ABCG2 Is Overexpressed on Red Blood Cells in Ph-Negative Myeloproliferative Neoplasms and Potentiates Ruxolitinib-Induced Apoptosis by Ralfs Buks, Mégane Brusson, Sylvie Cochet, Tatiana Galochkina, Bruno Cassinat, Ivan Nemazanyy, Thierry Peyrard, Jean-Jacques Kiladjian, Alexandre G. de Brevern, Slim Azouzi, Wassim El Nemer

“…Myeloproliferative neoplasms (MPNs) are a group of disorders characterized by clonal expansion of abnormal hematopoietic stem cells leading to hyperproliferation of one or more myeloid lineages. …”
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Vaccination against SARS-COV‑2 in oncology by A. A. Polyakov, V. V. Lunin, F. M. Abbaysbeyli, O. L. Timofeeva, V. B. Larionova, P. A. Zeynalova, A. A. Fedenko

“…The review presents current recommendations for vaccination against SARS-COV‑2 in patients with solid tumors, hematological malignancies, recipients of hematopoietic stem cells and cell therapy.Conclusion. To date, groups at increased risk of infection with the new coronavirus have been identified. …”
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PREDICTION OF POST-TRANSPLANT RELAPSE IN THE MYELODYSPLASTIC SYNDROMES VIA EVALUATION OF STEM CELLS by S. Chung, B. Kroger, Y. Huang, A. Son, P. Carlsgaard, L. Pop, R. Vittrup, F. Kalkan, A. Cherukuri, D. Sallman, R. Tamari, C. Gurnari, J. Maciejewski, Y. Madanat

“…Methods: To test our hypothesis that hematopoietic stem cells (HSCs) drive post-transplant relapse, we developed a protocol to perform ECS on as few as <25 HSCs. …”
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Bone Marrow Adipose Tissue Is Not Required for Reconstitution of the Immune System Following Irradiation in Male Mice by Jessica A. Keune, Carmen P. Wong, Adam J. Branscum, Scott A. Menn, Urszula T. Iwaniec, Russell T. Turner

“…Irradiation was followed by adoptive transfer of 1000 purified WT hematopoietic stem cells (HSCs). The extent of immune reconstitution in blood, bone marrow, and lymph nodes in the irradiated mice was determined using HSCs from green fluorescent protein (GFP)-expressing mice. …”
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Pretransplant BK Virus-Specific T-Cell-Mediated Immunity and Serotype Specific Antibodies May Have Utility in Identifying Patients at Risk of BK Virus-Associated Haemorrhagic Cysti... by Markéta Šťastná-Marková, Eva Hamšíková, Petr Hainz, Petr Hubáček, Marie Kroutilová, Jitka Kryštofová, Viera Ludvíková, Jan Musil, Pavla Pecherková, Martina Saláková, Vojtěch Šroller, Jan Vydra, Šárka Němečková

“…In some immunocompromised persons after transplantation of hematopoietic stem cells (HSCT), the BKPyV high-rate replication is associated with haemorrhagic cystitis (HC). …”
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Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing by Weijian Liu, Wei Chen, Mao Xie, Chao Chen, Zengwu Shao, Yiran Zhang, Haiyue Zhao, Qingcheng Song, Hongzhi Hu, Xin Xing, Xianyi Cai, Xiangtian Deng, Xinyan Li, Peng Wang, Guohui Liu, Liming Xiong, Xiao Lv, Yingze Zhang

“…TBI-induced hypersensitivity of adrenergic signaling promotes the proliferation of bone marrow hematopoietic stem cells (HSCs) and swiftly skews HSCs toward anti-inflammation myeloid cells within 14 days, which favor fracture healing. …”
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Case report: CD38-directed CAR-T cell therapy: A novel immunotherapy targeting CD38- positive blasts overcomes TKI and chemotherapy resistance of myeloid chronic myeloid leukemia i... by Qingya Cui, Qingya Cui, Peiqi Liang, Peiqi Liang, Haiping Dai, Haiping Dai, Wei Cui, Wei Cui, Mengjie Cai, Mengjie Cai, Zixuan Ding, Zixuan Ding, Qinfen Ma, Qinfen Ma, Jia Yin, Jia Yin, Zheng Li, Zheng Li, Sining Liu, Sining Liu, Liqing Kang, Liqing Kang, Li Yao, Li Yao, Jiannong Cen, Jiannong Cen, Hongjie Shen, Hongjie Shen, Mingqing Zhu, Mingqing Zhu, Lei Yu, Lei Yu, Depei Wu, Depei Wu, Xiaowen Tang, Xiaowen Tang

