-
21
Rational Design of Analyte Channels of the Green Fluorescent Protein for Biosensor Applications
Published 2007-01-01“…<p>A novel solvent-exposed analyte channel, generated by F165G substitution, on the surface of green fluorescent protein (designated His<sub>6</sub>GFPuv/F165G) was successfully discovered by the aid of molecular modeling software (PyMOL) in conjunction with site-directed mutagenesis. …”
Get full text
Article -
22
Lentiviral and Moloney retroviral expression of green fluorescent protein in somatotrophs in vivo.
Published 2013-01-01“…To test this, we first constructed a lentiviral vector, which expresses green fluorescent protein (GFP) under the control of these elements, and injected them into rat pituitaries in situ and in vivo. …”
Get full text
Article -
23
Role of Hydrogen Bonding in Green Fluorescent Protein-like Chromophore Emission
Published 2019-08-01“…Abstract The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. …”
Get full text
Article -
24
An Improved Transformation System for Phytophthora cinnamomi Using Green Fluorescent Protein
Published 2021-07-01“…In this study, we selected ATCC 15400 as a suitable wild-type isolate for PEG/CaCl2 transformation using the green fluorescent protein after screening 11 P. cinnamomi isolates. …”
Get full text
Article -
25
Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP)
Published 2015-06-01Get full text
Article -
26
Green Fluorescent Protein: discover, structure, purification, applications, and future prospects
Published 2023-12-01Subjects: “…green fluorescent protein…”
Get full text
Article -
27
Engineering an efficient and bright split Corynactis californica green fluorescent protein
Published 2021-09-01“…Abstract Split green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. …”
Get full text
Article -
28
Green Fluorescent Protein-Based Viability Assay in a Multiparametric Configuration
Published 2018-06-01Subjects: Get full text
Article -
29
In vivo labelling of Anagallis arvensis L. cells with green fluorescent protein
Published 2014-01-01“…One of the most promising is using the Green Fluorescent Protein (GFP). In our preliminary study we set up the experimental system enabling labelling of Anagallis arvensis cells with this marker. …”
Get full text
Article -
30
Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise
Published 2013-06-01Subjects: Get full text
Article -
31
Computational prediction of the tolerance to amino-acid deletion in green-fluorescent protein.
Published 2017-01-01“…Specifically, we analyze a publicly available dataset of single-amino-acid deletion mutations in enhanced green fluorescent protein (eGFP) to assess how well the functional effect of deletions can be predicted from protein structure. …”
Get full text
Article -
32
Computational Analysis of Chromophore Tripeptides FollowingFusion of Enhanced Green Fluorescent Protein and Cell-FollowingFusion of Enhanced Green Fluorescent Protein and Cell-penetrating Peptides penetrating Peptid
Published 2020-12-01“…In this research, we studied the effect of fusion of new CPPs to the N-terminal of enhanced Green Fluorescent Protein eGFP on the ability of the latter to fluoresce. …”
Get full text
Article -
33
Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids
Published 2017-07-01Subjects: Get full text
Article -
34
Chemical control of excited-state reactivity of the anionic green fluorescent protein chromophore
Published 2024-02-01“…An important example is internal conversion of the anionic green fluorescent protein (GFP) chromophore where non-selective progress along two competing torsional modes (P: phenolate and I: imidazolinone) impairs and enables Z-to-E photoisomerization, respectively. …”
Get full text
Article -
35
Rational design for high bioorthogonal fluorogenicity of tetrazine‐encoded green fluorescent proteins
Published 2022-10-01“…Tetrazine‐encoded green fluorescent proteins (GFPs) show high bioorthogonal reaction rate and genetic encodability but suffer from low fluorogenicity. …”
Get full text
Article -
36
Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
Published 2010-04-01“…<p>Abstract</p> <p>Background</p> <p>The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. …”
Get full text
Article -
37
Generation of a Soluble Form of Human Endoglin Fused to Green Fluorescent Protein
Published 2021-10-01“…Thus, we cloned the extracellular domain of endoglin in the pEGFP-N1 plasmid to generate a fusion protein with green fluorescent protein (GFP), giving rise to pEGFP-N1/Eng.EC. …”
Get full text
Article -
38
A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein
Published 2019-04-01“…In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. …”
Get full text
Article -
39
Erratum: Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise
Published 2013-11-01Subjects: Get full text
Article -
40
Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo
Published 2008-12-01Subjects: “…Green fluorescent protein…”
Get full text
Article