Genetic analysis of DNA double-strand break mis-repair mechanisms using the human endogenous HPRT gene by Ahrabi, S

“…<p>DNA double strand breaks (DSBs) are the most lethal radiation-induced lesions in response to which cells employ either the error free homologous recombination (HR) repair pathway or error prone mechanisms, such as non homologous end joining (NHEJ) and microhomology- mediated end joining (MMEJ). …”

Phylogenetic analysis reveals that ERVs "Die Young" but HERV-H Is unusually conserved by Gemmell, P, Hein, J, Katzourakis, A

“…ERVs are commonly found in two forms, the full-length proviral form, and the more numerous solo-LTR form, thought to result from homologous recombination events. Here we introduce a phylogenetic framework to study ERV insertion and solo-LTR formation. …”

Feeding-related traits are affected by fosage of the foraging gene in Drosophila melanogaster. by Allen, A, Anreiter, I, Neville, M, Sokolowski, M

“…Before foraging's functions can be further dissected, we need a precise genetic null mutant to definitively map its amorphic phenotypes. We used homologous recombination to precisely delete foraging, generating the for0 null allele, and used recombineering to reintegrate a full copy of the gene, generating the {forBAC} rescue allele. …”

Radiation induced DNA DSBs: Contribution from stalled replication forks? by Harper, J, Anderson, J, O'Neill, P

“…Using gammaH2AX as a marker for DSB formation and RAD51 as a marker of homologous recombination (HR) which is recruited in the processing of frank DSBs or DSBs arising from stalled replication forks, we have investigated the contribution of SSBs and non-DSB DNA damage to the induction of DSBs in mammalian cells by ionizing radiation during the cell cycle. …”

A genomic view of experimental intraspecies and interspecies transformation of a rifampicin-resistance allele into Neisseria meningitidis by Alfsnes, K, Frye, SA, Eriksson, J, Eldholm, V, Bynildsrud, O, Bohlin, J, Harrison, OB, Hood, DW, Maiden, MCJ, Tonjum, T, Ambur, OH

“…Furthermore, the identification of recombination following experimental transformation of homologous alleles requires identifiable differences between donor and recipient, which in itself influences the propensity for homologous recombination (HR). This study identifies the distribution of HR events following intraspecies and interspecies Mc transformations of rpoB alleles encoding rifampicin resistance by whole-genome DNA sequencing and single nucleotide variant analysis. …”

BRN2 suppresses apoptosis, reprograms DNA damage repair, and is associated with a high somatic mutation burden in melanoma by Herbert, K, Binet, R, Lambert, J-P, Louphrasitthiphol, P, Kalkavan, H, Sanz, LS, Robles-Espinoza, CD, Sarkar, S, Suer, E, Andrews, S, Chauhan, J, Roberts, ND, Middleton, M, Gingras, A-C, Masson, J-Y, Larue, L, Falletta, P, Goding, C

“…Rapid PARP1-dependent BRN2 association with sites of DNA damage facilitates recruitment of Ku80 and reprograms DNA damage repair by promoting Ku-dependent nonhomologous end-joining (NHEJ) at the expense of homologous recombination. BRN2 also suppresses an apoptosis-associated gene expression program to protect against UVB-, chemotherapy- and vemurafenib-induced apoptosis. …”

Construction of Opa-positive and Opa-negative strains of Neisseria meningitidis to evaluate a novel meningococcal vaccine by Sadarangani, M, Hoe, J, Callaghan, M, Jones, C, Chan, H, Makepeace, K, Daniels-Treffandier, H, Deadman, M, Bayliss, C, Feavers, I, Van Der Ley, P, Pollard, A

“…The transformations demonstrated that homologous recombination of exogenous DNA into the meningococcal chromosome can occur with as little as 80 bp, and that minor sequence differences are permissible. …”

Recombinational switching of the Clostridium difficile S-layer and a novel glycosylation gene cluster revealed by large-scale whole-genome sequencing. by Dingle, K, Didelot, X, Ansari, M, Eyre, D, Vaughan, A, Griffiths, D, Ip, C, Batty, E, Golubchik, T, Bowden, R, Jolley, K, Hood, D, Fawley, W, Walker, A, Peto, T, Wilcox, M, Crook, D

“…These genes formed a 10-kb cassette, of which 12 divergent variants were found. Homologous recombination involving this cassette caused it to associate randomly with genotype. …”

Identification and localization of a methyl-accepting chemotaxis protein in Rhodobacter sphaeroides. by Wadhams, G, Martin, A, Armitage, J

“…The gene encoding the C-terminal McpG-GFP fusion was introduced by homologous recombination into the chromosome, replacing the wild-type gene. …”

