Pre-Existing and Acquired Resistance to PARP Inhibitor-Induced Synthetic Lethality by Bac Viet Le, Paulina Podszywałow-Bartnicka, Katarzyna Piwocka, Tomasz Skorski

“…Such treatment has been effectively employed against homologous recombination-defective solid tumors as well as hematopoietic malignancies. …”
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Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia by Shengwen Zhang, Amy Bastille, Susana Gordo, Nikhil Ramesh, Jenisha Vora, Elizabeth McCarthy, Xiaohan Zhang, Dylan Frank, Chih-Wei Ko, Carmen Wu, Noel Walsh, Shreya Amarwani, Jing Liao, Qiang Xiong, Lauren Drouin, Matthias Hebben, Kyle Chiang, B. Nelson Chau

“…To mimic both the dietary restrictions and metabolic decompensation seen in MMA patients, we developed a novel protein-controlled diet regimen in a Mmut deficient mouse model of MMA and demonstrated the therapeutic benefit of mLB-001, a nuclease-free, promoterless recombinant AAV GeneRideTM vector designed to insert the mouse Mmut into the endogenous albumin locus via homologous recombination. A single intravenous administration of mLB-001 to neonatal or adult MMA mice prevented body weight loss and mortality when challenged with a high protein diet. …”
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A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast by Barrett J Carl, Ouspenski Ilia, Leem Sun-Hee, Kouprina Natalay, Noskov Vladimir N, Larionov Vladimir

“…The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and a TAR vector that has short (~ 60 bp) 5' and 3' gene targeting sequences (hooks).…”
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Combined PARP Inhibition and Immune Checkpoint Therapy in Solid Tumors by Florent Peyraud, Antoine Italiano

“…PARPi are active across a range of tumor types harboring <i>BRCA</i> mutations and also <i>BRCA</i>-negative cancers, such as ovarian, breast or prostate cancers with homologous recombination deficiencies (HRD). Depending on immune contexture, immune checkpoint inhibitors (ICIs), such as anti-PD1/PD-L1 and anti-CTLA-4, elicit potent antitumor effects and have been approved in various cancers types. …”
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Role of PARP Inhibitors in Cancer Immunotherapy: Potential Friends to Immune Activating Molecules and Foes to Immune Checkpoints by Ornella Franzese, Grazia Graziani

“…Poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) induce cytotoxic effects as single agents in tumors characterized by defective repair of DNA double-strand breaks deriving from BRCA1/2 mutations or other abnormalities in genes associated with homologous recombination. Preclinical studies have shown that PARPi-induced DNA damage may affect the tumor immune microenvironment and immune-mediated anti-tumor response through several mechanisms. …”
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A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes by Manabu Abe, Ena Nakatsukasa, Rie Natsume, Shun Hamada, Kenji Sakimura, Ayako M. Watabe, Toshihisa Ohtsuka

“…Adeno-associated viruses (AAV) act as donor DNA for homologous recombination in infected cells, including rodent embryos. …”
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SMARCAL1 ubiquitylation controls its association with RPA-coated ssDNA and promotes replication fork stability. by Maïlyn Yates, Isabelle Marois, Edlie St-Hilaire, Daryl A Ronato, Billel Djerir, Chloé Brochu, Théo Morin, Ian Hammond-Martel, Sari Gezzar-Dandashi, Lisa Casimir, Elliot Drobetsky, Laurent Cappadocia, Jean-Yves Masson, Hugo Wurtele, Alexandre Maréchal

“…RFWD3, a recently described Fanconi anemia (FA) ubiquitin ligase, associates with RPA and promotes its ubiquitylation, facilitating late steps of homologous recombination (HR). Intriguingly, RFWD3 also regulates fork progression, restart and stability via poorly understood mechanisms. …”
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Homologous pairing activities of two rice RAD51 proteins, RAD51A1 and RAD51A2. by Yuichi Morozumi, Ryohei Ino, Shukuko Ikawa, Naozumi Mimida, Takeshi Shimizu, Seiichi Toki, Hiroaki Ichikawa, Takehiko Shibata, Hitoshi Kurumizaka

“…In higher eukaryotes, RAD51 functions as an essential protein in homologous recombination and recombinational repair of DNA double strand breaks. …”
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PARP Inhibitors: Strategic Use and Optimal Management in Ovarian Cancer by Nicholas Hirschl, Wildnese Leveque, Julia Granitto, Valia Sammarco, Mervyns Fontillas, Richard T. Penson

“…Discovered in the 1980s, PARP inhibitors (PARPis) were initially developed to exploit the presence of BRCA mutations, which disrupt the homologous recombination repair of deoxyribonucleic acid (DNA) via synthetic lethality, an intrinsic vulnerability caused by the cell’s dependence on other DNA repair mechanisms for which PARP is an essential contributor. …”
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When Order Meets Disorder: Modeling and Function of the Protein Interface in Fuzzy Complexes by Sophie Sacquin-Mora, Chantal Prévost

