A novel computational method identifies intra- and inter-species recombination events in Staphylococcus aureus and Streptococcus pneumoniae. by Lisa Sanguinetti, Simona Toti, Valerio Reguzzi, Fabio Bagnoli, Claudio Donati

“…Comparative sequence analysis frequently reveals evidences of homologous recombination occurring with different mechanisms and rates in different species, but the large-scale use of computational methods to identify recombination events is hampered by their high computational costs. …”
Get full text

Targeted recombination of homologous chromosomes using CRISPR‐Cas9 by Wonseok Son, Ki Wha Chung

“…Taken together, we suggest that targeted homologous recombination by CRISPR targeting may have specific genetic applications, such as generation of allelic combinations or chromosomal variations.…”
Get full text

The antitumorigenic roles of BRCA1–BARD1 in DNA repair and replication by Tarsounas, M, Sung, P

“…The tumour suppressor breast cancer type 1 susceptibility protein (BRCA1) promotes DNA double-strand break (DSB) repair by homologous recombination and protects DNA replication forks from attrition. …”

How G4 ligands enhance the innate immune response in BRCA1/2- deficient cells and tumours by De Visser, Y, De Visser, Y

“…The stabilisation of G-quadruplexes causes replication fork stalling resulting in DNA damage accumulation and subsequent activation of DNA damage repair pathways such as homologous recombination (Rodriguez et al., 2012). However, tumours lacking BRCA1 and BRCA2 expression, and consequently abrogation of homologous recombination, will accumulate this damage and will therefore be killed more efficiently. …”

Synthetic lethality between androgen receptor signalling and the PARP pathway in prostate cancer. by Asim, M, Tarish, F, Zecchini, HI, Sanjiv, K, Gelali, E, Massie, CE, Baridi, A, Warren, AY, Zhao, W, Ogris, C, McDuffus, L-A, Mascalchi, P, Shaw, G, Dev, H, Wadhwa, K, Wijnhoven, P, Forment, JV, Lyons, SR, Lynch, AG, O'Neill, C, Zecchini, VR, Rennie, PS, Baniahmad, A, Tavaré, S, Mills, IG, Galanty, Y, Crosetto, N, Schultz, N, Neal, D, Helleday, T

“…Emerging data demonstrate homologous recombination (HR) defects in castration-resistant prostate cancers, rendering these tumours sensitive to PARP inhibition. …”

Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase. by Kearsey, S, Stevenson, A, Toda, T, Wang, S

“…Here, we show that rad3 rqh1 mutants are normally committed to a lethal pathway of DNA repair requiring homologous recombination, but blocking this pathway by Rhp51 inactivation restores viability. …”

The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin by Krastev, DB, Li, S, Sun, Y, Wicks, AJ, Hoslett, G, Weekes, D, Badder, LM, Knight, EG, Marlow, R, Pardo, MC, Yu, L, Talele, TT, Bartek, J, Choudhary, JS, Pommier, Y, Pettitt, SJ, Tutt, ANJ, Ramadan, K, Lord, CJ

“…Poly (ADP-ribose) polymerase (PARP) inhibitors elicit antitumour activity in homologous recombination-defective cancers by trapping PARP1 in a chromatin-bound state. …”

The role of DNA polymerase eta in determining cellular responses to chemo-radiation treatment by Nicolay, N

“…These findings suggest that homologous recombination mediates S phase-dependent radioresistance associated with pol η-deficiency.…”

Molecular biology : principles of genome function / by Craig, Nancy Lynn, 1952-

Optimization of designing multiple genes encoding the same protein based on NSGA-II for efficient execution on GPUs by Donghyeon Kim, Jinsung Kim

“…In order to augment expression levels, we need to focus on both homologous recombination and codon adaptation, which are estimated by three objective functions, namely HD (Hamming distance), LRCS (length of repeated or common substring) and CAI (codon adaptation index). …”
Get full text

A Proximity Ligation Method to Detect Proteins Bound to Single-Stranded DNA after DNA End Resection at DNA Double-Strand Breaks by Faith C. Fowler, Jessica K. Tyler

“…After a DNA double-strand break, cells utilize either non-homologous end joining or homologous recombination to repair the broken DNA ends. Homologous recombination requires extensive nucleolytic processing of one of the DNA strands, resulting in long stretches of 3′ single-strand DNA overhangs. …”
Get full text

Evidence for a Xer/dif system for chromosome resolution in archaea. by Diego Cortez, Sophie Quevillon-Cheruel, Simonetta Gribaldo, Nicole Desnoues, Guennadi Sezonov, Patrick Forterre, Marie-Claude Serre

