First-line treatment of advanced ovarian cancer: an expert update by Branka Petrić-Miše

“…After achieving the response to first-line chemotherapy, maintenance therapy with poly-adenosine-diphosphate-ribose-polymerase (PARP) inhibitors prolongs PFS in patients with homologous recombination deficiency (HRD). Patients with BRCA mutations obtain the most significant benefit.…”
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New Discoveries on Protein Recruitment and Regulation during the Early Stages of the DNA Damage Response Pathways by Kelly L. Waters, Donald E. Spratt

“…The five major pathways involved in repairing eukaryotic DNA include base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), non-homologous end joining (NHEJ), and homologous recombination (HR). When these pathways do not properly repair damaged DNA, genomic stability is compromised and can contribute to diseases such as cancer. …”
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Polymorphisms of the DNA repair genes XRCC1 and XRCC3 in a Brazilian population by Márcia Cristina Duarte, Jucimara Colombo, Andréa Regina Baptista Rossit, Ana Elizabete Silva

“…We genotyped 300 healthy Southeastern Brazilian individuals (262 of European ancestry and 38 of African ancestry) for polymorphisms of codons 194 and 399 of the XRCC1 base excision repair pathway gene and of codon 241 of the XRCC3 homologous recombination repair pathway gene. The allele frequencies were 0.07 for the Arg194Trp and 0.33 for the Arg399Gln codons of the XRCC1 gene and 0.35 for the Thr241Met codon of the XRCC3 gene. …”
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Optimization of a large-scale gene disruption protocol in <it>Dictyostelium </it>and analysis of conserved genes of unknown function by Escalante Ricardo, Robles Alicia, Torija Patricia

“…The selection of homologous recombination events was also performed by PCR. …”
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In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells. by Richard A Heins, Jay H Choi, Takayuki Sohka, Marc Ostermeier

“…We have previously demonstrated that the non-homologous recombination of the genes encoding maltose binding protein (MBP) and TEM1 β-lactamase (BLA) can result in fusion proteins in which β-lactamase enzyme activity is allosterically regulated by maltose. …”
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The Chromatin Landscape Channels DNA Double-Strand Breaks to Distinct Repair Pathways by Zulong Chen, Jessica K. Tyler

“…DNA double-strand breaks (DSBs), the most deleterious DNA lesions, are primarily repaired by two pathways, namely homologous recombination (HR) and non-homologous end joining (NHEJ), the choice of which is largely dependent on cell cycle phase and the local chromatin landscape. …”
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Beta HPV Deregulates Double-Strand Break Repair by Changkun Hu, Nicholas Wallace

“…Of the two, beta HPV E6 appears to have the greatest ability to increase UV mutagenesis, by attenuating two major double-strand break (DSB) repair pathways, homologous recombination, and non-homologous end-joining. Here, we review this dysregulation of DSB repair and emerging approaches that can be used to further these efforts.…”
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A fine-structure map of spontaneous mitotic crossovers in the yeast Saccharomyces cerevisiae. by Phoebe S Lee, Patricia W Greenwell, Margaret Dominska, Malgorzata Gawel, Monica Hamilton, Thomas D Petes

“…Homologous recombination is an important mechanism for the repair of DNA damage in mitotically dividing cells. …”
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The role of carboplatin in the neoadjuvant chemotherapy treatment of triple negative breast cancer by Aurelio Bartolome Castrellon, Ihor Pidhorecky, Vicente Valero, Luis Estuardo Raez

“…We discuss the homologous recombination deficiency score and the somatic or germline mutation for BRCA as potential biomarkers for future selection of patients that could benefit from the addition of Cb to NACT.…”
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Gene targeting of mouse Tardbp negatively affects Masp2 expression. by Samar Dib, Shangxi Xiao, Denise Miletic, Janice Robertson

“…Here we show that although the targeting vector was specifically designed to not overlap with Tardbp adjacent genes, the homologous recombination event affected the expression of a downstream gene, Masp2. …”
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Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria by Simon Sau Yin Law, Geoffrey Liou, Yukiko Nagai, Joan Giménez-Dejoz, Ayaka Tateishi, Kousuke Tsuchiya, Yutaka Kodama, Tsuyohiko Fujigaya, Keiji Numata

“…Here, the authors hybridize polymer-coated carbon nanotubes with functional peptides to deliver plasmid DNA cargo into intact plant mitochondria for transient expression and homologous recombination at high efficiency.…”
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A unifying model that explains the origins of human inverted copy number variants. by Bonita J Brewer, Maitreya J Dunham, M K Raghuraman

“…Different mechanisms have been proposed to account for the novel junctions in these complex architectures, including aberrant forms of DNA replication, non-allelic homologous recombination, and various pathways that repair DNA breaks. …”
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Tumorigenesis in mice with a fusion of the leukaemia oncogene Mll and the bacterial lacZ gene. by Dobson, C, Warren, A, Pannell, R, Forster, A, Rabbitts, T

“…The consequence of MLL truncation and fusion has been analysed by joining exon 8 of Mll with the bacterial lacZ gene using homologous recombination in mouse embryonic stem cells. We show that this fusion is sufficient to cause embryonic stem cell-derived acute leukaemias in chimeric mice, and these tumours occur with long latency compared with those found in MLL-Af9 chimeric mice. …”

Transformation asymmetry and the evolution of the bacterial accessory genome by Apagyi, K, Fraser, C, Croucher, N

“…Bacterial transformation can insert or delete genomic islands (GIs), depending on the donor and recipient genotypes, if an homologous recombination spans the GI's integration site and includes sufficiently long flanking homologous arms. …”

Inferring malaria parasite population structure from serological networks. by Buckee, C, Bull, P, Gupta, S

“…Knowledge of the population structure and dynamics of these genes is important for designing control programmes and understanding the acquisition of immunity to malaria; however, high rates of homologous and non-homologous recombination as well as complex patterns of expression within hosts have hindered attempts to elucidate these structures experimentally. …”

Histone deacetylase inhibitors as radiosensitisers: Effects on DNA damage signalling and repair by Groselj, B, Sharma, N, Hamdy, F, Kerr, M, Kiltie, A

“…Cells have developed mechanisms to repair such DSB through two major pathways: non-homologous end-joining and homologous recombination. Here, we explore the current evidence for the use of HDACi in combination with irradiation, focusing on the effects of HDACi on DNA damage signalling and repair in vitro. …”

Inferring malaria parasite population structure from serological networks by Buckee, C, Bull, P, Gupta, S

“…Knowledge of the population structure and dynamics of these genes is important for designing control programmes and understanding the acquisition of immunity to malaria; however, high rates of homologous and non-homologous recombination as well as complex patterns of expression within hosts have hindered attempts to elucidate these structures experimentally. …”

Yeast Sen1 helicase protects the genome from transcription-associated instability by Mischo, H, Gómez-González, B, Grzechnik, P, Rondón, A, Wei, W, Steinmetz, L, Aguilera, A, Proudfoot, N

“…SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability.…”

Structure of the helicase domain of DNA polymerase theta reveals a possible role in the microhomology-mediated end-joining pathway by Newman, J, Cooper, C, Aitkenhead, H, Gileadi, O

“…Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to inhibit cell survival in a subset of these, making it an attractive target for cancer treatment. …”

A method for the generation of YAC transgenic mice by pronuclear microinjection. by Schedl, A, Larin, Z, Montoliu, L, Thies, E, Kelsey, G, Lehrach, H, Schütz, G

“…Introduction by homologous recombination in yeast of sequences permitting YAC amplification greatly facilitated the isolation of YAC DNA at high concentrations. …”