Functional orthologs of honeybee CYP6AQ1 in stingless bees degrade the butenolide insecticide flupyradifurone by Xingzhi Xiao, Julian Haas, Ralf Nauen

“…Heterologous expression in High Five insect cells of these functional orthologs revealed a common coumarin substrate profile and a preference for the O-debenzylation of bulkier substrates. …”
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Projection Pursuit Multivariate Sampling of Parameter Uncertainty by Oktay Erten, Fábio P. L. Pereira, Clayton V. Deutsch

“…The superiority of the projection pursuit multivariate transform, as a sampling technique, is demonstrated in two synthetic case studies, where the random variables are considered to be uncorrelated and correlated in low (bivariate) and high (five-variate) dimensional sampling spaces. Five sampling techniques including Monte Carlo simulation, classic Latin hypercube sampling, maximin Latin hypercube sampling, Latin hypercube sampling with multidimensional uniformity, and projection pursuit multivariate transform are employed in the simulation studies, considering cases where the sample sizes <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>(</mo><mi>n</mi><mo>)</mo></mrow></semantics></math></inline-formula> are small (i.e., <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>10</mn><mo>≤</mo><mi>n</mi><mo>≤</mo><mn>100</mn></mrow></semantics></math></inline-formula>), medium (i.e., <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>100</mn><mo><</mo><mi>n</mi><mo>≤</mo><mn>1000</mn></mrow></semantics></math></inline-formula>), and large (i.e., 1000 < <i>n</i>≤ 10,000). …”
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FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells by Jesse A. Coker, Jesse A. Coker, Vittorio L. Katis, Michael Fairhead, Anja Schwenzer, Stine B. Clemmensen, Bent U. Frandsen, Willem A. de Jongh, Opher Gileadi, Nicola A. Burgess-Brown, Brian D. Marsden, Brian D. Marsden, Kim S. Midwood, Wyatt W. Yue

“…However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the Drosophila melanogaster “S2” cell line lags behind more common insect cell lines such as Sf9 or High-Five™. Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represents a bottleneck, particularly for biochemical and biophysical laboratories. …”
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Comparative Transcriptome Analysis Reveals That WSSV IE1 Protein Plays a Crucial Role in DNA Replication Control by Yixi Chen, Gaochun Wu, Chuanqi Wang, Huimin Zhang, Jinghua Zhu, Yueling Zhang, Zhongyang Lin, Defu Yao

“…Finally, we demonstrated that overexpression of IE1 promoted the expression of MCM complex genes as well as cellular DNA synthesis in insect High-Five cells. Collectively, our current data suggest that the WSSV IE1 protein is a viral effector that modulates the host DNA replication machinery for viral replication.…”
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Diagnostic accuracy of tests to detect hepatitis B surface antigen: a systematic review of the literature and meta-analysis by Amini, A, Varsaneux, O, Kelly, H, Tang, W, Chen, W, Boeras, D, Falconer, J, Tucker, J, Chou, R, Ishizaki, A, Easterbrook, P, Peeling, R

“…Pooled sensitivity of RDTs in 5 studies of HIV-positive persons was lower at 72.3% (95% CI: 67.9, 76.4) compared to that in HIV-negative persons, but specificity remained high. Five studies evaluated 8 EIAs against a chemiluminescence immunoassay reference standard with a pooled sensitivity and specificity of 88.9% (95% CI: 87.0, 90.6) and 98.4% (95% CI: 97.8, 98.8), respectively. …”

Chloride intracellular channel gene knockdown induces insect cell lines death and level increases of intracellular calcium ions by Jiqiang Song, Yanping Yu, Zhichao Yan, Shan Xiao, Xianxin Zhao, Fang Wang, Qi Fang, Gongyin Ye

“…However, the physiological function of CLIC in insects is still rarely uncovered. The ovary-derived High Five (Hi-5) cell line isolated from the cabbage looper (Trichoplusia ni) is widely used in laboratories. …”
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The relationship between abnormalities of cognitive function and cerebral activation in amyotrophic lateral sclerosis. A neuropsychological and positron emission tomography study. by Kew, J, Goldstein, L, Leigh, P, Abrahams, S, Cosgrave, N, Passingham, R, Frackowiak, R, Brooks, D

“…On the basis of their verbal fluency scores, two subgroups of the ALS patients (five high, five low scores) and a different group of five age-matched controls then underwent positron emission tomographic (PET) measurement of regional cerebral blood flow (rCBF). …”

A novel asexual blood-stage malaria vaccine candidate: PfRipr5 formulated with human-use adjuvants induces potent growth inhibitory antibodies by Eizo Takashima, Hikaru Nagaoka, Ricardo Correia, Ricardo Correia, Paula M. Alves, Paula M. Alves, António Roldão, António Roldão, Dennis Christensen, Jeffrey A. Guderian, Akihisa Fukushima, Nicola K. Viebig, Hilde Depraetere, Takafumi Tsuboi

“…In this study, PfRipr5 antigen was efficiently produced in a tank bioreactor using insect High Five cells and the baculovirus expression vector system; purified PfRipr5 was thermally stable in its monomeric form, had high purity and binding capacity to functional monoclonal anti-PfRipr antibody. …”
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Dissecting insect cell heterogeneity during influenza VLP production using single-cell transcriptomics by Marco Silvano, Marco Silvano, Nikolaus Virgolini, Nikolaus Virgolini, Ricardo Correia, Ricardo Correia, Colin Clarke, Colin Clarke, Inês A. Isidro, Inês A. Isidro, Paula M. Alves, Paula M. Alves, António Roldão, António Roldão

