RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p‐DNA‐PKcs by Xiaoqing Li, Shitao Zou, Liangsu Zhou, Aidi Gao, Jing Xu, Chao He, Jundong Zhou, Shuhua Wu, Yihong Chen

“…Immunofluorescence staining, comet assay, and homologous recombination (HR)/non‐homologous end‐joining (NHEJ) assays were conducted to examine the effect of RAD18 on the DNA damage response in ESCC cells. …”
Get full text

CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis by Lisa Lombardi, Sean A. Bergin, Adam Ryan, Evelyn Zuniga-Soto, Geraldine Butler

“…Here, we compared the effect of classical deletion methods (replacing target alleles with selectable markers by homologous recombination) with CRISPR-Cas9 editing in the diploid human-pathogenic yeast Candida parapsilosis. …”
Get full text

An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum by Lee, Marcus CS, Fidock, David A, Wagner, Jeffrey Charles, Goldfless, Stephen Jacob, Maddur Ganesan, Suresh, Niles, Jacquin

“…Results: Yeast homologous recombination and Gibson assembly were found to be effective strategies for successfully constructing P. falciparum plasmid vectors. …”
Get full text
Get full text
Get full text

The Ultimate (Mis)match: When DNA Meets RNA by Benoit Palancade, Rodney Rothstein

“…We summarize how in vivo and in vitro experiments support the contribution of DNA polymerases and homologous recombination proteins in the formation of RNA-containing structures, and we discuss the role of DNA repair enzymes in their removal. …”
Get full text

DNA Double-Strand Break Repairs and Their Application in Plant DNA Integration by Hexi Shen, Zhao Li

“…Cells employ two major pathways to repair DSBs: homologous recombination (HR) and non-homologous end-joining (NHEJ). …”
Get full text

Polymerase θ inhibition steps on the cGAS pedal by Chelsea M. Smith, Gaorav P. Gupta

“…Deficiencies in homologous recombination (HR) repair lead to an accumulation of DNA damage and can predispose individuals to cancer. …”
Get full text

Effects of fluorescent tags and activity status on the membrane localization of ROP GTPases by Jingtong Ruan, Zihan Yin, Peishan Yi

“…In this study, we introduce a fluorescent tag at the endogenous amino terminus of ROP4 in wild-type and rop1,2,3 triple mutant via homologous recombination and demonstrate that the fluorescent tag severely impairs ROP4 function and inhibits its localization on the plasma membrane. …”
Get full text

Primary high-grade serous cancer arising from uterosacral ligament endometriosis: two case reports by Ming Chen, Yuedi Zhang, Manman Xu, Duo Liu, Yang Yang, Shuzhong Yao

“…Genomic analysis showed no known/suspected pathogenic variations or somatic homologous recombination deficiency in the two cases. In conclusion, these rare cases of HGSC from endometriosis might indicate a new origin of ovarian type II carcinoma. …”
Get full text

Towards Plant Synthetic Genomics by Yuling Jiao, Ying Wang

“…In this perspective, we propose applying bottom-up genome synthesis in multicellular plants, starting from the model moss Physcomitrium patens, in which homologous recombination, DNA delivery, and regeneration are possible, although further optimizations are necessary. …”
Get full text

Alternative lengthening of telomeres: from molecular mechanisms to therapeutic outlooks by Jia-Min Zhang, Lee Zou

“…Most of cancers rely on telomerase to extend and maintain telomeres, but 4–11% of cancers use a homologous recombination-based pathway called alternative lengthening of telomeres (ALT). …”
Get full text

Genomic disorders: molecular mechanisms for rearrangements and conveyed phenotypes. by James R Lupski, Pawel Stankiewicz

“…The analyses of chromosome breakpoints in the proximal short arm of Chromosome 17 (17p) reveal nonallelic homologous recombination (NAHR) as a major mechanism for recurrent rearrangements whereas nonhomologous end-joining (NHEJ) can be responsible for many of the nonrecurrent rearrangements. …”
Get full text

Stitching up broken DNA ends by FANCA by Anna Palovcak, Wenjun Liu, Fenghua Yuan, Yanbin Zhang

“…Abbreviations: DSBs: DNA double-strand breaks; ICL: interstrand crosslink; ssDNA: single-stranded DNA; HR: homologous recombination; SSA: single-strand annealing; MMEJ: microhomology-mediated end joining; NHEJ: non-homologous end joining; GFP: green fluorescence protein…”
Get full text

Mechanisms of High-Grade Serous Carcinogenesis in the Fallopian Tube and Ovary: Current Hypotheses, Etiologic Factors, and Molecular Alterations by Isao Otsuka

“…Key driving events other than <i>TP53</i> mutations include homologous recombination (HR) deficiency, such as BRCA 1/2 dysfunction, and activation of the CCNE1 pathway. …”
Get full text

Redundant mechanisms for regulation of midline crossing in Drosophila. by Giorgio F Gilestro

“…Using gene targeting by homologous recombination I created new conditional alleles of robo that are sorting defective (robo(SD)). …”
Get full text

Research progress of DNA damage repair and its effect on perioperative cognitive dysfunction by RONG Yu-lan, SHI Cheng-mei, GUO Xiang-yang

“…The dynamic balance between DNA double-strand break (DSB) accumulation and repair is one of the important factors to maintain genomic stability. The homologous recombination (HR) and the non-homologous ending joining (NHEJ) are the two major pathways to repair DSB.In mature neurons of the central nervous system, the NHEJ is the main repair pathway of DSB.Recent studies in the molecular mechanism of NHEJ and peri-operative cognitive dysfunction (POCD) revealed several molecular mechanisms underlying the DNA damage repair and its links to POCD, which may provide a new therapeutic target.…”
Get full text

The bdr Gene Families of the Lyme Disease and Relapsing Fever Spirochetes: Potential Influence on Biology, Pathogenesis, and Evolution by David M. Roberts, Jason A Carlyon, Michael Theisen, Richard T. Marconi

“…Homologous alleles on different plasmids could influence the organization and evolution of the Borrelia genome by serving as foci for interplasmid homologous recombination. The plasmid-carried Borrelia direct repeat (bdr) gene family encodes polymorphic, acidic proteins with putative phosphorylation sites and transmembrane domains. …”
Get full text

Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo. by Possoz, C, Filipe, SR, Grainge, I, Sherratt, D

“…Controlled release of repressor binding led to rapid restart of the blocked replication fork without the participation of homologous recombination. Cytological tracking of fork stalling and restart showed that the replisome-associated SSB protein remains associated with the blocked fork for extended periods and that duplication of the fluorescent foci associated with the blocked operator array occurs immediately after restart, thereby demonstrating a lack of sister cohesion in the region of the array. …”

Deletion of the mouse alpha-globin regulatory element (HS -26) has an unexpectedly mild phenotype. by Anguita, E, Sharpe, J, Sloane-Stanley, J, Tufarelli, C, Higgs, D, Wood, W

“…We knocked out mHS -26 by homologous recombination and observed the surprising result that instead of the expected severe alpha-thalassemia phenotype, the mice had a mild disease. …”

Holliday junction resolution by Whitby, M

“…The Holliday junction (HJ) is a key intermediate in homologous recombination. It can be formed both as a consequence of RecA/ Rad51-catalysed strand invasion reactions, and by the reversal of impeded replication forks. …”

The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number. by Wickstead, B, Ersfeld, K, Gull, K

“…An independence of targeting frequency and target copy number is characteristic of mammalian homologous recombination and is unlike the situation in budding yeast. …”