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81
Mammalian cell lines expressing functional RNA polymerase II tagged with the green fluorescent protein.
Published 2000“…We wished to study these different complexes in living cells, so we generated cell lines expressing the largest, catalytic, subunit of the polymerase tagged with the green fluorescent protein. The tagged enzyme complements a deficiency in tsTM4 cells that have a temperature-sensitive mutation in the largest subunit. …”
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82
Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.
Published 2008Subjects: Get full text
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83
Displaying Of Green Fluorescent Protein On A Recombinant Bacteriophage Infecting Escherichia Coll Atcc 11303
Published 2017“…The technology was applied to display a green fluorescence protein (GFP) on tail protein of Escherichia Phage YD-2008.S to create GFP labelled recombinant bacteriophage.…”
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Thesis -
84
Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
Published 2012“…An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. …”
Article -
85
Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research.
Published 2008-01-01“…<h4>Methodology/principal findings</h4>Here we report a novel multifunctional green fluorescent protein (mfGFP) tag which was engineered by inserting multiple peptide tags, i.e., octa-histidine (8xHis), streptavidin-binding peptide (SBP), and c-Myc tag, in tandem into a loop of GFP. …”
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86
Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein
Published 2024-01-01“…Introducing 3-aminotyrosine (aY), a noncanonical amino acid (ncAA), into green fluorescent protein (GFP)-like chromophores shows promise for achieving red-shifted fluorescence. …”
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Article -
87
Transient Expression of Green Fluorescent Protein in Rice Calluses : Optimization of Parameters for Helios Gene Gun Device
Published 2008-01-01Subjects: Get full text
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88
Silica Nanoparticles for Intracellular Protein Delivery: a Novel Synthesis Approach Using Green Fluorescent Protein
Published 2017-09-01Subjects: Get full text
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89
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Green fluorescent protein-like pigments optimise the internal light environment in symbiotic reef-building corals
Published 2022-07-01“…Pigments homologous to the green fluorescent protein (GFP) have been proposed to fine-tune the internal light microclimate of corals, facilitating photoacclimation of photosynthetic coral symbionts (Symbiodiniaceae) to life in different reef habitats and environmental conditions. …”
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Article -
91
Green Fluorescent Protein is a Suitable Reporter of Tumor Hypoxia Despite an Oxygen Requirement for Chromophore Formation
Published 2001-01-01Subjects: “…green fluorescent protein…”
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92
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Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in <it>Pichia pastoris</it>
Published 2007-05-01“…In this context, the green fluorescence protein (GFP) has been proposed as a molecular reporter when fused to target proteins to study their subcellular localization or secretion behaviour. …”
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Establishment of Green Fluorescent Protein and Firefly Luciferase Expressing Mouse Primary Macrophages for In Vivo Bioluminescence Imaging.
Published 2015-01-01“…Bioluminescence imaging (BLI) with green fluorescent protein (GFP) and firefly luciferase (FLUC) labelled autologous reporter macrophages could potentially offer a powerful tool to study macrophage biology, but this approach has been hindered by the relative difficulty of efficient gene transfer into primary macrophages. …”
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Article -
96
Evaluation of phagocytosis in human neutrophils using enhanced green fluorescent protein (EGFP) expressing E. coli
Published 2022-03-01“…Materials and Methods: An EGFP (Enhanced green fluorescent protein) sequence was cloned into a pColdI expression vector. …”
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Effect of imidazole concentration and type of metal ion on the purification of recombinant green fluorescent protein using an affinity chromatography
Published 2014“…This paper presents the effect of imidazole concentration and type of metal ion studies on the purification of recombinant green fluorescent protein (GFP) using an affinity chromatography. …”
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Undergraduates Project Papers -
99
Electrophoretic purification of recombinant green fluorescent protein from intact escherichia coli cells in continuous buffer system
Published 2014“…Green fluorescent protein (GFP) is a protein that consists of 27 kDa protein of 238 amino acid residues. …”
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Undergraduates Project Papers -
100
Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase
Published 2021-04-01“…Herein, we report on the first development of a stable transgenic T. equi line expressing enhanced green fluorescent protein/blasticidin S deaminase (eGFP/BSD). …”
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Article