“…This study aimed at dissecting insect cell population heterogeneity during production of influenza VLPs in IC-BEVS using single-cell RNA-seq (scRNA-seq). High Five cell population was shown to be heterogeneous even before infection, with cell cycle being one of the factors contributing for this variation. …”
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Resting-State EEG Signal for Major Depressive Disorder Detection: A Systematic Validation on a Large and Diverse Dataset by Chien-Te Wu, Hao-Chuan Huang, Shiuan Huang, I-Ming Chen, Shih-Cheng Liao, Chih-Ken Chen, Chemin Lin, Shwu-Hua Lee, Mu-Hong Chen, Chia-Fen Tsai, Chang-Hsin Weng, Li-Wei Ko, Tzyy-Ping Jung, Yi-Hung Liu

“…The results show that (1) coherence features account for 98% of the optimal feature subset; (2) the CK-SVM outperforms other classifiers such as K-nearest neighbors (K-NN), linear discriminant analysis (LDA), and SVM; (3) the combination of the optimal feature subset and CK-SVM achieves a high five-fold cross-validation accuracy of 91.07% on the training set (140 MDD and 140 HC) and 84.16% on the independent test set (60 MDD and 60 HC). …”
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Virus-like particle vaccines with epitopes from porcine epidemic virus and transmissible gastroenteritis virus incorporated into self-assembling ADDomer platform provide clinical i... by Pengfei Du, Quanhui Yan, Xiao-Ai Zhang, Weijun Zeng, Kaiyuan Xie, Zhongmao Yuan, Xiaodi Liu, Xueyi Liu, Lihong Zhang, Keke Wu, Xiaowen Li, Shuangqi Fan, Mingqiu Zhao, Jinding Chen

“…By meticulously optimizing conditions in High Five cells, we successfully expressed and purified the recombinant proteins. …”
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Atrial Pacing Negatively Affects Left Atrial Morphological and Functional Parameters Similarly to Atrioventricular Dyssynchrony by Mindaugas Viezelis, Gintare Neverauskaite-Piliponiene, Agne Marcinkeviciene, Tomas Kazakevicius, Vytautas Zabiela, Vilius Kviesulaitis, Renaldas Jurkevicius, Aras Puodziukynas

“…Three subsets based on pacing burden were analyzed: (1) high atrial (A) low ventricular (V); (2) high A, high V and (3) low A, high V. LA function was assessed from volumetric parameters and measured strains from echocardiography. …”
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Metabolic and Molecular Response to High-Fat Diet Differs between Rats with Constitutionally High and Low Serotonin Tone by Petra Baković, Maja Kesić, Darko Kolarić, Jasminka Štefulj, Lipa Čičin-Šain

“…Consistent with previous findings, high-5HT animals fed a standard diet had poorer metabolic health. …”
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Seismic velocity structure along the Sea of Japan with large events derived from seismic tomography for whole Japanese Islands including reflection survey data and NIED MOWLAS Hi-n... by Makoto Matsubara, Tatsuya Ishiyama, Tetsuo No, Kenji Uehira, Masashi Mochizuki, Toshihiko Kanazawa, Narumi Takahashi, Shin’ichiro Kamiya

“…The west side of the hypocenter of the 2019 offshore Yamagata earthquake at depths of 10–30 km has high-V corresponding to the Mogami Trough. This high-V zone passes through Awa Island and reaches Sado Basin between Sado Island and Honshu. …”
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Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis by Maja Kesić, Petra Baković, Ranko Stojković, Jasminka Štefulj, Lipa Čičin-Šain

“…Surprisingly, adiponectin levels were increased in plasma but reduced in the WAT of high-5HT rats. A limited number of metabolic genes belonging to different functional classes showed differential expression in WAT of high-5HT compared to low-5HT rats. …”
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Role of 5-methylcytosine in determining the prognosis, tumor microenvironment, and applicability of precision medicine in patients with hepatocellular carcinoma by Mingyuan Luan, Min Zhao, Haiying Wang, Rongjian Xu, Jinzhen Cai

“…Finally, we examined the sensitivity of patients with high 5-methylcytosine score to drugs.Results: We identified two hepatocellular carcinoma-specific, 5-methylcytosine-based subtypes (clusters 1 and 2). …”
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Prognostic value of epigenetic markers for canine mast cell cancer. by Shahzar Syed, Britta J Knight, Geoffrey A Wood, Brenda Lynn Coomber

“…High 5MC levels showed significance for shorter disease-free interval (DFI) in subcutaneous cases and high 5MC levels showed poorer DFI and overall survival (OS) in cases with Kiupel's grading system high grade. …”
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Prognostic value of epigenetic markers for canine mast cell cancer by Shahzar Syed, Britta J. Knight, Geoffrey A. Wood, Brenda Lynn Coomber

“…High 5MC levels showed significance for shorter disease-free interval (DFI) in subcutaneous cases and high 5MC levels showed poorer DFI and overall survival (OS) in cases with Kiupel’s grading system high grade. …”
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Dual Threshold Voltage Integrated Organic Technology for Ultralow-power Circuits by Sodini, Charles G., Nausieda, Ivan A., Ryu, Kevin K., He, David Da, Akinwande, Akintunde Ibitayo, Bulovic, Vladimir

“…The availability of a high V [subscript T] device enables area-efficient zero-VGS current source loads. …”
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Seismogenic environment and mechanism of the Yangbi MS6.4 earthquake in Yunnan, ChinaKey points by Mengqiao Duan, Kezhen Zuo, Cuiping Zhao, Lianqing Zhou

“…High-vP/vS anomalies in its NW direction indicate cracks development and the existence of fluids or partial melts, which are unfavorable for stress accumulation and triggering large earthquakes. …”
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