CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy by Zelda Odé, Jose Condori, Nicolas Peterson, Sheng Zhou, Giedre Krenciute

“…As proof-of-principle, we targeted the T-cell receptor alpha constant (<i>TRAC</i>) locus for insertion of a large transgene containing green fluorescence protein (GFP) and interleukin-15 (IL-15). …”
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Dynamic Regulation of GacA in Type III Secretion, Pectinase Gene Expression, Pellicle Formation, and Pathogenicity of Dickeya dadantii (Erwinia chrysanthemi 3937) by Shihui Yang, Quan Peng, Qiu Zhang, Xuan Yi, Chang Jae Choi, Ralph M. Reedy, Amy O. Charkowski, Ching-Hong Yang

“…Reduced transcription of two endo-Pel genes, pelD and pelL, was found in Ech137 using a green fluorescence protein-based fluorescence-activated cell sorter promoter activity assay. …”
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Identification of a Quality Marker (Q-Marker) of Danhong Injection by the Zebrafish Thrombosis Model by Yuqing Qi, Xiaoping Zhao, Hao Liu, Yimin Wang, Chao Zhao, Tao Zhao, Buchang Zhao, Yi Wang

“…The result that DHI prevented the formulation of thrombosis in zebrafish was also validated in the zebrafish thrombosis model with green fluorescence protein (GFP)-labeled hemoglobin. The major components of DHI, namely danshen (DS) and honghua (HH), as well as the major chemical constituents of DHI, also exerted anti-thrombotic effects, among which rosmarinic acid (RA) and p-coumaric acid (pCA) showed moderate anti-thrombotic effects. …”
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Generation of composite Persea americana (Mill.) (avocado) plants: A proof-of-concept-study. by S Ashok Prabhu, Buyani Ndlovu, Juanita Engelbrecht, Noëlani van den Berg

“…The scarred/wounded shoot surfaces were treated with A. rhizogenes strains (K599 or ARqua1) transformed with or without binary plant transformation vectors pRedRootII (DsRed1 marker), pBYR2e1-GFP (GFP- green fluorescence protein marker) or pBINUbiGUSint (GUS- beta-glucuronidase marker) with and without rooting hormone (Dip 'N' Grow) application. …”
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Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells by C. Liu, C.L. Shui, Q. Wang, H. Luo, C.G. Gu

“…Most of the cells in the stably transfected HIF-1α RNAi expression vector pcDNA6.2-GW/EmGFP-miR-siHIF-1α expressed green fluorescence protein (GFP) under fluorescence microscope. pcDNA6.2-GW/EmGFP-miR-siHIF-1α could significantly inhibit HIF-1α gene expression (P<0.05). …”
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LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes’ Shift by Oksana M. Subach, Anna V. Vlaskina, Yulia K. Agapova, Kiryl D. Piatkevich, Maxim V. Patrushev, Valeriya R. Samygina, Fedor V. Subach

“…Red fluorescent proteins with a large Stokes’ shift (LSSRFPs) are genetically encoded and efficiently excited by 488 nm light, allowing simultaneous dual-color one- and two-photon fluorescence imaging and fluorescence correlation spectroscopy in combination with green fluorescent proteins FPs. Recently, based on the conventional bright mScarlet RFP, we developed the LSSRFP LSSmScarlet. …”
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Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice by Zhenxiong Ye, Wenfeng Lu, Lei Liang, Min Tang, Yunfeng Wang, Zhen Li, Heping Zeng, Aili Wang, Moubin Lin, Lei Huang, Hui Wang, Hai Hu

“…Methods Carbon tetrachloride (CCl4) was used to establish a mouse liver cirrhosis model. Enhanced green fluorescence protein (EGFP) and hepatocyte nuclear factor-4α (HNF-4α) overexpression adenoviruses were used to modify MSCs. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…Then, CHO­S/FRT cells, which contain the FRT­site for homologous recombination and are able to produce green fluorescence protein GFP, were transfected with the constructed plasmid. …”
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Genetic Transformation of Recalcitrant Upland Switchgrass Using Morphogenic Genes by Nuoya Xu, Nuoya Xu, Nuoya Xu, Minjeong Kang, Minjeong Kang, Minjeong Kang, Jacob D. Zobrist, Jacob D. Zobrist, Jacob D. Zobrist, Kan Wang, Kan Wang, Shui-zhang Fei, Shui-zhang Fei

“…Two auxotrophic Agrobacterium strains, LBA4404Thy- and EHA105Thy-, each harboring the same construct containing ZmBbm, ZmWus2, and a green fluorescence protein (GFP) gene, ZsGreen1, were used to infect immature leaf segments derived from in vitro grown seedlings. …”
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Interspecific Potato Breeding Lines Display Differential Colonization Patterns and Induced Defense Responses after Ralstonia solanacearum Infection by Virginia Ferreira, María J. Pianzzola, Francisco L. Vilaró, Guillermo A. Galván, María L. Tondo, María L. Tondo, María V. Rodriguez, Elena G. Orellano, Elena G. Orellano, Marc Valls, Marc Valls, María I. Siri

“…A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. …”
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Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutro... by Jassim M. Al-Hassan, Aleksander Hinek, Waleed M. Renno, Yanting Wang, Yuan Fang Liu, Rui Guan, Xiao-Yen Wen, Xiao-Yen Wen, Michael L. Litvack, Andras Lindenmaier, Mohammad Afzal, Bincy Paul, Sosamma Oommen, Divya Nair, Jijin Kumar, Meraj A. Khan, Nades Palaniyar, Nades Palaniyar, Cecil Pace-Asciak, Cecil Pace-Asciak