Ancient protostome origin of chemosensory ionotropic glutamate receptors and the evolution of insect taste and olfaction by Croset, V, Rytz, R, Cummins, S, Budd, A, Brawand, D, Kaessmann, H, Gibson, T, Benton, R

“…Examination of IR gene structure and genomic distribution suggests both non-allelic homologous recombination and retroposition contributed to the expansion of this multigene family. …”

BRCA2 BRC motifs bind RAD51-DNA filaments. by Galkin, V, Esashi, F, Yu, X, Yang, S, West, S, Egelman, E

“…BRCA2 has been shown to interact directly with RAD51, an essential component of the cellular machinery for homologous recombination and the maintenance of genome stability. …”

DNA damage-induced interaction between a lineage addiction oncogenic transcription factor and the MRN complex shapes a tissue-specific DNA Damage Response and cancer predisposition by Binet, R, Lambert, J-P, Tomkova, M, Tischfield, S, Baggiolini, A, Picaud, S, Sarkar, S, Louphrasitthiphol, P, Dias, D, Carreira, S, Humphrey, T, Fillipakopoulos, P, White, R, Goding, CR

“…Consequently, cells with high MITF levels accumulate stalled replication forks, and display defects in homologous recombination-mediated repair associated with impaired MRN recruitment to DNA damage. …”

Radiosensitisation of bladder cancer cells by panobinostat is modulated by Ku80 expression. by Groselj, B, Kerr, M, Kiltie, A

“…Histone deacetylase inhibitors radiosensitise tumour cells but not normal cells in vitro and variously downregulate DNA damage signalling, homologous recombination (HR) and non-homologous end-joining (NHEJ) repair proteins. …”

BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair. by Chapman, R, Sossick, A, Boulton, S, Jackson, S

“…Our data suggest that the BRCA1-associated IRIF core corresponds to chromatin regions associated with repair by homologous recombination, and the enrichment of BRCA1 in IRIF represents a temporal switch in the DNA repair program. …”

Klebsiella quasipneumoniae provides a window into carbapenemase gene transfer, plasmid rearrangements, and patient interactions with the hospital environment by Mathers, AJ, Crook, D, Vaughan, A, Barry, KE, Vegesana, K, Stoesser, N, Parikh, HI, Sebra, R, Kotay, S, Walker, AS, Sheppard, AE

“…Longitudinal analysis of this strain demonstrated the acquisition of two unique blaKPC plasmids and then subsequent within-strain genetic rearrangement through transposition and homologous recombination. Our analysis highlights the apparent molecular propensity of K. quasipneumoniae to persist in the environment as well as acquire carbapenemase plasmids from other species and enabled an assessment of the genetic rearrangements which may facilitate horizontal transmission of carbapenemases.…”

DNA repair and cancer : from bench to clinic / by Wilson, David M., 1967- 545425, Madhusudan, Srinivasan 545424

Evolutionary Origin of Human <i>PALB2</i> Germline Pathogenic Variants by Jia Sheng Chian, Jiaheng Li, San Ming Wang

“…<i>PALB2</i> (Partner and localizer of BRCA2) is crucial for repairing DNA double-stranded breaks (DSBs) through homologous recombination (HR). Germline pathogenic variation in <i>PALB2</i> disrupts DNA damage repair and increases the risk of Fanconi Anemia, breast cancer, and ovarian cancer. …”
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Genome Sequence of <i>Eubacterium limosum</i> B2 and Evolution for Growth on a Mineral Medium with Methanol and CO₂ as Sole Carbon Sources by Guillaume Pregnon, Nigel P. Minton, Philippe Soucaille

“…Furthermore, a homologous recombination was found in subunit S of the type I restriction-modification system between both strains, changing the structure of the subunit, its sequence recognition and the methylome of the evolved clone. …”
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Development of a Replication-Deficient Bacteriophage Reporter Lacking an Essential Baseplate Wedge Subunit by Jose Gil, John Paulson, Henriett Zahn, Matthew Brown, Minh M. Nguyen, Stephen Erickson

“…In this study, homologous recombination was used to replace the expression of an essential baseplate wedge subunit (gp141) in SEA1 with a luciferase, NanoLuc^®. …”
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The SMC5/6 Complex Represses the Replicative Program of High-Risk Human Papillomavirus Type 31 by Ryan T. Gibson, Elliot J. Androphy

“…SMC5/6 is essential for homologous recombination DNA repair and functions as an antiviral factor during hepatitis B (HBV) and herpes simplex-1 (HSV-1) viral infections. …”
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