A broad-spectrum cloning vector that exists as both an integrated element and a free plasmid in Chlamydia trachomatis. by Lotisha Garvin, Rebecca Vande Voorde, Mary Dickinson, Steven Carrell, Kevin Hybiske, Daniel Rockey

“…The plasmid was modified to contain chromosomal DNA from C. trachomatis to facilitate homologous recombination. Sequences flanking incA were cloned into the pBBR1MCS-4 vector along with the GFP:CAT cassette from the pSW2-GFP chlamydial shuttle vector. …”
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Systemic Therapies for Metastatic Castration-Resistant Prostate Cancer: An Updated Review by Koji Hatano, Norio Nonomura

“…Tumors with gene alterations that affect homologous recombination repair, such as BRCA1 and BRCA2 alterations, are sensitive to poly (adenosine diphosphate–ribose) polymerase (PARP) inhibitors in mCRPC. …”

Short Double-Stranded DNA (≤40-bp) Affects Repair Pathway Choice by Zhentian Li, Ya Wang

“…To repair ionizing radiation (IR)-induced double strand breaks (DSBs), mammalian cells primarily use canonical non-homologous end-joining (cNHEJ), the homologous recombination (HR) pathway, and the alternative non-homologous end-joining (aEJ) as a backup. …”
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Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima by Heriberto Vélëz, Jonas Skytte af Sätra, Firuz Odilbekov, Salim Bourras, Larisa Garkava-Gustavsson, Kerstin Dalman

“…Conclusions A new genetic transformation protocol has been established and the inclusion of hydroxyurea in the buffer during the protoplast-generation step greatly increased the creation of knockout mutants via homologous recombination. Pathogenicity assays using the GFP-mutants showed that the mutants were able to infect the host and cause disease.…”
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Competitive PCR with dual fluorescent primers enhances the specificity and reproducibility of genotyping animals generated from genome editing by Liezhen Fu, Emily Ma, Morihiro Okada, Yuki Shibata, Yun-Bo Shi

“…The most widely used genome editing approach is to introduce engineered endonucleases or CRISPR/Cas system into cells or fertilized eggs to generate double-strand DNA breaks within the targeted region, leading to DNA repair through homologous recombination or non-homologous end joining (NHEJ). …”
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Ku regulates the non-homologous end joining pathway choice of DNA double-strand break repair in human somatic cells. by Farjana Fattah, Eu Han Lee, Natalie Weisensel, Yongbao Wang, Natalie Lichter, Eric A Hendrickson

“…Mammals have evolved at least two genetically discrete ways to mediate DNA DSB repair: homologous recombination (HR) and non-homologous end joining (NHEJ). …”
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Sir4 Deficiency Reverses Cell Senescence by Sub-Telomere Recombination by Jun Liu, Xiaojing Hong, Lihui Wang, Chao-Ya Liang, Jun-Ping Liu

“…Here, we report that the sub-telomere regions facilitate telomere lengthening by homologous recombination, thereby attenuating senescence in yeast <i>Saccharomyces cerevisiae</i>. …”
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Characterization of the past and current duplication activities in the human 22q11.2 region by Morrow Bernice, Freyer Laina, Guo Xingyi, Zheng Deyou

“…<p>Abstract</p> <p>Background</p> <p>Segmental duplications (SDs) on 22q11.2 (LCR22), serve as substrates for meiotic non-allelic homologous recombination (NAHR) events resulting in several clinically significant genomic disorders.…”
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The Impact of Single- and Double-Strand DNA Breaks in Human Spermatozoa on Assisted Reproduction by Ashok Agarwal, Cătălina Barbăroșie, Rafael Ambar, Renata Finelli

“…In sperm, SSBs are irreversible, whereas DSBs can be repaired by homologous recombination, non-homologous end joining (NHEJ) and alternative NHEJ pathways. …”
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Dissection of zebrafish shha function using site-specific targeting with a Cre-dependent genetic switch by Kotaro Sugimoto, Subhra P Hui, Delicia Z Sheng, Kazu Kikuchi

