Small RNA pathways are present and functional in the angiosperm male gametophyte. ανά Grant-Downton, R, Hafidh, S, Twell, D, Dickinson, H

“…Surprisingly, very little is known of their presence and function in the male haploid phase of plant development (male gametophyte/pollen grain), where dramatic cell fate changes leading to gametogenesis occur over just two mitotic divisions. …”

Identification of an MCM4 homologue expressed specifically in the sexual stage of Plasmodium falciparum. ανά Li, J, Cox, L

“…Southern blotting of genomic DNA digests and chromosomal separations showed that the Pfmcm4 gene is present as a single copy per haploid genome and is located on chromosome 13. A 4000-nucleotide transcript of this gene is expressed specifically in the sexual erythrocytic stage, indicating that PfMCM4 may be involved in gametogenesis in which DNA is quickly replicated.…”

Rapid genotype imputation from sequence with reference panels ανά Davies, RW, Kucka, M, Su, D, Shi, S, Flanaghan, M, Cunniff, CM, Chan, YF, Myers, S

“…QUILT employs Gibbs sampling to partition reads into maternal and paternal sets, facilitating rapid haploid imputation using large reference panels. We show this partitioning to be accurate over many megabases, enabling highly accurate imputation close to theoretical limits and outperforming existing methods. …”

An investigation of the origin and significance of bilateral symmetry of the pronuclear zygote in the mouse. ανά Gardner, R, Davies, T

“…Moreover, both the axis of polarity and bilateral plane of the blastocyst bore a consistent relationship to the zygote's bilateral plane. Haploid parthenotes also exhibited bilateral symmetry, although in the absence of fertilization, first cleavage was less consistently orthogonal to the bilateral plane. …”

Aneuploidy triggers a TFEB-mediated lysosomal stress response ανά Santaguida, Stefano, Amon, Angelika B

“…Aneuploidy, defined as an alteration in chromosome number that is not a multiple of the haploid complement, severely affects cellular physiology. …”
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Aneuploidy: implications for protein homeostasis and disease ανά Oromendia, Ana Belen, Amon, Angelika B

“…It has long been appreciated that aneuploidy – in which cells possess a karyotype that is not a multiple of the haploid complement – has a substantial impact on human health, but its effects at the subcellular level have only recently become a focus of investigation. …”
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EMBRIOGENESIS MIKROSPORA PADA TANAMAN PADI (Oryza sativa L.) HIBRIDA (Menthik X Cempo merah) ανά , Andin Vita Amalia, , Dr.rer.nat Ari Indrianto, SU.

“…The efforts to shorten the time of breeding and to obtain excellent hybrid in one generation is by microspore culture techniques to produce double haploid plants through the process of embryogenesis. …”

Male influence on sex allocation in the parasitoid wasp Nasonia vitripennis ανά Shuker, D, Sykes, E, Browning, L, Beukeboom, L, West, SA

“…However, it is often assumed that females have substantial control over sex allocation decisions, and this is particularly true in haplodiploid insects, in which females apparently determine sex by deciding whether to fertilise an egg (and produce a diploid daughter) or not (and produce a haploid son). Mechanisms by which males may influence sex allocation are not so straightforward, and their potential influence on sex ratios has been somewhat neglected. …”

Quantification of transmission risk in a male patient with a FLNB mosaic mutation causing Larsen syndrome: Implications for genetic counseling in postzygotic mosaicism cases ανά Bernkopf, M, Hunt, D, Koelling, N, Morgan, T, Collins, A, Fairhurst, J, Robertson, S, Douglas, A, Goriely, A

“…The mutation was detectable in all tissues tested, at levels ranging from 7-10% (mutation present in ~20% of diploid somatic cells and 7% of haploid sperm), demonstrating the involvement of both somatic and gonadal lineages in this patient. …”

The OKRA leaf shape mutation in cotton is active in all cell layers of the leaf. ανά Dolan, L, Poethig, R

