Chl1, an ATP-Dependent DNA Helicase, Inhibits DNA:RNA Hybrids Formation at DSB Sites to Maintain Genome Stability in <i>S. pombe</i> by Deyun He, Zhen Du, Huiling Xu, Xiaoming Bao

“…Mutation of Chl1 strongly impairs DSB repair capacity by homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways, and deleting RNase H further reduces DNA repair efficiency, which indicates that the enzymatic activities of Chl1 are needed in <i>Schizosaccharomyces pombe</i>. …”
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Phosphorylation of BRCA1 by ATM upon double-strand breaks impacts ATM function in end-resection: A potential feedback loop by Leilei Qi, Reka Chakravarthy, Monica M. Li, Chu-Xia Deng, Rong Li, Yanfen Hu

“…Summary: BRCA1 maintains genome stability by promoting homologous recombination (HR)-mediated DNA double-strand break (DSB) repair. …”
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DNA Double-Strand Breaks as Pathogenic Lesions in Neurological Disorders by Vincent E. Provasek, Joy Mitra, Vikas H. Malojirao, Muralidhar L. Hegde

“…Mammalian cells engage with several different strategies to recognize and repair chromosomal DSBs based on the cellular context and cell cycle phase, including homologous recombination (HR)/homology-directed repair (HDR), microhomology-mediated end-joining (MMEJ), and the classic non-homologous end-joining (NHEJ). …”
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PRMT7 Inhibitor SGC8158 Enhances Doxorubicin-Induced DNA Damage and Its Cytotoxicity by Ahyeon Jeong, Yena Cho, Minkyeong Cho, Gyu-Un Bae, Dae-Geun Song, Su-Nam Kim, Yong Kee Kim

“…In addition, PRMT7 activity is closely associated with DNA repair pathways, including both homologous recombination and non-homologous end-joining. Interestingly, SGC8158, in combination with doxorubicin, led to a synergistic increase in both DNA damage and cytotoxicity in MCF7 cells. …”
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Targeting PARP1 to Enhance Anticancer Checkpoint Immunotherapy Response: Rationale and Clinical Implications by Carlos Wagner S. Wanderley, Carlos Wagner S. Wanderley, Tatiana Strava Correa, Mariana Scaranti, Fernando Queiroz Cunha, Fernando Queiroz Cunha, Romualdo Barroso-Sousa

“…PARPi impairs single-strand break DNA repair; this mechanism involves synthetic lethality in tumor cells with deficient homologous recombination. More recently, novel evidence indicated that PAPRi has the potential to modulate the antitumor immune response by activating antigen-presenting cells, infiltrating effector lymphocytes, and upregulating programmed death ligand-1 in tumors. …”
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Different Mechanisms in Doxorubicin-Induced Cardiomyopathy: Impact of BRCA1 and BRCA2 Mutations by Hien C. Nguyen, Jefferson C. Frisbee, Krishna K. Singh

“…The BRCA1 and BRCA2 proteins are distinct as they perform crucial yet separate roles in the homologous recombination repair of DNA double-strand breaks, thereby maintaining genomic integrity. …”
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O-GlcNAcylation Affects the Pathway Choice of DNA Double-Strand Break Repair by Sera Averbek, Burkhard Jakob, Marco Durante, Nicole B. Averbeck

“…We confirmed that O-GlcNAcylation of MDC1 is increased upon irradiation and identified additional repair factors related to Homologous Recombination (HR), CtIP and BRCA1, which were increasingly O-GlcNAcyated upon irradiation. …”
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Ablation of the Sam68 RNA Binding Protein Protects Mice from Age-Related Bone Loss.

“…Herein we report the generation of Sam68-null mice by homologous recombination. Aged Sam68 mice preserved their bone mass, in sharp contrast with 12-month-old wild-type littermates in which bone mass was decreased up to approximately 75%. …”
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Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster by Zoltán G. Páhi, Levente Kovács, Diána Szűcs, Barbara N. Borsos, Péter Deák, Tibor Pankotai

“…Furthermore, the Drosophila DR-white assay demonstrated that homologous recombination does not occur in the absence of Usp34, indicating an indispensable role of Usp34 in this process.…”
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Generating endogenous Myh11-driven Cre mice for sex-independent gene deletion in smooth muscle cells by Yang Zhao, Guizhen Zhao, Ziyi Chang, Tianqing Zhu, Ying Zhao, Haocheng Lu, Chao Xue, Thomas L. Saunders, Yanhong Guo, Lin Chang, Y. Eugene Chen, Jifeng Zhang

“…We used CRISPR/Cas9-mediated homologous recombination between a donor vector carrying the CreNLSP2A or CreERT2–P2A sequence and homologous arm surrounding the translation start site of the Myh11 gene to generate Cre-knockin mice. …”
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The HSV-1 exonuclease, UL12, stimulates recombination by a single strand annealing mechanism. by April J Schumacher, Kareem N Mohni, Yinan Kan, Eric A Hendrickson, Jeremy M Stark, Sandra K Weller

