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1041
DNA Polymerase θ: A Cancer Drug Target with Reverse Transcriptase Activity
Published 2021-07-01“…The emergence of precision medicine from the development of Poly (ADP-ribose) polymerase (PARP) inhibitors that preferentially kill cells defective in homologous recombination has sparked wide interest in identifying and characterizing additional DNA repair enzymes that are synthetic lethal with HR factors. …”
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1042
Deletion of RAD52 in Saccharomyces cerevisiae severely decreases frequencies of Agrobacterium genetic transformation mediated by either an integrative or a replicating binary vecto...
Published 2012-06-01“…Inside the host cell, RAD52 which is involved in DNA repair is a key gene determining integration of T-DNA by homologous recombination. Here, using Saccharomyces cerevisiae haploid strains BY4741 and BY4742, a rad52 diploid deletion strain is constructed in yeast BY4743 background.This model organism was employed to show that RAD52 deletion severely decreases frequencies of Agrobacterium genetic transformation mediated by either an integrative T-DNA or a circular non-integrative T-DNA. …”
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1043
Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium
Published 2019-01-01“…In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. …”
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1044
Liquid biopsy in ovarian cancer in China and the world: current status and future perspectives
Published 2023-12-01“…While serum cancer antigen 125 (CA-125) has been successfully used as a blood-borne marker and is routinely monitored in patients with OC, CA-125 testing has limitations in sensitivity and specificity and does not provide direct information on important molecular characteristics that can guide treatment decisions, such as homologous recombination repair deficiency. We comprehensively review the literature surrounding methods based on liquid biopsies, which may provide improvements in sensitivity, specificity, and provide valuable additional information to enable early diagnosis, monitoring of recurrence/progression/therapeutic response, and accurate prognostication for patients with OC, highlighting applications of this research in China.…”
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1045
Effects of p53 and ATRX inhibition on telomeric recombination in aging fibroblasts
Published 2024-01-01“…Silencing of ATRX greatly increases telomeric recombination in young cells, but is not able to overcome senescence-induced repression of homologous recombination. Conversely, inhibition of both p53 and ATRX leads to a phenotype reminiscent of some aspects of ALT activity, with a further increase of APB, a decrease of telomere shortening (and increased proliferation) and, above all, an increase of telomeric recombination.…”
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1046
Liquid-liquid phase separation in DNA double-strand breaks repair
Published 2023-11-01“…DSBs are mainly repaired by homologous recombination (HR) and nonhomologous end joining (NHEJ). …”
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1047
DNA Damage Response Regulation by Histone Ubiquitination
Published 2022-07-01“…There are two major pathways for DSB repair, viz., nonhomologous end joining and homologous recombination, and the choice of the pathway is partially controlled by posttranslational modifications of histones, including ubiquitination. …”
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1048
Molecular Testing in Ovarian Tumours: Challenges from the Pathologist’s Perspective
Published 2023-06-01“…The use of molecular testing to direct diagnosis and treatment options in ovarian tumours has rapidly expanded in recent years, in particular with regard to the recommendation for routine homologous recombination deficiency (HRD) testing in all patients with high-grade ovarian epithelial tumours. …”
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1049
Opinion: PARP inhibitors in cancer—what do we still need to know?
Published 2022-07-01“…PARP inhibitors (PARPi) have been demonstrated to exhibit profound anti-tumour activity in individuals whose cancers have a defect in the homologous recombination DNA repair pathway. Here, we describe the current consensus as to how PARPi work and how drug resistance to these agents emerges. …”
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1050
Sugar Import and Phytopathogenicity of Spiroplasma citri: Glucose and Fructose Play Distinct Roles
Published 2005-01-01“…A S. citri mutant was obtained by disruption of ptsG through homologous recombination and was proved unable to import glucose. …”
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1051
Development of the Connecting Piece in ODF1-Deficient Mouse Spermatids
Published 2022-09-01“…The depletion of ODF1 by homologous recombination in mice led to male infertility. Although sperm tails were present in the epididymis, no intact spermatozoa were found. …”
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1052
Production of Recombinant African Swine Fever Viruses: Speeding Up the Process
Published 2020-06-01“…This method combines the conventional homologous recombination with fluorescent-activated cells sorting (FACS), to isolate and purify viruses expressing fluorescent reporter genes. …”
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1053
OsHUS1 facilitates accurate meiotic recombination in rice.
Published 2014-06-01“…Although early homologous recombination events occurred normally, the number of interference-sensitive crossovers was reduced in the absence of OsHUS1. …”
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1054
Prognostic and Predictive Biomarkers in Familial Breast Cancer
Published 2023-02-01“…Furthermore, tumours showing homologous recombination deficiency, due to loss of <i>BRCA1</i>, <i>BRCA2</i>, <i>PALB2</i> and <i>CHEK2</i> function, have been shown to be especially sensitive to platinum-based chemotherapeutics and PARP inhibition. …”
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1055
Understanding Resistance Mechanisms and Expanding the Therapeutic Utility of PARP Inhibitors
Published 2017-08-01“…Poly-(ADP-ribose) polymerase (PARP) inhibitors act through synthetic lethality in cells with defects in homologous recombination (HR) DNA repair caused by molecular aberrations such as BRCA mutations, and is approved for treatment in ovarian cancer, with promising clinical activity against other HR defective tumors including breast and prostate cancers. …”
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1056
PARylation of BRCA1 limits DNA break resection through BRCA2 and EXO1
Published 2023-02-01“…Summary: The nucleolytic processing (resection) of a DNA double-strand break (DSB) is a critical step to repair the lesion by homologous recombination (HR). PARylation, which is the attachment of poly(ADP-ribose) (PAR) units to specific targets by PAR polymerases (PARPs), regulates many steps of HR, including resection. …”
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1057
A genetic and structural study of genome rearrangements mediated by high copy repeat Ty1 elements.
Published 2011-05-01“…The majority of the Ty912-mediated GCRs observed were monocentric nonreciprocal translocations mediated by RAD52-dependent homologous recombination (HR) between Ty912 and a Ty element on another chromosome arm. …”
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1058
Molecular Mechanisms of Prostate Cancer Development in the Precision Medicine Era: A Comprehensive Review
Published 2024-01-01“…Additionally, treatments targeting homologous recombination repair mutations and the PI3K/Akt pathway have been developed and are increasingly accessible, and multiple clinical trials have investigated the efficacy of immune checkpoint inhibitors. …”
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1059
DNA cytosine deamination is associated with recurrent Somatic Copy Number Alterations in stomach adenocarcinoma
Published 2023-10-01“…Somatic Copy Number Alterations (SCNAs), which result in Homologous recombination (HR) deficiency in double-strand break repair, are associated with the progression of STAD. …”
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1060
Versatile CRISPR/Cas9 Systems for Genome Editing in <i>Ustilago maydis</i>
Published 2021-02-01“…These include targeted gene deletion via homologous recombination of short double-stranded oligonucleotides, introduction of point mutations, heterologous complementation at the genomic locus, and endogenous N-terminal tagging with the fluorescent protein mCherry. …”
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