iso-clonal human colon cancer sublines HCT116 and HCT116b on their ability to undergo metastatic colonization and survive under growth factor deprivation stress (GFDS).HCT116 and HCT116b cells were transfected with green fluorescence protein and subcutaneously injected into BALB/c nude male mice. …”
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Overexpression of S100A7 protects LPS-induced mitochondrial dysfunction and stimulates IL-6 and IL-8 in HaCaT cells. by Wenyan Sun, Yan Zheng, Zhuoyang Lu, Yang Cui, Qiong Tian, Shengxiang Xiao, Feng Liu, Jiankang Liu

“…Although S100A7 expression is regulated by various factors in the human epidermis during inflammation, whether S100A7 interacts with mitochondria in keratinocytes is not clear.Our study was designed to investigate whether S100A7 could prohibit mitochondrial dysfunction and stimulate cytokines in cultured normal HaCaT cells treated with LPS.We generated HaCaT cells that constitutively express enhanced green fluorescence protein (EGFP)-S100A7 (S100A7-EGFP) or EGFP alone, as a control. …”
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Multimodality molecular imaging to monitor transplanted stem cells for the treatment of ischemic heart disease. by Zhijun Pei, Xiaoli Lan, Zhen Cheng, Chunxia Qin, Xiaotian Xia, Hui Yuan, Zhiling Ding, Yongxue Zhang

“…The aim of this study was to explore multimodality molecular imaging to monitor transplanted stem cells with a triple-fused reporter gene [TGF; herpes simplex virus type 1 thymidine kinase (HSV1-tk), enhanced green fluorescence protein (eGFP), and firefly luciferase (FLuc)] in acute myocardial infarction rat models.…”
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Involvement of TRPM2 in peripheral nerve injury-induced infiltration of peripheral immune cells into the spinal cord in mouse neuropathic pain model. by Kouichi Isami, Kayo Haraguchi, Kanako So, Kayoko Asakura, Hisashi Shirakawa, Yasuo Mori, Takayuki Nakagawa, Shuji Kaneko

“…Four types of BM chimeric mice were prepared, in which irradiated WT or TRPM2-KO recipient mice were transplanted with either WT-or TRPM2-KO donor mouse-derived green fluorescence protein-positive (GFP(+)) BM cells (TRPM2(BM+/Rec+), TRPM2(BM-/Rec+), TRPM2(BM+/Rec-), and TRPM2(BM-/Rec-) mice). …”
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Identification of Optimal Insertion Site in Recombinant Newcastle Disease Virus (rNDV) Vector Expressing Foreign Gene to Enhance Its Anti-Tumor Effect. by Ziye Pan, Jinjiao He, Lubna M Rasoul, Yunye Liu, Ruixiang Che, Yun Ding, Xiaocheng Guo, Jiarui Yang, Dehua Zou, Hua Zhang, Deshan Li, Hongwei Cao

“…In this study, we inserted the enhanced green fluorescence protein (EGFP) and IL2 genes into four different intergenic regions of the rNDV using reverse genetics technology. …”
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Disease-dependent local IL-10 production ameliorates collagen induced arthritis in mice. by Louise Henningsson, Tove Eneljung, Pernilla Jirholt, Sara Tengvall, Ulf Lidberg, Wim B van den Berg, Fons A van de Loo, Inger Gjertsson

“…Haematopoetic stem cells (HSCs) were transduced with lentiviral particles encoding the IL-10 gene (LNT-IL-10), or a green fluorescence protein (GFP) as control gene (LNT-GFP), driven by the inflammation-dependent IL-1/IL-6 promoter. …”
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Differential effects of silencing crustacean hyperglycemic hormone gene expression on the metabolic profiles of the muscle and hepatopancreas in the crayfish Procambarus clarkii. by Wenfeng Li, Kuo-Hsun Chiu, Yi-Chun Tien, Shih-Fu Tsai, Li-Jane Shih, Chien-Hsun Lee, Jean-Yves Toullec, Chi-Ying Lee

“…Compared to the levels in untreated and saline-injected (SAI) animals, levels of CHH transcript, but not those of molt-inhibiting hormone (a CHH-family peptide), in the eyestalk ganglia of CHH dsRNA-injected (DSI) animals were significantly decreased at 24, 48, and 72 hour post injection (hpi), with concomitant changes in levels of CHH peptide in the sinus gland (a neurohemal organ) and hemolymph. Green fluorescence protein (GFP) dsRNA failed to affect levels of CHH transcript in the eyestalk ganglia of GFP DSI animals. …”
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Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris by Nai-Xin Lin, Rui-Zhen He, Yan Xu, Xiao-Wei Yu