“…The discussion focusses on two specific cases, microtubules and homologous recombination nucleoprotein filaments, where a network of intrinsically disordered tails exerts regulatory function in recruiting partner macromolecules, proteins or DNA and tuning the atomic level association. …”
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Reduced sister chromatid cohesion acts as a tumor penetrance modifier. by Jun Wang, Holly R Thomas, Yu Chen, Stefanie M Percival, Stephanie C Waldrep, Ryne C Ramaker, Robert G Thompson, Sara J Cooper, Zechen Chong, John M Parant

“…Interestingly, SCC also plays a role in homologous recombination, and we did observe elevated levels of mitotic recombination derived p53 LOH in tumors from esco2 haploinsufficient animals; as well as elevated levels of mitotic recombination throughout the genome of human ESCO2 deficient tumors. …”
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A mutant of listeria monocytogenes shows decreased virulence and confers protection against listeriosis in mice by Jingjing REN, Mingwei YANG, Pengyan WANG, Jianjun JIANG, Genqiang YAN

“…This research was performed to obtain a safe and highly immunogenic Listeria strain and evaluate the biological characteristics of the deletion mutant. Based on homologous recombination technology, we constructed a deletion mutant Lm90-ΔinlB of L. monocytogenes. …”
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Novel Therapeutic Approaches with DNA Damage Response Inhibitors for Melanoma Treatment by Luisa Maresca, Barbara Stecca, Laura Carrassa

“…Melanoma is characterized by homologous recombination (HR) and DNA damage response (DDR) gene mutations and by high replicative stress, which increase the endogenous DNA damage, leading to the activation of DDR. …”
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Parp Inhibitors and Radiotherapy: A New Combination for Prostate Cancer (Systematic Review) by Inés Rivero Belenchón, Carmen Belen Congregado Ruiz, Carmen Saez, Ignacio Osman García, Rafael Antonio Medina López

“…Two double-stranded DNA breaks (DSB) repair pathways: (1) non-homologous end-joining (NHEJ) and (2) homologous recombination (HR); and (3) a single-stranded DNA break (SSB) repair pathway (base excision repair, BER). …”
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Progress and prospects in research and clinical practice of hormone receptor-positive, HER-2-negative breast cancer with BRCA1/2 mutations by Shunchao Yan, Murshid Imam

“…Hormone receptor (HR)-positive, human epidermal growth factor receptor type 2 (HER2)-negative (HR+/HER2-) BC with BRCA1 and/or BRCA2 mutations (BRCA1/2m) is a unique BC subset with dual drivers: homologous recombination deficiency and hormone receptor signaling. …”
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Recombinant adenovirus-mediated intestinal trefoil factor gene therapy for burn-induced intestinal mucosal injury. by Yong Sun, Yun Zhu, Liangxi Wang, Xuefei Mao, Xi Peng, Yizhi Peng

“…First, a recombinant shuttle plasmid was constructed by ligating a pAdTrack-CMV vector with a full-length hITF gene containing a signal peptide and the mature peptide, followed by the recombinant Ad-hITF adenovirus vector after linearization and homologous recombination with the backbone plasmid in the competent BJ5183 strain. …”
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Genome Maintenance by DNA Helicase B by Lindsey Hazeslip, Maroof Khan Zafar, Muhammad Zain Chauhan, Alicia K. Byrd

“…HELB also inhibits homologous recombination by reducing long-range end resection. …”
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Extreme Polyploidy of Carsonella, an Organelle-Like Bacterium with a Drastically Reduced Genome by Atsushi Nakabachi, Nancy A. Moran

“…The basis of polyploidy of Carsonella is unknown, but it potentially plays a role in the repair of DNA damage through homologous recombination. IMPORTANCE Mitochondria and plastids are endosymbiotic organelles in eukaryotic cells and are derived from free-living bacteria. …”
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Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia. by Shengwen Zhang, Amy Bastille, Susana Gordo, Nikhil Ramesh, Jenisha Vora, Elizabeth McCarthy, Xiaohan Zhang, Dylan Frank, Chih-Wei Ko, Carmen Wu, Noel Walsh, Shreya Amarwani, Jing Liao, Qiang Xiong, Lauren Drouin, Matthias Hebben, Kyle Chiang, B Nelson Chau

“…To mimic both the dietary restrictions and metabolic decompensation seen in MMA patients, we developed a novel protein-controlled diet regimen in a Mmut deficient mouse model of MMA and demonstrated the therapeutic benefit of mLB-001, a nuclease-free, promoterless recombinant AAV GeneRideTM vector designed to insert the mouse Mmut into the endogenous albumin locus via homologous recombination. A single intravenous administration of mLB-001 to neonatal or adult MMA mice prevented body weight loss and mortality when challenged with a high protein diet. …”
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Changing the History of Prostate Cancer with New Targeted Therapies by Susana Hernando Polo, Diana Moreno Muñoz, Adriana Carolina Rosero Rodríguez, Jorge Silva Ruiz, Diana Isabel Rosero Rodríguez, Felipe Couñago

“…Approximately 20–25% of mCRPCs have somatic or germline alterations in DNA repair genes involved in homologous recombination. These subtypes are usually associated with more aggressive disease. …”
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