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1181
Targeting BRCA1 and BRCA2 deficiencies with G-quadruplex-interacting compounds
Published 2016“…Stalled replication forks can be stabilized and restarted by homologous recombination (HR), which also repairs DNA double-strand breaks (DSBs) arising at collapsed forks. …”
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1182
The CST complex mediates end protection at double-strand breaks and promotes PARP inhibitor sensitivity in BRCA1-deficient cells
Published 2018“…This interaction is counteracted by the restoration of BRCA1-independent homologous recombination through loss of factors such as 53BP1, RIF1, and REV7/MAD2L2, which inhibit end resection of DNA double-strand breaks (DSBs). …”
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1183
A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.
Published 2014“…DNA double-strand break (DSB) repair is a highly regulated process performed predominantly by non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. How these pathways are coordinated in the context of chromatin is unclear. …”
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1184
miR-182-mediated downregulation of BRCA1 impacts DNA repair and sensitivity to PARP inhibitors.
Published 2011“…Antagonizing miR-182 enhances BRCA1 protein levels and protects them from IR-induced cell death, while overexpressing miR-182 reduces BRCA1 protein, impairs homologous recombination-mediated repair, and render cells hypersensitive to IR. …”
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1185
Inhibition of CD4/CDK6 enhances radiosensitivity of HPV negative head and neck squamous cell carcinomas
Published 2019“…<br/><br/> <strong>Methods and Materials</strong> The effect of palbociclib on radiosensitivity was determined in HPV-ve and +ve HNSCC cell lines using colony survival assays, immunofluorescent staining of repair proteins, homologous recombination (HR) assays, cell cycle and metaphase spread analyses. …”
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1186
Enhanced CD8+ T cell immune responses and protection elicited against Plasmodium berghei malaria by prime boost immunization regimens using a novel attenuated fowlpox virus.
Published 2004“…The gene encoding the P. berghei circumsporozoite (PbCS) protein was inserted into an FPV vaccine strain licensed for use in chickens, Webster's FPV, and the novel FPV vaccine strain FP9 by homologous recombination. The novel FP9 strain proved more potent as a vaccine for eliciting CD8(+) T cell responses against the PbCS Ag. …”
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1187
Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance
Published 2016“…However, mutational signatures showed three distinct molecular subtypes with potential therapeutic relevance, which we verified in an independent cohort (n = 87): (i) enrichment for BRCA signature with prevalent defects in the homologous recombination pathway; (ii) dominant T>G mutational pattern associated with a high mutational load and neoantigen burden; and (iii) C>A/T mutational pattern with evidence of an aging imprint. …”
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1188
Evaluation of pEASY-uni seamless cloning and assembly kit to clone multiple fragments of Elaeis guineensis DNA
Published 2017“…Several seamless DNA assembly kits based on in vitro homologous recombination activity are commercially available in recent years for efficient and rapid construction of expression vectors, subsequent transformation and investigation of gene functionality. …”
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1189
Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii
Published 2018-10-01“…Expression of foreign genes in the chloroplast is particularly advantageous as: (i) accumulation of product in this sub-cellular compartment minimises potential toxicity to the rest of the cell; (ii) genes can integrate at specific loci of the chloroplast genome (plastome) by homologous recombination; (iii) the high ploidy of the plastome and the high-level expression of chloroplast genes can be exploited to achieve levels of recombinant protein as high as 5% total cell protein; (iv) the lack of any gene silencing mechanisms in the chloroplast ensures stable expression of transgenes. …”
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1190
Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Published 2013-12-01“…Both constructs were assembled into a newly designed yeast- E. coli shuttle vector by homologous recombination technique and propagated in yeast to prevent any possible genome instability in E. coli . …”
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1191
The role of aberrant DNA methylation in cancer initiation and clinical impacts
Published 2024-01-01“…These include the mismatch DNA repair genes MLH1 and MSH2 , homologous recombination DNA repair genes BRCA1 and RAD51C , the TERT oncogene and genes within the P15/P16/RB1/E2F tumour suppressor axis. …”
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1192
Alternative Lengthening of Telomeres in Yeast: Old Questions and New Approaches
Published 2024-01-01“…Alternative lengthening of telomeres (ALT) is a homologous recombination-based pathway utilized by 10–15% of cancer cells that allows cells to maintain their telomeres in the absence of telomerase. …”
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1193
Establishment of a sheep immortalization cell line for generating and amplifying Orf virus recombinants
Published 2022-12-01“…Importantly, immortalized OFTu cell line was suitable for generating gene-modified ORFV recombinant through homologous recombination, and for the amplification of ORFV recombinant. …”
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1194
Maintenance therapy for recurrent epithelial ovarian cancer: current therapies and future perspectives – a review
Published 2019-11-01“…PARPi have also been found to act regardless of BRCA status or homologous recombination (HR) deficiency. Several trials testing PARPi early in maintenance therapy are in progress and their results will shed light on the optimal timing of maintenance therapy that gives the most benefit with least toxicity. …”
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1195
The Dual PI3K/mTOR Inhibitor NVP-BEZ235 Is a Potent Inhibitor of ATM- and DNA-PKCs-Mediated DNA Damage Responses
Published 2012-01-01“…Consequently, NVP-BEZ235 blocks both nonhomologous end joining and homologous recombination DNA repair pathways resulting in significant attenuation of DSB repair. …”
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1196
Emergence of trait variability through the lens of nitrogen assimilation in Prochlorococcus
Published 2019-02-01“…The distribution of nitrate assimilation genes within clades appears largely governed by vertical inheritance, gene loss, and homologous recombination. By mapping this process onto a model of Prochlorococcus’ macroevolution, we propose that niche-constructing adaptive radiations and subsequent niche partitioning set the stage for loss of nitrate assimilation genes from basal lineages as they specialized to lower light levels. …”
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1197
BRCA Mutations and Fertility Preservation
Published 2023-12-01“…<i>BRCA1</i> and <i>BRCA2</i> act as tumor suppressor genes as they are key regulators of DNA repair through homologous recombination. Evidence of the accumulation of DNA double-strand break has been reported in aging oocytes, while <i>BRCA</i> expression decreases, leading to the hypothesis that <i>BRCA</i> mutation may impact fertility. …”
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1198
DNA Damage Response Alterations in Ovarian Cancer: From Molecular Mechanisms to Therapeutic Opportunities
Published 2023-01-01“…It is known that approximately half of the most frequent subtype, high-grade serous carcinoma (HGSC), exhibit defects in DNA double-strand break (DSB) repair by homologous recombination (HR), and current evidence indicates that probably all HGSCs harbor a defect in at least one DDR pathway. …”
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1199
Small-Molecule Inhibitors Targeting FEN1 for Cancer Therapy
Published 2022-07-01“…Several studies have implicated FEN1 in the regulation of other DNA repair pathways, including homologous recombination repair (HRR) and non-homologous end joining (NHEJ). …”
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1200
Progress of research on double-strand break damage induced by heavy-ion beam radiation and repair mechanism
Published 2023-06-01“…Existing studies have demonstrated that homologous end joining, homologous recombination, single-strand annealing, and selective end joining play critical roles in the repair of DNA DSBs. …”
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