DNA Polymerase θ: A Cancer Drug Target with Reverse Transcriptase Activity by Xiaojiang S. Chen, Richard T. Pomerantz

“…The emergence of precision medicine from the development of Poly (ADP-ribose) polymerase (PARP) inhibitors that preferentially kill cells defective in homologous recombination has sparked wide interest in identifying and characterizing additional DNA repair enzymes that are synthetic lethal with HR factors. …”
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Deletion of RAD52 in Saccharomyces cerevisiae severely decreases frequencies of Agrobacterium genetic transformation mediated by either an integrative or a replicating binary vecto... by Jalal Soltani, Raymond Brandt, Gerard Paul H. van Heusden, Paul J. J. Hooykaas

“…Inside the host cell, RAD52 which is involved in DNA repair is a key gene determining integration of T-DNA by homologous recombination. Here, using Saccharomyces cerevisiae haploid strains BY4741 and BY4742, a rad52 diploid deletion strain is constructed in yeast BY4743 background.This model organism was employed to show that RAD52 deletion severely decreases frequencies of Agrobacterium genetic transformation mediated by either an integrative T-DNA or a circular non-integrative T-DNA. …”
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Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium by Tetsuya Muramoto, Hoshie Iriki, Jun Watanabe, Takefumi Kawata

“…In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. …”
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Liquid biopsy in ovarian cancer in China and the world: current status and future perspectives by Hui Zhang, Lingxia Wang, Huanwen Wu

“…While serum cancer antigen 125 (CA-125) has been successfully used as a blood-borne marker and is routinely monitored in patients with OC, CA-125 testing has limitations in sensitivity and specificity and does not provide direct information on important molecular characteristics that can guide treatment decisions, such as homologous recombination repair deficiency. We comprehensively review the literature surrounding methods based on liquid biopsies, which may provide improvements in sensitivity, specificity, and provide valuable additional information to enable early diagnosis, monitoring of recurrence/progression/therapeutic response, and accurate prognostication for patients with OC, highlighting applications of this research in China.…”
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Effects of p53 and ATRX inhibition on telomeric recombination in aging fibroblasts by Ion Udroiu, Jessica Marinaccio, Antonella Sgura

“…Silencing of ATRX greatly increases telomeric recombination in young cells, but is not able to overcome senescence-induced repression of homologous recombination. Conversely, inhibition of both p53 and ATRX leads to a phenotype reminiscent of some aspects of ALT activity, with a further increase of APB, a decrease of telomere shortening (and increased proliferation) and, above all, an increase of telomeric recombination.…”
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Liquid-liquid phase separation in DNA double-strand breaks repair by Yun-Long Wang, Wan-Wen Zhao, Jie Shi, Xiang-Bo Wan, Jian Zheng, Xin-Juan Fan

“…DSBs are mainly repaired by homologous recombination (HR) and nonhomologous end joining (NHEJ). …”
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DNA Damage Response Regulation by Histone Ubiquitination by Miyu Sekiguchi, Nobuko Matsushita

“…There are two major pathways for DSB repair, viz., nonhomologous end joining and homologous recombination, and the choice of the pathway is partially controlled by posttranslational modifications of histones, including ubiquitination. …”
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Molecular Testing in Ovarian Tumours: Challenges from the Pathologist’s Perspective by Kate Dinneen, Rupali Arora

“…The use of molecular testing to direct diagnosis and treatment options in ovarian tumours has rapidly expanded in recent years, in particular with regard to the recommendation for routine homologous recombination deficiency (HRD) testing in all patients with high-grade ovarian epithelial tumours. …”
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Opinion: PARP inhibitors in cancer—what do we still need to know? by Andrew J. Wicks, Dragomir B. Krastev, Stephen J. Pettitt, Andrew N. J. Tutt, Christopher J. Lord

“…PARP inhibitors (PARPi) have been demonstrated to exhibit profound anti-tumour activity in individuals whose cancers have a defect in the homologous recombination DNA repair pathway. Here, we describe the current consensus as to how PARPi work and how drug resistance to these agents emerges. …”
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Sugar Import and Phytopathogenicity of Spiroplasma citri: Glucose and Fructose Play Distinct Roles by Aurélie André, Mickaël Maucourt, Annick Moing, Dominique Rolin, Joël Renaudin