“…Homologous recombination events between circular chromosomes, occurring during or after replication, can generate dimers that need to be converted to monomers prior to their segregation at cell division. …”
Get full text

Use of an on/off tetracycline riboswitch to control protein production in Komagataella phaffii by Igor Patrick Vasconcelos Vieira, Felipe Seixas Arreguy Pimentel, Cintia Marques Coelho, Janice Lisboa De Marco, Lidia Maria Pepe de Moraes, Fernando Araripe Gonçalves Torres

“…This protein is involved in non-homologous recombination and the control of its production paves the way for the development of strains exhibiting higher rates of homologous recombination.…”
Get full text

Investigating genomic instability features in hereditary cancers by Chu, Yuen Theng

“…DNA damage causing double stranded breaks (DSBs) are efficiently repaired via non homologous end joining (NHEJ) or Homologous recombination (HR). Our focus is on mechanisms of damage response proteins, RAD51C and BRCA1 in HR, an error free repair that involves resolution of holiday junctions at sites of homologous recombination exchange of DNA. …”
Get full text

Germline mutations in PALB2, BRCA1, and RAD51C, which regulate DNA recombination repair, in patients with gastric cancer by Sahasrabudhe, R, Lott, P, Bohorquez, M, Toal, T, Estrada, A, Suarez, J, Brea-Fernández, A, Cameselle-Teijeiro, J, Pinto, C, Ramos, I, Mantilla, A, Prieto, R, Corvalan, A, Norero, E, Alvarez, C, Tapia, T, Carvallo, P, Gonzalez, L, Cock-Rada, A, Solano, A, Neffa, F, Della Valle, A, Yau, C, Soares, G, Borowsky, A, Hu, N, He, L, Han, X, Latin American Gastric Cancer Genetics Collaborative Group, Taylor, P, Goldstein, A, Torres, J, Echeverry, M, Ruiz-Ponte, C, Teixeira, M, Carvajal Carmona, L

“…Tumors that developed in patients with these mutations had a mutation signature associated with somatic homologous recombination deficiency. Our findings indicate that defects in homologous recombination increase risk for gastric cancer.…”

Targeting ATR in vivo using the novel inhibitor VE-822 results in selective sensitization of pancreatic tumors to radiation. by Fokas, E, Prevo, R, Pollard, J, Reaper, P, Charlton, P, Cornelissen, B, Vallis, K, Hammond, E, Olcina, M, Gillies McKenna, W, Muschel, R, Brunner, T

“…Chk1 phosphorylation was used to indicate ATR activity, γH2AX and 53BP1 foci as evidence of DNA damage and Rad51 foci for homologous recombination activity. Sensitivity to radiation (XRT) and gemcitabine was measured with clonogenic assays in vitro and tumor growth delay in vivo. …”

Structural maintenance of chromosomes (SMC) proteins promote homolog-independent recombination repair in meiosis crucial for germ cell genomic stability. by Jeremy S Bickel, Liting Chen, Jin Hayward, Szu Ling Yeap, Ashley E Alkers, Raymond C Chan

“…In meiosis, programmed DNA breaks repaired by homologous recombination (HR) can be processed into inter-homolog crossovers that promote the accurate segregation of chromosomes. …”
Get full text

Rdh54 stabilizes Rad51 at displacement loop intermediates to regulate genetic exchange between chromosomes. by Margaret Keymakh, Jennifer Dau, Jingyi Hu, Bryan Ferlez, Michael Lisby, J Brooks Crickard

“…Homologous recombination (HR) is a double-strand break DNA repair pathway that preserves chromosome structure. …”
Get full text

Expanding the Use of PARP Inhibitors as Monotherapy and in Combination in Triple-Negative Breast Cancer by Mariya Yordanova, Audrey Hubert, Saima Hassan

“…To better identify a patient subpopulation that will best respond to PARPi, several genomic biomarkers of homologous recombination deficiency have been tested. However, gene expression signatures associated with PARPi response can integrate different pathways in addition to homologous recombination deficiency and can be implemented in the clinic more readily. …”
Get full text

Role of Olaparib in the Management of Metastatic Castration-Resistant Prostate Cancer: A Japanese Clinician’s Perspective by Matsumoto T, Shiota M, Blas L, Eto M

“…Genomic alterations in genes involved in DNA damage repair by homologous recombination repair may predict increased sensitivity to poly-ADP ribose polymerase (PARP) inhibitors. …”
Get full text