-
1201
Pre-Existing and Acquired Resistance to PARP Inhibitor-Induced Synthetic Lethality
Published 2022-11-01“…Such treatment has been effectively employed against homologous recombination-defective solid tumors as well as hematopoietic malignancies. …”
Get full text
Article -
1202
Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia
Published 2022-01-01“…To mimic both the dietary restrictions and metabolic decompensation seen in MMA patients, we developed a novel protein-controlled diet regimen in a Mmut deficient mouse model of MMA and demonstrated the therapeutic benefit of mLB-001, a nuclease-free, promoterless recombinant AAV GeneRideTM vector designed to insert the mouse Mmut into the endogenous albumin locus via homologous recombination. A single intravenous administration of mLB-001 to neonatal or adult MMA mice prevented body weight loss and mortality when challenged with a high protein diet. …”
Get full text
Article -
1203
A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast
Published 2003-04-01“…The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and a TAR vector that has short (~ 60 bp) 5' and 3' gene targeting sequences (hooks).…”
Get full text
Article -
1204
Combined PARP Inhibition and Immune Checkpoint Therapy in Solid Tumors
Published 2020-06-01“…PARPi are active across a range of tumor types harboring <i>BRCA</i> mutations and also <i>BRCA</i>-negative cancers, such as ovarian, breast or prostate cancers with homologous recombination deficiencies (HRD). Depending on immune contexture, immune checkpoint inhibitors (ICIs), such as anti-PD1/PD-L1 and anti-CTLA-4, elicit potent antitumor effects and have been approved in various cancers types. …”
Get full text
Article -
1205
Role of PARP Inhibitors in Cancer Immunotherapy: Potential Friends to Immune Activating Molecules and Foes to Immune Checkpoints
Published 2022-11-01“…Poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) induce cytotoxic effects as single agents in tumors characterized by defective repair of DNA double-strand breaks deriving from BRCA1/2 mutations or other abnormalities in genes associated with homologous recombination. Preclinical studies have shown that PARPi-induced DNA damage may affect the tumor immune microenvironment and immune-mediated anti-tumor response through several mechanisms. …”
Get full text
Article -
1206
A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
Published 2023-02-01“…Adeno-associated viruses (AAV) act as donor DNA for homologous recombination in infected cells, including rodent embryos. …”
Get full text
Article -
1207
SMARCAL1 ubiquitylation controls its association with RPA-coated ssDNA and promotes replication fork stability.
Published 2024-03-01“…RFWD3, a recently described Fanconi anemia (FA) ubiquitin ligase, associates with RPA and promotes its ubiquitylation, facilitating late steps of homologous recombination (HR). Intriguingly, RFWD3 also regulates fork progression, restart and stability via poorly understood mechanisms. …”
Get full text
Article -
1208
Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2.
Published 2013-01-01“…In higher eukaryotes, RAD51 functions as an essential protein in homologous recombination and recombinational repair of DNA double strand breaks. …”
Get full text
Article -
1209
PARP Inhibitors: Strategic Use and Optimal Management in Ovarian Cancer
Published 2024-02-01“…Discovered in the 1980s, PARP inhibitors (PARPis) were initially developed to exploit the presence of BRCA mutations, which disrupt the homologous recombination repair of deoxyribonucleic acid (DNA) via synthetic lethality, an intrinsic vulnerability caused by the cell’s dependence on other DNA repair mechanisms for which PARP is an essential contributor. …”
Get full text
Article -
1210
When Order Meets Disorder: Modeling and Function of the Protein Interface in Fuzzy Complexes
Published 2021-10-01“…The discussion focusses on two specific cases, microtubules and homologous recombination nucleoprotein filaments, where a network of intrinsically disordered tails exerts regulatory function in recruiting partner macromolecules, proteins or DNA and tuning the atomic level association. …”
Get full text
Article -
1211
Reduced sister chromatid cohesion acts as a tumor penetrance modifier.
