An efficient CRISPR/Cas9‐based genome editing system for alkaliphilic Bacillus sp. N16‐5 and application in engineering xylose utilization for D‐lactic acid production by Shiyong Huang, Yanfen Xue, Cheng Zhou, Yanhe Ma

“…N16‐5, an industrially interesting strain with great potential for the production of lactic acid and alkaline polysaccharide hydrolases, can only be engineered genetically by the laborious and time‐consuming homologous recombination. In this study, we reported the successful development of a CRISPR/Cas9‐based genome editing system with high efficiency for single‐gene deletion, large gene fragment deletion and exogenous DNA chromosomal insertion. …”
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The genomic stability regulator PTIP is required for proper chromosome segregation in mitosis by Fengxia Zhang, Mingxuan Wei, Haoran Chen, Liting Ji, Yan Nie, Jungseog Kang

“…It binds to 53BP1 and antagonizes inappropriate homologous recombination for a proper DNA damage response. …”
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Tumor cell death mediated by peptides that recognize branched intermediates of DNA replication and repair. by Mamon Dey, Sukanya Patra, Leo Y Su, Anca M Segall

“…Herein we investigated the effect of a structure-selective d-amino acid peptide wrwycr that binds replication fork mimics and Holliday Junction (HJs) intermediates of homologous recombination (HR) in vitro, and inhibits their resolution by HJ-processing enzymes. …”
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The Influence of Edaphic Factors on DNA Damage and Repair in Wild Wheat <i>Triticum dicoccoides</i> Körn. (<i>Poaceae</i>, Triticeae) by Olga Raskina, Boris Shklyar, Eviatar Nevo

“…Finally, RAD51 and LigIV foci on chromosomes indicate ongoing DSB repair during the M-phase via the Homologous Recombination and Non-Homologous End Joining pathways. …”
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Computational Insights into the Dynamic Structural Features and Binding Characteristics of Recombinase UvsX Compared with RecA by Yue Pan, Ningkang Xie, Xin Zhang, Shuo Yang, Shaowu Lv

“…RecA family recombinases are the core enzymes in the process of homologous recombination, and their normal operation ensures the stability of the genome and the healthy development of organisms. …”
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Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit by Markus Engstler, Tom Beneke

“…Since our method does not require DNA double-strand breaks, homologous recombination, donor DNA, or isolation of clones, we believe that this enables for the first time functional genetic screens in Leishmania via delivery of plasmid libraries.…”
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Novel Centromeric Loci of the Wine and Beer Yeast Dekkera bruxellensis CEN1 and CEN2. by Olena P Ishchuk, Tanja Vojvoda Zeljko, Anna J Schifferdecker, Sofia Mebrahtu Wisén, Åsa K Hagström, Elżbieta Rozpędowska, Mikael Rørdam Andersen, Linda Hellborg, Zhihao Ling, Andrei A Sibirny, Jure Piškur

“…The introduction of extra copies of CEN1 and CEN2 promotes both genome rearrangements and ploidy shifts, with these effects mediated by homologous recombination (between circular plasmid and genome centromere copy) or by chromosome breakage when integrated. …”
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SNP-VISTA: An interactive SNP visualization tool by Hugenholtz Philip, Pennacchio Len A, Minovitsky Simon, Teplitsky Michael V, Shah Nameeta, Hamann Bernd, Dubchak Inna L

“…Massive amounts of ecogenomics data for studying homologous recombination in microbial populations (EcoSNP-VISTA). …”
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Concomitant knockout of target and transporter genes in filamentous fungi by genome co‐editing by Koichi Tamano

“…This is probably because a self‐cloning strain is similar to a genetically modified strain, as both undergo homologous recombination, although exogenous genes are not introduced. …”
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Evolution and transmission of a conjugative plasmid encoding both ciprofloxacin and ceftriaxone resistance in Salmonella by Kaichao Chen, Edward Wai Chi Chan, Sheng Chen

“…These two daughter plasmids could be integrated into one single plasmid through ISPa40 mediated homologous recombination. Mouse infection and treatment experiments showed that carriage of plasmid, pSa44-CIP-CRO by S. typhimurium led to the impairment of treatment by ciprofloxacin or cefitiofur, a veterinary drug with similar properties as ceftriaxone. …”
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Treating ARID1A mutated cancers by harnessing synthetic lethality and DNA damage response by Jayaprakash Mandal, Prativa Mandal, Tian-Li Wang, Ie-Ming Shih