“…In this study, we generated Zwitch, a Cre-dependent invertible gene-trap cassette, enabling the establishment of conditional alleles in zebrafish by generating intronic insertions via in vivo homologous recombination. To demonstrate the utility of Zwitch, we generated a conditional sonic hedgehog a (shha) allele. …”
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Imatinib enchances the sensitivity of gastrointestinal stromal tumors to topoisomerase II inhibitors by S. V. Boichuk, A. R. Galembikova, B. R. Ramazanov, A. Duensing

“…This might be due to reduced ability of GISTs to repair DNA damage by homologous recombination. Imatinib-induced reduction of Rad51 recombinase might be due to increased proteasome-dependent degradation.Conclusion. …”
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Roles of SETD2 in Leukemia—Transcription, DNA-Damage, and Beyond by Anna Skucha, Jessica Ebner, Florian Grebien

“…Presence of the H3K36me3 histone mark across the genome has been correlated with transcriptional activation and elongation, but also with the regulation of DNA mismatch repair, homologous recombination and alternative splicing. The role of SETD2 and the H3K36me3 histone mark in cancer is controversial. …”
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DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells. by Hicham Saad, Franck Gallardo, Mathieu Dalvai, Nicolas Tanguy-le-Gac, David Lane, Kerstin Bystricky

“…The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. …”
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High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast by Yi Yin, Margaret Dominska, Eunice Yim, Thomas D Petes

“…In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. …”
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Multiple forms of atypical rearrangements generating supernumerary derivative chromosome 15 by Sigman Marian, Dorrani Naghmeh, Malone Barbara M, Driscoll Jennette, Thatcher Karen N, Parokonny Alexander S, Wang Nicholas J, LaSalle Janine M, Schanen N Carolyn

“…Similar to the deletions found in most cases of Angelman and Prader Willi syndrome, the duplications appear to be mediated by unequal homologous recombination involving low copy repeats (LCR) that are found clustered in the region. …”
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BRCA1 deficiency in triple-negative breast cancer: Protein stability as a basis for therapy by Eun Choi, Gil-im Mun, Joohyun Lee, Hanhee Lee, Jaeho Cho, Yun-Sil Lee

“…Many aspects of this multifunctional protein have been revealed, including its essential role in homologous recombination repair, E3 ubiquitin ligase activity, transcriptional regulation, and apoptosis. …”
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An Introduced RNA-Only Approach for Plasmid Curing via the CRISPR-Cpf1 System in <i>Saccharomyces cerevisiae</i> by Bo-Chou Chen, Yu-Zhen Chen, Huan-Yu Lin

“…Based on our previous CRISPR-Cpf1 genome-editing system in <i>Saccharomyces cerevisiae</i>, the crRNA, designed for the replication origin of the CRISPR-Cpf1 plasmid, and the ssDNA, as a template for homologous recombination, were introduced for plasmid curing. …”
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Inactivation of the SGS1 and EXO1 Genes Synergistically Stimulates Plasmid Integration in Yeast by Anamarija Štafa, Ivan-Krešimir Svetec, Zoran Zgaga

“…Different procedures used for targeted genetic manipulations are based on homologous recombination between chromosomal and exogenous DNA. …”
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Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous <i>Streptomyces</i> Hosts by Xuejin Zhao, Yeqing Zong, Weijia Wei, Chunbo Lou

“…Then, we present an efficient, markerless and multiplex large gene cluster editing method based on in vitro CRISPR/Cas9 digestion and yeast homologous recombination. With this method, we successfully engineered the thaxtomin A cluster by simultaneously replacing the native promoters of the <i>txtED</i> operon, <i>txtABH</i> operon and <i>txtC</i> gene with strong constitutive promoters, and the yield of thaxtomin A improved to 289.5 µg/mL in heterologous <i>Streptomyces coelicolor</i> M1154. …”
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Comparison of Intraplasmid Rearrangements in Agrobacterium tumefaciens and Escherichia coli by Luka Bočkor, Srećko Jelenić†, Nenad Malenica, Jelena Mlinarec, Višnja Besendorfer, Ivana Ivančić-Baće

“…These two copies share a 570-bp region of homology and are separated by the ampicillin resistance gene. Homologous recombination between repeated copies of incomplete spectinomycin resistance genes results in the restoration of spectinomycin resistance. …”
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