Investigating DNA double-strand break repair in Dictyostelium discoideum by Couto, C

“…This work has demonstrated the regulatory role of Adprt1a in DSB repair by promoting non-homologous end joining (NHEJ), at the expense of homologous recombination (HR). This may be achieved by the enhanced association of the Ku70/Ku80 heterodimer, the DSB sensor that initiates NHEJ, with chromatin following DNA damage in a PAR-dependent manner. …”

Evolutionary and molecular analysis of the emergent severe fever with thrombocytopenia syndrome virus. by Lam, T, Liu, W, Bowden, T, Cui, N, Zhuang, L, Liu, K, Zhang, Y, Cao, W, Pybus, O

“…Our discovery of a mosaic L segment sequence, which is descended from two major circulating lineages of SFTSV in China, represents the first evidence that homologous recombination plays a role in SFTSV evolution. Selection analyses indicate that negative selection is predominant in SFTSV genes, yet differences in selective forces among genes are consistent between Phlebovirus species. …”

Nested Russian doll-like genetic mobility drives rapid dissemination of the carbapenem resistance gene blaKPC by Sheppard, A, Stoesser, N, Wilson, D, Sebra, R, Kasarkis, A, Anson, L, Giess, A, Pankhurst, L, Vaughan, A, Grim, C, Cox, H, Yeh, A, Sifri, C, Walker, A, Peto, T, Crook, D, Mathers, A

“…We also identify a common insertion site for Tn4401 within various Tn2-like elements, suggesting that homologous recombination between Tn2-like elements has enhanced the spread of Tn4401 between different plasmid vectors. …”

Study of DNA double strand break repair in Dictyostelium discoideum by Lempidaki, S

“…The homologous recombination (HR) pathway contributes to genome integrity by mediating double strand break (DSB) repair using a homologous DNA sequence as a template. …”

KAT2-mediated acetylation switches the mode of PALB2 chromatin association to safeguard genome integrity by Fournier, M, Rodrigue, A, Milano, L, Bleuyard, J-Y, Couturier, AM, Wall, J, Ellins, J, Hester, S, Smerdon, SJ, Tora, L, Masson, J-Y, Esashi, F

“…The tumour suppressor PALB2 stimulates RAD51-mediated homologous recombination (HR) repair of DNA damage, whilst its steady-state association with active genes protects these loci from replication stress. …”

A study of the Bloom's syndrome protein by Karow, J

“…Most characteristically, these cells show elevated levels of sister-chromatid exchanges which probably result from homologous recombination events. Since the cells are not hypersensitive to DNA damaging agents, the defect is unlikely to be in one of the common DNA repair pathways. …”

A prototypical Fanconi anemia pathway in lower eukaryotes? by McHugh, P, Ward, T, Chovanec, M

“…Yeast cells require a combination of nucleotide excision repair, homologous recombination repair and postreplication repair/translesion DNA synthesis to remove ICLs. …”

Stability by multimer resolution of pJHCMW1 is due to the Tn1331 resolvase and not to the Escherichia coli Xer system. by Tolmasky, M, Colloms, S, Blakely, G, Sherratt, D

“…The Escherichia coli Xer site-specific recombination system acts at sites such as ColE1 cer to resolve plasmid multimers formed by homologous recombination, thereby maintaining plasmids in a monomeric state and helping to ensure stable plasmid inheritance. …”

Evidence for soft selective sweeps in the evolution of pneumococcal multidrug resistance and vaccine escape. by Croucher, N, Chewapreecha, C, Hanage, W, Harris, SR, McGee, L, van der Linden, M, Song, J, Ko, K, de Lencastre, H, Turner, C, Yang, F, Sá-Leão, R, Beall, B, Klugman, K, Parkhill, J, Turner, P, Bentley, S

“…The rapid diversification through homologous recombination seen in the global collection was similarly observed in the absence of vaccination in a set of isolates from the Maela refugee camp in Thailand, a collection that also allowed variation to be observed within carriage through longitudinal sampling. …”

Efficient second strand cleavage during Holliday junction resolution by RuvC requires both increased junction flexibility and an exposed 5' phosphate. by Osman, F, Gaskell, L, Whitby, M

“… BACKGROUND: Holliday junction (HJ) resolution is a critical step during homologous recombination. In Escherichia coli this job is performed by a member of the RNase H/Integrase superfamily called RuvC, whereas in Schizosaccharomyces pombe it has been attributed to the XPF family member Mus81-Eme1. …”