“…CD38 is known to be expressed on most (acute myeloid leukemia) AML cells, and its lack of expression on hematopoietic stem cells renders it as a potential therapeutic target for myeloid CML-BP. …”
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Human-Immune-System (HIS) humanized mouse model (DRAGA: HLA-A2.HLA-DR4.Rag1KO.IL-2RγcKO.NOD) for COVID-19 by Teodor-D. Brumeanu, Pooja Vir, Ahmad Faisal Karim, Swagata Kar, Dalia Benetiene, Megan Lok, Jack Greenhouse, Tammy Putmon-Taylor, Christopher Kitajewski, Kevin K. Chung, Kathleen P. Pratt, Sofia A. Casares

“…When infused with human hematopoietic stem cells from cord blood reconstitute a functional human immune system, as well as human epi/endothelial cells in lung and upper respiratory airways expressing the human ACE2 receptor for SARS-CoV-2. …”
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CD7-positive leukemic blasts with DNMT3A mutations predict poor prognosis in patients with acute myeloid leukemia by Yanliang Bai, Xiaobai Sun, Mengyi Li, Xiaona Niu, Weijie Cao, Junwei Niu, Xingjun Xiao, Yuqing Chen, Kai Sun, Kai Sun

“…BackgroundDNMT3A mutations can be detected in premalignant hematopoietic stem cells and are primarily associated with clonal hematopoiesis of indeterminate potential; however, current evidence does not support assigning them to a distinct European Leukemia Net (ELN) prognostic risk stratification. …”
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Occurrence of L1M Elements in Chromosomal Rearrangements Associated to Chronic Myeloid Leukemia (CML): Insights from Patient-Specific Breakpoints Characterization by Alberto L’Abbate, Vittoria Moretti, Ester Pungolino, Giovanni Micheloni, Roberto Valli, Annalisa Frattini, Matteo Barcella, Francesco Acquati, Rolland A Reinbold, Lucy Costantino, Fulvio Ferrara, Alessandra Trojani, Mario Ventura, Giovanni Porta, Roberto Cairoli

“…Chronic myeloid leukemia (CML) is a rare myeloproliferative disorder caused by the reciprocal translocation t(9;22)(q34;q11) in hematopoietic stem cells (HSCs). This chromosomal translocation results in the formation of an extra-short chromosome 22, called a Philadelphia chromosome (Ph), containing the <i>BCR-ABL1</i> fusion gene responsible for the expression of a constitutively active tyrosine kinase that causes uncontrolled growth and replication of leukemic cells. …”
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<i>Tsc1</i> Regulates the Proliferation Capacity of Bone-Marrow Derived Mesenchymal Stem Cells by Maria V. Guijarro, Laura S. Danielson, Marta Cañamero, Akbar Nawab, Carolina Abrahan, Eva Hernando, Glyn D. Palmer

“…TSC1 modulates self-renewal and differentiation in hematopoietic stem cells; however, its effects on mesenchymal stem cells (MSCs) are unknown. …”
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Genome editing for sickle cell disease: still time to correct? by Giulia Ceglie, Giulia Ceglie, Marco Lecis, Marco Lecis, Marco Lecis, Gabriele Canciani, Gabriele Canciani, Mattia Algeri, Giacomo Frati

“…The monogenic nature of the disease and the availability of autologous hematopoietic stem cells (HSCs) make this disorder an ideal candidate for gene modification strategies. …”
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Proximity Ligation Assay Detection of Protein–DNA Interactions—Is There a Link between Heme Oxygenase-1 and G-quadruplexes? by Wojciech Krzeptowski, Patryk Chudy, Grzegorz Sokołowski, Monika Żukowska, Anna Kusienicka, Agnieszka Seretny, Agata Kalita, Alicja Czmoczek, Jakub Gubała, Sonia Baran, Damian Klóska, Mateusz Jeż, Jacek Stępniewski, Krzysztof Szade, Agata Szade, Anna Grochot-Przęczek, Alicja Józkowicz, Witold N. Nowak

“…We found that the accumulation of G-quadruplexes is affected by heme oxygenase-1 (<i>Hmox1</i>) expression, but in a cell-type-specific manner: hematopoietic stem cells (HSCs) from <i>Hmox1^−/−</i> mice have upregulated expressions of G4-unwinding helicases (e.g., <i>Brip1</i>, <i>Pif1</i>) and show weaker staining for G-quadruplexes, whereas <i>Hmox1</i>-deficient murine induced pluripotent stem cells (iPSCs), despite the upregulation of helicases, have more G-quadruplexes, especially after exposure to exogenous heme. …”
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Supplemental Ferulic Acid Inhibits Total Body Irradiation-Mediated Bone Marrow Damage, Bone Mass Loss, Stem Cell Senescence, and Hematopoietic Defect in Mice by Enhancing Antioxida... by Sajeev Wagle, Hyun-Jaung Sim, Govinda Bhattarai, Ki-Choon Choi, Sung-Ho Kook, Jeong-Chae Lee, Young-Mi Jeon