Published 2022-08-01“…Interestingly, SCC also plays a role in homologous recombination, and we did observe elevated levels of mitotic recombination derived p53 LOH in tumors from esco2 haploinsufficient animals; as well as elevated levels of mitotic recombination throughout the genome of human ESCO2 deficient tumors. …”
Get full text
Article -
1212
A mutant of listeria monocytogenes shows decreased virulence and confers protection against listeriosis in mice
Published 2020-02-01“…This research was performed to obtain a safe and highly immunogenic Listeria strain and evaluate the biological characteristics of the deletion mutant. Based on homologous recombination technology, we constructed a deletion mutant Lm90-ΔinlB of L. monocytogenes. …”
Get full text
Article -
1213
Novel Therapeutic Approaches with DNA Damage Response Inhibitors for Melanoma Treatment
Published 2022-04-01“…Melanoma is characterized by homologous recombination (HR) and DNA damage response (DDR) gene mutations and by high replicative stress, which increase the endogenous DNA damage, leading to the activation of DDR. …”
Get full text
Article -
1214
Parp Inhibitors and Radiotherapy: A New Combination for Prostate Cancer (Systematic Review)
Published 2023-08-01“…Two double-stranded DNA breaks (DSB) repair pathways: (1) non-homologous end-joining (NHEJ) and (2) homologous recombination (HR); and (3) a single-stranded DNA break (SSB) repair pathway (base excision repair, BER). …”
Get full text
Article -
1215
Progress and prospects in research and clinical practice of hormone receptor-positive, HER-2-negative breast cancer with BRCA1/2 mutations
Published 2023-06-01“…Hormone receptor (HR)-positive, human epidermal growth factor receptor type 2 (HER2)-negative (HR+/HER2-) BC with BRCA1 and/or BRCA2 mutations (BRCA1/2m) is a unique BC subset with dual drivers: homologous recombination deficiency and hormone receptor signaling. …”
Get full text
Article -
1216
Recombinant adenovirus-mediated intestinal trefoil factor gene therapy for burn-induced intestinal mucosal injury.
Published 2013-01-01“…First, a recombinant shuttle plasmid was constructed by ligating a pAdTrack-CMV vector with a full-length hITF gene containing a signal peptide and the mature peptide, followed by the recombinant Ad-hITF adenovirus vector after linearization and homologous recombination with the backbone plasmid in the competent BJ5183 strain. …”
Get full text
Article -
1217
Genome Maintenance by DNA Helicase B
Published 2020-05-01“…HELB also inhibits homologous recombination by reducing long-range end resection. …”
Get full text
Article -
1218
Extreme Polyploidy of Carsonella, an Organelle-Like Bacterium with a Drastically Reduced Genome
Published 2022-06-01“…The basis of polyploidy of Carsonella is unknown, but it potentially plays a role in the repair of DNA damage through homologous recombination. IMPORTANCE Mitochondria and plastids are endosymbiotic organelles in eukaryotic cells and are derived from free-living bacteria. …”
Get full text
Article -
1219
Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia.
Published 2022-01-01“…To mimic both the dietary restrictions and metabolic decompensation seen in MMA patients, we developed a novel protein-controlled diet regimen in a Mmut deficient mouse model of MMA and demonstrated the therapeutic benefit of mLB-001, a nuclease-free, promoterless recombinant AAV GeneRideTM vector designed to insert the mouse Mmut into the endogenous albumin locus via homologous recombination. A single intravenous administration of mLB-001 to neonatal or adult MMA mice prevented body weight loss and mortality when challenged with a high protein diet. …”
Get full text
Article -
1220
Changing the History of Prostate Cancer with New Targeted Therapies
Published 2021-04-01“…Approximately 20–25% of mCRPCs have somatic or germline alterations in DNA repair genes involved in homologous recombination. These subtypes are usually associated with more aggressive disease. …”
Get full text
Article