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901
RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p‐DNA‐PKcs
Published 2022-10-01“…Immunofluorescence staining, comet assay, and homologous recombination (HR)/non‐homologous end‐joining (NHEJ) assays were conducted to examine the effect of RAD18 on the DNA damage response in ESCC cells. …”
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902
CRISPR-Cas9 Editing Induces Loss of Heterozygosity in the Pathogenic Yeast Candida parapsilosis
Published 2022-12-01“…Here, we compared the effect of classical deletion methods (replacing target alleles with selectable markers by homologous recombination) with CRISPR-Cas9 editing in the diploid human-pathogenic yeast Candida parapsilosis. …”
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903
An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum
Published 2014“…Results: Yeast homologous recombination and Gibson assembly were found to be effective strategies for successfully constructing P. falciparum plasmid vectors. …”
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904
The Ultimate (Mis)match: When DNA Meets RNA
Published 2021-06-01“…We summarize how in vivo and in vitro experiments support the contribution of DNA polymerases and homologous recombination proteins in the formation of RNA-containing structures, and we discuss the role of DNA repair enzymes in their removal. …”
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905
DNA Double-Strand Break Repairs and Their Application in Plant DNA Integration
Published 2022-02-01“…Cells employ two major pathways to repair DSBs: homologous recombination (HR) and non-homologous end-joining (NHEJ). …”
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906
Polymerase θ inhibition steps on the cGAS pedal
Published 2023-06-01“…Deficiencies in homologous recombination (HR) repair lead to an accumulation of DNA damage and can predispose individuals to cancer. …”
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907
Effects of fluorescent tags and activity status on the membrane localization of ROP GTPases
Published 2024-12-01“…In this study, we introduce a fluorescent tag at the endogenous amino terminus of ROP4 in wild-type and rop1,2,3 triple mutant via homologous recombination and demonstrate that the fluorescent tag severely impairs ROP4 function and inhibits its localization on the plasma membrane. …”
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908
Primary high-grade serous cancer arising from uterosacral ligament endometriosis: two case reports
Published 2022-07-01“…Genomic analysis showed no known/suspected pathogenic variations or somatic homologous recombination deficiency in the two cases. In conclusion, these rare cases of HGSC from endometriosis might indicate a new origin of ovarian type II carcinoma. …”
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909
Towards Plant Synthetic Genomics
Published 2023-01-01“…In this perspective, we propose applying bottom-up genome synthesis in multicellular plants, starting from the model moss Physcomitrium patens, in which homologous recombination, DNA delivery, and regeneration are possible, although further optimizations are necessary. …”
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910
Alternative lengthening of telomeres: from molecular mechanisms to therapeutic outlooks
Published 2020-03-01“…Most of cancers rely on telomerase to extend and maintain telomeres, but 4–11% of cancers use a homologous recombination-based pathway called alternative lengthening of telomeres (ALT). …”
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911
Genomic disorders: molecular mechanisms for rearrangements and conveyed phenotypes.
Published 2005-12-01“…The analyses of chromosome breakpoints in the proximal short arm of Chromosome 17 (17p) reveal nonallelic homologous recombination (NAHR) as a major mechanism for recurrent rearrangements whereas nonhomologous end-joining (NHEJ) can be responsible for many of the nonrecurrent rearrangements. …”
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912
Stitching up broken DNA ends by FANCA
Published 2018-11-01“…Abbreviations: DSBs: DNA double-strand breaks; ICL: interstrand crosslink; ssDNA: single-stranded DNA; HR: homologous recombination; SSA: single-strand annealing; MMEJ: microhomology-mediated end joining; NHEJ: non-homologous end joining; GFP: green fluorescence protein…”
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913
Mechanisms of High-Grade Serous Carcinogenesis in the Fallopian Tube and Ovary: Current Hypotheses, Etiologic Factors, and Molecular Alterations
Published 2021-04-01“…Key driving events other than <i>TP53</i> mutations include homologous recombination (HR) deficiency, such as BRCA 1/2 dysfunction, and activation of the CCNE1 pathway. …”
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914
Redundant mechanisms for regulation of midline crossing in Drosophila.
Published 2008-01-01“…Using gene targeting by homologous recombination I created new conditional alleles of robo that are sorting defective (robo(SD)). …”
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915
Research progress of DNA damage repair and its effect on perioperative cognitive dysfunction
Published 2022-04-01“…The dynamic balance between DNA double-strand break (DSB) accumulation and repair is one of the important factors to maintain genomic stability. The homologous recombination (HR) and the non-homologous ending joining (NHEJ) are the two major pathways to repair DSB.In mature neurons of the central nervous system, the NHEJ is the main repair pathway of DSB.Recent studies in the molecular mechanism of NHEJ and peri-operative cognitive dysfunction (POCD) revealed several molecular mechanisms underlying the DNA damage repair and its links to POCD, which may provide a new therapeutic target.…”
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916
The bdr Gene Families of the Lyme Disease and Relapsing Fever Spirochetes: Potential Influence on Biology, Pathogenesis, and Evolution
Published 2000-04-01“…Homologous alleles on different plasmids could influence the organization and evolution of the Borrelia genome by serving as foci for interplasmid homologous recombination. The plasmid-carried Borrelia direct repeat (bdr) gene family encodes polymorphic, acidic proteins with putative phosphorylation sites and transmembrane domains. …”
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917
Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo.
Published 2006“…Controlled release of repressor binding led to rapid restart of the blocked replication fork without the participation of homologous recombination. Cytological tracking of fork stalling and restart showed that the replisome-associated SSB protein remains associated with the blocked fork for extended periods and that duplication of the fluorescent foci associated with the blocked operator array occurs immediately after restart, thereby demonstrating a lack of sister cohesion in the region of the array. …”
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918
Deletion of the mouse alpha-globin regulatory element (HS -26) has an unexpectedly mild phenotype.
Published 2002“…We knocked out mHS -26 by homologous recombination and observed the surprising result that instead of the expected severe alpha-thalassemia phenotype, the mice had a mild disease. …”
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919
Holliday junction resolution
Published 2007“…The Holliday junction (HJ) is a key intermediate in homologous recombination. It can be formed both as a consequence of RecA/ Rad51-catalysed strand invasion reactions, and by the reversal of impeded replication forks. …”
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920
The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number.
Published 2003“…An independence of targeting frequency and target copy number is characteristic of mammalian homologous recombination and is unlike the situation in budding yeast. …”
Journal article