“…Chimeras containing L2O and wild-type tissue were generated using Semigamy (Se), a mutation that blocks syngamy during fertilization and produces haploid maternal/paternal chimeral progeny at low frequency. …”

Characterisation of a sexual stage-specific gene encoding ORC1 homologue in the human malaria parasite Plasmodium falciparum. ανά Li, J, Cox, L

“…Southern blot analyses show that the Pforc1 gene is present as a single copy per haploid genome and is located on chromosome 12. A 5600 nucleotide transcript of this gene is expressed predominantly in the sexual erythrocytic stage, indicating that PfORC1 may be involved in gametogenesis during which DNA is quickly replicated.…”

Marchantia polymorpha as a testbed species for metabolic engineering ανά Lindström Battle, A

“…<em>Marchantia polymorpha</em>, with its haploid-dominant lifecycle and ease of genetic manipulation, has emerged as a promising candidate for exploring plant metabolic engineering. …”

Retinoic Acid and Germ Cell Development in the Ovary and Testis ανά Endo, Tsutomu, Mikedis, Maria M., Nicholls, Peter K., Page, David C, de Rooij, Dirk G.

“…After birth, male germ cells resume proliferation and undergo a transition to spermatogonia, which are destined to develop into haploid spermatozoa via spermatogenesis. Recent findings indicate that RA levels change periodically in adult testes to direct not only meiotic initiation, but also other key developmental transitions to ensure that spermatogenesis is precisely organized for the prodigious output of sperm. …”
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A role for the Speedy gene family in the early stages of mammalian meiosis ανά Lopez-Molina, Javier

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Kinetochore asymmetry defines a single yeast lineage ανά Thorpe, Peter H., Bruno, Joanne H., Rothstein, Rodney

“…This phenotype is not seen in vegetatively dividing haploid or diploid cells. This kinetochore asymmetry suggests a mechanism for the selective segregation of sister centromeres to daughter cells to establish different cell lineages or fates. …”
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Auxin promotes the transition from chloronema to caulonema in moss protonema by positively regulating PpRSL1and PpRSL2 in Physcomitrella patens ανά Jang, G, Dolan, L

“…• Protonemata are multicellular filamentous networks that develop following the germination of a haploid moss spore and comprise two different cell types - chloronema and caulonema. …”

Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. ανά Katis, V, Lipp, J, Imre, R, Bogdanova, A, Okaz, E, Habermann, B, Mechtler, K, Nasmyth, K, Zachariae, W

“…During meiosis, two rounds of chromosome segregation after a single round of DNA replication produce haploid gametes from diploid precursors. At meiosis I, maternal and paternal kinetochores are pulled toward opposite poles, and chiasmata holding bivalent chromosomes together are resolved by cleavage of cohesin's alpha-kleisin subunit (Rec8) along chromosome arms. …”

Close kinship within multiple-genotype malaria parasite infections. ανά Nkhoma, S, Nair, S, Cheeseman, I, Rohr-Allegrini, C, Singlam, S, Nosten, F, Anderson, T

“…Instead, the observed kinship patterns may be explained by inoculation of multiple related haploid sporozoites from single mosquito bites, by immune suppression of parasite subpopulations within infections, and serial transmission of related parasites between people. …”

Auxin promotes the transition from chloronema to caulonema in moss protonema by positively regulating PpRSL1and PpRSL2 in Physcomitrella patens. ανά Jang, G, Dolan, L

“…Protonemata are multicellular filamentous networks that develop following the germination of a haploid moss spore and comprise two different cell types - chloronema and caulonema. …”

Preparation of high-quality next-generation sequencing libraries from picogram quantities of target DNA. ανά Parkinson, N, Maslau, S, Ferneyhough, B, Zhang, G, Gregory, L, Buck, D, Ragoussis, J, Ponting, C, Fischer, MD

“…This finding was confirmed with exhaustive resequencing of a mouse library constructed from 20 pg of gDNA input (about seven haploid genomes) resulting in ∼0.4-fold statistical coverage of uniquely mapped fragments. …”