Targeting BRCA1 and BRCA2 deficiencies with G-quadruplex-interacting compounds by Zimmer, J, Tacconi, E, Folio, C, Badie, S, Porru, M, Klare, K, Tumiati, M, Markkanen, E, Halder, S, Ryan, A, Jackson, S, Ramadan, K, Kuznetsov, S, Biroccio, A, Sale, J, Tarsounas, M

“…Stalled replication forks can be stabilized and restarted by homologous recombination (HR), which also repairs DNA double-strand breaks (DSBs) arising at collapsed forks. …”

The CST complex mediates end protection at double-strand breaks and promotes PARP inhibitor sensitivity in BRCA1-deficient cells by Barazas, M, Annunziato, S, Pettitt, S, de Krijger, I, Ghezraoui, H, Roobol, S, Lutz, C, Frankum, J, Song, F, Brough, R, Evers, B, Gogola, E, Bhin, J, van de Ven, M, van Gent, D, Jacobs, J, Chapman, J, Lord, C, Jonkers, J, Rottenberg, S

“…This interaction is counteracted by the restoration of BRCA1-independent homologous recombination through loss of factors such as 53BP1, RIF1, and REV7/MAD2L2, which inhibit end resection of DNA double-strand breaks (DSBs). …”

A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice. by Pai, C, Deegan, R, Subramanian, L, Gal, C, Sarkar, S, Blaikley, E, Walker, C, Hulme, L, Bernhard, E, Codlin, S, Bähler, J, Allshire, R, Whitehall, S, Humphrey, T

“…DNA double-strand break (DSB) repair is a highly regulated process performed predominantly by non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. How these pathways are coordinated in the context of chromatin is unclear. …”

miR-182-mediated downregulation of BRCA1 impacts DNA repair and sensitivity to PARP inhibitors. by Moskwa, P, Buffa, F, Pan, Y, Panchakshari, R, Gottipati, P, Muschel, R, Beech, J, Kulshrestha, R, Abdelmohsen, K, Weinstock, D, Gorospe, M, Harris, A, Helleday, T, Chowdhury, D

“…Antagonizing miR-182 enhances BRCA1 protein levels and protects them from IR-induced cell death, while overexpressing miR-182 reduces BRCA1 protein, impairs homologous recombination-mediated repair, and render cells hypersensitive to IR. …”

Inhibition of CD4/CDK6 enhances radiosensitivity of HPV negative head and neck squamous cell carcinomas by Göttgens, E, Bussink, J, Leszczynska, K, Peters, H, Span, P, Hammond, E

“…<br/><br/> <strong>Methods and Materials</strong> The effect of palbociclib on radiosensitivity was determined in HPV-ve and +ve HNSCC cell lines using colony survival assays, immunofluorescent staining of repair proteins, homologous recombination (HR) assays, cell cycle and metaphase spread analyses. …”

Enhanced CD8+ T cell immune responses and protection elicited against Plasmodium berghei malaria by prime boost immunization regimens using a novel attenuated fowlpox virus. by Anderson, R, Hannan, C, Gilbert, S, Laidlaw, S, Sheu, E, Korten, S, Sinden, R, Butcher, G, Skinner, M, Hill, A

“…The gene encoding the P. berghei circumsporozoite (PbCS) protein was inserted into an FPV vaccine strain licensed for use in chickens, Webster's FPV, and the novel FPV vaccine strain FP9 by homologous recombination. The novel FP9 strain proved more potent as a vaccine for eliciting CD8(+) T cell responses against the PbCS Ag. …”

Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance by Secrier, M, Li, X, De Silva, N, Eldridge, MD, Contino, G, Bornschein, J, Macrae, S, Grehan, N, O'Donovan, M, Miremadi, A, Yang, T-P, Bower, L, Chettouh, H, Crawte, J, Galeano-Dalmau, N, Grabowska, A, Saunders, J, Underwood, T, Waddell, N, Barbour, AP, Nutzinger, B, Achilleos, A, Edwards, PAW, Lynch, AG, Tavaré, S, Fitzgerald, RC, Oesophageal Cancer Clinical And Molecular Stratification (Occams) Consortium

“…However, mutational signatures showed three distinct molecular subtypes with potential therapeutic relevance, which we verified in an independent cohort (n = 87): (i) enrichment for BRCA signature with prevalent defects in the homologous recombination pathway; (ii) dominant T&gt;G mutational pattern associated with a high mutational load and neoantigen burden; and (iii) C&gt;A/T mutational pattern with evidence of an aging imprint. …”

Evaluation of pEASY-uni seamless cloning and assembly kit to clone multiple fragments of Elaeis guineensis DNA by Moradpour, Mahdi, Abdulah, Siti Nor Akmar

“…Several seamless DNA assembly kits based on in vitro homologous recombination activity are commercially available in recent years for efficient and rapid construction of expression vectors, subsequent transformation and investigation of gene functionality. …”
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Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii by Lola Esland, Marco Larrea-Alvarez, Saul Purton

