-
1341
Genetic analysis of DNA double-strand break mis-repair mechanisms using the human endogenous HPRT gene
Published 2017“…<p>DNA double strand breaks (DSBs) are the most lethal radiation-induced lesions in response to which cells employ either the error free homologous recombination (HR) repair pathway or error prone mechanisms, such as non homologous end joining (NHEJ) and microhomology- mediated end joining (MMEJ). …”
Thesis -
1342
Phylogenetic analysis reveals that ERVs "Die Young" but HERV-H Is unusually conserved
Published 2016“…ERVs are commonly found in two forms, the full-length proviral form, and the more numerous solo-LTR form, thought to result from homologous recombination events. Here we introduce a phylogenetic framework to study ERV insertion and solo-LTR formation. …”
Journal article -
1343
Feeding-related traits are affected by fosage of the foraging gene in Drosophila melanogaster.
Published 2017“…Before foraging's functions can be further dissected, we need a precise genetic null mutant to definitively map its amorphic phenotypes. We used homologous recombination to precisely delete foraging, generating the for0 null allele, and used recombineering to reintegrate a full copy of the gene, generating the {forBAC} rescue allele. …”
Journal article -
1344
Radiation induced DNA DSBs: Contribution from stalled replication forks?
Published 2010“…Using gammaH2AX as a marker for DSB formation and RAD51 as a marker of homologous recombination (HR) which is recruited in the processing of frank DSBs or DSBs arising from stalled replication forks, we have investigated the contribution of SSBs and non-DSB DNA damage to the induction of DSBs in mammalian cells by ionizing radiation during the cell cycle. …”
Journal article -
1345
A genomic view of experimental intraspecies and interspecies transformation of a rifampicin-resistance allele into Neisseria meningitidis
Published 2018“…Furthermore, the identification of recombination following experimental transformation of homologous alleles requires identifiable differences between donor and recipient, which in itself influences the propensity for homologous recombination (HR). This study identifies the distribution of HR events following intraspecies and interspecies Mc transformations of rpoB alleles encoding rifampicin resistance by whole-genome DNA sequencing and single nucleotide variant analysis. …”
Journal article -
1346
BRN2 suppresses apoptosis, reprograms DNA damage repair, and is associated with a high somatic mutation burden in melanoma
Published 2019“…Rapid PARP1-dependent BRN2 association with sites of DNA damage facilitates recruitment of Ku80 and reprograms DNA damage repair by promoting Ku-dependent nonhomologous end-joining (NHEJ) at the expense of homologous recombination. BRN2 also suppresses an apoptosis-associated gene expression program to protect against UVB-, chemotherapy- and vemurafenib-induced apoptosis. …”
Journal article -
1347
Construction of Opa-positive and Opa-negative strains of Neisseria meningitidis to evaluate a novel meningococcal vaccine
Published 2012“…The transformations demonstrated that homologous recombination of exogenous DNA into the meningococcal chromosome can occur with as little as 80 bp, and that minor sequence differences are permissible. …”
Journal article -
1348
Recombinational switching of the Clostridium difficile S-layer and a novel glycosylation gene cluster revealed by large-scale whole-genome sequencing.
Published 2013“…These genes formed a 10-kb cassette, of which 12 divergent variants were found. Homologous recombination involving this cassette caused it to associate randomly with genotype. …”
Journal article -
1349
Identification and localization of a methyl-accepting chemotaxis protein in Rhodobacter sphaeroides.
Published 2000“…The gene encoding the C-terminal McpG-GFP fusion was introduced by homologous recombination into the chromosome, replacing the wild-type gene. …”
Journal article -
1350
Ancient protostome origin of chemosensory ionotropic glutamate receptors and the evolution of insect taste and olfaction
Published 2010“…Examination of IR gene structure and genomic distribution suggests both non-allelic homologous recombination and retroposition contributed to the expansion of this multigene family. …”
Journal article -
1351
BRCA2 BRC motifs bind RAD51-DNA filaments.
Published 2005“…BRCA2 has been shown to interact directly with RAD51, an essential component of the cellular machinery for homologous recombination and the maintenance of genome stability. …”
Journal article -
1352
DNA damage-induced interaction between a lineage addiction oncogenic transcription factor and the MRN complex shapes a tissue-specific DNA Damage Response and cancer predisposition
Published 2023“…Consequently, cells with high MITF levels accumulate stalled replication forks, and display defects in homologous recombination-mediated repair associated with impaired MRN recruitment to DNA damage. …”
Journal article -
1353
Radiosensitisation of bladder cancer cells by panobinostat is modulated by Ku80 expression.
Published 2013“…Histone deacetylase inhibitors radiosensitise tumour cells but not normal cells in vitro and variously downregulate DNA damage signalling, homologous recombination (HR) and non-homologous end-joining (NHEJ) repair proteins. …”
Journal article -
1354
BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair.
Published 2012“…Our data suggest that the BRCA1-associated IRIF core corresponds to chromatin regions associated with repair by homologous recombination, and the enrichment of BRCA1 in IRIF represents a temporal switch in the DNA repair program. …”
Journal article -
1355
Klebsiella quasipneumoniae provides a window into carbapenemase gene transfer, plasmid rearrangements, and patient interactions with the hospital environment
Published 2019“…Longitudinal analysis of this strain demonstrated the acquisition of two unique blaKPC plasmids and then subsequent within-strain genetic rearrangement through transposition and homologous recombination. Our analysis highlights the apparent molecular propensity of K. quasipneumoniae to persist in the environment as well as acquire carbapenemase plasmids from other species and enabled an assessment of the genetic rearrangements which may facilitate horizontal transmission of carbapenemases.…”
Journal article -
1356
-
1357
Evolutionary Origin of Human <i>PALB2</i> Germline Pathogenic Variants
Published 2023-07-01“…<i>PALB2</i> (Partner and localizer of BRCA2) is crucial for repairing DNA double-stranded breaks (DSBs) through homologous recombination (HR). Germline pathogenic variation in <i>PALB2</i> disrupts DNA damage repair and increases the risk of Fanconi Anemia, breast cancer, and ovarian cancer. …”
Get full text
Article -
1358
Genome Sequence of <i>Eubacterium limosum</i> B2 and Evolution for Growth on a Mineral Medium with Methanol and CO<sub>2</sub> as Sole Carbon Sources
Published 2022-09-01“…Furthermore, a homologous recombination was found in subunit S of the type I restriction-modification system between both strains, changing the structure of the subunit, its sequence recognition and the methylome of the evolved clone. …”
Get full text
Article -
1359
Development of a Replication-Deficient Bacteriophage Reporter Lacking an Essential Baseplate Wedge Subunit
Published 2023-12-01“…In this study, homologous recombination was used to replace the expression of an essential baseplate wedge subunit (gp141) in SEA1 with a luciferase, NanoLuc<sup>®</sup>. …”
Get full text
Article -
1360
The SMC5/6 Complex Represses the Replicative Program of High-Risk Human Papillomavirus Type 31
Published 2020-09-01“…SMC5/6 is essential for homologous recombination DNA repair and functions as an antiviral factor during hepatitis B (HBV) and herpes simplex-1 (HSV-1) viral infections. …”
Get full text
Article