6-thioguanine selectively kills BRCA2-defective tumors and overcomes PARP inhibitor resistance. by Issaeva, N, Thomas, H, Djureinovic, T, Djurenovic, T, Jaspers, J, Stoimenov, I, Kyle, S, Pedley, N, Gottipati, P, Zur, R, Sleeth, K, Chatzakos, V, Mulligan, E, Lundin, C, Gubanova, E, Kersbergen, A, Harris, A, Sharma, R, Rottenberg, S, Curtin, N, Helleday, T

“…Familial breast and ovarian cancers are often defective in homologous recombination (HR) due to mutations in the BRCA1 or BRCA2 genes. …”

Contextual synthetic lethality of cancer cell kill based on the tumor microenvironment. by Chan, N, Pires, I, Bencokova, Z, Coackley, C, Luoto, K, Bhogal, N, Lakshman, M, Gottipati, P, Oliver, F, Helleday, T, Hammond, E, Bristow, R

“…Replicating cells exposed to either severe acute hypoxia (16 hours with 0.02% O(2)) followed by reoxygenation or moderate chronic hypoxia (72 hours with 0.2% O(2)) treatments have decreased homologous recombination (HR) protein expression and function. …”

Depletion of the type 1 IGF receptor delays repair of radiation-induced DNA double strand breaks by Turney, B, Kerr, M, Chitnis, M, Lodhia, K, Wang, Y, Riedemann, J, Rochester, M, Protheroe, A, Brewster, S, Macaulay, V

“…</p> <h4>Methods:</h4> <p>We tested effects of IGF-1R siRNA transfection on the repair of radiation-induced DSBs by immunoblotting and immunofluorescence for cH2AX, and pulsed-field gel electrophoresis. Homologous recombination (HR) was quantified by reporter assays, and cell cycle distribution by flow cytometry.…”

A large AZFc deletion removes DAZ3/DAZ4 and nearby genes from men in Y haplogroup N. by Fernandes, S, Paracchini, S, Meyer, L, Floridia, G, Tyler-Smith, C, Vogt, P

“…Furthermore, the observed deletions cannot be derived from the GenBank reference sequence by a single recombination event; an origin by homologous recombination from such a sequence organization must be preceded by an inversion event. …”

Evolution and population structure of Salmonella enterica serovar Newport. by Sangal, V, Harbottle, H, Mazzoni, C, Helmuth, R, Guerra, B, Didelot, X, Paglietti, B, Rabsch, W, Brisse, S, Weill, F, Roumagnac, P, Achtman, M

“…Both mutation and homologous recombination are responsible for diversification within each of these lineages, but the relative frequencies differed with the lineage. …”

Beyond the trinity of ATM, ATR, and DNA-PK: multiple kinases shape the DNA damage response in concert with RNA metabolism by Burger, K, Ketley, RF, Gullerova, M

“…The importance of kinase signaling and RNA processing in the DDR is highlighted by the regulation of p53-binding protein (53BP1), a key regulator of DSB repair pathway choice between homologous recombination (HR) and non-homologous end joining (NHEJ). …”

Replication fork collapse and recombination-dependent replication restart at replication fork barriers by Tamang, S

“…Replication fork collapse is a poorly understood process that is thought to involve either replisome remodelling and/or disassembly paving the way for homologous recombination (HR) proteins to act and restart replication, by a process known as recombination dependent replication restart (RDR), which is essential for completing DNA synthesis. …”

Rapid, DNA-induced subunit exchange by DNA gyrase by Germe, T, Bush, N, Baskerville, V, Saman, D, Benesch, J, Maxwell, A

“…This exchange does not require ATP, can occur in the presence of fluoroquinolones, and raises the possibility of non-homologous recombination solely through gyrase activity. The ability of gyrase to undergo subunit exchange also explains how gyrase heterodimers, containing a single active-site tyrosine, can carry out double-strand passage reactions.…”

Components of a Fanconi-like pathway control Pso2-independent DNA interstrand crosslink repair in yeast by Ward, T, Dudášová, Z, Sarkar, S, Bhide, MR, Vlasáková, D, Chovanec, M, McHugh, P

“…Moreover, ICL-induced Rad51 chromatin loading is delayed when both Pso2 and components of the Mph1-Mgm101-MutSα and Exo1 pathway are inactivated, demonstrating that the homologous recombination stages of ICL repair are inhibited. …”

Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration by Ding, Z, Babensee, J, Simon, S, Lu, H, Perrard, J, Bullard, D, Dai, X, Bromley, S, Dustin, M, Entman, M, Smith, C, Ballantyne, C

“…To differentiate the unique and overlapping functions of LFA-1 and Mac-1, LFA-1-deficient mice were developed by targeted homologous recombination in embryonic stem cells, and neutrophil function was compared in vitro and in vivo with Mac-1-deficient, CD18-deficient, and wild-type mice. …”