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1281
A broad-spectrum cloning vector that exists as both an integrated element and a free plasmid in Chlamydia trachomatis.
Published 2021-01-01“…The plasmid was modified to contain chromosomal DNA from C. trachomatis to facilitate homologous recombination. Sequences flanking incA were cloned into the pBBR1MCS-4 vector along with the GFP:CAT cassette from the pSW2-GFP chlamydial shuttle vector. …”
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1282
Systemic Therapies for Metastatic Castration-Resistant Prostate Cancer: An Updated Review
Published 2023-10-01“…Tumors with gene alterations that affect homologous recombination repair, such as BRCA1 and BRCA2 alterations, are sensitive to poly (adenosine diphosphate–ribose) polymerase (PARP) inhibitors in mCRPC. …”
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1283
Short Double-Stranded DNA (≤40-bp) Affects Repair Pathway Choice
Published 2023-07-01“…To repair ionizing radiation (IR)-induced double strand breaks (DSBs), mammalian cells primarily use canonical non-homologous end-joining (cNHEJ), the homologous recombination (HR) pathway, and the alternative non-homologous end-joining (aEJ) as a backup. …”
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1284
Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima
Published 2022-08-01“…Conclusions A new genetic transformation protocol has been established and the inclusion of hydroxyurea in the buffer during the protoplast-generation step greatly increased the creation of knockout mutants via homologous recombination. Pathogenicity assays using the GFP-mutants showed that the mutants were able to infect the host and cause disease.…”
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1285
Competitive PCR with dual fluorescent primers enhances the specificity and reproducibility of genotyping animals generated from genome editing
Published 2023-05-01“…The most widely used genome editing approach is to introduce engineered endonucleases or CRISPR/Cas system into cells or fertilized eggs to generate double-strand DNA breaks within the targeted region, leading to DNA repair through homologous recombination or non-homologous end joining (NHEJ). …”
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1286
Ku regulates the non-homologous end joining pathway choice of DNA double-strand break repair in human somatic cells.
Published 2010-02-01“…Mammals have evolved at least two genetically discrete ways to mediate DNA DSB repair: homologous recombination (HR) and non-homologous end joining (NHEJ). …”
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1287
Sir4 Deficiency Reverses Cell Senescence by Sub-Telomere Recombination
Published 2021-04-01“…Here, we report that the sub-telomere regions facilitate telomere lengthening by homologous recombination, thereby attenuating senescence in yeast <i>Saccharomyces cerevisiae</i>. …”
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1288
Characterization of the past and current duplication activities in the human 22q11.2 region
Published 2011-01-01“…<p>Abstract</p> <p>Background</p> <p>Segmental duplications (SDs) on 22q11.2 (LCR22), serve as substrates for meiotic non-allelic homologous recombination (NAHR) events resulting in several clinically significant genomic disorders.…”
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1289
The Impact of Single- and Double-Strand DNA Breaks in Human Spermatozoa on Assisted Reproduction
Published 2020-05-01“…In sperm, SSBs are irreversible, whereas DSBs can be repaired by homologous recombination, non-homologous end joining (NHEJ) and alternative NHEJ pathways. …”
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1290
Dissection of zebrafish shha function using site-specific targeting with a Cre-dependent genetic switch
Published 2017-05-01“…In this study, we generated Zwitch, a Cre-dependent invertible gene-trap cassette, enabling the establishment of conditional alleles in zebrafish by generating intronic insertions via in vivo homologous recombination. To demonstrate the utility of Zwitch, we generated a conditional sonic hedgehog a (shha) allele. …”
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1291
Imatinib enchances the sensitivity of gastrointestinal stromal tumors to topoisomerase II inhibitors
Published 2015-06-01“…This might be due to reduced ability of GISTs to repair DNA damage by homologous recombination. Imatinib-induced reduction of Rad51 recombinase might be due to increased proteasome-dependent degradation.Conclusion. …”
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1292
Roles of SETD2 in Leukemia—Transcription, DNA-Damage, and Beyond
Published 2019-02-01“…Presence of the H3K36me3 histone mark across the genome has been correlated with transcriptional activation and elongation, but also with the regulation of DNA mismatch repair, homologous recombination and alternative splicing. The role of SETD2 and the H3K36me3 histone mark in cancer is controversial. …”
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1293
DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.
Published 2014-03-01“…The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. …”
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1294
High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
Published 2017-07-01“…In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. …”
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1295
Multiple forms of atypical rearrangements generating supernumerary derivative chromosome 15
Published 2008-01-01“…Similar to the deletions found in most cases of Angelman and Prader Willi syndrome, the duplications appear to be mediated by unequal homologous recombination involving low copy repeats (LCR) that are found clustered in the region. …”
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1296
BRCA1 deficiency in triple-negative breast cancer: Protein stability as a basis for therapy
Published 2023-02-01“…Many aspects of this multifunctional protein have been revealed, including its essential role in homologous recombination repair, E3 ubiquitin ligase activity, transcriptional regulation, and apoptosis. …”
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1297
An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in <i>Saccharomyces cerevisiae</i>
Published 2023-10-01“…Based on our previous CRISPR-Cpf1 genome-editing system in <i>Saccharomyces cerevisiae</i>, the crRNA, designed for the replication origin of the CRISPR-Cpf1 plasmid, and the ssDNA, as a template for homologous recombination, were introduced for plasmid curing. …”
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1298
Inactivation of the SGS1 and EXO1 Genes Synergistically Stimulates Plasmid Integration in Yeast
Published 2005-01-01“…Different procedures used for targeted genetic manipulations are based on homologous recombination between chromosomal and exogenous DNA. …”
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1299
Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous <i>Streptomyces</i> Hosts
Published 2022-05-01“…Then, we present an efficient, markerless and multiplex large gene cluster editing method based on in vitro CRISPR/Cas9 digestion and yeast homologous recombination. With this method, we successfully engineered the thaxtomin A cluster by simultaneously replacing the native promoters of the <i>txtED</i> operon, <i>txtABH</i> operon and <i>txtC</i> gene with strong constitutive promoters, and the yield of thaxtomin A improved to 289.5 µg/mL in heterologous <i>Streptomyces coelicolor</i> M1154. …”
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1300
Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli
Published 2013-01-01“…These two copies share a 570-bp region of homology and are separated by the ampicillin resistance gene. Homologous recombination between repeated copies of incomplete spectinomycin resistance genes results in the restoration of spectinomycin resistance. …”
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