“…A S. citri mutant was obtained by disruption of ptsG through homologous recombination and was proved unable to import glucose. …”
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Development of the Connecting Piece in ODF1-Deficient Mouse Spermatids by Sigrid Hoyer-Fender

“…The depletion of ODF1 by homologous recombination in mice led to male infertility. Although sperm tails were present in the epididymis, no intact spermatozoa were found. …”
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Production of Recombinant African Swine Fever Viruses: Speeding Up the Process by Anusyah Rathakrishnan, Katy Moffat, Ana Luisa Reis, Linda K. Dixon

“…This method combines the conventional homologous recombination with fluorescent-activated cells sorting (FACS), to isolate and purify viruses expressing fluorescent reporter genes. …”
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OsHUS1 facilitates accurate meiotic recombination in rice. by Lixiao Che, Kejian Wang, Ding Tang, Qiaoquan Liu, Xiaojun Chen, Yafei Li, Qing Hu, Yi Shen, Hengxiu Yu, Minghong Gu, Zhukuan Cheng

“…Although early homologous recombination events occurred normally, the number of interference-sensitive crossovers was reduced in the absence of OsHUS1. …”
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Prognostic and Predictive Biomarkers in Familial Breast Cancer by Siddhartha Deb, Anannya Chakrabarti, Stephen B. Fox

“…Furthermore, tumours showing homologous recombination deficiency, due to loss of <i>BRCA1</i>, <i>BRCA2</i>, <i>PALB2</i> and <i>CHEK2</i> function, have been shown to be especially sensitive to platinum-based chemotherapeutics and PARP inhibition. …”
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Understanding Resistance Mechanisms and Expanding the Therapeutic Utility of PARP Inhibitors by Joline S. J. Lim, David S. P. Tan

“…Poly-(ADP-ribose) polymerase (PARP) inhibitors act through synthetic lethality in cells with defects in homologous recombination (HR) DNA repair caused by molecular aberrations such as BRCA mutations, and is approved for treatment in ovarian cancer, with promising clinical activity against other HR defective tumors including breast and prostate cancers. …”
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PARylation of BRCA1 limits DNA break resection through BRCA2 and EXO1 by Samuele Lodovichi, Roberto Quadri, Sarah Sertic, Achille Pellicioli

“…Summary: The nucleolytic processing (resection) of a DNA double-strand break (DSB) is a critical step to repair the lesion by homologous recombination (HR). PARylation, which is the attachment of poly(ADP-ribose) (PAR) units to specific targets by PAR polymerases (PARPs), regulates many steps of HR, including resection. …”
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A genetic and structural study of genome rearrangements mediated by high copy repeat Ty1 elements. by Jason E Chan, Richard D Kolodner

“…The majority of the Ty912-mediated GCRs observed were monocentric nonreciprocal translocations mediated by RAD52-dependent homologous recombination (HR) between Ty912 and a Ty element on another chromosome arm. …”
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Molecular Mechanisms of Prostate Cancer Development in the Precision Medicine Era: A Comprehensive Review by Shigekatsu Maekawa, Ryo Takata, Wataru Obara

“…Additionally, treatments targeting homologous recombination repair mutations and the PI3K/Akt pathway have been developed and are increasingly accessible, and multiple clinical trials have investigated the efficacy of immune checkpoint inhibitors. …”
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DNA cytosine deamination is associated with recurrent Somatic Copy Number Alterations in stomach adenocarcinoma by Yilin Shi, Huangxuan Shen

“…Somatic Copy Number Alterations (SCNAs), which result in Homologous recombination (HR) deficiency in double-strand break repair, are associated with the progression of STAD. …”
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Versatile CRISPR/Cas9 Systems for Genome Editing in <i>Ustilago maydis</i> by Sarah-Maria Wege, Katharina Gejer, Fabienne Becker, Michael Bölker, Johannes Freitag, Björn Sandrock

“…These include targeted gene deletion via homologous recombination of short double-stranded oligonucleotides, introduction of point mutations, heterologous complementation at the genomic locus, and endogenous N-terminal tagging with the fluorescent protein mCherry. …”
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