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Ruler Arrays Reveal Haploid Genomic Structural Variation
Published 2012“…In an empirical test between two closely related haploid strains of yeast ruler arrays detected 78% of the structural variants larger than 100 bp.…”
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Plant Y chromosome degeneration is retarded by haploid purifying selection.
Published 2011“…Our results suggest that plants represent an exception to this rule as a result of their unique life-cycle that includes alteration of diploid and haploid generations and widespread haploid expression of genes in plant gametophytes [3]. …”
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Plant y chromosome degeneration is retarded by haploid purifying selection
Published 2011“…Our results suggest that plants represent an exception to this rule as a result of their unique life-cycle that includes alteration of diploid and haploid generations and widespread haploid expression of genes in plant gametophytes [3]. …”
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Haploid reporter screens aimed at identification of NF-kappaB regulators
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Functional genomics and compound mode-of-action screening in haploid human cells
Published 2017Subjects:Thesis -
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A reversible gene trap collection empowers haploid genetics in human cells.
Published 2013“…Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. …”
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Genome-wide genetic screening with chemically-mutagenized haploid embryonic stem cells
Published 2016“…Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.…”
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Haploid genetic screening approach towards identifying novel regulators of immune checkpoints
Published 2021“…This thesis describes the use of a haploid genetic screening approach to uncover regulatory mechanisms of several immune checkpoint proteins expressed on cancer cells.…”
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Performance of crosses and development of doubled haploid lines in wheat (Triticum aestivum L.) for salt tolerance
Published 2018“…Thirty-nine lines were fertile and their seeds were grown for developing salt tolerant doubled haploid lines. Haploid plant's chromosomes were doubled under colchicine treatment (0.2%) with dimethyl sulfoxide (DMSO) (2%) and 2-3 drops of Tween-20 for 4 hours at room temperature. …”
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Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line.
Published 2014“…Near-haploid human cell lines are instrumental for genetic screens and genome engineering as gene inactivation is greatly facilitated by the absence of a second gene copy. …”
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Detection of oil palm haploids using stomata counting, flow cytometry, microsatellite and genomic in situ hybridization techniques
Published 2016“…However, the reliable method to screen for oil palm haploid and doubled haploid is yet to be reported. …”
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A Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κB
Published 2013“…The development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. …”
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INDUKSI HAPLOID GANDA PADA PADI JAPONICA (Oryza sativa L. ssp. japonica) , INDICA (Oryza sativa L. ssp. indica) DAN HIBRIDA JAPONICA � INDICA
Published 2013“…The results showed that genotype affect double haploid induction through anther culture. Crossing between the two genotypes can give positive results in increasing response in anther culture of F1. …”
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Descriptive study on the origin and evolution of beta- defensins copy number variable gene using multigenerational family pedigrees
Published 2019“…Moreover, DEFB copy number was successfully quantified in both diploid and haploid copies.…”
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Global gene disruption in human cells to assign genes to phenotypes by deep sequencing.
Published 2011“…Insertional mutagenesis in a haploid background can disrupt gene function. We extend our earlier work by using a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes. …”
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Microtubules and actin filaments direct nuclear movement during the polarisation of Marchantia spore cells
Published 2024“…The multicellular haploid stage of land plants develops from a single haploid cell produced by meiosis – the spore. …”
Journal article