Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration by Ramijan, K, Zhang, Z, van Wezel, GP, Claessen, D

“…Strikingly, the deletion sites were preceded by insertion sequence elements, suggesting that the rearrangements may have been caused by replicative transposition and homologous recombination between both replicons. These data indicate that protoplast formation is a stressful process that can lead to profound genetic changes.…”

Histone deacetylase inhibitors as radiosensitisers: effects on DNA damage signalling and repair. by Groselj, B, Sharma, N, Hamdy, F, Kerr, M, Kiltie, A

“…Cells have developed mechanisms to repair such DSB through two major pathways: non-homologous end-joining and homologous recombination. Here, we explore the current evidence for the use of HDACi in combination with irradiation, focusing on the effects of HDACi on DNA damage signalling and repair in vitro. …”

DNA interstrand cross-link repair in Saccharomyces cerevisiae. by Lehoczký, P, McHugh, P, Chovanec, M

“…For Escherichia coli, a pathway which combines nucleotide excision repair (NER) and homologous recombination repair (HRR) to eliminate ICL has been characterized in detail, both genetically and biochemically. …”

OCTOVA: A randomised phase II trial of olaparib, chemotherapy, or olaparib and cediranib in patients with BRCA-mutated platinumresistant ovarian cancer by Nicum, S, Strauss, V, McGregor, N, McNeish, I, Roux, R, Hall, M, Michael, A, Roberts, C

“…Angiogenesis inhibitors, such as the oral tyrosine kinase inhibitor cediranib, are active in OC, and have shown additive effects when combined with PARP inhibitors preclinically, as hypoxia-induced downregulation of homologous recombination repair genes, BRCA1, 2 and RAD51 enhances PARP inhibitor sensitivity. …”

Inference of bacterial microevolution using multilocus sequence data. by Didelot, X, Falush, D

“…We describe a model-based method for using multilocus sequence data to infer the clonal relationships of bacteria and the chromosomal position of homologous recombination events that disrupt a clonal pattern of inheritance. …”

DNA helicases Sgs1 and BLM promote DNA double-strand break resection. by Gravel, S, Chapman, R, Magill, C, Jackson, S

“…A key cellular response to DNA double-strand breaks (DSBs) is 5'-to-3' DSB resection by nucleases to generate regions of ssDNA that then trigger cell cycle checkpoint signaling and DSB repair by homologous recombination (HR). Here, we reveal that in the absence of exonuclease Exo1 activity, deletion or mutation of the Saccharomyces cerevisiae RecQ-family helicase, Sgs1, causes pronounced hypersensitivity to DSB-inducing agents. …”

Crystal structure of an octameric RuvA-Holliday junction complex. by Roe, S, Barlow, T, Brown, T, Oram, M, Keeley, A, Tsaneva, I, Pearl, L

“…Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. …”

Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. by Beumer, K, Trautman, J, Bozas, A, Liu, J, Rutter, J, Gall, J, Carroll, D

“…Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10% of all targets at a given locus. …”

ATRX dysfunction induces replication defects in primary mouse cells by Clynes, D, Jelinska, C, Xella, B, Ayyub, H, Taylor, S, Mitson, M, Bachrati, C, Higgs, D, Gibbons, R

“…Mutations in ATRX have recently been identified in tumours which maintain their telomeres by a telomerase independent pathway involving homologous recombination thought to be triggered by DNA damage. …”

Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. by Dubarry, N, Barre, F

“…This is notably the case when chromosome dimers are formed by homologous recombination. Chromosome segregation then requires the activity of a double-stranded DNA transporter anchored at the septum by an integral membrane domain, FtsK. …”

The assembly of large BACs by in vivo recombination. by Mejía, J, Larin, Z

“…The specificity of Cre recombinase for loxP sites minimizes the possibility of intramolecular rearrangements, unlike previous techniques using general homologous recombination in E. coli, and makes our method compatible with the presence of large arrays of repeated sequences in cloned DNA. …”

Yeast Sen1 helicase protects the genome from transcription-associated instability. by Mischo, H, Gómez-González, B, Grzechnik, P, Rondón, A, Wei, W, Steinmetz, L, Aguilera, A, Proudfoot, N

“…SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability.…”

CDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair. by Esashi, F, Christ, N, Gannon, J, Liu, Y, Hunt, T, Jasin, M, West, S

“…The underlying basis of tumorigenesis is thought to be linked to defects in DNA double-strand break repair by homologous recombination. Here we show that the carboxy-terminal region of BRCA2, which interacts directly with the essential recombination protein RAD51, contains a site (serine 3291; S3291) that is phosphorylated by cyclin-dependent kinases. …”

The role of the p53 protein in the apoptotic response. by Lane, D, Lu, X, Hupp, T, Hall, P

“…In mice in which the p53 gene has been inactivated by homologous recombination this response is profoundly diminished. …”

Construction of baculovirus transfer vectors for insect cell expression of human choline and ethanolamine kinase isoforms by Chang Chia, Than

“…The constructs were ready for homologous recombination with Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. …”
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Radiosensitization by Hyperthermia: The Effects of Temperature, Sequence, and Time Interval in Cervical Cell Lines by Xionge Mei, Rosemarie ten Cate, Caspar M. van Leeuwen, Hans M. Rodermond, Lidewij de Leeuw, Dionysia Dimitrakopoulou, Lukas J.A. Stalpers, Johannes Crezee, H. Petra Kok, Nicolaas A.P. Franken, Arlene L. Oei

“…</b>Radiation-induced DNA breaks can be repaired by nonhomologous end-joining (NHEJ) or homologous recombination (HR). Hyperthermia can temporarily inactivate homologous recombination. …”
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Endogenous Plasmids and Chromosomal Genome Reduction in the Cardinium Endosymbiont of Dermatophagoides farinae by Qing Xiong, Cathy Sin-Hang Fung, Xiaojun Xiao, Angel Tsz-Yau Wan, Mingqiang Wang, Pavel Klimov, Yaning Ren, Kevin Yi Yang, Jan Hubert, Yubao Cui, Xiaoyu Liu, Stephen Kwok-Wing Tsui

“…DF not only harbor massive homologous sequences that enable homologous recombination with the chromosome, but also can provide necessary functional proteins when the coding genes decayed in the chromosomal genome. …”
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Isolation and engineering of a high affinity antibody against P-selectin glycoprotein ligand-1 (PSGL-1) by Swers, Jeffrey Seth

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Mechanisms of PARP-Inhibitor-Resistance in BRCA-Mutated Breast Cancer and New Therapeutic Approaches by Sayra Dilmac, Bulent Ozpolat

“…PARP inhibitors induce “synthetic lethality” in patients with tumors with a homologous-recombination-deficiency (HRD). Several molecular mechanisms have been identified as causing PARP-inhibitor-resistance. …”
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Contemporary Systemic Therapy Intensification for Prostate Cancer: A Review for General Practitioners in Oncology by Anupam Batra, Daniel Glick, Mario Valdes

“…Novel strategies use hormonal agents, chemotherapy, homologous recombination repair inhibitors, and radioligand therapy or combination strategies in addition to androgen deprivation therapy. …”
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