“…Results In this study, we found that the robust mutant not only can express a high level of lipase, but also can express a high level of other heterogeneous proteins (e.g., green fluorescence protein) under methanol induction conditions. …”
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<it>par </it>genes in <it>Mycobacterium bovis </it>and <it>Mycobacterium smegmatis </it>are arranged in an operon transcribed from "SigGC" promoters by Casart Yveth, Gamero Elida, Rivera-Gutierrez Sandra, González-y-Merchand Jorge A, Salazar Leiria

“…The present work focused on the determination of the transcriptional organisation of the ~6 Kb <it>orf60K-parB </it>region of <it>M. bovis </it>BCG and <it>M. smegmatis </it>by primer extension, transcriptional fusions to the green fluorescence protein (GFP) and quantitative RT-PCR.</p> <p>Results</p> <p>The <it>parAB </it>genes were arranged in an operon. …”
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Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion by Sankar P. Chaki, Rola Barhoumi, Gonzalo M. Rivera

“…Confocal and TIRF imaging of cells expressing Green Fluorescence Protein (GFP)  mutant under altered levels of Nck or downstream of kinase 1 (Dok1) was used to evaluate the podosome formation and fluorescent gelatin matrix degradation. …”
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Transferrin-modified nanostructured lipid carriers as multifunctional nanomedicine for codelivery of DNA and doxorubicin by Han Y, Zhang Y, Li D, Chen Y, Sun J, Kong F

“…The aim of this study was to develop surface-modified NLC as multifunctional nanomedicine for codelivery of enhanced green fluorescence protein plasmid (pEGFP) and doxorubicin (DOX). …”
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An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery by Liu YC, Wang T, He FL, Liu Q, Zhang DX, Xiang SL, Su SP, Zhang J

“…The aim of this study was to determine whether protamine sulfate-coated calcium phosphate (PS-CaP) could stabilize particle size and enhance transfection efficiency.Methods: pEGFP-C1 green fluorescence protein was employed as an indicator of transfection efficiency. …”
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Induction of apoptosis by the retinoid inducible growth regulator RIG1 depends on the NC motif in HtTA cervical cancer cells by Lin Su-Ching, Shyu Rong-Yaun, Tsai Fu-Ming, Wu Chang-Chieh, Jiang Shun-Yuan

“…</p> <p>Results</p> <p>Using enhanced green fluorescence protein (EGFP)-tagged RIG1 variants, RIG1 proteins with deletion at the NC domain significantly decreased cell death induced by RIG1, and fusion variants containing only the NC domain significantly induced apoptosis of HtTA cervical cancer cells. …”
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Connexin45 is expressed in vascular smooth muscle but its function remains elusive. by Volker J Schmidt, Alexander Jobs, Julia von Maltzahn, Philipp Wörsdörfer, Klaus Willecke, Cor de Wit

“…Deletion of Cx45 leads concomitantly to the expression of enhanced green fluorescence protein (EGFP) in these mice. Conduction of vasomotor responses was studied in cremasteric arterioles using intravital microscopy and arterial pressure was measured telemetrically. …”
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Tumor-associated macrophages recruit CCR6+ regulatory T cells and promote the development of colorectal cancer via enhancing CCL20 production in mice. by Jinlin Liu, Ning Zhang, Qun Li, Weiwei Zhang, Fang Ke, Qibin Leng, Hong Wang, Jinfei Chen, Honglin Wang

“…Recruitment of FoxP3 green fluorescence protein positive (FoxP3(GFP+)) Treg-cells was assessed using the IVIS Imaging System or immunofluorescence staining. …”
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Parallel-Channel Electrotaxis and Neuron Screening of <em>Caenorhabditis elegans</em> by Khaled Youssef, Daphne Archonta, Terrance Kubiseski, Anurag Tandon, Pouya Rezai

“…Moreover, chemical screening of a PD worm model was shown by exposing the BZ555 strain, expressing green fluorescence protein (GFP) in the dopaminergic neurons (DNs), to 6-hydroxydopamine neurotoxin. …”
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Generation and Characterization of Stable Redox-Reporter Mammalian Cell Lines of Biotechnological Relevance by Karen Perelmuter, Inés Tiscornia, Marcelo A. Comini, Mariela Bollati-Fogolín

“…Recently, different redox-sensitive variants of green fluorescent proteins (e.g., rxYFP, roGFP2, and rxmRuby2) have been engineered and proved suitable to detect, in a non-invasive manner, perturbations in the pool of reduced and oxidized glutathione, the major low molecular mass thiol in mammals. …”
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Aberrant Hedgehog ligands induce progressive pancreatic fibrosis by paracrine activation of myofibroblasts and ductular cells in transgenic zebrafish. by In Hye Jung, Dawoon E Jung, Young Nyun Park, Si Young Song, Seung Woo Park

“…To explore how Hh signaling affects the pancreas, we investigated transgenic phenotypes in zebrafish that over-express either Indian Hh or Sonic Hh along with green fluorescence protein (GFP) to enable real-time observation, or GFP alone as control, at the ptf1a domain. …”
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