“…While total body irradiation (TBI) is an everlasting curative therapy, the irradiation can cause long-term bone marrow (BM) injuries, along with senescence of hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) via reactive oxygen species (ROS)-induced oxidative damages. …”
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Limitations of mouse models for sickle cell disease conferred by their human globin transgene configurations by Kaitly J. Woodard, Phillip A. Doerfler, Kalin D. Mayberry, Akshay Sharma, Rachel Levine, Jonathan Yen, Virginia Valentine, Lance E. Palmer, Marc Valentine, Mitchell J. Weiss

“…Cas9 disruption of the BCL11A repressor binding motif in the γ-globin gene (HBG1 and HBG2; HBG) promoters of Berkeley mouse hematopoietic stem cells (HSCs) caused extensive death from multiple double-strand DNA breaks. …”
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Extracellular Vesicles Derived from Bone Marrow in an Early Stage of Ionizing Radiation Damage Are Able to Induce Bystander Responses in the Bone Marrow by Dávid Kis, Ilona Barbara Csordás, Eszter Persa, Bálint Jezsó, Rita Hargitai, Tünde Szatmári, Nikolett Sándor, Enikő Kis, Katalin Balázs, Géza Sáfrány, Katalin Lumniczky

“…The acute effects of EVs on the hematopoietic stem and progenitor cell pools were similar to direct irradiation effects and persisted for up to 3 months, with the hematopoietic stem cells showing the strongest bystander responses. …”
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Inter-Strain Differences in LINE-1 DNA Methylation in the Mouse Hematopoietic System in Response to Exposure to Ionizing Radiation by Isabelle R. Miousse, Jianhui Chang, Lijian Shao, Rupak Pathak, Étienne Nzabarushimana, Kristy R. Kutanzi, Reid D. Landes, Alan J. Tackett, Martin Hauer-Jensen, Daohong Zhou, Igor Koturbash

“…In this study, we investigated the endogenous DNA methylation status of the 5′ untranslated region (UTR) of LINE-1 elements in the bone marrow hematopoietic stem cells (HSCs), hematopoietic progenitor cells (HPCs), and mononuclear cells (MNCs) in radioresistant C57BL/6J and radiosensitive CBA/J mice and in response to ionizing radiation (IR). …”
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Leukemia-Induced Cellular Senescence and Stemness Alterations in Mesenchymal Stem Cells Are Reversible upon Withdrawal of B-Cell Acute Lymphoblastic Leukemia Cells by Natalia-Del Pilar Vanegas, Paola Fernanda Ruiz-Aparicio, Gloria Inés Uribe, Adriana Linares-Ballesteros, Jean-Paul Vernot

“…Mesenchymal stem cells (MSC), a key component of this BM niche, are affected in several ways with unfavorable consequences on hematopoietic stem cells favoring leukemic cells. These alterations in MSC during B-cell acute lymphoblastic leukemia (B-ALL) have not been fully studied. …”
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ENOX2 NADH Oxidase: A BCR-ABL1-Dependent Cell Surface and Secreted Redox Protein in Chronic Myeloid Leukemia by Seda Baykal, Maud Voldoire, Christophe Desterke, Nathalie Sorel, Emilie Cayssials, Hyacinthe Johnson-Ansah, Agnes Guerci-Bresler, Annelise Bennaceur-Griscelli, Jean-Claude Chome, Ali G. Turhan

“…Objective: Chronic myeloid leukemia (CML) is a disease caused by the acquisition of BCR-ABL1 fusion in hematopoietic stem cells. In this study, we focus on the oncofetal ENOX2 protein as a potential secretable biomarker in CML. …”
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A humanized orthotopic mouse model for preclinical evaluation of immunotherapy in Ewing sarcoma by Wen Luo, Wen Luo, Hai Hoang, Yanling Liao, Jian Pan, Janet Ayello, Mitchell S. Cairo, Mitchell S. Cairo, Mitchell S. Cairo, Mitchell S. Cairo

“…Here, we describe a protocol to develop an orthotopic humanized mouse model for a bone and soft tissue sarcoma, Ewing sarcoma, by transplanting fresh human cord blood CD34+ hematopoietic stem cells into young NSG-SGM3 mice combined with subsequent Ewing sarcoma patient derived cell engraftment in the tibia of the humanized mice. …”
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