“…Single strand annealing (SSA) was increased in HSV-infected cells, while homologous recombination (HR), non-homologous end joining (NHEJ) and alternative non-homologous end joining (A-NHEJ) were decreased. …”
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DNA Damage Repair: Predictor of Platinum Efficacy in Ovarian Cancer? by Dimitra T. Stefanou, Vassilis L. Souliotis, Roubini Zakopoulou, Michalis Liontos, Aristotelis Bamias

“…Various DNA repair molecular components were found deregulated in ovarian cancer, including molecules involved in homologous recombination repair (HRR), nucleotide excision repair (NER), mismatch repair (MMR), non-homologous end-joining (NHEJ), and base excision repair (BER), which can be possibly exploited as novel therapeutic targets and sensitive/effective biomarkers. …”
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Creation and Characterization of Mycolicibacterium Smegmatis mc2155 with Deletions in Genes Encoding Sterol Oxidation Enzymes by Byakov Artem, Karpov Mikhail, Strizhov Nikolai, Donova Marina

“…A mutant strain of M. smegmatis with deletions in three genes (MSMEG_1604, MSMEG_5228 and MSMEG_5233) encoding known enzymes exhibiting 3β-hydroxysteroid dehydrogenase activity was constructed by homologous recombination coupled with double selection. The resulting mutant retained macromorphological properties and the ability to convert cholesterol. 3-Keto-4-en-steroids were found among the sterol catabolism intermediates. …”
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Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method by Ryo Ezaki, Kennosuke Ichikawa, Mei Matsuzaki, Hiroyuki Horiuchi

“…Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. …”
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Application of CRISPR/Cas9 for Rapid Genome Editing of Pseudorabies Virus and Bovine Herpesvirus-1 by Wanqi Yu, Jingyi Liu, Yingnan Liu, Maria Forlenza, Hongjun Chen

“…Upon the cleavage of the TK or gIgE gene by Cas9 protein, this was replaced by the <i>enhanced green fluorescence protein</i> (<i>eGFP</i>) by homologous recombination. With this approach, we generated recombinant TK-/eGFP+ PRV and gIgE-/eGFP+ BHV-1 mutants and then proceeded to characterize their biological activities in vitro and in vivo. …”
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Biomarkers beyond BRCA: promising combinatorial treatment strategies in overcoming resistance to PARP inhibitors by Yu-Yi Chu, Clinton Yam, Hirohito Yamaguchi, Mien-Chie Hung

“…Abstract Poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi) exploit the concept of synthetic lethality and offer great promise in the treatment of tumors with deficiencies in homologous recombination (HR) repair. PARPi exert antitumor activity by blocking Poly(ADP-ribosyl)ation (PARylation) and trapping PARP1 on damaged DNA. …”
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Mutational signature dynamics shaping the evolution of oesophageal adenocarcinoma by Sujath Abbas, Oriol Pich, Ginny Devonshire, Shahriar A. Zamani, Annalise Katz-Summercorn, Sarah Killcoyne, Calvin Cheah, Barbara Nutzinger, Nicola Grehan, Nuria Lopez-Bigas, OCCAMS Consortium, Rebecca C. Fitzgerald, Maria Secrier

“…We also identify prevalent alterations in DNA damage repair pathways, with homologous recombination, base and nucleotide excision repair and translesion synthesis mutated in up to 50% of the cohort, and surprisingly uncoupled from transcriptional activity. …”
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Multichromosomal Mitochondrial Genome of <i>Paphiopedilum micranthum</i>: Compact and Fragmented Genome, and Rampant Intracellular Gene Transfer by Jia-Xing Yang, Nicolas Dierckxsens, Ming-Zhu Bai, Yan-Yan Guo

“…We suggest that repeat-mediated homologous recombination enables the dynamic structure of mitochondrial genomes in Orchidaceae.…”
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The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts by Aleix Bayona-Feliu, Emilia Herrera-Moyano, Nibal Badra-Fajardo, Iván Galván-Femenía, María Eugenia Soler-Oliva, Andrés Aguilera

“…Strikingly, TRC regions show increased mutagenesis in cancer cells with signatures of homologous recombination deficiency, transcription-coupled nucleotide excision repair (TC-NER) and of the AID/APOBEC cytidine deaminases, being predominant at head-on collisions. …”
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Post-Translational Modifications of PCNA: Guiding for the Best DNA Damage Tolerance Choice by Gemma Bellí, Neus Colomina, Laia Castells-Roca, Neus P. Lorite

“…In contrast, PCNA sumoylation impedes the homologous recombination (HR)-mediated salvage recombination (SR) repair pathway. …”
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