“…Ft increased the production and extracellular deposition of collagen and fibronectin, ex vivo, iii) Ft and its subcomponents, pure F6 and S5, also promoted human dermal fibroblast migration into the scratch wound gaps, ex vivo, iv) Ft, F6, and S5 promoted the recruitment of neutrophils (Green fluorescence protein labeled) to the site of injury in the transected tailfins of transgenic zebrafish, in vivo, v) Ft, but not F6 or S5, promoted the regeneration of tissues at the wound site in the transgenic zebrafish tailfin, in vivo. …”
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Characterization of the Aspergillus fumigatus detoxification systems for reactive nitrogen intermediates and their impact on virulence by Katrin eLapp, Martin eVödisch, Kristin eKroll, Maria eStrassburger, Olaf eKniemeyer, Olaf eKniemeyer, Olaf eKniemeyer, Thorsten eHeinekamp, Thorsten eHeinekamp, Axel A Brakhage, Axel A Brakhage

“…By generating fusions with the green fluorescence protein, we showed that both FhpA-eGFP and GnoA-eGFP were located in the cytoplasm of all A. fumigatus morphotypes, from conidia to hyphae, whereas FhpB-eGFP was localized in mitochondria. …”
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Amburana cearensis seed extract stimulates astrocyte glutamate homeostatic mechanisms in hippocampal brain slices and protects oligodendrocytes against ischemia by Rafael Short Ferreira, Paulo Roberto Ribeiro, Juliana Helena Castro e Silva, Juliana Bender Hoppe, Monique Marylin Alves de Almeida, Beatriz Correia de Lima Ferreira, Gustavo Borges Andrade, Suzana Braga de Souza, Luzimar Gonzaga Ferdandez, Maria de Fátima Dias Costa, Christianne Gazzana Salbego, Andrea Domenico Rivera, Aline Longoni, Adriano Martimbianco de Assis, Francesca Pieropan, José Cláudio Fonseca Moreira, Silvia Lima Costa, Arthur Morgan Butt, Victor Diogenes Amaral da Silva

“…Next, we directly examined astrocytes using transgenic mice in which glial fibrillary acidic protein (GFAP) drives expression of enhanced green fluorescence protein (EGFP) and show that glutamate excitotoxicity caused a decrease in GFAP-EGFP and that EDAC protected against this loss. …”
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Effects of Mini-Dystrophin on Dystrophin-Deficient, Human Skeletal Muscle-Derived Cells by Jinhong Meng, John Counsell, Jennifer E. Morgan

“…Methods: Skeletal muscle-derived cells from a DMD patient were transduced with lentivirus coding for mini-dystrophins (R3–R13 spectrin-like repeats (ΔR3R13) or hinge2 to spectrin-like repeats R23 (ΔH2R23)) with EGFP (enhanced green fluorescence protein) fused to the C-terminus, driven by a constitutive promoter, spleen focus-forming virus (SFFV). …”
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Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells by Chan Shih-Hung, Kikkawa Ushio, Matsuzaki Hidenori, Chen Jyh-Hong, Chang Wen-Chang

“…Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. …”
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Clearance of peripheral nerve misfolded mutant protein by infiltrated macrophages correlates with motor neuron disease progression by Wataru Shiraishi, Ryo Yamasaki, Yu Hashimoto, Senri Ko, Yuko Kobayakawa, Noriko Isobe, Takuya Matsushita, Jun-ichi Kira

“…To clarify the CCR2 function in the model, we generated SOD1 G93A/CCR2 Red fluorescence protein (RFP)/Wild type (WT) /CX3CR1 Green fluorescence protein (GFP)/WT -Tg mice, which heterozygously express CCR2-RFP and CX3CR1-GFP, and SOD1 G93A/CCR2 RFP/RFP-Tg mice, which lack CCR2 protein expression and present with a CCR2-deficient phenotype. …”
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Pigeon RIG-I Function in Innate Immunity against H9N2 IAV and IBDV by Wenping Xu, Qiang Shao, Yunlong Zang, Qiang Guo, Yongchao Zhang, Zandong Li

“…The exogenous expression of enhanced green fluorescence protein (EGFP)-tagged pigeon RIG-I and caspase activation and recruitment domains (CARDs), strongly induced antiviral gene (IFN-β, Mx, and PKR) mRNA synthesis, decreased viral gene (M gene and VP2) mRNA expression, and reduced the viral titers of ZB07 and IBDV TS/CJ-801 virus strains in chicken DF-1 cells, but not in 293T cells. …”
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An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives by Liang X, Ren X, Liu Z, Liu Y, Wang J, Wang J, Zhang LM, Deng DY, Quan D, Yang L

“…Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group. …”
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Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection by Anan Huang, Yue Liu, Xin Qi, Shang Chen, Haoyan Huang, Jun Zhang, Zhibo Han, Zhong-Chao Han, Zongjin Li

“…Methods MSCs were isolated from human placenta tissue, and DF-MSCs or Gluc-MSCs were generated by transduced with firefly luciferase (Fluc)/enhanced green fluorescence protein (eGFP) or Gaussia luciferase (Gluc) lactadherin fusion protein. …”
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Effect of SMYD3 on biological behavior and H3K4 methylation in bladder cancer by Wu X, Xu Q, Chen P, Yu C, Ye L, Huang C, Li T

“…We also constructed green fluorescence protein lentiviral vector using the gene short hairpin RNA (shRNA) system. …”
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