“…The recent success of PARP inhibitors in treating homologous recombination DNA repair-deficient tumors has engendered keen interest in developing synthetic lethality-based therapeutic strategies for ARID1A-mutated neoplasms. …”
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First insight into how stress exposure triggers Vibrio harveyi recipient successful conjugation by Yiqin Deng, Si’ao Gao, Liwen Xu, Changhong Cheng, Hongling Ma, Juan Feng

“…These results will help establish homologous recombination gene knockout technology and greatly advance molecular theoretical research on V. harveyi. …”
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Emerging systemic therapy options beyond CDK4/6 inhibitors for hormone receptor-positive HER2-negative advanced breast cancer by Jun Ma, Jack Junjie Chan, Ching Han Toh, Yoon-Sim Yap

“…Recent clinical trials have been directed at the targets and pathways implicated, including estrogen and androgen receptors, PI3K/AKT/mTOR and MAPK pathways, tyrosine kinase receptors such as FGFR and HER2, homologous recombination repair pathway, other components of the cell cycle and cell death. …”
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Spontaneous mutagenesis in human cells is controlled by REV1-Polymerase ζ and PRIMPOL by Zsolt Gyüre, Ádám Póti, Eszter Németh, Bernadett Szikriszt, Rita Lózsa, Michał Krawczyk, Andrea L. Richardson, Dávid Szüts

“…Using whole-genome sequencing of cultured human RPE-1 cell clones, we find that REV1 and Polymerase ζ are wholly responsible for one component of base substitution mutagenesis that resembles homologous recombination deficiency, whereas the remaining component that approximates oxidative mutagenesis is reduced in PRIMPOL−/− cells. …”
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Analysis of RecA-independent recombination events between short direct repeats related to a genomic island and to a plasmid in Escherichia coli K12 by María F. Azpiroz, Magela Laviña

“…This latter was searched for by means of a PCR approach to detect the island excision in E. coli K12 mutants affected in a number of recombination functions, including the 16 E. coli K12 site-specific recombinases, the RecET system, and multiple proteins that participate in the RecA-dependent pathways of homologous recombination. None of these appeared to be involved in the island excision. …”
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Establishment of RNA Interference Genetic Transformation System and Functional Analysis of FlbA Gene in <i>Leptographium qinlingensis</i> by Tian Gan, Huanli An, Ming Tang, Hui Chen

“…Using the RNA silencing vector pSilent-1 as a template, we established an RNA interference genetic transformation system mediated by <i>Agrobacterium tumefaciens</i> GV3101, which is suitable for the gene study for <i>Leptographium qinlingensis</i> by homologous recombination and strain interference system screening. …”
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Suppression of NBS1 Upregulates CyclinB to Induce Olaparib Sensitivity in Ovarian Cancer by Ailing Zhong MMed, Chien-shan Cheng MD, Ren quan Lu PhD, Lin Guo PhD

“…Results: Our findings suggest that NBS1 deficiency leads to defective homologous recombination repair. Inhibition of NBS1 expression activates CHK1 and CyclinB signaling pathways leading to cell cycle arrest and sensitizes ovarian cancer cells to Olaparib treatment in vitro and in vivo. …”
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CRISPR-Mediated Base Editing: From Precise Point Mutation to Genome-Wide Engineering in Nonmodel Microbes by Mengyuan Li, Yi-Xin Huo, Shuyuan Guo

“…This method is a fusion of impaired CRISPR/Cas9 nuclease and base deaminase, enabling the precise point mutation at the target without inducing homologous recombination. This review updates the latest advancement of base editors in microbes, including the conclusion of all microbes that have been researched by base editors, the introduction of newly developed base editors, and their applications. …”
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Protocol to measure end resection intermediates at sequence-specific DNA double-strand breaks by quantitative polymerase chain reaction using ER-AsiSI U2OS cells by Ajit K. Sharma, Amira Mohammed Fitieh, Jana Yasser Hafez Ali, Ismail Hassan Ismail

“…Summary: DNA end resection is a critical step in the homologous recombination pathway of repairing DNA double-strand breaks (DSBs) that can be visualized in cells by detecting the generation of single-stranded DNA (ssDNA) intermediates formed during the resection of the DSBs. …”
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Alteration of intestinal mucosal microbiota in mice with Chinese dampness-heat syndrom diarrhea by improper diet combined with high temperature and humidity environments by Bo Qiao, Xiaoya Li, Maijiao Peng, Huaying Hui, Zhoujin Tan

“…According to the functional predictive analysis, we found that Lactobacillus showed a significant negative correlation with Protein export, Homologous recombination, Phenylalanine, tyrosine, tryptophan biosynthesis, Citrate cycle, and Lipoic acid metabolism.ConclusionDiarrhea with DSH constructed under improper diet and HTH environment may be related to Lactobacillus and Haemophilus. …”
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