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1221
Selective induction of DNA repair pathways in human B cells activated by CD4+ T cells.
Published 2010-12-01“…We first demonstrated that several DNA mismatch repair, homologous recombination, base excision repair, and ATR signaling genes were overexpressed in GC B cells relative to naïve and memory B cells, reflecting activation of a process we have termed somatic hyperrepair (SHR). …”
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1222
Improving homology‐directed repair by small molecule agents for genetic engineering in unconventional yeast?—Learning from the engineering of mammalian systems
Published 2024-02-01“…In many unconventional yeasts, such as those promising new hosts for cell factory design but also human pathogenic yeasts and food spoilers, this progress has been limited by the fact that most yeasts favour non‐homologous end joining (NHEJ) over homologous recombination (HR) as a DNA repair mechanism, impairing genetic access to these hosts. …”
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1223
Sequence features of <it>HLA-DRB1 </it>locus define putative basis for gene conversion and point mutations
Published 2008-05-01“…</p> <p>Conclusion</p> <p>The identified sequence features could putatively act in recombination allowing either less (CpG dinucleotides) or more specific DNA cleavage (complex sequences) or homologous recombination (complex sequences).</p>…”
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1224
Multi-Gene Testing Overview with a Clinical Perspective in Metastatic Triple-Negative Breast Cancer
Published 2021-07-01“…We make an overview of the clinical utility of multi-gene testing in mTNBC, and then, as immunotherapy is emerging as a possible targeted therapy for mTNBC, we also briefly report on the results of the latest clinical trials involving immune checkpoint inhibitors (ICIs) and TNBC, where NGS could play a role for the potential predictive utility of homologous recombination repair deficiency (HRD) and tumor mutational burden (TMB).…”
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1225
Therapeutic Potential of PARP Inhibitors in the Treatment of Metastatic Castration-Resistant Prostate Cancer
Published 2020-11-01“…Up to 30% of patients with mCRPC have mutations in homologous recombination repair (HRR) genes. Poly (ADP-ribose) polymerase (PARP) inhibitors take advantage of HRR deficiency to kill tumor cells based on the concept of synthetic lethality. …”
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1226
p53 Controls Meiotic Prophase Progression and Crossover Formation
Published 2022-08-01“…To avoid genomic instability, these DSBs need to be correctly repaired by homologous recombination. Surveillance mechanisms involving the DNA damage response (DDR) pathway ATM-CHK2-p53 can detect the persistence of unrepaired DBSs and activate the recombination-dependent arrest at the pachytene stage. …”
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1227
Interrogating the Genomic Landscape of Uterine Leiomyosarcoma: A Potential for Patient Benefit
Published 2022-03-01“…Recent identification of deficiencies in homologous recombination in a minority of these tumours, however, has provided a rationale for investigation of PARP inhibitors in this sub-set. …”
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1228
Evolutionary diversification of an ancient gene family (<it>rhs</it>) through C-terminal displacement
Published 2009-12-01“…Furthermore, we demonstrate that Rhs 'core' domains and variable C-termini are evolutionarily decoupled, and propose that <it>rhs </it>diversity is driven by homologous recombination with circular intermediates. Existing C-termini are displaced by laterally acquired alternatives, creating long arrays of dissociated 'tips' that characterize the appearance of <it>rhs </it>loci.…”
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1229
Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation
Published 2023-05-01“…This review summarizes several strategies for editing of mycoplasma genomes, including homologous recombination, transposons, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, and synthetic biology. …”
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1230
Genome-wide analysis of DNA-PK-bound MRN cleavage products supports a sequential model of DSB repair pathway choice
Published 2023-09-01“…Abstract The Mre11-Rad50-Nbs1 (MRN) complex recognizes and processes DNA double-strand breaks for homologous recombination by performing short-range removal of 5ʹ strands. …”
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1231
Chromosome Instability in Fanconi Anemia: From Breaks to Phenotypic Consequences
Published 2020-12-01“…This pathway regulates the repair of DNA interstrand crosslinks (ICLs) through homologous recombination. In FA proper repair of ICLs is impaired and accumulation of toxic DNA double strand breaks occurs. …”
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1232
Size of gene specific inverted repeat--dependent gene deletion In Saccharomyces cerevisiae.
Published 2013-01-01“…The inherent instability of the inverted sequences in close proximity facilitates the self-excision of the entire mutagenesis cassette deposited in the genome and promotes homologous recombination resulting in a seamless deletion via a single transformation. …”
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1233
Facing inevitable PARPis resistance: Mechanisms and therapeutic strategies for breast cancer treatment
Published 2023-04-01“…Abstract BRCA1/2 gene mutations, which result in a dysfunction of homologous recombination repair, have been discovered in at least 5% of breast cancer (BC) patients with the increase in BC incidence in recent years. …”
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1234
Resistance to DNA repair inhibitors in cancer
Published 2022-11-01“…This is exemplified by PARP inhibitors (PARPi) used to treat cancers with defects in the homologous recombination DDR pathway. A series of novel DDR targets are now also under pre‐clinical or clinical investigation, including inhibitors of ATR kinase, WRN helicase or the DNA polymerase/helicase Polθ (Pol‐Theta). …”
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1235
Exploring the evolutionary dynamics of plasmids: the <it>Acinetobacter </it>pan-plasmidome
Published 2010-02-01“…Additionally and/or alternatively, from a genome perspective, plasmids can provide a basis for genomic rearrangements <it>via </it>homologous recombination and so they can facilitate the loss or acquisition of genes during these events, which eventually may lead to horizontal gene transfer (HGT). …”
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1236
Genetic manipulation and targeted protein degradation in mammalian systems: practical considerations, tips and tricks for discovery research
Published 2023-07-01“…Despite such apparent advantages, genetic manipulation (i.e., knock‐down, knock‐out, mutation, and tagging) in mammalian systems can be challenging due to problems with delivery, low rates of homologous recombination, and epigenetic silencing. The advent of CRISPR‐Cas9 in combination with the development of robust differentiation protocols that can efficiently generate a variety of different cell types in vitro has accelerated our ability to probe gene function in a more physiological setting. …”
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1237
Construction and expression verification of mouse spermatogenic cell-specific expression vector AAV-Dazl-RFP-Flag
Published 2020-06-01“…Methods The Adeno-associated virus vector AAV-CMV-RFP-Flag was modified by homologous recombination to construct mouse permatogenic cell-specific expression vector AAV-Dazl-RFP-Flag. …”
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1238
Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer
Published 2023-06-01“…Abstract The Holliday junction (HJ) is a DNA intermediate of homologous recombination, involved in many fundamental physiological processes. …”
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1239
Mechanistic Insights on Localized to Metastatic Prostate Cancer Transition and Therapeutic Opportunities
Published 2023-11-01“…Aberrant pathway activations of PI3K/AKT due to PTEN loss, epithelial–mesenchymal transition pathways, homologous recombination repair, and DNA repair pathway mechanisms of resistance and cross-talk lead to opportunities for therapeutic targeting in metastatic castration-resistant prostate cancer. …”
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1240
Clinicopathological and Prognostic Characteristics of RAD51 in Colorectal Cancer
Published 2020-01-01“…<i>Background and Objectives</i>: RAD51 plays an essential role in DNA repair via homologous recombination. RAD51 facilitates strand transfer between interrupted sequences and their undamaged homologies. …”
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