“…Expression of foreign genes in the chloroplast is particularly advantageous as: (i) accumulation of product in this sub-cellular compartment minimises potential toxicity to the rest of the cell; (ii) genes can integrate at specific loci of the chloroplast genome (plastome) by homologous recombination; (iii) the high ploidy of the plastome and the high-level expression of chloroplast genes can be exploited to achieve levels of recombinant protein as high as 5% total cell protein; (iv) the lack of any gene silencing mechanisms in the chloroplast ensures stable expression of transgenes. …”
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Construction and characterisation of a complete reverse genetics system of dengue virus type 3 by Jefferson Jose da Silva Santos, Marli Tenorio Cordeiro, Giovani Rota Bertani, Ernesto Torres de Azevedo Marques, Laura Helena Vega Gonzales Gil

“…Both constructs were assembled into a newly designed yeast- E. coli shuttle vector by homologous recombination technique and propagated in yeast to prevent any possible genome instability in E. coli . …”
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The role of aberrant DNA methylation in cancer initiation and clinical impacts by Franziska Geissler, Ksenija Nesic, Olga Kondrashova, Alexander Dobrovic, Elizabeth M. Swisher, Clare L. Scott, Matthew J. Wakefield

“…These include the mismatch DNA repair genes MLH1 and MSH2 , homologous recombination DNA repair genes BRCA1 and RAD51C , the TERT oncogene and genes within the P15/P16/RB1/E2F tumour suppressor axis. …”
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Alternative Lengthening of Telomeres in Yeast: Old Questions and New Approaches by Kendra Musmaker, Jacob Wells, Meng-Chia Tsai, Josep M. Comeron, Anna Malkova

“…Alternative lengthening of telomeres (ALT) is a homologous recombination-based pathway utilized by 10–15% of cancer cells that allows cells to maintain their telomeres in the absence of telomerase. …”
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Establishment of a sheep immortalization cell line for generating and amplifying Orf virus recombinants by Shihui Sun, Kui Zhao, Huijun Lu, Xingyuan Liu, Yue Li, Qi Li, Deguang Song, Yungang Lan, Wenqi He, Feng Gao, Zi Li, Jiyu Guan

“…Importantly, immortalized OFTu cell line was suitable for generating gene-modified ORFV recombinant through homologous recombination, and for the amplification of ORFV recombinant. …”
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Maintenance therapy for recurrent epithelial ovarian cancer: current therapies and future perspectives – a review by Sudeep Gupta, Shona Nag, Shyam Aggarwal, Amit Rauthan, Narayanankutty Warrier

“…PARPi have also been found to act regardless of BRCA status or homologous recombination (HR) deficiency. Several trials testing PARPi early in maintenance therapy are in progress and their results will shed light on the optimal timing of maintenance therapy that gives the most benefit with least toxicity. …”
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The Dual PI3K/mTOR Inhibitor NVP-BEZ235 Is a Potent Inhibitor of ATM- and DNA-PKCs-Mediated DNA Damage Responses by Bipasha Mukherjee, Nozomi Tomimatsu, Kaushik Amancherla, Cristel V. Camacho, Nandini Pichamoorthy, Sandeep Burma

“…Consequently, NVP-BEZ235 blocks both nonhomologous end joining and homologous recombination DNA repair pathways resulting in significant attenuation of DSB repair. …”
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Emergence of trait variability through the lens of nitrogen assimilation in Prochlorococcus by Paul M Berube, Anna Rasmussen, Rogier Braakman, Ramunas Stepanauskas, Sallie W Chisholm

“…The distribution of nitrate assimilation genes within clades appears largely governed by vertical inheritance, gene loss, and homologous recombination. By mapping this process onto a model of Prochlorococcus’ macroevolution, we propose that niche-constructing adaptive radiations and subsequent niche partitioning set the stage for loss of nitrate assimilation genes from basal lineages as they specialized to lower light levels. …”
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BRCA Mutations and Fertility Preservation by Joana Dias Nunes, Isabelle Demeestere, Melody Devos

“…<i>BRCA1</i> and <i>BRCA2</i> act as tumor suppressor genes as they are key regulators of DNA repair through homologous recombination. Evidence of the accumulation of DNA double-strand break has been reported in aging oocytes, while <i>BRCA</i> expression decreases, leading to the hypothesis that <i>BRCA</i> mutation may impact fertility. …”
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DNA Damage Response Alterations in Ovarian Cancer: From Molecular Mechanisms to Therapeutic Opportunities by María Ovejero-Sánchez, Rogelio González-Sarmiento, Ana Belén Herrero

“…It is known that approximately half of the most frequent subtype, high-grade serous carcinoma (HGSC), exhibit defects in DNA double-strand break (DSB) repair by homologous recombination (HR), and current evidence indicates that probably all HGSCs harbor a defect in at least one DDR pathway. …”
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Small-Molecule Inhibitors Targeting FEN1 for Cancer Therapy by Fan Yang, Zhigang Hu, Zhigang Guo

“…Several studies have implicated FEN1 in the regulation of other DNA repair pathways, including homologous recombination repair (HRR) and non-homologous end joining (NHEJ). …”
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Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism by REN Junle, GUO Xiaopeng, LEI Cairong, ZHANG Miaomiao, CHAI Ran, LU Dong

“…Existing studies have demonstrated that homologous end joining, homologous recombination, single-strand annealing, and selective end joining play critical roles in the repair of DNA DSBs. …”
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